iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Bol-016401

Ensembl Protein ID

Bo8g004450.1

UniProt Accession

A0A0D3DIY9; A0A0D3DIY9_BRAOL

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
Bo8g004450	Bo8g004450.1	Bo8g004450.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	5.40e-124	416.1	6	512

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesi 97
           YDRQ+r+wGe++q++l++a+++l+++g++G+E lKnLvl+Gvgsit+vD+++ve+ +l+++F++++++vg+sKA++++++l+eln++v+++++ees+
   Q: 6    YDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITIVDGSKVELGDLGNNFMVDERSVGQSKAKSTCAFLQELNDSVKAKFVEESP 102
           ************************************************************************************************* PP
   S: 98   teleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn......... 181
           ++l ++ + Ff++f++V++++  ++++ k++r+c++a+v+ + ++++Gl G vrv++k++t+++s++d   d++++++P+++l ++++         
   Q: 103  DALIMTSPsFFSQFTLVIATQLVEDSMVKLDRICREANVKVVFVRSYGLAGLVRVSVKEHTIIDSKPDhflDDLRLNNPWPELYSFVEtidlnvsdh 199
           **************************************************************************************98899999999 PP
   S: 182  aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv... 274
            a+++i+++v++ k+++e++++++++ ++++eek+e+kd vks++ s+de+n++eaie+a+k fa++ i+s i+q+ ++ +c++ ++++s+fwv   
   Q: 200  IAHKHIPYVVILVKMADEWTKSHSGNLPSTREEKKEFKDFVKSKMISMDEDNYKEAIEAAFKVFAPRGISSeIQQICND-TCAEPSSNSSDFWVmva 295
           *******************************************************************************.99999************ PP
   S: 275  ........................................................................sfcsnaealq...........vpek 288
                                                                                   sfc+na++l+           ++++
   Q: 296  alkefvsnegdgeaplegsipdmtsstehyinlqkiylakaeadflvmekrvknilkrigrdpssiskptikSFCKNARKLKvcryrmvedefSNPS 392
           **********************************************************************************888888877777799 PP
   S: 289  vekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattnavvgGl 379
           v++ ++ ++++++     +y+llra++rf +++++ pg+++  +d+d + +l+t+a++l   +lg+++ +l+ddli e+++++++++++++a+vgG+
   Q: 393  VTEIQKCLADEDYsgamgFYVLLRAVDRFTANYNKFPGQFDGGMDED-ISRLKTTALSLL-ADLGCNGsVLPDDLINEMCRFGASELHVVAAFVGGI 487
           99999999999999*********************************.**********99.9*********************************** PP
   S: 380  aaqEviKvvsgkfeplknfflfdae 404
           a+qEviK+v+++f+p+  +++f+++
   Q: 488  ASQEVIKLVTKQFVPMLGTYIFNGI 512
           ****************999999986 PP

Organism

Brassica oleracea

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MSKTKYDRQL RIWGEVGQAA LEEASICLLN CGPTGSEALK NLVLGGVGSI TIVDGSKVEL 60
GDLGNNFMVD ERSVGQSKAK STCAFLQELN DSVKAKFVEE SPDALIMTSP SFFSQFTLVI 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGTCTAAAA CCAAGTACGA TCGGCAGCTA AGGTATCTAG ATTTACTTCT CTCTCTCTCT 60
CTCTCTCTCT CTCTCTGTAT AAACTCTATA GGTCGTCGTT TAGGGAGTAG AAGTAATCAA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Acl-008333	Aspergillus clavatus	32.60	2.00e-71	263.00
IUUC-Afl-008529	Aspergillus flavus	33.51	9.00e-80	290.00
IUUC-Afu-009071	Aspergillus fumigatus	32.25	5.00e-72	265.00
IUUC-Ani-009443	Aspergillus nidulans	32.29	6.00e-69	254.00
IUUC-Ang-009669	Aspergillus niger	32.12	4.00e-78	285.00
IUUC-Aor-009914	Aspergillus oryzae	33.21	3.00e-78	285.00
IUUC-Ate-010450	Aspergillus terreus	32.61	1.00e-73	270.00
IUUC-Bgr-012111	Blumeria graminis	33.83	2.00e-63	236.00
IUUC-Cel-018456	Caenorhabditis elegans	32.77	2.00e-75	276.00
IUUC-Dor-030513	Dipodomys ordii	32.38	2.00e-35	143.00
IUUC-Eeu-034713	Erinaceus europaeus	32.16	9.00e-37	147.00
IUUC-Gmo-038562	Gadus morhua	40.91	2.00e-121	428.00
IUUC-Lpe-051535	Leersia perrieri	64.63	0.00e+00	654.00
IUUC-Mac-064521	Musa acuminata	70.25	0.00e+00	798.00
IUUC-Mlu-067459	Myotis lucifugus	40.80	2.00e-124	439.00
IUUC-Nfi-068245	Neosartorya fischeri	32.66	6.00e-73	268.00
IUUC-Oba-075437	Oryza barthii	67.82	0.00e+00	712.00
IUUC-Ogl-077170	Oryza glaberrima	67.82	0.00e+00	712.00
IUUC-Ogu-078844	Oryza glumaepatula	65.86	0.00e+00	655.00
IUUC-Oin-079105	Oryza indica	67.63	0.00e+00	707.00
IUUC-Olo-080943	Oryza longistaminata	62.92	0.00e+00	635.00
IUUC-Oni-082891	Oryza nivara	65.51	0.00e+00	682.00
IUUC-Oru-084960	Oryza rufipogon	62.92	0.00e+00	636.00
IUUC-Osa-085358	Oryza sativa	67.44	0.00e+00	704.00
IUUC-Pno-095084	Phaeosphaeria nodorum	30.90	2.00e-64	240.00
IUUC-Ppa-095798	Physcomitrella patens	56.65	0.00e+00	633.00
IUUC-Ppe-101280	Prunus persica	79.77	0.00e+00	889.00
IUUC-Spo-108276	Schizosaccharomyces pombe	32.12	3.00e-81	295.00
IUUC-Ssl-108387	Sclerotinia sclerotiorum	32.66	2.00e-61	229.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence