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Tag Content
iUUCD ID IUUC-Bra-017506
Ensembl Protein ID Bra005592.1-P
UniProt Accession M4CN04; M4CN04_BRARP
Protein Name NEDD8-activating enzyme E1 regulatory subunit
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
Bra005592 Bra005592.1 Bra005592.1-P
Status Unreviewed
Classification
Family E-Value Score Start End
E1 9.50e-117 391.7 9 516
Active Site
Position(s) Description Evidence
N/A N/A N/A
Domain Profile

   E1

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveahees 96
    YDRQ+r+wGe++q++l++a+++l+++g++G+E lKnLvl+G+gs+tvvD+++ve+ +l+++F++++++vg sKA++++++l+eln+ v++ +++e+
   Q: 9 YDRQLRIWGEVGQAALENASICLLNCGPTGSEALKNLVLGGIGSVTVVDGSKVEIGDLGNNFMVDKKSVGESKAKTVCAFLQELNDAVRANFVQEN 104
    ************************************************************************************************ PP
   S: 97 iteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn....... 181
    ++ l +++ Ff++f++Vv+++ ++++ k++r+c+ a+v l+ ++++Gl G vr+++k++t+++s++d d++++++P+++lk +++
   Q: 105 PDSLILTDPsFFSQFTLVVATQLVEDSMVKLDRICRGAKVMLVFARSYGLTGLVRISVKEHTIIDSKPDhflDDLRLNNPWPELKRFVEtidlktp 200
    ***************************************************************************************999999999 PP
   S: 182 ..aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv 274
    a+++i+++v++ k+++e+a+ ++ + ++++eek+e+kd+vks+++s+de+n++ea+e+++k fa++ i++ i++++++ + ++v +++s+fwv
   Q: 201 dpIAHKHIPYVVILVKMADEWAKTHSSNLPSTREEKREFKDLVKSKMVSMDEDNYKEAVEATFKVFAPRGISKeIQEIIDD-RYAEVGSNSSAFWV 295
    999**********************************************************************99999999.999*********** PP
   S: 275 ...........................................................................sfcsnaealq........... 284
    sfc+na++l+
   Q: 296 mvaalkefisnegdgeaplegsmpdmtsstehyinlqkiyhtkaeddclsmeqrvkdilakvgrdpssiskqtikSFCKNARKLKvcryrtiedef 391
    *************************************************************************************77777776666 PP
   S: 285 vpekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattna 374
    +++++++ ++ ++++++ +y+llra++rf+++++ pg+++ + +++a +l + a +l+ ++g+ + +l+++l e+++++++++++++a
   Q: 392 SNPSAAELQKCLASEDYssaigFYILLRAVDRFAATYKNFPGQFDGGDREEDASRLRAIAVSLI-NEMGCDGyELPEELCNEMCRFGAAELHVVAA 486
    55666666666666666899******************************99999999888888.******************************* PP
   S: 375 vvgGlaaqEviKvvsgkfeplknfflfdae 404
    +vgG+a+qEviK+++++f+p+ +f+f+++
   Q: 487 FVGGIASQEVIKLITKQFVPMLGTFVFNGI 516
    **************************9986 PP
   

Organism Brassica rapa
Functional Description
(View)

Functional Description



     Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.
Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.
Protein Sequence
(Fasta)
MSEPTKTKYD RQLRIWGEVG QAALENASIC LLNCGPTGSE ALKNLVLGGI GSVTVVDGSK 60
VEIGDLGNNF MVDKKSVGES KAKTVCAFLQ ELNDAVRANF VQENPDSLIL TDPSFFSQFT 120
It may take some time, please wait.

Protein Fasta Sequence



>IUUC-Bra-017506|E1,E1_ThiF|Brassica rapa
Please wait for a moment...
Nucleotide Sequence
(Fasta)
ATGTCTGAGC CCACGAAAAC CAAGTACGAT CGTCAACTCA GGTAACCCTC CAGCTCTTGA 60
CTTCCCCTCC GTTTCCGATC GCTCCAAGTC TTCATCTTGT GATTGTTTCT TAGGATCTGG 120
It may take some time, please wait.

