iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Ath-006623

UUCD1 version

UUC-ArT-01618

Ensembl Protein ID

AT5G19180.1

UniProt Accession

O65041; Q0WWG4; Q94A29; UBA3_ARATH

Genbank Protein ID

AAC27035.1; AED92667.1; AAK91442.1; AAM52235.1; BAE98534.1

Protein Name

NEDD8-activating enzyme E1 catalytic subunit; RUB-activating enzyme; Ubiquitin-activating enzyme E1-like protein

Genbank Nucleotide ID

AF051135; AC069326; AY050426; AY120692; AK226388

Gene Name

ECR1; At5g19180; T24G5_80

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
AT5G19180	AT5G19180.1	AT5G19180.1
AT5G19180	AT5G19180.2	AT5G19180.2

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
Post-translational Modifications (PTMs)
UniProt
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	12215511

Classification

Family	E-value	Score	Start	End
E1	1.20e-89	302.3	45	234

Active Site

Position(s)	Description	Evidence
215	Glycyl thioester intermediate	N/A

Domain Profile

   S: 17   ksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfkkfdvVv 114
           +  ++l++GagglGcEllK L+l+G+++++v+Dmd++ev+nlnrqFLfr edvgk+KAevaa+++ e+++ v++++h ++i+++e e  F+++f++++
   Q: 45   DYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIE--FYNDFNIIA 140
           6789********************************************************************************888..********* PP
   S: 115  laldnrearrkvnrlclna.............rvpliesgtlGllGqvrviikgltecyscsd..dppqktiPfctlketpnaaehtiewavlf 193
           l+ld++ear+++n + +                +p++++gt+G++G++rvi++g+t c +c+    ppq+++P+ctl+etp++a h+ie+a+l+
   Q: 141  LGLDSIEARKYINGVACGFleyneddtpkretIKPMVDGGTEGFKGHARVILPGVTPCFECTIylFPPQVKFPLCTLAETPRNAAHCIEYAHLI 234
           ******************9************************************************************************544 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1 (By similarity).

Protein Sequence
(Fasta)

MADLDVPPQV PQSKTRDLDK LLLRHGNLVD PGFVPGPGLR DDIRDYVRIL VIGAGGLGCE 60
LLKDLALSGF RNLEVIDMDR IEVTNLNRQF LFRIEDVGKP KAEVAAKRVM ERVSGVEIVP 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AGGGTCGGCT CAAAGAGAGA AGCCAATACA AGTTTCGGAG CTCTGTGGCA GTGTATCGGC 60
TTTGCGTCTT TTGAGCTCTC TCCGCCGTGA TCAATCTCCG TGAATCGCCG CCACTCTCTC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR014929--E2_binding
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR030468--Uba3
IPR033127--UBQ-activ_enz_E1_Cys_AS

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF08825--E2_bind
PF00899--ThiF

SMART

SM01181--E2_bind

Gene Ontology

GO:0005634--C:nucleus
GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0019781--F:NEDD8 activating enzyme activity
GO:0046982--F:protein heterodimerization activity
GO:0045116--P:protein neddylation

KEGG

ath:AT5G19180

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001124	Aegilops tauschii	73.26	8.00e-156	542.00
IUUC-Aml-001578	Ailuropoda melanoleuca	47.39	2.00e-104	371.00
IUUC-Atr-002591	Amborella trichopoda	74.32	2.00e-179	620.00
IUUC-Apl-003671	Anas platyrhynchos	47.89	1.00e-105	375.00
IUUC-Aca-004749	Anolis carolinensis	47.88	6.00e-107	380.00
IUUC-Aly-005747	Arabidopsis lyrata	96.92	0.00e+00	870.00
IUUC-Ago-007934	Ashbya gossypii	40.25	8.00e-61	226.00
IUUC-Acl-008047	Aspergillus clavatus	47.94	2.00e-109	388.00
IUUC-Afl-008615	Aspergillus flavus	42.05	1.00e-66	246.00
IUUC-Afu-008996	Aspergillus fumigatus	48.97	2.00e-99	355.00
IUUC-Ani-009281	Aspergillus nidulans	50.46	1.00e-73	269.00
IUUC-Ang-009539	Aspergillus niger	48.37	4.00e-97	347.00
IUUC-Aor-010151	Aspergillus oryzae	46.12	2.00e-91	328.00
IUUC-Ate-010502	Aspergillus terreus	50.13	1.00e-98	352.00
IUUC-Ame-011837	Astyanax mexicanus	48.74	2.00e-96	345.00
IUUC-Bgr-012068	Blumeria graminis	49.45	1.00e-93	335.00
IUUC-Bta-013003	Bos taurus	47.89	1.00e-105	375.00
IUUC-Bci-013983	Botrytis cinerea	49.38	7.00e-98	350.00
IUUC-Bdi-014429	Brachypodium distachyon	72.15	5.00e-169	586.00
IUUC-Bol-015813	Brassica oleracea	87.89	0.00e+00	752.00
IUUC-Bra-018023	Brassica rapa	87.33	0.00e+00	736.00
IUUC-Cel-018684	Caenorhabditis elegans	48.50	4.00e-101	360.00
IUUC-Cja-019200	Callithrix jacchus	47.90	4.00e-103	367.00
IUUC-Cfa-021038	Canis familiaris	47.89	2.00e-105	375.00
IUUC-Cre-022778	Chlamydomonas reinhardtii	58.72	3.00e-136	477.00
IUUC-Csa-024079	Chlorocebus sabaeus	47.64	2.00e-105	375.00
IUUC-Cho-025052	Choloepus hoffmanni	41.94	7.00e-87	313.00
IUUC-Cin-025113	Ciona intestinalis	48.75	7.00e-100	356.00
IUUC-Csv-026231	Ciona savignyi	48.27	4.00e-105	374.00
IUUC-Cgl-026535	Colletotrichum gloeosporioides	46.82	1.00e-93	336.00
IUUC-Cne-026850	Cryptococcus neoformans	48.47	4.00e-94	337.00
IUUC-Cme-027275	Cyanidioschyzon merolae	37.47	1.00e-74	273.00
IUUC-Dre-028751	Danio rerio	47.64	1.00e-95	342.00
IUUC-Dno-029571	Dasypus novemcinctus	47.89	1.00e-105	375.00
IUUC-Dor-030395	Dipodomys ordii	48.50	1.00e-105	376.00
IUUC-Dse-031329	Dothistroma septosporum	44.29	8.00e-91	326.00
IUUC-Dme-031776	Drosophila melanogaster	48.54	5.00e-99	353.00
IUUC-Ete-032894	Echinops telfairi	49.70	9.00e-39	153.00
IUUC-Eca-034389	Equus caballus	47.89	2.00e-105	375.00
IUUC-Eeu-035152	Erinaceus europaeus	45.21	7.00e-82	296.00
IUUC-Fca-036032	Felis catus	47.89	3.00e-105	374.00
IUUC-Fal-037506	Ficedula albicollis	48.39	5.00e-108	384.00
IUUC-Fox-037871	Fusarium oxysporum	51.16	2.00e-88	318.00
IUUC-Fso-038350	Fusarium solani	47.88	1.00e-95	342.00
IUUC-Gmo-039246	Gadus morhua	48.87	7.00e-96	343.00
IUUC-Ggr-039859	Gaeumannomyces graminis	48.29	3.00e-98	351.00
IUUC-Gga-040843	Gallus gallus	47.39	2.00e-101	362.00
IUUC-Gac-042601	Gasterosteus aculeatus	47.64	1.00e-96	345.00
IUUC-Gma-042744	Glycine max	78.79	0.00e+00	641.00
IUUC-Ggo-044788	Gorilla gorilla	47.77	2.00e-104	371.00
IUUC-Hsa-045849	Homo sapiens	47.89	1.00e-105	376.00
IUUC-Hvu-047819	Hordeum vulgare	78.26	2.00e-143	501.00
IUUC-Itr-047863	Ictidomys tridecemlineatus	47.64	2.00e-105	375.00
IUUC-Kpa-049393	Komagataella pastoris	52.36	7.00e-84	303.00
IUUC-Lch-049471	Latimeria chalumnae	47.39	1.00e-97	349.00
IUUC-Lpe-051297	Leersia perrieri	68.97	8.00e-176	608.00
IUUC-Loc-052910	Lepisosteus oculatus	49.49	1.00e-97	349.00
IUUC-Lma-053181	Leptosphaeria maculans	48.06	3.00e-101	361.00
IUUC-Laf-054314	Loxodonta africana	47.06	3.00e-103	367.00
IUUC-Mcc-054870	Macaca mulatta	47.89	9.00e-106	376.00
IUUC-Meu-056574	Macropus eugenii	40.14	2.00e-44	172.00
IUUC-Mor-056919	Magnaporthe oryzae	47.72	4.00e-93	334.00
IUUC-Mpo-057614	Magnaporthe poae	46.23	2.00e-91	328.00
IUUC-Mtr-058110	Medicago truncatula	78.09	0.00e+00	637.00
IUUC-Mla-058949	Melampsora laricipopulina	44.33	7.00e-93	333.00
IUUC-Mga-059455	Meleagris gallopavo	47.89	6.00e-106	377.00
IUUC-Mvi-060270	Microbotryum violaceum	45.43	2.00e-95	342.00
IUUC-Mmr-061155	Microcebus murinus	49.37	5.00e-105	374.00
IUUC-Mdo-062037	Monodelphis domestica	48.61	9.00e-105	373.00
IUUC-Mmu-063670	Mus musculus	48.39	5.00e-106	377.00
IUUC-Mac-064711	Musa acuminata	71.23	8.00e-165	572.00
IUUC-Mpu-066734	Mustela putorius furo	47.90	2.00e-105	375.00
IUUC-Mlu-067068	Myotis lucifugus	48.63	5.00e-106	377.00
IUUC-Nfi-068349	Neosartorya fischeri	48.97	2.00e-99	355.00
IUUC-Ncr-068626	Neurospora crassa	47.90	1.00e-94	338.00
IUUC-Nle-069366	Nomascus leucogenys	51.64	2.00e-99	355.00
IUUC-Ont-072196	Oreochromis niloticus	48.86	1.00e-94	339.00
IUUC-Oan-073511	Ornithorhynchus anatinus	46.65	5.00e-103	367.00
IUUC-Ocu-073773	Oryctolagus cuniculus	48.14	9.00e-106	376.00
IUUC-Oba-075726	Oryza barthii	63.97	1.00e-158	551.00
IUUC-Obr-076286	Oryza brachyantha	69.86	3.00e-174	603.00
IUUC-Ogu-078473	Oryza glumaepatula	64.25	9.00e-159	552.00
IUUC-Oin-079393	Oryza indica	69.63	1.00e-174	605.00
IUUC-Olo-080446	Oryza longistaminata	65.63	9.00e-163	565.00
IUUC-Ome-081737	Oryza meridionalis	66.89	3.00e-162	563.00
IUUC-Oni-082853	Oryza nivara	63.97	1.00e-158	551.00
IUUC-Opu-083590	Oryza punctata	67.63	3.00e-172	597.00
IUUC-Oru-084184	Oryza rufipogon	63.97	1.00e-158	551.00
IUUC-Osa-085402	Oryza sativa	69.63	1.00e-174	605.00
IUUC-Ola-086671	Oryzias latipes	50.13	4.00e-89	320.00
IUUC-Olu-087738	Ostreococcus lucimarinus	57.18	4.00e-120	423.00
IUUC-Oga-088101	Otolemur garnettii	47.89	1.00e-105	375.00
IUUC-Oar-090293	Ovis aries	47.30	2.00e-103	369.00
IUUC-Ptr-090503	Pan troglodytes	47.89	1.00e-105	376.00
IUUC-Pan-092477	Papio anubis	45.91	1.00e-100	359.00
IUUC-Psi-093442	Pelodiscus sinensis	48.88	4.00e-108	384.00
IUUC-Pma-094247	Petromyzon marinus	48.76	8.00e-96	343.00
IUUC-Pno-094780	Phaeosphaeria nodorum	46.95	6.00e-101	360.00
IUUC-Ppa-095961	Physcomitrella patens	59.65	8.00e-162	562.00
IUUC-Pfo-096846	Poecilia formosa	47.29	2.00e-97	348.00
IUUC-Pab-098060	Pongo abelii	47.64	4.00e-97	347.00
IUUC-Pop-099914	Populus trichocarpa	77.62	0.00e+00	654.00
IUUC-Pca-100500	Procavia capensis	44.51	5.00e-73	267.00
IUUC-Ppe-102008	Prunus persica	77.93	0.00e+00	636.00
IUUC-Pva-103095	Pteropus vampyrus	55.05	6.00e-59	220.00
IUUC-Pgr-103666	Puccinia graminis	46.53	2.00e-98	352.00
IUUC-Ptt-103901	Puccinia triticina	46.80	9.00e-98	350.00
IUUC-Pte-104198	Pyrenophora teres	47.29	1.00e-99	355.00
IUUC-Pyt-104792	Pyrenophora triticirepentis	46.80	1.00e-96	345.00
IUUC-Rno-105446	Rattus norvegicus	48.14	8.00e-106	376.00
IUUC-Sce-106355	Saccharomyces cerevisiae	39.74	3.00e-59	221.00
IUUC-Sha-106664	Sarcophilus harrisii	48.61	5.00e-105	374.00
IUUC-Sja-107982	Schizosaccharomyces japonicus	41.36	2.00e-85	308.00
IUUC-Spo-108345	Schizosaccharomyces pombe	41.56	3.00e-87	314.00
IUUC-Ssl-108540	Sclerotinia sclerotiorum	48.76	9.00e-83	300.00
IUUC-Smo-109098	Selaginella moellendorffii	58.67	5.00e-156	543.00
IUUC-Sit-109724	Setaria italica	68.97	2.00e-164	571.00
IUUC-Sly-110916	Solanum lycopersicum	76.46	0.00e+00	651.00
IUUC-Stu-112236	Solanum tuberosum	76.23	0.00e+00	652.00
IUUC-Sar-113044	Sorex araneus	50.00	4.00e-102	364.00
IUUC-Sbi-114276	Sorghum bicolor	68.36	2.00e-165	574.00
IUUC-Sre-114963	Sporisorium reilianum	47.72	5.00e-97	347.00
IUUC-Ssc-116301	Sus scrofa	47.06	6.00e-103	367.00
IUUC-Tgu-117265	Taeniopygia guttata	48.39	8.00e-108	383.00
IUUC-Tru-117526	Takifugu rubripes	49.75	8.00e-96	343.00
IUUC-Tsy-119483	Tarsius syrichta	49.22	3.00e-103	368.00
IUUC-Tni-120363	Tetraodon nigroviridis	50.63	2.00e-99	355.00
IUUC-Tca-121731	Theobroma cacao	78.83	0.00e+00	662.00
IUUC-Tre-122221	Trichoderma reesei	49.71	3.00e-87	314.00
IUUC-Tvi-122352	Trichoderma virens	48.30	3.00e-95	341.00
IUUC-Tae-123864	Triticum aestivum	73.06	1.00e-171	595.00
IUUC-Tur-126070	Triticum urartu	72.91	3.00e-147	514.00
IUUC-Tme-127199	Tuber melanosporum	51.12	2.00e-97	348.00
IUUC-Tbe-128076	Tupaia belangeri	47.89	1.00e-105	375.00
IUUC-Ttr-128695	Tursiops truncatus	53.47	4.00e-40	158.00
IUUC-Uma-129638	Ustilago maydis	46.67	5.00e-95	340.00
IUUC-Vda-129963	Verticillium dahliae	48.69	3.00e-87	314.00
IUUC-Vpa-130637	Vicugna pacos	41.69	3.00e-83	301.00
IUUC-Vvi-131613	Vitis vinifera	79.02	0.00e+00	648.00
IUUC-Xtr-132924	Xenopus tropicalis	45.89	4.00e-95	340.00
IUUC-Xma-134209	Xiphophorus maculatus	47.52	1.00e-97	349.00
IUUC-Yli-134365	Yarrowia lipolytica	47.79	2.00e-95	342.00
IUUC-Zma-135941	Zea mays	62.15	2.00e-157	548.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence