iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Bol-015487

Ensembl Protein ID

Bo4g045940.1

UniProt Accession

A0A0D3BSS8; A0A0D3BSS8_BRAOL

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
Bo4g045940	Bo4g045940.1	Bo4g045940.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	4.60e-116	390.0	18	525

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesi 97
           YDRQ+r+wGe++q++l++a+++l+++g++G+E lKnLvl+G+gsitvvD+++ve+ +l+++F++++++vg sKA++++++l+eln+ v++ ++ee++
   Q: 18   YDRQLRIWGEVGQAALENASICLLNCGPTGSEALKNLVLGGIGSITVVDGSRVEIGDLGNNFMVDKKSVGESKAKTVCAFLQELNDAVRAHFVEENP 114
           ************************************************************************************************* PP
   S: 98   teleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn......... 181
           + l  +++ Ff++f++V++++  ++++ k++r+c++a+v l+ ++++Gl G vr+++k++t+++s++d   d++++++P+++lk +++         
   Q: 115  DSLILTDPsFFSQFTLVIATQLVEDSMVKLDRICREANVMLVFARSYGLTGLVRISVKEHTIIDSKPDhflDDLRLNNPWPELKRFVEtidlktpdp 211
           **************************************************************************************99999999999 PP
   S: 182  aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv... 274
            a+++i+++v++ k+++e+a+ ++   ++++eek+e+kd+vks+++s+de+n++ea+e+++k fa++ i++ i++++++ + ++v +++s+fwv   
   Q: 212  IAHKHIPYVVILVKMADEWAKTHSSHLPSTREEKREFKDLVKSKMVSMDEDNYKEAVEATFKVFAPRGISKeIQEIIDD-RYAEVGSNSSAFWVmva 307
           9**********************************************************************99999999.999************** PP
   S: 275  ........................................................................sfcsnaealq...........vpek 288
                                                                                   sfc+na++l+           ++++
   Q: 308  alkefisnegdgeaplegsmpdmtsstehyinlqkiyhtkaeddclsieqrvkdilakvgrdpssiskqtikSFCKNARKLKvcryrmiedefSNPS 404
           **********************************************************************************888888877775566 PP
   S: 289  vekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattnavvgGl 379
           v++ ++ ++++++     +y+llra++rf++++++ pg+++  + +++a +l t a + +  ++g+ + +l+++l  e+++++++++++++a++gG+
   Q: 405  VTELQKCLASQDYssaigFYILLRAVDRFAATYNKFPGQFDGGDIEEDASRLRTIAPSVI-NEMGCDGyELPEELCNEMCRFGAAELHVVAAFIGGI 500
           6666666666666799*******************************9999999888877.************************************ PP
   S: 380  aaqEviKvvsgkfeplknfflfdae 404
           a+qEviK+++++f+p+  +f+f+++
   Q: 501  ASQEVIKLITKQFVPMLGTFVFNGI 525
           *********************9986 PP

Organism

Brassica oleracea

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MERDEQEPTM SEPTKTKYDR QLRIWGEVGQ AALENASICL LNCGPTGSEA LKNLVLGGIG 60
SITVVDGSRV EIGDLGNNFM VDKKSVGESK AKTVCAFLQE LNDAVRAHFV EENPDSLILT 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGGAAAGGG ACGAGCAAGA ACCAACAATG TCTGAGCCCA CGAAAACCAA GTACGATCGC 60
CAACTCAGGT AACCCCTCAA ACCCTCGACT TCAACGTCCG ATCGCTCCAA GTCTTCATCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0005777--C:peroxisome
GO:0019781--F:NEDD8 activating enzyme activity
GO:0009791--P:post-embryonic development
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aly-006187	Arabidopsis lyrata	83.81	0.00e+00	913.00
IUUC-Ago-007900	Ashbya gossypii	25.61	3.00e-35	142.00
IUUC-Bra-017506	Brassica rapa	96.19	0.00e+00	1053.00
IUUC-Cme-027210	Cyanidioschyzon merolae	26.46	9.00e-16	78.60
IUUC-Dse-031536	Dothistroma septosporum	32.91	1.00e-73	270.00
IUUC-Ggr-040005	Gaeumannomyces graminis	30.99	8.00e-62	231.00
IUUC-Lma-053112	Leptosphaeria maculans	34.59	2.00e-78	286.00
IUUC-Mor-057230	Magnaporthe oryzae	32.31	3.00e-67	248.00
IUUC-Mvi-060221	Microbotryum violaceum	32.63	5.00e-73	268.00
IUUC-Ncr-068910	Neurospora crassa	32.30	4.00e-70	258.00
IUUC-Olu-087625	Ostreococcus lucimarinus	31.08	8.00e-64	238.00
IUUC-Pte-104355	Pyrenophora teres	34.88	2.00e-76	279.00
IUUC-Pyt-104545	Pyrenophora triticirepentis	35.37	1.00e-75	277.00
IUUC-Smo-109296	Selaginella moellendorffii	52.99	1.00e-163	569.00
IUUC-Tre-122103	Trichoderma reesei	32.84	6.00e-70	258.00
IUUC-Tme-127097	Tuber melanosporum	35.66	4.00e-83	301.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence