iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Ani-009481

Ensembl Protein ID

CADANIAP00000851

UniProt Accession

C8VDI2; Q5AVG2; ATG3_EMENI

Genbank Protein ID

EAA61233.1; CBF79968.1

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3

Genbank Nucleotide ID

AACD01000131; BN001304

Gene Name

ANIA_11004; atg3; AN11004

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
CADANIAG00000851	CADANIAT00000851	CADANIAP00000851

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	37.72	2.00e-31	134.00	IUUC-Ath-006802
E2/Autophagy_C	51.76	2.00e-16	84.70	IUUC-Hsa-046449
E2/Autophagy_C	34.85	1.00e-52	201.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
247	Glycyl thioester intermediate	{ECO:0000250}

Organism

Aspergillus nidulans

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNILHSTLST WRDRLAPVSR TSTFRTTGQI TPEEFVLAGD YLVYKFPSWS WGDASSPSKR 60
VSYLPPGKQF LVTRGVPCHR RLNENFAGDA HLDDEIVRDF LSGGAGDSDG GDDNDGWLRT 120

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Nucleotide Sequence
(Fasta)

ATGAACATCC TCCACTCGAC ATTATCCACC TGGCGTGACC GCCTCGCCCC CGTCTCACGA 60
ACGTCCACCT TCCGCACTAC CGGCCAGATC ACACCTGAAG AATTCGTCCT TGCCGGCGAC 120

