iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Xma-133620

Ensembl Protein ID

ENSXMAP00000017393.1

UniProt Accession

M4ASE8; M4ASE8_XIPMA

Genbank Protein ID

ENSXMAP00000017393

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Genbank Nucleotide ID

AGAJ01014228

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSXMAG00000017358.1	ENSXMAT00000017417.1	ENSXMAP00000017393.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	6.00e-07	29.9	382	420

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+  C+++w       +k++ CP+Cr
   Q: 382  CIICSELFIE-AVILNCAHSFCCYCIKQW------RNKKDECPICR 420
           ********99.888***************......**********8 PP

Organism

Xiphophorus maculatus

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MEGVTTDCCS AEEDDCSDSE VLCLMRVGET SDWLRLFENA EVTVGRGVEV THQLLSSTCP 60
LMISRLHCSF RQKEDGQWTV TDKKSLNGVW VNGERIPAEQ PHLLQLGDSI QLGVPLLGTK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGGAGGGCG TAACAACGGA TTGCTGCTCG GCTGAGGAGG ATGACTGCTC AGACTCGGAG 60
GTTTTATGTC TCATGAGAGT CGGAGAAACC TCGGACTGGC TCCGTTTGTT CGAAAACGCA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000996	Aegilops tauschii	36.23	2.00e-08	52.40
IUUC-Aml-002124	Ailuropoda melanoleuca	39.62	2.00e-61	229.00
IUUC-Atr-002441	Amborella trichopoda	31.03	8.00e-09	53.90
IUUC-Apl-003664	Anas platyrhynchos	69.32	7.00e-32	129.00
IUUC-Aca-005036	Anolis carolinensis	41.41	1.00e-68	253.00
IUUC-Ame-011537	Astyanax mexicanus	53.39	1.00e-112	400.00
IUUC-Bta-013297	Bos taurus	64.29	3.00e-38	153.00
IUUC-Bdi-014630	Brachypodium distachyon	40.00	8.00e-08	50.80
IUUC-Bra-016994	Brassica rapa	30.93	4.00e-07	48.10
IUUC-Cja-019201	Callithrix jacchus	40.64	8.00e-68	251.00
IUUC-Cfa-021041	Canis familiaris	39.96	2.00e-65	243.00
IUUC-Cre-022788	Chlamydomonas reinhardtii	27.27	2.00e-09	57.00
IUUC-Csa-024133	Chlorocebus sabaeus	42.44	9.00e-67	247.00
IUUC-Cho-024624	Choloepus hoffmanni	34.57	7.00e-26	111.00
IUUC-Dre-027918	Danio rerio	55.51	2.00e-118	419.00
IUUC-Dno-029774	Dasypus novemcinctus	41.16	8.00e-69	254.00
IUUC-Dor-030190	Dipodomys ordii	35.75	7.00e-49	188.00
IUUC-Ete-032301	Echinops telfairi	37.62	1.00e-43	170.00
IUUC-Eca-033607	Equus caballus	40.18	2.00e-57	216.00
IUUC-Eeu-034888	Erinaceus europaeus	43.05	3.00e-42	166.00
IUUC-Fca-036075	Felis catus	42.76	2.00e-68	253.00
IUUC-Fal-037569	Ficedula albicollis	39.95	3.00e-61	229.00
IUUC-Gmo-038588	Gadus morhua	56.96	9.00e-131	460.00
IUUC-Gga-040777	Gallus gallus	44.35	1.00e-69	257.00
IUUC-Gac-042463	Gasterosteus aculeatus	77.01	4.00e-157	548.00
IUUC-Ggo-045269	Gorilla gorilla	40.46	9.00e-68	251.00
IUUC-Hsa-046249	Homo sapiens	40.94	1.00e-63	237.00
IUUC-Hvu-047324	Hordeum vulgare	36.23	1.00e-08	53.50
IUUC-Itr-048314	Ictidomys tridecemlineatus	41.14	5.00e-70	258.00
IUUC-Lch-049805	Latimeria chalumnae	44.21	3.00e-80	292.00
IUUC-Lpe-050730	Leersia perrieri	28.45	1.00e-09	57.00
IUUC-Loc-052377	Lepisosteus oculatus	50.96	3.00e-102	365.00
IUUC-Mcc-055344	Macaca mulatta	42.95	8.00e-68	251.00
IUUC-Meu-055950	Macropus eugenii	40.85	3.00e-58	219.00
IUUC-Mga-060049	Meleagris gallopavo	64.46	6.00e-45	175.00
IUUC-Mmr-061431	Microcebus murinus	40.09	4.00e-66	245.00
IUUC-Mdo-062016	Monodelphis domestica	42.76	4.00e-62	234.00
IUUC-Mmu-063368	Mus musculus	39.75	5.00e-77	282.00
IUUC-Mpu-066449	Mustela putorius furo	39.59	2.00e-69	256.00
IUUC-Mlu-067588	Myotis lucifugus	41.68	4.00e-62	232.00
IUUC-Nle-069192	Nomascus leucogenys	40.25	1.00e-67	250.00
IUUC-Opr-070300	Ochotona princeps	33.33	4.00e-53	202.00
IUUC-Ont-071998	Oreochromis niloticus	79.43	4.00e-175	607.00
IUUC-Oan-073610	Ornithorhynchus anatinus	55.36	1.00e-45	176.00
IUUC-Ocu-074603	Oryctolagus cuniculus	41.36	7.00e-68	251.00
IUUC-Oba-075867	Oryza barthii	26.72	2.00e-09	56.20
IUUC-Obr-076083	Oryza brachyantha	28.04	3.00e-09	55.50
IUUC-Ogl-077580	Oryza glaberrima	26.72	3.00e-09	55.10
IUUC-Ogu-078162	Oryza glumaepatula	26.72	5.00e-09	54.70
IUUC-Oin-079986	Oryza indica	27.73	7.00e-09	54.30
IUUC-Olo-080306	Oryza longistaminata	27.59	9.00e-10	57.00
IUUC-Ome-081802	Oryza meridionalis	26.72	3.00e-09	56.20
IUUC-Opu-084086	Oryza punctata	27.59	2.00e-09	56.20
IUUC-Oru-084301	Oryza rufipogon	27.73	6.00e-09	54.30
IUUC-Ola-086681	Oryzias latipes	64.19	1.00e-154	539.00
IUUC-Oga-088183	Otolemur garnettii	40.93	6.00e-71	261.00
IUUC-Oar-090255	Ovis aries	63.57	3.00e-37	149.00
IUUC-Ptr-091029	Pan troglodytes	40.74	9.00e-64	237.00
IUUC-Pan-092241	Papio anubis	42.65	4.00e-68	252.00
IUUC-Psi-093506	Pelodiscus sinensis	59.09	5.00e-26	112.00
IUUC-Pma-094579	Petromyzon marinus	54.44	9.00e-25	107.00
IUUC-Pfo-097014	Poecilia formosa	94.46	0.00e+00	732.00
IUUC-Pab-098530	Pongo abelii	40.26	3.00e-64	239.00
IUUC-Pca-100939	Procavia capensis	33.90	4.00e-38	152.00
IUUC-Ppe-101294	Prunus persica	29.47	4.00e-08	51.60
IUUC-Pva-103148	Pteropus vampyrus	63.04	1.00e-31	130.00
IUUC-Rno-105338	Rattus norvegicus	41.86	4.00e-78	285.00
IUUC-Sha-107187	Sarcophilus harrisii	64.07	3.00e-45	176.00
IUUC-Sit-110346	Setaria italica	25.00	3.00e-08	52.00
IUUC-Sar-113356	Sorex araneus	36.70	1.00e-44	173.00
IUUC-Sre-115193	Sporisorium reilianum	37.50	4.00e-06	47.00
IUUC-Ssc-116290	Sus scrofa	40.96	1.00e-72	266.00
IUUC-Tru-117910	Takifugu rubripes	72.32	4.00e-166	577.00
IUUC-Tsy-119329	Tarsius syrichta	62.32	5.00e-34	138.00
IUUC-Tni-120521	Tetraodon nigroviridis	69.98	1.00e-153	536.00
IUUC-Tca-121857	Theobroma cacao	38.78	1.00e-07	50.10
IUUC-Tur-126155	Triticum urartu	36.23	2.00e-08	53.50
IUUC-Ttr-128997	Tursiops truncatus	35.03	2.00e-52	199.00
IUUC-Vpa-130154	Vicugna pacos	37.27	4.00e-64	238.00
IUUC-Vvi-131516	Vitis vinifera	41.86	2.00e-07	49.70
IUUC-Xtr-132805	Xenopus tropicalis	42.07	4.00e-69	255.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence