iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
DNA & RNA Element
TRANSFAC
Protein-protein Interaction
HINT	Mentha
Post-translational Modifications (PTMs)
dbPPT	dbPTM	UniProt
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	17644626; 20682785

Classification

Family	E-value	Score	Start	End
E1	3.10e-116	389.9	5	409

Active Site

Position(s)	Description	Evidence
168	Glycyl thioester intermediate	{ECO:0000250}

Domain Profile

   S: 11   eaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfk 108
           ++q ++k akvl+vGagg+GcEllK+L+l+G++ i+++Dmdt+evsnlnrqFLfr+++vg+sKA+va +a+ ++ p++n+ +++ +++++e ++ Ffk
   Q: 5    QQQSAIKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFK 102
           6899**************************************************************************************99999*** PP
   S: 109  kfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsddppqktiPfctlketpnaaehtiewa..vlfnklleeeagee 204
           +fdvV+++ldn++arr+vnrlcl+a+vpl+esgt+G+lGqv+v+ikg+tecy+c+++p  kt+P+ct+ +tp++  h+i+wa   lf+kl++++++++
   Q: 103  QFDVVLNGLDNLDARRHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKFVHCIVWAkdLLFAKLFGDKNQDN 200
           ************************************************************************************************** PP
   S: 205  evlekldseekeegkdkvkselksedeenfeeaieiavkafakttinsikqllksfecdivtkskspfwv..........................sf 276
           + + +++++++++ +++   e +               ++ +  ++++ k +++ f  +i    ++++++                          + 
   Q: 201  DLNVRSNNSASSSKETEDVFE-R---------------SEDEDIEQYGRKIYDHVFGSNIEAALSNEETWknrrrprpiyskdvlpesltqqngstQN 282
           ****99988887777666444.1...............122233455599*************************************99999999999 PP
   S: 277  csnaealqvpekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiepkldddlikeiagniipai 369
           cs+++   + + + +   ++ ++ +     + ++++al++f +k++++ g+l+f+kdd+ av++vtaaan+raes+gi+  ++ +  k+iagni++a+
   Q: 283  CSVTDGDLMVSAMPSLGLKNPQELWgltqnSLVFIEALKLFFAKRKKEIGHLTFDKDDQLAVEFVTAAANIRAESFGIPL-HSLFEAKGIAGNIVHAV 379
           99999999888888887777777778888999************************************************.***************** PP
   S: 370  attnavvgGlaaqEviKvvsgkfeplknff 399
           attna+++Gl+++E+iKv++++ ++++ ++
   Q: 380  ATTNAIIAGLIVIEAIKVLKKDVDKFRMTY 409
           **********************99999554 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

The dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.

Protein Sequence
(Fasta)

MATQQQQSAI KGAKVLMVGA GGIGCELLKT LALSGFEDIH IIDMDTIEVS NLNRQFLFRR 60
SHVGQSKAKV ARDAVLRFRP NINIRSYHAN VKNPEFDVDF FKQFDVVLNG LDNLDARRHV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GAAAATCAAA ATTCGCTACT ATTTCTCTGT TCCCTAATTT CTTTCGATTT CAATTCATTA 60
GTCCTAAAAA GCTTTAATTT TTCTAATCCC CCAGTTCTCC TTCTTCTGCT TCTGAATCGT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway
KW-0862--Zinc

Interpro

IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR028077--UAE_UbL_dom
IPR019572--UBA_E1_Cys

Pfam

PF00899--ThiF
PF14732--UAE_UbL
PF10585--UBA_e1_thiolCys

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0009506--C:plasmodesma
GO:0005524--F:ATP binding
GO:0046872--F:metal ion binding
GO:0019948--F:SUMO activating enzyme activity
GO:0009793--P:embryo development ending in seed dormancy
GO:0016925--P:protein sumoylation

KEGG

ath:AT2G21470

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000112	Aegilops tauschii	75.97	0.00e+00	826.00
IUUC-Aml-001662	Ailuropoda melanoleuca	41.21	2.00e-106	379.00
IUUC-Atr-002636	Amborella trichopoda	74.95	0.00e+00	858.00
IUUC-Apl-003369	Anas platyrhynchos	39.61	2.00e-93	335.00
IUUC-Aca-004490	Anolis carolinensis	41.92	3.00e-89	322.00
IUUC-Aly-006359	Arabidopsis lyrata	92.57	0.00e+00	1258.00
IUUC-Ago-007844	Ashbya gossypii	36.01	1.00e-72	266.00
IUUC-Acl-008270	Aspergillus clavatus	41.49	1.00e-106	380.00
IUUC-Afl-008674	Aspergillus flavus	42.16	2.00e-111	395.00
IUUC-Afu-008883	Aspergillus fumigatus	39.96	4.00e-105	374.00
IUUC-Ani-009500	Aspergillus nidulans	41.45	7.00e-109	387.00
IUUC-Ang-009535	Aspergillus niger	41.49	4.00e-105	374.00
IUUC-Aor-010223	Aspergillus oryzae	42.64	1.00e-110	392.00
IUUC-Ate-010282	Aspergillus terreus	43.75	3.00e-93	335.00
IUUC-Ame-011736	Astyanax mexicanus	40.85	3.00e-113	401.00
IUUC-Bgr-012166	Blumeria graminis	40.15	4.00e-98	351.00
IUUC-Bta-012589	Bos taurus	41.13	4.00e-107	381.00
IUUC-Bci-013802	Botrytis cinerea	41.89	2.00e-103	369.00
IUUC-Bdi-014697	Brachypodium distachyon	75.00	0.00e+00	827.00
IUUC-Bol-015899	Brassica oleracea	75.91	0.00e+00	1047.00
IUUC-Bra-018066	Brassica rapa	93.32	0.00e+00	1005.00
IUUC-Cel-018708	Caenorhabditis elegans	35.90	5.00e-93	334.00
IUUC-Cja-019120	Callithrix jacchus	41.51	9.00e-106	377.00
IUUC-Cfa-021061	Canis familiaris	41.32	2.00e-107	382.00
IUUC-Cpo-021842	Cavia porcellus	41.51	8.00e-106	377.00
IUUC-Csa-024104	Chlorocebus sabaeus	41.51	6.00e-106	377.00
IUUC-Cho-024332	Choloepus hoffmanni	37.71	7.00e-89	320.00
IUUC-Cin-025363	Ciona intestinalis	39.38	1.00e-103	369.00
IUUC-Cgl-026419	Colletotrichum gloeosporioides	40.65	1.00e-98	353.00
IUUC-Cne-026888	Cryptococcus neoformans	37.80	2.00e-76	279.00
IUUC-Cme-027315	Cyanidioschyzon merolae	33.13	2.00e-57	216.00
IUUC-Dre-028420	Danio rerio	40.00	4.00e-111	394.00
IUUC-Dno-030041	Dasypus novemcinctus	41.97	3.00e-106	378.00
IUUC-Dse-031504	Dothistroma septosporum	34.86	4.00e-84	305.00
IUUC-Dme-031771	Drosophila melanogaster	37.94	3.00e-81	295.00
IUUC-Ete-033037	Echinops telfairi	38.94	9.00e-89	320.00
IUUC-Eca-033774	Equus caballus	40.55	3.00e-100	358.00
IUUC-Eeu-035074	Erinaceus europaeus	34.73	5.00e-55	208.00
IUUC-Fca-036508	Felis catus	40.74	8.00e-101	360.00
IUUC-Fal-036813	Ficedula albicollis	39.80	4.00e-97	348.00
IUUC-Fox-038056	Fusarium oxysporum	41.62	6.00e-103	367.00
IUUC-Fso-038223	Fusarium solani	40.51	9.00e-99	353.00
IUUC-Gmo-038526	Gadus morhua	41.83	4.00e-97	348.00
