PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus RPL26 USP53 19615732 biogrid,intact exp P P P P P P P P P P P P P P P P P USP53 RAD50 19615732 biogrid,intact exp G GP G P GP G GP GP G GP GP GP G G GP GP G GP G GP G GP GP GP GP GP GP IREB2 USP53 21988832 biogrid,intact exp G GP G GP G GP GP G GP GP G G G GP GP G GP G GP G GP GP GP GP GP GP USP53 DDB2 23159851 biogrid exp G GP G P GP G G GP G GP GP GP G G GP GP G GP G GP G GP GP G GP GP ARG1 USP53 19615732 biogrid,intact exp G P G G GP G G G G G G TRAF2 USP53 16189514 hprd,biogrid,mint,intact exp P P P P P P P P P P P P P P P GP GP P BLMH USP53 19615732 biogrid,intact exp G G G G G G GP G GP G G G GP G G GP G G G G G GP G GP GP PSMA6 USP53 19615732 biogrid,intact exp P P P P P P P P P P P P P P P P P USP53 PVRL2 21988832 biogrid,intact exp G GP G P G G GP GP G GP GP GP G G GP G G GP G GP G GP GP GP GP GP GP USP53 CPNE7 19615732 biogrid,intact exp G GP G P GP G GP GP G GP GP GP G G GP GP G GP G GP G GP GP GP GP GP GP CAT USP53 19615732 biogrid,intact exp G GP G P G G G G G GP GP GP G G GP G G G G G G G G GP G G G TGM3 USP53 19615732 biogrid,intact exp G G GP G G G G GP G G G GP G G G GP G G G GP CSTA USP53 19615732 biogrid,intact exp G G P G G G GP GP G G G G GP G G G G GP GP GRB2 USP53 20936779 biogrid,i2d,intact exp G GP G P GP G G GP G GP GP GP G G GP GP G GP G GP G GP GP GP GP GP GP FLNC USP53 19615732 biogrid,intact exp G GP G GP G GP GP G GP G GP G G GP GP G GP G GP G GP GP GP G GP GP DAB1 USP53 25416956 biogrid,intact exp G G GP G GP G G G G G GP GP G G G GP GP G DSG1 USP53 19615732 biogrid,intact exp G P P P GP P P P P P P P P USP53 YAE1D1 19615732 biogrid,intact exp G GP G P GP G GP GP G GP GP GP G G GP GP G GP G GP G GP GP GP GP GP GP USP53 KRT77 19615732 biogrid,intact exp G G G G G G G G GP G G G G G G G G HCFC1 USP53 19615732 biogrid,intact exp G GP G P GP G GP G G GP GP GP G G G GP G G G G G GP GP G G GP GP ANXA2P2 USP53 19615732 intact exp PPP2R1B USP53 19615732 biogrid,intact exp G GP G P GP G GP GP G GP GP GP G G GP GP G GP G GP G GP GP GP GP GP GP AZGP1 USP53 19615732 biogrid,intact exp G G G G G GP G G G G G G G G GP G G G G CASP14 USP53 19615732 biogrid,intact exp G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q70EK8 Q92692 two hybrid 21988832 direct interaction X 2 Q70EK8 O75553-6 two hybrid 25416956 direct interaction X 2 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q70EK8 Q9UBL6 affinity chromatography technology 19615732 physical association X 2 Q70EK8 P62993-2 two hybrid 20936779 direct interaction X 2 P61254 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q92466 Q70EK8 two hybrid 23159851 direct interaction X 2 Q92466 Q70EK8 affinity chromatography technology 23159851 physical association X 2 P04040 Q70EK8 affinity chromatography technology 19615732 physical association X 2 B2CIS9 Q70EK8 affinity chromatography technology 19615732 physical association X 2 A0A024R8H5 Q70EK8 two hybrid 16189514 direct interaction X 2 Q02413 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q08188 Q70EK8 affinity chromatography technology 19615732 physical association X 2 P60900 Q70EK8 affinity chromatography technology 19615732 physical association X 2 P0CG48 Q70EK8 affinity chromatography technology 22118674 physical association X 2 P05089 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q7Z794 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q70EK8 Q14315 affinity chromatography technology 19615732 physical association X 2 Q70EK8 Q92878 affinity chromatography technology 19615732 physical association X 2 P51610 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q13867 Q70EK8 affinity chromatography technology 19615732 physical association X 2 Q9NRH1 Q70EK8 affinity chromatography technology 19615732 physical association X 2 P01040 Q70EK8 affinity chromatography technology 19615732 physical association X 2 D3DW85 Q70EK8 two hybrid 21988832 direct interaction X 2 P25311 Q70EK8 affinity chromatography technology 19615732 physical association X 2 A0A024R8H5 Q70EK8 two hybrid 16189514 - X 2 A0A024R8H5 Q70EK8 two hybrid 16189514 - X 2 Q70EK8 anti tag coimmunoprecipitation 19615732 physical association C 19 Q70EK8 Q8CZX0 two hybrid pooling approach 20711500 physical association X 2 Q70EK8 Q92692 two hybrid array 