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Basic Information Integrated Annotations

Tag Content
iUUCD ID IUUC-Ath-006623
UUCD1 version UUC-ArT-01618
Ensembl Protein ID AT5G19180.1
UniProt Accession O65041; Q0WWG4; Q94A29; UBA3_ARATH
Genbank Protein ID AAC27035.1; AED92667.1; AAK91442.1; AAM52235.1; BAE98534.1
Protein Name NEDD8-activating enzyme E1 catalytic subunit; RUB-activating enzyme; Ubiquitin-activating enzyme E1-like protein
Genbank Nucleotide ID AF051135; AC069326; AY050426; AY120692; AK226388
Gene Name ECR1; At5g19180; T24G5_80
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
AT5G19180 AT5G19180.1 AT5G19180.1
AT5G19180 AT5G19180.2 AT5G19180.2
Annotation
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
Post-translational Modifications (PTMs)
UniProt
Protein Expression/Proteomics
GPMDB
Status Reviewed
Details
Family Domain References (PMIDs)
E1 E1_ThiF 12215511
Classification
Family E-value Score Start End
E1 1.20e-89 302.3 45 234
Active Site
Position(s) Description Evidence
215 Glycyl thioester intermediate N/A
Domain Profile

   E1

   S: 17   ksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfkkfdvVv 114
    + ++l++GagglGcEllK L+l+G+++++v+Dmd++ev+nlnrqFLfr edvgk+KAevaa+++ e+++ v++++h ++i+++e e F+++f++++
   Q: 45 DYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIE--FYNDFNIIA 140
    6789********************************************************************************888..********* PP
   S: 115 laldnrearrkvnrlclna.............rvpliesgtlGllGqvrviikgltecyscsd..dppqktiPfctlketpnaaehtiewavlf 193
    l+ld++ear+++n + + +p++++gt+G++G++rvi++g+t c +c+ ppq+++P+ctl+etp++a h+ie+a+l+
   Q: 141 LGLDSIEARKYINGVACGFleyneddtpkretIKPMVDGGTEGFKGHARVILPGVTPCFECTIylFPPQVKFPLCTLAETPRNAAHCIEYAHLI 234
    ******************9************************************************************************544 PP
   

Organism Arabidopsis thaliana
Functional Description
(View)

Functional Description



     Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1 (By similarity).
Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1 (By similarity).
Protein Sequence
(Fasta)
MADLDVPPQV PQSKTRDLDK LLLRHGNLVD PGFVPGPGLR DDIRDYVRIL VIGAGGLGCE 60
LLKDLALSGF RNLEVIDMDR IEVTNLNRQF LFRIEDVGKP KAEVAAKRVM ERVSGVEIVP 120
It may take some time, please wait.

Protein Fasta Sequence



>IUUC-Ath-006623|E1,E1_ThiF|Arabidopsis thaliana
Please wait for a moment...
Nucleotide Sequence
(Fasta)
AGGGTCGGCT CAAAGAGAGA AGCCAATACA AGTTTCGGAG CTCTGTGGCA GTGTATCGGC 60
TTTGCGTCTT TTGAGCTCTC TCCGCCGTGA TCAATCTCCG TGAATCGCCG CCACTCTCTC 120
It may take some time, please wait.

Nucleotide Fasta Sequence



>IUUC-Ath-006623|E1,E1_ThiF|Arabidopsis thaliana
Please wait for a moment...
Sequence Source Ensembl
Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR014929--E2_binding
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR030468--Uba3
IPR033127--UBQ-activ_enz_E1_Cys_AS

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF08825--E2_bind
PF00899--ThiF

SMART

SM01181--E2_bind

Gene Ontology

GO:0005634--C:nucleus
GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0019781--F:NEDD8 activating enzyme activity
GO:0046982--F:protein heterodimerization activity
GO:0045116--P:protein neddylation

KEGG ath:AT5G19180
Orthology
iUUCD ID Species Identity E-value Score
IUUC-Ata-001124 Aegilops tauschii 73.26 8.00e-156 542.00
IUUC-Aml-001578 Ailuropoda melanoleuca 47.39 2.00e-104 371.00
IUUC-Atr-002591 Amborella trichopoda 74.32 2.00e-179 620.00
IUUC-Apl-003671 Anas platyrhynchos 47.89 1.00e-105 375.00
IUUC-Aca-004749 Anolis carolinensis 47.88 6.00e-107 380.00
IUUC-Aly-005747 Arabidopsis lyrata 96.92 0.00e+00 870.00
IUUC-Ago-007934 Ashbya gossypii 40.25 8.00e-61 226.00
IUUC-Acl-008047 Aspergillus clavatus 47.94 2.00e-109 388.00
IUUC-Afl-008615 Aspergillus flavus 42.05 1.00e-66 246.00
IUUC-Afu-008996 Aspergillus fumigatus 48.97 2.00e-99 355.00
IUUC-Ani-009281 Aspergillus nidulans 50.46 1.00e-73 269.00
IUUC-Ang-009539 Aspergillus niger 48.37 4.00e-97 347.00
IUUC-Aor-010151 Aspergillus oryzae 46.12 2.00e-91 328.00
IUUC-Ate-010502 Aspergillus terreus 50.13 1.00e-98 352.00
IUUC-Ame-011837 Astyanax mexicanus 48.74 2.00e-96 345.00
IUUC-Bgr-012068 Blumeria graminis 49.45 1.00e-93 335.00
IUUC-Bta-013003 Bos taurus 47.89 1.00e-105 375.00
IUUC-Bci-013983 Botrytis cinerea 49.38 7.00e-98 350.00
IUUC-Bdi-014429 Brachypodium distachyon 72.15 5.00e-169 586.00
IUUC-Bol-015813 Brassica oleracea 87.89 0.00e+00 752.00
IUUC-Bra-018023 Brassica rapa 87.33 0.00e+00 736.00
IUUC-Cel-018684 Caenorhabditis elegans 48.50 4.00e-101 360.00
IUUC-Cja-019200 Callithrix jacchus 47.90 4.00e-103 367.00
IUUC-Cfa-021038 Canis familiaris 47.89 2.00e-105 375.00
IUUC-Cre-022778 Chlamydomonas reinhardtii 58.72 3.00e-136 477.00
IUUC-Csa-024079 Chlorocebus sabaeus 47.64 2.00e-105 375.00
IUUC-Cho-025052 Choloepus hoffmanni 41.94 7.00e-87 313.00
IUUC-Cin-025113 Ciona intestinalis 48.75 7.00e-100 356.00
IUUC-Csv-026231 Ciona savignyi 48.27 4.00e-105 374.00
IUUC-Cgl-026535 Colletotrichum gloeosporioides 46.82 1.00e-93 336.00
IUUC-Cne-026850 Cryptococcus neoformans 48.47 4.00e-94 337.00
IUUC-Cme-027275 Cyanidioschyzon merolae 37.47 1.00e-74 273.00
IUUC-Dre-028751 Danio rerio 47.64 1.00e-95 342.00
IUUC-Dno-029571 Dasypus novemcinctus 47.89 1.00e-105 375.00
IUUC-Dor-030395 Dipodomys ordii 48.50 1.00e-105 376.00
IUUC-Dse-031329 Dothistroma septosporum 44.29 8.00e-91 326.00
IUUC-Dme-031776 Drosophila melanogaster 48.54 5.00e-99 353.00
IUUC-Ete-032894 Echinops telfairi 49.70 9.00e-39 153.00
IUUC-Eca-034389 Equus caballus 47.89 2.00e-105 375.00
IUUC-Eeu-035152 Erinaceus europaeus 45.21 7.00e-82 296.00
IUUC-Fca-036032 Felis catus 47.89 3.00e-105 374.00
IUUC-Fal-037506 Ficedula albicollis 48.39 5.00e-108 384.00
IUUC-Fox-037871 Fusarium oxysporum 51.16 2.00e-88 318.00
IUUC-Fso-038350 Fusarium solani 47.88 1.00e-95 342.00
IUUC-Gmo-039246 Gadus morhua 48.87 7.00e-96 343.00
IUUC-Ggr-039859 Gaeumannomyces graminis 48.29 3.00e-98 351.00
IUUC-Gga-040843 Gallus gallus 47.39 2.00e-101 362.00
IUUC-Gac-042601 Gasterosteus aculeatus 47.64 1.00e-96 345.00
IUUC-Gma-042744 Glycine max 78.79 0.00e+00 641.00
IUUC-Ggo-044788 Gorilla gorilla 47.77 2.00e-104 371.00
IUUC-Hsa-045849 Homo sapiens 47.89 1.00e-105 376.00
IUUC-Hvu-047819 Hordeum vulgare 78.26 2.00e-143 501.00
IUUC-Itr-047863 Ictidomys tridecemlineatus 47.64 2.00e-105 375.00
IUUC-Kpa-049393 Komagataella pastoris 52.36 7.00e-84 303.00
IUUC-Lch-049471 Latimeria chalumnae 47.39 1.00e-97 349.00
IUUC-Lpe-051297 Leersia perrieri 68.97 8.00e-176 608.00
IUUC-Loc-052910 Lepisosteus oculatus 49.49 1.00e-97 349.00
IUUC-Lma-053181 Leptosphaeria maculans 48.06 3.00e-101 361.00
IUUC-Laf-054314 Loxodonta africana 47.06 3.00e-103 367.00
IUUC-Mcc-054870 Macaca mulatta 47.89 9.00e-106 376.00
IUUC-Meu-056574 Macropus eugenii 40.14 2.00e-44 172.00
IUUC-Mor-056919 Magnaporthe oryzae 47.72 4.00e-93 334.00
IUUC-Mpo-057614 Magnaporthe poae 46.23 2.00e-91 328.00
IUUC-Mtr-058110 Medicago truncatula 78.09 0.00e+00 637.00
IUUC-Mla-058949 Melampsora laricipopulina 44.33 7.00e-93 333.00
IUUC-Mga-059455 Meleagris gallopavo 47.89 6.00e-106 377.00
IUUC-Mvi-060270 Microbotryum violaceum 45.43 2.00e-95 342.00
IUUC-Mmr-061155 Microcebus murinus 49.37 5.00e-105 374.00
IUUC-Mdo-062037 Monodelphis domestica 48.61 9.00e-105 373.00
IUUC-Mmu-063670 Mus musculus 48.39 5.00e-106 377.00
IUUC-Mac-064711 Musa acuminata 71.23 8.00e-165 572.00
IUUC-Mpu-066734 Mustela putorius furo 47.90 2.00e-105 375.00
IUUC-Mlu-067068 Myotis lucifugus 48.63 5.00e-106 377.00
IUUC-Nfi-068349 Neosartorya fischeri 48.97 2.00e-99 355.00
IUUC-Ncr-068626 Neurospora crassa 47.90 1.00e-94 338.00
IUUC-Nle-069366 Nomascus leucogenys 51.64 2.00e-99 355.00
IUUC-Ont-072196 Oreochromis niloticus 48.86 1.00e-94 339.00
IUUC-Oan-073511 Ornithorhynchus anatinus 46.65 5.00e-103 367.00
IUUC-Ocu-073773 Oryctolagus cuniculus 48.14 9.00e-106 376.00
IUUC-Oba-075726 Oryza barthii 63.97 1.00e-158 551.00
IUUC-Obr-076286 Oryza brachyantha 69.86 3.00e-174 603.00
IUUC-Ogu-078473 Oryza glumaepatula 64.25 9.00e-159 552.00
IUUC-Oin-079393 Oryza indica 69.63 1.00e-174 605.00
IUUC-Olo-080446 Oryza longistaminata 65.63 9.00e-163 565.00
IUUC-Ome-081737 Oryza meridionalis 66.89 3.00e-162 563.00
IUUC-Oni-082853 Oryza nivara 63.97 1.00e-158 551.00
IUUC-Opu-083590 Oryza punctata 67.63 3.00e-172 597.00
IUUC-Oru-084184 Oryza rufipogon 63.97 1.00e-158 551.00
IUUC-Osa-085402 Oryza sativa 69.63 1.00e-174 605.00
IUUC-Ola-086671 Oryzias latipes 50.13 4.00e-89 320.00
IUUC-Olu-087738 Ostreococcus lucimarinus 57.18 4.00e-120 423.00
IUUC-Oga-088101 Otolemur garnettii 47.89 1.00e-105 375.00
IUUC-Oar-090293 Ovis aries 47.30 2.00e-103 369.00
IUUC-Ptr-090503 Pan troglodytes 47.89 1.00e-105 376.00
IUUC-Pan-092477 Papio anubis 45.91 1.00e-100 359.00
IUUC-Psi-093442 Pelodiscus sinensis 48.88 4.00e-108 384.00