Nucleotide Fasta Sequence



>IUUC-Bra-017506|E1,E1_ThiF|Brassica rapa
Please wait for a moment...
Sequence Source Ensembl
Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0005777--C:peroxisome
GO:0019781--F:NEDD8 activating enzyme activity
GO:0009791--P:post-embryonic development
GO:0045116--P:protein neddylation

Orthology
iUUCD ID Species Identity E-value Score
IUUC-Ata-000764 Aegilops tauschii 64.90 0.00e+00 719.00
IUUC-Aml-002333 Ailuropoda melanoleuca 38.66 2.00e-118 419.00
IUUC-Atr-002644 Amborella trichopoda 69.33 0.00e+00 735.00
IUUC-Apl-003446 Anas platyrhynchos 38.58 2.00e-110 392.00
IUUC-Aly-006187 Arabidopsis lyrata 83.81 0.00e+00 916.00
IUUC-Ath-007600 Arabidopsis thaliana 81.90 0.00e+00 894.00
IUUC-Ago-007900 Ashbya gossypii 25.98 1.00e-34 140.00
IUUC-Acl-008333 Aspergillus clavatus 32.60 4.00e-68 252.00
IUUC-Afl-008529 Aspergillus flavus 33.03 5.00e-75 275.00
IUUC-Afu-009071 Aspergillus fumigatus 32.36 3.00e-68 252.00
IUUC-Ani-009443 Aspergillus nidulans 31.00 3.00e-64 239.00
IUUC-Ang-009669 Aspergillus niger 31.94 9.00e-73 267.00
IUUC-Aor-009914 Aspergillus oryzae 33.57 5.00e-74 271.00
IUUC-Ate-010450 Aspergillus terreus 32.19 9.00e-69 254.00
IUUC-Ame-011183 Astyanax mexicanus 40.04 7.00e-121 427.00
IUUC-Bgr-012111 Blumeria graminis 33.40 3.00e-63 235.00
IUUC-Bta-012583 Bos taurus 39.18 1.00e-121 429.00
IUUC-Bci-013892 Botrytis cinerea 31.98 2.00e-56 213.00
IUUC-Bdi-014592 Brachypodium distachyon 65.20 0.00e+00 721.00
IUUC-Bol-015487 Brassica oleracea 96.19 0.00e+00 1053.00
IUUC-Cel-018456 Caenorhabditis elegans 31.61 2.00e-73 269.00
IUUC-Cja-020007 Callithrix jacchus 38.40 1.00e-118 419.00
IUUC-Cfa-020324 Canis familiaris 38.81 7.00e-121 427.00
IUUC-Cpo-022248 Cavia porcellus 34.39 5.00e-63 234.00
IUUC-Csa-023149 Chlorocebus sabaeus 35.90 1.00e-91 330.00
IUUC-Csv-026113 Ciona savignyi 38.49 1.00e-103 369.00
IUUC-Cgl-026428 Colletotrichum gloeosporioides 30.95 3.00e-57 216.00
IUUC-Cne-026884 Cryptococcus neoformans 31.17 1.00e-68 253.00
IUUC-Cme-027210 Cyanidioschyzon merolae 25.11 3.00e-15 76.60
IUUC-Dre-028140 Danio rerio 40.97 5.00e-124 437.00
IUUC-Dno-030033 Dasypus novemcinctus 38.23 2.00e-103 369.00
IUUC-Dor-030513 Dipodomys ordii 50.91 9.00e-27 114.00
IUUC-Dse-031536 Dothistroma septosporum 32.97 1.00e-73 270.00
IUUC-Dme-031683 Drosophila melanogaster 36.07 1.00e-96 347.00
IUUC-Eca-033992 Equus caballus 36.40 9.00e-92 330.00
IUUC-Fca-036548 Felis catus 38.53 2.00e-118 419.00
IUUC-Fal-037600 Ficedula albicollis 39.28 2.00e-111 395.00
IUUC-Fox-038037 Fusarium oxysporum 30.61 1.00e-60 227.00
IUUC-Fso-038387 Fusarium solani 31.37 3.00e-64 239.00
IUUC-Gmo-038562 Gadus morhua 38.75 7.00e-117 414.00