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Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0044804--P:nucleophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	49.35	2.00e-17	81.30
IUUC-Aml-002081	Ailuropoda melanoleuca	51.39	7.00e-18	82.80
IUUC-Atr-002712	Amborella trichopoda	41.56	4.00e-13	66.60
IUUC-Apl-003956	Anas platyrhynchos	49.12	4.00e-11	60.10
IUUC-Aca-004578	Anolis carolinensis	50.00	6.00e-17	79.70
IUUC-Aly-005823	Arabidopsis lyrata	36.19	2.00e-32	131.00
IUUC-Ath-006802	Arabidopsis thaliana	35.13	1.00e-31	128.00
IUUC-Ago-007855	Ashbya gossypii	38.46	2.00e-32	130.00
IUUC-Acl-008147	Aspergillus clavatus	72.92	1.00e-126	444.00
IUUC-Afl-008483	Aspergillus flavus	78.53	8.00e-134	468.00
IUUC-Afu-008818	Aspergillus fumigatus	77.51	4.00e-147	512.00
IUUC-Ang-009800	Aspergillus niger	68.02	7.00e-123	431.00
IUUC-Aor-010150	Aspergillus oryzae	78.53	8.00e-134	468.00
IUUC-Ate-010545	Aspergillus terreus	73.20	3.00e-75	273.00
IUUC-Ame-011793	Astyanax mexicanus	51.43	5.00e-17	80.10
IUUC-Bgr-012167	Blumeria graminis	60.32	3.00e-101	359.00
IUUC-Bta-012695	Bos taurus	51.39	7.00e-18	82.80
IUUC-Bci-013972	Botrytis cinerea	64.25	5.00e-98	348.00
IUUC-Bdi-014756	Brachypodium distachyon	50.65	3.00e-18	84.00
IUUC-Bol-015091	Brassica oleracea	49.35	1.00e-17	81.60
IUUC-Bra-017220	Brassica rapa	35.97	2.00e-32	130.00
IUUC-Cel-018143	Caenorhabditis elegans	50.00	8.00e-21	92.40
IUUC-Cja-018991	Callithrix jacchus	49.44	4.00e-17	80.10
IUUC-Cfa-020070	Canis familiaris	51.39	8.00e-18	82.40
IUUC-Cpo-021860	Cavia porcellus	52.78	1.00e-18	85.10
IUUC-Cre-022948	Chlamydomonas reinhardtii	46.15	1.00e-16	78.60
IUUC-Csa-023881	Chlorocebus sabaeus	51.39	7.00e-18	82.80
IUUC-Cho-024852	Choloepus hoffmanni	50.00	4.00e-17	80.10
IUUC-Cin-025787	Ciona intestinalis	48.05	1.00e-18	84.70
IUUC-Csv-026369	Ciona savignyi	53.16	1.00e-20	92.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	56.99	6.00e-92	328.00
IUUC-Cne-026905	Cryptococcus neoformans	34.95	1.00e-20	92.40
IUUC-Dre-028811	Danio rerio	51.43	5.00e-17	80.10
IUUC-Dno-030081	Dasypus novemcinctus	50.65	6.00e-17	79.70
IUUC-Dor-030183	Dipodomys ordii	51.39	5.00e-18	83.20
IUUC-Dse-031539	Dothistroma septosporum	50.81	8.00e-62	228.00
IUUC-Dme-031805	Drosophila melanogaster	47.62	1.00e-18	85.10
IUUC-Ete-033171	Echinops telfairi	51.39	3.00e-18	83.60
IUUC-Eca-034083	Equus caballus	51.39	8.00e-18	82.40
IUUC-Eeu-034804	Erinaceus europaeus	52.78	5.00e-18	83.20
IUUC-Fca-036289	Felis catus	51.39	8.00e-18	82.40
IUUC-Fal-037060	Ficedula albicollis	50.65	4.00e-17	80.10
IUUC-Fox-038051	Fusarium oxysporum	56.25	6.00e-98	348.00
IUUC-Fso-038124	Fusarium solani	59.40	3.00e-87	313.00
IUUC-Gmo-039538	Gadus morhua	50.65	1.00e-16	79.00
IUUC-Ggr-039880	Gaeumannomyces graminis	57.14	1.00e-103	368.00
IUUC-Gga-041108	Gallus gallus	50.65	5.00e-17	80.10
IUUC-Gac-041558	Gasterosteus aculeatus	35.29	7.00e-31	125.00
IUUC-Gma-043969	Glycine max	46.75	6.00e-17	79.70
IUUC-Ggo-045071	Gorilla gorilla	51.39	8.00e-18	82.40
IUUC-Hsa-046449	Homo sapiens	51.39	8.00e-18	82.40
IUUC-Hvu-047451	Hordeum vulgare	49.35	2.00e-17	80.90
IUUC-Itr-048360	Ictidomys tridecemlineatus	51.39	7.00e-18	82.80
IUUC-Kpa-049096	Komagataella pastoris	39.56	2.00e-32	130.00
IUUC-Lch-049726	Latimeria chalumnae	50.65	4.00e-17	80.10
IUUC-Lpe-050734	Leersia perrieri	34.43	7.00e-34	135.00
IUUC-Loc-051983	Lepisosteus oculatus	51.43	6.00e-17	79.70
IUUC-Lma-053038	Leptosphaeria maculans	59.34	2.00e-104	370.00
IUUC-Laf-054510	Loxodonta africana	49.44	3.00e-17	80.50
IUUC-Mcc-054792	Macaca mulatta	51.39	7.00e-18	82.80
IUUC-Meu-055965	Macropus eugenii	47.83	2.00e-17	80.90
IUUC-Mor-057292	Magnaporthe oryzae	58.70	5.00e-98	348.00
IUUC-Mtr-058282	Medicago truncatula	34.64	1.00e-32	131.00
IUUC-Mla-059144	Melampsora laricipopulina	40.22	7.00e-26	109.00
IUUC-Mga-059509	Meleagris gallopavo	50.65	5.00e-17	79.70
IUUC-Mvi-060281	Microbotryum violaceum	38.71	2.00e-24	104.00
IUUC-Mmr-061788	Microcebus murinus	51.39	6.00e-18	82.80
IUUC-Mdo-062922	Monodelphis domestica	51.39	1.00e-17	82.00
IUUC-Mmu-064249	Mus musculus	49.44	4.00e-17	80.10
IUUC-Mac-064843	Musa acuminata	49.35	1.00e-17	81.60
IUUC-Mpu-065717	Mustela putorius furo	51.39	8.00e-18	82.40
IUUC-Mlu-067151	Myotis lucifugus	55.56	2.00e-16	77.80
IUUC-Nfi-068551	Neosartorya fischeri	76.36	1.00e-143	500.00
IUUC-Ncr-068846	Neurospora crassa	59.13	1.00e-95	341.00
IUUC-Nle-069790	Nomascus leucogenys	51.39	8.00e-18	82.40
IUUC-Ont-072517	Oreochromis niloticus	50.65	4.00e-17	80.10
IUUC-Oan-073285	Ornithorhynchus anatinus	50.65	5.00e-17	79.30
IUUC-Ocu-074695	Oryctolagus cuniculus	51.39	7.00e-18	82.40
IUUC-Oba-074991	Oryza barthii	35.11	1.00e-33	135.00
IUUC-Obr-076787	Oryza brachyantha	48.05	2.00e-17	81.30
IUUC-Ogl-077440	Oryza glaberrima	34.07	6.00e-33	132.00
IUUC-Ogu-078294	Oryza glumaepatula	35.23	3.00e-33	134.00
IUUC-Oin-079452	Oryza indica	34.56	3.00e-32	130.00
IUUC-Olo-080450	Oryza longistaminata	36.22	4.00e-34	136.00
IUUC-Ome-081514	Oryza meridionalis	35.09	6.00e-33	132.00
IUUC-Oni-082677	Oryza nivara	34.56	3.00e-32	130.00
IUUC-Opu-083925	Oryza punctata	49.35	7.00e-18	82.80
IUUC-Oru-084828	Oryza rufipogon	38.10	3.00e-33	134.00
IUUC-Osa-085937	Oryza sativa	34.56	3.00e-32	130.00
IUUC-Ola-086559	Oryzias latipes	46.43	4.00e-18	82.80
IUUC-Olu-087766	Ostreococcus lucimarinus	32.85	6.00e-28	115.00
IUUC-Oga-087983	Otolemur garnettii	49.44	4.00e-17	80.10
IUUC-Oar-089281	Ovis aries	51.39	7.00e-18	82.80
IUUC-Ptr-090983	Pan troglodytes	51.39	8.00e-18	82.40
IUUC-Pan-092457	Papio anubis	51.39	7.00e-18	82.80
IUUC-Psi-094046	Pelodiscus sinensis	50.65	8.00e-17	79.00
IUUC-Pno-094925	Phaeosphaeria nodorum	58.52	3.00e-101	360.00
IUUC-Ppa-095487	Physcomitrella patens	48.72	3.00e-17	80.50
IUUC-Pfo-096968	Poecilia formosa	45.24	4.00e-17	80.10
IUUC-Pab-098621	Pongo abelii	51.39	8.00e-18	82.40
IUUC-Pop-100007	Populus trichocarpa	48.05	5.00e-17	80.10
IUUC-Pca-100590	Procavia capensis	49.44	5.00e-17	79.70
IUUC-Ppe-101657	Prunus persica	46.75	3.00e-16	77.00
IUUC-Pva-102446	Pteropus vampyrus	51.39	7.00e-18	82.40
IUUC-Pgr-103604	Puccinia graminis	37.10	1.00e-24	105.00
IUUC-Ptt-103941	Puccinia triticina	36.76	8.00e-26	109.00
IUUC-Pte-104252	Pyrenophora teres	58.52	4.00e-103	365.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	59.07	3.00e-104	370.00
IUUC-Rno-106025	Rattus norvegicus	51.39	8.00e-18	82.40
IUUC-Sce-106172	Saccharomyces cerevisiae	36.74	1.00e-42	164.00
IUUC-Sha-106858	Sarcophilus harrisii	51.39	7.00e-18	82.80
IUUC-Sja-107905	Schizosaccharomyces japonicus	51.32	2.00e-17	81.30
IUUC-Spo-108185	Schizosaccharomyces pombe	53.75	1.00e-18	84.70
IUUC-Smo-108883	Selaginella moellendorffii	47.44	6.00e-16	76.30
IUUC-Sit-110512	Setaria italica	49.35	5.00e-17	80.10
IUUC-Sly-111238	Solanum lycopersicum	48.05	4.00e-17	80.10
IUUC-Stu-112441	Solanum tuberosum	48.05	4.00e-17	80.10
IUUC-Sar-113039	Sorex araneus	49.44	4.00e-17	79.70
IUUC-Sbi-113776	Sorghum bicolor	49.35	2.00e-17	80.90
IUUC-Sre-115074	Sporisorium reilianum	51.19	1.00e-19	89.40
IUUC-Ssc-115262	Sus scrofa	51.39	8.00e-18	82.40
IUUC-Tgu-116790	Taeniopygia guttata	50.65	5.00e-17	80.10
IUUC-Tru-118160	Takifugu rubripes	49.41	2.00e-18	84.70
IUUC-Tsy-119145	Tarsius syrichta	56.76	9.00e-08	48.90
IUUC-Tni-119937	Tetraodon nigroviridis	49.35	5.00e-16	76.60
IUUC-Tca-121323	Theobroma cacao	48.05	4.00e-17	80.10
IUUC-Tre-121993	Trichoderma reesei	56.91	2.00e-87	313.00
IUUC-Tvi-122332	Trichoderma virens	52.85	3.00e-87	313.00
IUUC-Tae-124391	Triticum aestivum	49.35	3.00e-17	80.50
IUUC-Tur-126679	Triticum urartu	49.35	3.00e-17	80.50
IUUC-Tme-127159	Tuber melanosporum	55.17	4.00e-19	86.30
IUUC-Ttr-128261	Tursiops truncatus	49.44	4.00e-17	80.10
IUUC-Uma-129572	Ustilago maydis	32.39	3.00e-23	100.00
IUUC-Vda-129966	Verticillium dahliae	60.70	1.00e-101	360.00
IUUC-Vpa-130698	Vicugna pacos	49.44	5.00e-17	80.10
IUUC-Xtr-132878	Xenopus tropicalis	35.85	9.00e-32	129.00
IUUC-Xma-134017	Xiphophorus maculatus	45.24	4.00e-17	80.10
IUUC-Yli-134373	Yarrowia lipolytica	62.20	8.00e-23	99.40
IUUC-Zma-135222	Zea mays	49.35	5.00e-17	80.10

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence