Classification:            E3 activity/N-recognin/Other

※ E3 activity/N-recognin/Other family introduction

    In the N-end rule pathway, the N-degrons are recognized by N-recognins, a type of E3 ligases. Since there are various N-degrons including UBR-box, N-domain and the others, different protein domains are employed by organisms to recognize (2). Although there are UBR-box domain and N-domain responsible for recognizing UBR-box and N-domain N-degrons, the understanding of recognization of other N-degrons is still limited. Currently, ZZ domain, which is about 50 residues C6H2-type zinc finger, was proposed to mediates degradation of N-degron substrates. PRT1 of Arabidopsis thaliana, which contains one ZZ domain,had been presented to have a ubiquitin ligase activity that mediates degradation of N-degron substrates However, it has no homology to sequence of mammalian N-recognins. Since it does not belong to UBR-box or N-domain, we classify it and its homologous proteins of other plants into the others (1-2).

1. Stary, S., Yin, X.J., Potuschak, T., Schlogelhofer, P., Nizhynska, V. and Bachmair, A. (2003). PRT1 of Arabidopsis is a ubiquitin protein ligase of the plant N-end rule pathway with specificity for aromatic amino-terminal residues. Plant Physiol, 133, 1360-1366. PMID:14551326

2. Sriram, S.M., Kim, B.Y. and Kwon, Y.T. (2011). The N-end rule pathway: emerging functions and molecular principles of substrate recognition. Nat. Rev. Mol. Cell Biol., 12, 735-747. PMID: 22016057

  Amborella trichopoda (1)  Arabidopsis thaliana (1)  Ashbya gossypii (1)
  Brachypodium distachyon (1)  Brassica oleracea (1)  Brassica rapa (1)
  Ciona intestinalis (1)  Ciona savignyi (1)  Cryptococcus neoformans (1)
  Dasypus novemcinctus (1)  Dipodomys ordii (1)  Echinops telfairi (1)
  Ictidomys tridecemlineatus (1)  Latimeria chalumnae (1)  Oryza sativa (1)
  Ostreococcus lucimarinus (1)  Physcomitrella patens (1)  Procavia capensis (1)
  Puccinia graminis (1)  Puccinia triticina (1)  Selaginella moellendorffii (1)
  Tupaia belangeri (1)