IUUC-Ggr-040005 Gaeumannomyces graminis 31.62 1.00e-59 224.00
IUUC-Gga-041129 Gallus gallus 38.18 1.00e-112 399.00
IUUC-Gac-042558 Gasterosteus aculeatus 39.09 1.00e-117 416.00
IUUC-Ggo-045611 Gorilla gorilla 38.83 1.00e-117 416.00
IUUC-Hsa-046563 Homo sapiens 39.05 8.00e-119 420.00
IUUC-Hvu-047790 Hordeum vulgare 62.82 3.00e-148 517.00
IUUC-Itr-048066 Ictidomys tridecemlineatus 38.81 2.00e-119 422.00
IUUC-Kpa-049309 Komagataella pastoris 34.70 2.00e-75 276.00
IUUC-Lch-050038 Latimeria chalumnae 39.16 6.00e-114 404.00
IUUC-Lpe-051535 Leersia perrieri 62.90 0.00e+00 633.00
IUUC-Loc-051917 Lepisosteus oculatus 37.13 3.00e-105 375.00
IUUC-Lma-053112 Leptosphaeria maculans 34.13 6.00e-78 284.00
IUUC-Laf-053690 Loxodonta africana 39.32 4.00e-121 427.00
IUUC-Mcc-055117 Macaca mulatta 38.15 5.00e-115 407.00
IUUC-Meu-056761 Macropus eugenii 32.02 2.00e-84 306.00
IUUC-Mor-057230 Magnaporthe oryzae 32.41 9.00e-66 244.00
IUUC-Mpo-057514 Magnaporthe poae 31.52 3.00e-59 222.00
IUUC-Mtr-058551 Medicago truncatula 69.50 0.00e+00 761.00
IUUC-Mla-059183 Melampsora laricipopulina 33.46 5.00e-84 305.00
IUUC-Mga-060144 Meleagris gallopavo 38.12 3.00e-109 388.00
IUUC-Mvi-060221 Microbotryum violaceum 32.82 8.00e-73 267.00
IUUC-Mmr-061325 Microcebus murinus 37.48 3.00e-114 405.00
IUUC-Mdo-062532 Monodelphis domestica 39.32 4.00e-120 424.00
IUUC-Mmu-063956 Mus musculus 38.99 7.00e-121 427.00
IUUC-Mac-064521 Musa acuminata 69.47 0.00e+00 774.00
IUUC-Mpu-066716 Mustela putorius furo 38.29 3.00e-110 392.00
IUUC-Mlu-067459 Myotis lucifugus 39.21 3.00e-121 428.00
IUUC-Nfi-068245 Neosartorya fischeri 32.36 5.00e-69 254.00
IUUC-Ncr-068910 Neurospora crassa 33.15 1.00e-70 260.00
IUUC-Nle-070034 Nomascus leucogenys 37.29 9.00e-113 400.00
IUUC-Opr-070552 Ochotona princeps 35.79 1.00e-91 330.00
IUUC-Ont-071426 Oreochromis niloticus 39.85 2.00e-121 429.00
IUUC-Oan-073389 Ornithorhynchus anatinus 36.97 5.00e-87 314.00
IUUC-Ocu-074911 Oryctolagus cuniculus 39.37 6.00e-122 431.00
IUUC-Oba-075437 Oryza barthii 66.54 0.00e+00 692.00
IUUC-Obr-076684 Oryza brachyantha 66.35 0.00e+00 656.00
IUUC-Ogl-077170 Oryza glaberrima 66.54 0.00e+00 692.00
IUUC-Ogu-078844 Oryza glumaepatula 65.33 0.00e+00 639.00
IUUC-Oin-079105 Oryza indica 66.35 0.00e+00 688.00
IUUC-Olo-080943 Oryza longistaminata 62.04 3.00e-178 617.00
IUUC-Oni-082891 Oryza nivara 64.24 0.00e+00 662.00
IUUC-Opu-083457 Oryza punctata 66.35 0.00e+00 690.00
IUUC-Oru-084960 Oryza rufipogon 61.79 3.00e-178 617.00
IUUC-Osa-085358 Oryza sativa 66.16 0.00e+00 685.00
IUUC-Olu-087625 Ostreococcus lucimarinus 31.84 6.00e-63 234.00
IUUC-Oga-088810 Otolemur garnettii 38.99 1.00e-120 426.00
IUUC-Oar-089196 Ovis aries 38.96 5.00e-120 424.00
IUUC-Ptr-090617 Pan troglodytes 39.05 4.00e-119 421.00
IUUC-Pan-092761 Papio anubis 38.81 3.00e-120 425.00
IUUC-Psi-093191 Pelodiscus sinensis 36.61 2.00e-112 398.00
IUUC-Pma-094380 Petromyzon marinus 39.20 5.00e-106 377.00
IUUC-Pno-095084 Phaeosphaeria nodorum 30.77 2.00e-62 234.00
IUUC-Ppa-095798 Physcomitrella patens 54.84 4.00e-177 613.00
IUUC-Pfo-096467 Poecilia formosa 39.62 4.00e-117 414.00
IUUC-Pab-098255 Pongo abelii 38.88 3.00e-101 361.00
IUUC-Pop-099722 Populus trichocarpa 74.29 0.00e+00 828.00
IUUC-Pca-100807 Procavia capensis 47.31 4.00e-50 191.00
IUUC-Ppe-101280 Prunus persica 75.24 0.00e+00 844.00
IUUC-Pva-102333 Pteropus vampyrus 37.89 2.00e-111 395.00
IUUC-Pgr-103401 Puccinia graminis 28.97 5.00e-73 268.00
IUUC-Ptt-103889 Puccinia triticina 27.35 1.00e-60 226.00
IUUC-Pte-104355 Pyrenophora teres 34.49 2.00e-75 276.00
IUUC-Pyt-104545 Pyrenophora triticirepentis 34.80 1.00e-74 273.00
IUUC-Rno-105810 Rattus norvegicus 38.86 1.00e-118 419.00
IUUC-Sha-106770 Sarcophilus harrisii 44.11 2.00e-67 248.00
IUUC-Sja-107926 Schizosaccharomyces japonicus 30.84 2.00e-74 272.00
IUUC-Spo-108276 Schizosaccharomyces pombe 32.25 3.00e-82 299.00
IUUC-Ssl-108387 Sclerotinia sclerotiorum 33.88 1.00e-58 220.00
IUUC-Smo-109296 Selaginella moellendorffii 53.76 5.00e-164 570.00
IUUC-Sit-110157 Setaria italica 66.29 0.00e+00 756.00
IUUC-Sly-111105 Solanum lycopersicum 70.50 0.00e+00 802.00
IUUC-Stu-112469 Solanum tuberosum 71.99 0.00e+00 817.00
IUUC-Sar-113083 Sorex araneus 31.85 1.00e-81 296.00
IUUC-Sbi-113813 Sorghum bicolor 65.52 0.00e+00 745.00
IUUC-Sre-115058 Sporisorium reilianum 33.11 3.00e-63 235.00
IUUC-Tgu-116772 Taeniopygia guttata 40.30 5.00e-113 401.00
IUUC-Tru-118686 Takifugu rubripes 39.09 2.00e-120 425.00
IUUC-Tsy-119111 Tarsius syrichta 41.03 8.00e-59 221.00
IUUC-Tni-120231 Tetraodon nigroviridis 36.79 2.00e-104 372.00
IUUC-Tre-122103 Trichoderma reesei 33.39 1.00e-70 260.00
IUUC-Tvi-122672 Trichoderma virens 33.52 3.00e-66 245.00
IUUC-Tae-125684 Triticum aestivum 64.90 0.00e+00 721.00
IUUC-Tur-126487 Triticum urartu 64.02 0.00e+00 701.00
IUUC-Tme-127097 Tuber melanosporum 35.78 9.00e-83 300.00
IUUC-Ttr-128465 Tursiops truncatus 36.36 1.00e-106 380.00
IUUC-Uma-129645 Ustilago maydis 30.60 4.00e-69 255.00
IUUC-Vda-129989 Verticillium dahliae 26.90 7.00e-47 181.00
IUUC-Vpa-130529 Vicugna pacos 37.48 2.00e-110 392.00
IUUC-Vvi-131059 Vitis vinifera 76.00 0.00e+00 834.00
IUUC-Xtr-132002 Xenopus tropicalis 40.76 1.00e-122 432.00
IUUC-Xma-133930 Xiphophorus maculatus 39.92 7.00e-121 427.00
IUUC-Yli-134505 Yarrowia lipolytica 34.43 1.00e-82 300.00
IUUC-Zma-135619 Zea mays 65.14 0.00e+00 703.00
Created Date 25-Jun-2017

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