21988832 physical association X 2 A0A024R8H5 Q70EK8 two hybrid pooling approach 16189514 physical association X 2 B0LPF3 Q70EK8 two hybrid pooling approach 20936779 physical association X 2 Q70EK8 Q81QG7 two hybrid pooling approach 20711500 physical association X 2 P48200 Q70EK8 two hybrid array 21988832 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B IREB2 USP53 MI:0004(affinity chromatography technology)|MI:0397(two hybrid array) 21988832 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_288340|UniProt_117944 bait|unspecified role prey|unspecified role PSMA6 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119690 prey|prey bait|bait BLMH USP53 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119365|UniProt_100699 prey|prey bait|bait TRAF2 USP53 MI:0018(two hybrid)|MI:0096(pull down)|MI:0018(two hybrid) 16189514 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association)|- (biogrid)|(intact)|MI:0468(hprd) BIOGRID_78810|UniProt_151662|HPRD_21862 bait|unspecified role|- prey|unspecified role|- AZGP1 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119359 prey|prey bait|bait FLNC USP53 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119486|UniProt_100699 prey|prey bait|bait USP53 RAD50 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120127|UniProt_100699 bait|bait prey|prey USP53 PVRL2 MI:0004(affinity chromatography technology)|MI:0397(two hybrid array) 21988832 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_288441|UniProt_118051 prey|unspecified role bait|unspecified role USP53 DDB2 MI:0018(two hybrid)|MI:0018(two hybrid) 23159851 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_247812|BIOGRID_247809 bait|prey prey|bait TGM3 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119824 prey|prey bait|bait CSTA USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119422 prey|prey bait|bait DSG1 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119450 prey|prey bait|bait USP53 YAE1D1 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_120588 bait|bait prey|prey RPL26 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119778 prey|prey bait|bait CAT USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119380 prey|prey bait|bait CASP14 USP53 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120332|UniProt_100699 prey|prey bait|bait PPP2R1B USP53 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119675|UniProt_100699 prey|prey bait|bait GRB2 USP53 MI:0018(two hybrid)|MI:0096(pull down) 20936779 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_127117|UniProt_110498 bait|bait prey|prey USP53 KRT77 MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_120876 bait prey USP53 CPNE7 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120394|UniProt_100699 bait|bait prey|prey USP53 TRAF2 MI:0018(two hybrid)|MI:0018(two hybrid) 16189514 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) MI:0471(mint)|MI:0471(mint) MINT_66027|MINT_36867 prey|prey bait|bait UBC USP53 MI:0415(enzymatic study) 22118674 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_160587 bait prey HCFC1 USP53 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100699|BIOGRID_119513 prey|prey bait|bait ARG1 USP53 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119356|UniProt_100699 prey|prey bait|bait

PPI - HINT Gene A Gene B PMID Method Quality ANXA2P2 USP53 19615732,19615732 0004,0007 HT CSTA USP53 19615732,19615732 0004,0007 HT CAT USP53 19615732,19615732 0004,0007 HT ARG1 USP53 19615732,19615732 0004,0007 HT AZGP1 USP53 19615732,19615732 0004,0007 HT PPP2R1B USP53 19615732,19615732 0004,0007 HT CASP14 USP53 19615732,19615732 0004,0007 HT HCFC1 USP53 19615732,19615732 0004,0007 HT PSMA6 USP53 19615732,19615732 0004,0007 HT RPL26 USP53 19615732,19615732 0004,0007 HT DSG1 USP53 19615732,19615732 0004,0007 HT TGM3 USP53 19615732,19615732 0004,0007 HT BLMH USP53 19615732,19615732 0004,0007 HT FLNC USP53 19615732,19615732 0004,0007 HT USP53 KRT77 19615732,19615732 0004,0007 HT USP53 RAD50 19615732,19615732 0004,0007 HT USP53 YAE1D1 19615732,19615732 0004,0007 HT USP53 CPNE7 19615732,19615732 0004,0007 HT ANXA2P2 USP53 19615732,19615732 0004,0007 HT CSTA USP53 19615732,19615732 0004,0007 HT CAT USP53 19615732,19615732 0004,0007 HT ARG1 USP53 19615732,19615732 0004,0007 HT AZGP1 USP53 19615732,19615732 0004,0007 HT PPP2R1B USP53 19615732,19615732 0004,0007 HT CASP14 USP53 19615732,19615732 0004,0007 HT HCFC1 USP53 19615732,19615732 0004,0007 HT PSMA6 USP53 19615732,19615732 0004,0007 HT RPL26 USP53 19615732,19615732 0004,0007 HT DSG1 USP53 19615732,19615732 0004,0007 HT TGM3 USP53 19615732,19615732 0004,0007 HT BLMH USP53 19615732,19615732 0004,0007 HT FLNC USP53 19615732,19615732 0004,0007 HT USP53 KRT77 19615732,19615732 0004,0007 HT USP53 RAD50 19615732,19615732 0004,0007 HT USP53 YAE1D1 19615732,19615732 0004,0007 HT USP53 CPNE7 19615732,19615732 0004,0007 HT CRK USP53 27107012,27107012 1356,2215 HT IREB2 USP53 21988832 0397 HT GRB2 USP53 20936779,20936779 0398,0018 HT TRAF2 USP53 16189514,16189514 0398,0018 HT USP53 PVRL2 21988832 0397 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q8CZX0 Q70EK8 Q8CZX0 USP53 0.126 20711500 Q70EK8 O75553 USP53 DAB1 0.183 25416956 Q70EK8 A6NMY6 USP53 ANXA2P2 0.126 19615732 Q70EK8 Q0IIN1 USP53 KRT77 0.126 19615732 Q70EK8 Q12933 USP53 TRAF2 0.454 16189514,unassigned1304 Q7Z794 Q70EK8 KRT77 USP53 0.126 19615732 Q81QG7 Q70EK8 Q81QG7 USP53 0.126 20711500 Q92692 Q70EK8 PVRL2 USP53 0.286 21988832 Q70EK8 P25311 USP53 AZGP1 0.236 19615732 Q70EK8 P05089 USP53 ARG1 0.236 19615732 Q70EK8 P04040 USP53 CAT 0.236 19615732 Q70EK8 P01040 USP53 CSTA 0.236 19615732 Q70EK8 P61254 USP53 RPL26 0.236 19615732 Q70EK8 P30154 USP53 PPP2R1B 0.236 19615732 Q70EK8 P31944 USP53 CASP14 0.236 19615732 Q70EK8 P60900 USP53 PSMA6 0.236 19615732 Q70EK8 P46109 USP53 CRKL 0.236 unassigned1304 Q70EK8 P51610 USP53 HCFC1 0.236 19615732 Q9UBL6 Q70EK8 CPNE7 USP53 0.236 19615732 Q70EK8 Q13867 USP53 BLMH 0.236 19615732 Q70EK8 Q02413 USP53 DSG1 0.236 19615732 Q70EK8 Q08188 USP53 TGM3 0.236 19615732 Q9NRH1 Q70EK8 YAE1D1 USP53 0.236 19615732 Q70EK8 Q14315 USP53 FLNC 0.236 19615732 Q92878 Q70EK8 RAD50 USP53 0.236 19615732 Q70EK8 P62993 USP53 GRB2 0.286 20936779 Q92466 Q70EK8 DDB2 USP53 0.454 23159851 Q70EK8 P46108 USP53 CRK 0.416 27107012,unassigned1304 Q70EK8 P48200 USP53 IREB2 0.286 21988832

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 ANXA2P2 USP53 MI:0045 experimental interaction detection MI:0461 interaction database DAB1 USP53 MI:0045 experimental interaction detection MI:0461 interaction database CSTA USP53 MI:0045 experimental interaction detection MI:0461 interaction database CAT USP53 MI:0045 experimental interaction detection MI:0461 interaction database ARG1 USP53 MI:0045 experimental interaction detection MI:0461 interaction database UBC USP53 MI:0045 experimental interaction detection MI:0461 interaction database AZGP1 USP53 MI:0045 experimental interaction detection MI:0461 interaction database PPP2R1B USP53 MI:0045 experimental interaction detection MI:0461 interaction database CASP14 USP53 MI:0045 experimental interaction detection MI:0461 interaction database CRK USP53 MI:0045 experimental interaction detection MI:0461 interaction database IREB2 USP53 MI:0045 experimental interaction detection MI:0461 interaction database HCFC1 USP53 MI:0045 experimental interaction detection MI:0461 interaction database PSMA6 USP53 MI:0045 experimental interaction detection MI:0461 interaction database RPL26 USP53 MI:0045 experimental interaction detection MI:0461 interaction database GRB2 USP53 MI:0045 experimental interaction detection MI:0461 interaction database DSG1 USP53 MI:0045 experimental interaction detection MI:0461 interaction database TGM3 USP53 MI:0045 experimental interaction detection MI:0461 interaction database TRAF2 USP53 MI:0045 experimental interaction detection MI:0461 interaction database BLMH USP53 MI:0045 experimental interaction detection MI:0461 interaction database FLNC USP53 MI:0045 experimental interaction detection MI:0461 interaction database USP53 KRT77 MI:0045 experimental interaction detection MI:0461 interaction database USP53 DDB2 MI:0045 experimental interaction detection MI:0461 interaction database USP53 NECTIN2 MI:0045 experimental interaction detection MI:0461 interaction database USP53 RAD50 MI:0045 experimental interaction detection MI:0461 interaction database USP53 YAE1D1 MI:0045 experimental interaction detection MI:0461 interaction database USP53 CPNE7 MI:0045 experimental interaction detection MI:0461 interaction database