IUUC-Pma-094247 Petromyzon marinus 48.76 8.00e-96 343.00
IUUC-Pno-094780 Phaeosphaeria nodorum 46.95 6.00e-101 360.00
IUUC-Ppa-095961 Physcomitrella patens 59.65 8.00e-162 562.00
IUUC-Pfo-096846 Poecilia formosa 47.29 2.00e-97 348.00
IUUC-Pab-098060 Pongo abelii 47.64 4.00e-97 347.00
IUUC-Pop-099914 Populus trichocarpa 77.62 0.00e+00 654.00
IUUC-Pca-100500 Procavia capensis 44.51 5.00e-73 267.00
IUUC-Ppe-102008 Prunus persica 77.93 0.00e+00 636.00
IUUC-Pva-103095 Pteropus vampyrus 55.05 6.00e-59 220.00
IUUC-Pgr-103666 Puccinia graminis 46.53 2.00e-98 352.00
IUUC-Ptt-103901 Puccinia triticina 46.80 9.00e-98 350.00
IUUC-Pte-104198 Pyrenophora teres 47.29 1.00e-99 355.00
IUUC-Pyt-104792 Pyrenophora triticirepentis 46.80 1.00e-96 345.00
IUUC-Rno-105446 Rattus norvegicus 48.14 8.00e-106 376.00
IUUC-Sce-106355 Saccharomyces cerevisiae 39.74 3.00e-59 221.00
IUUC-Sha-106664 Sarcophilus harrisii 48.61 5.00e-105 374.00
IUUC-Sja-107982 Schizosaccharomyces japonicus 41.36 2.00e-85 308.00
IUUC-Spo-108345 Schizosaccharomyces pombe 41.56 3.00e-87 314.00
IUUC-Ssl-108540 Sclerotinia sclerotiorum 48.76 9.00e-83 300.00
IUUC-Smo-109098 Selaginella moellendorffii 58.67 5.00e-156 543.00
IUUC-Sit-109724 Setaria italica 68.97 2.00e-164 571.00
IUUC-Sly-110916 Solanum lycopersicum 76.46 0.00e+00 651.00
IUUC-Stu-112236 Solanum tuberosum 76.23 0.00e+00 652.00
IUUC-Sar-113044 Sorex araneus 50.00 4.00e-102 364.00
IUUC-Sbi-114276 Sorghum bicolor 68.36 2.00e-165 574.00
IUUC-Sre-114963 Sporisorium reilianum 47.72 5.00e-97 347.00
IUUC-Ssc-116301 Sus scrofa 47.06 6.00e-103 367.00
IUUC-Tgu-117265 Taeniopygia guttata 48.39 8.00e-108 383.00
IUUC-Tru-117526 Takifugu rubripes 49.75 8.00e-96 343.00
IUUC-Tsy-119483 Tarsius syrichta 49.22 3.00e-103 368.00
IUUC-Tni-120363 Tetraodon nigroviridis 50.63 2.00e-99 355.00
IUUC-Tca-121731 Theobroma cacao 78.83 0.00e+00 662.00
IUUC-Tre-122221 Trichoderma reesei 49.71 3.00e-87 314.00
IUUC-Tvi-122352 Trichoderma virens 48.30 3.00e-95 341.00
IUUC-Tae-123864 Triticum aestivum 73.06 1.00e-171 595.00
IUUC-Tur-126070 Triticum urartu 72.91 3.00e-147 514.00
IUUC-Tme-127199 Tuber melanosporum 51.12 2.00e-97 348.00
IUUC-Tbe-128076 Tupaia belangeri 47.89 1.00e-105 375.00
IUUC-Ttr-128695 Tursiops truncatus 53.47 4.00e-40 158.00
IUUC-Uma-129638 Ustilago maydis 46.67 5.00e-95 340.00
IUUC-Vda-129963 Verticillium dahliae 48.69 3.00e-87 314.00
IUUC-Vpa-130637 Vicugna pacos 41.69 3.00e-83 301.00
IUUC-Vvi-131613 Vitis vinifera 79.02 0.00e+00 648.00
IUUC-Xtr-132924 Xenopus tropicalis 45.89 4.00e-95 340.00
IUUC-Xma-134209 Xiphophorus maculatus 47.52 1.00e-97 349.00
IUUC-Yli-134365 Yarrowia lipolytica 47.79 2.00e-95 342.00
IUUC-Zma-135941 Zea mays 62.15 2.00e-157 548.00
Created Date 25-Jun-2017

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