IUUC-Ggr-039767	Gaeumannomyces graminis	40.62	2.00e-95	342.00
IUUC-Gga-040981	Gallus gallus	41.44	9.00e-97	347.00
IUUC-Gac-041437	Gasterosteus aculeatus	41.67	3.00e-110	391.00
IUUC-Gma-043041	Glycine max	79.20	0.00e+00	880.00
IUUC-Ggo-045358	Gorilla gorilla	43.53	9.00e-103	367.00
IUUC-Hsa-046068	Homo sapiens	41.51	8.00e-106	377.00
IUUC-Hvu-047646	Hordeum vulgare	75.15	0.00e+00	822.00
IUUC-Itr-047887	Ictidomys tridecemlineatus	41.97	4.00e-106	378.00
IUUC-Lch-049565	Latimeria chalumnae	41.91	1.00e-112	399.00
IUUC-Lpe-050884	Leersia perrieri	71.35	0.00e+00	838.00
IUUC-Loc-052493	Lepisosteus oculatus	41.58	3.00e-113	401.00
IUUC-Lma-053117	Leptosphaeria maculans	40.25	4.00e-102	364.00
IUUC-Laf-054518	Loxodonta africana	41.94	2.00e-106	379.00
IUUC-Mcc-055334	Macaca mulatta	41.32	1.00e-105	376.00
IUUC-Mor-057137	Magnaporthe oryzae	41.75	3.00e-94	338.00
IUUC-Mpo-057449	Magnaporthe poae	40.68	4.00e-97	348.00
IUUC-Mtr-058045	Medicago truncatula	78.44	0.00e+00	880.00
IUUC-Mla-058864	Melampsora laricipopulina	42.58	1.00e-82	300.00
IUUC-Mga-059422	Meleagris gallopavo	38.45	1.00e-80	293.00
IUUC-Mvi-060536	Microbotryum violaceum	38.59	5.00e-84	305.00
IUUC-Mmr-061161	Microcebus murinus	41.04	1.00e-103	369.00
IUUC-Mdo-061802	Monodelphis domestica	41.89	3.00e-106	378.00
IUUC-Mmu-064357	Mus musculus	42.20	7.00e-112	397.00
IUUC-Mac-065475	Musa acuminata	75.92	0.00e+00	832.00
IUUC-Mpu-066895	Mustela putorius furo	41.32	3.00e-107	382.00
IUUC-Mlu-067862	Myotis lucifugus	41.35	2.00e-107	382.00
IUUC-Nfi-068385	Neosartorya fischeri	42.13	2.00e-110	392.00
IUUC-Ncr-068885	Neurospora crassa	40.65	3.00e-97	348.00
IUUC-Nle-069524	Nomascus leucogenys	40.94	1.00e-103	369.00
IUUC-Opr-071038	Ochotona princeps	42.94	6.00e-114	404.00
IUUC-Ont-071719	Oreochromis niloticus	41.65	6.00e-113	400.00
IUUC-Oan-072877	Ornithorhynchus anatinus	40.89	2.00e-96	345.00
IUUC-Ocu-074080	Oryctolagus cuniculus	41.97	5.00e-106	378.00
IUUC-Oba-075936	Oryza barthii	76.55	0.00e+00	832.00
IUUC-Obr-076841	Oryza brachyantha	77.13	0.00e+00	840.00
IUUC-Ogl-077686	Oryza glaberrima	76.10	0.00e+00	841.00
IUUC-Ogu-078597	Oryza glumaepatula	74.39	0.00e+00	823.00
IUUC-Oin-079791	Oryza indica	76.55	0.00e+00	832.00
IUUC-Olo-081009	Oryza longistaminata	76.55	0.00e+00	833.00
IUUC-Ome-081327	Oryza meridionalis	70.36	0.00e+00	809.00
IUUC-Oni-082334	Oryza nivara	74.81	0.00e+00	825.00
IUUC-Opu-083447	Oryza punctata	76.16	0.00e+00	828.00
IUUC-Oru-084977	Oryza rufipogon	76.55	0.00e+00	832.00
IUUC-Osa-086160	Oryza sativa	80.05	0.00e+00	655.00
IUUC-Ola-087415	Oryzias latipes	40.63	3.00e-108	385.00
IUUC-Olu-087850	Ostreococcus lucimarinus	49.24	3.00e-146	510.00
IUUC-Oga-088434	Otolemur garnettii	41.97	2.00e-106	379.00
IUUC-Oar-089872	Ovis aries	40.35	9.00e-101	360.00
IUUC-Ptr-091212	Pan troglodytes	41.51	8.00e-106	377.00
IUUC-Pan-092708	Papio anubis	59.78	3.00e-65	241.00
IUUC-Psi-093703	Pelodiscus sinensis	40.89	6.00e-100	357.00
IUUC-Pno-094953	Phaeosphaeria nodorum	41.63	1.00e-105	376.00
IUUC-Ppa-095963	Physcomitrella patens	62.80	0.00e+00	685.00
IUUC-Pfo-097515	Poecilia formosa	40.55	3.00e-108	385.00
IUUC-Pab-097951	Pongo abelii	38.74	2.00e-79	288.00
IUUC-Pop-099703	Populus trichocarpa	75.33	0.00e+00	815.00
IUUC-Pca-100426	Procavia capensis	40.99	3.00e-105	375.00
IUUC-Ppe-101152	Prunus persica	79.28	0.00e+00	904.00
IUUC-Pva-102792	Pteropus vampyrus	39.28	1.00e-96	346.00
IUUC-Pgr-103592	Puccinia graminis	42.09	4.00e-82	298.00
IUUC-Ptt-103805	Puccinia triticina	35.70	7.00e-68	251.00
IUUC-Pte-104215	Pyrenophora teres	39.09	5.00e-110	390.00
IUUC-Pyt-104634	Pyrenophora triticirepentis	39.64	9.00e-112	396.00
IUUC-Rno-106117	Rattus norvegicus	42.02	3.00e-111	395.00
IUUC-Sce-106177	Saccharomyces cerevisiae	38.13	3.00e-67	248.00
IUUC-Sha-107006	Sarcophilus harrisii	42.45	4.00e-107	381.00
IUUC-Sja-107967	Schizosaccharomyces japonicus	40.27	1.00e-95	343.00
IUUC-Spo-108020	Schizosaccharomyces pombe	43.28	3.00e-100	358.00
IUUC-Ssl-108509	Sclerotinia sclerotiorum	41.85	1.00e-103	369.00
IUUC-Smo-108809	Selaginella moellendorffii	64.04	0.00e+00	675.00
IUUC-Sit-110581	Setaria italica	74.52	0.00e+00	818.00
IUUC-Sly-111870	Solanum lycopersicum	80.79	0.00e+00	899.00
IUUC-Stu-112283	Solanum tuberosum	80.04	0.00e+00	889.00
IUUC-Sar-113432	Sorex araneus	60.31	1.00e-45	177.00
IUUC-Sbi-114075	Sorghum bicolor	76.36	0.00e+00	838.00
IUUC-Sre-114926	Sporisorium reilianum	36.89	2.00e-90	325.00
IUUC-Ssc-116281	Sus scrofa	56.85	4.00e-66	243.00
IUUC-Tgu-117247	Taeniopygia guttata	39.80	8.00e-97	347.00
IUUC-Tru-118075	Takifugu rubripes	41.50	2.00e-101	362.00
IUUC-Tsy-119398	Tarsius syrichta	38.36	3.00e-77	282.00
IUUC-Tni-120771	Tetraodon nigroviridis	38.57	1.00e-84	306.00
IUUC-Tca-121651	Theobroma cacao	81.54	0.00e+00	904.00
IUUC-Tre-122224	Trichoderma reesei	41.73	2.00e-98	353.00
IUUC-Tvi-122634	Trichoderma virens	39.96	2.00e-93	336.00
IUUC-Tae-125665	Triticum aestivum	75.82	0.00e+00	827.00
IUUC-Tur-126417	Triticum urartu	74.90	0.00e+00	825.00
IUUC-Tme-127016	Tuber melanosporum	42.11	1.00e-97	349.00
IUUC-Tbe-127678	Tupaia belangeri	64.17	5.00e-45	175.00
IUUC-Ttr-128598	Tursiops truncatus	38.68	1.00e-95	343.00
IUUC-Uma-129612	Ustilago maydis	37.18	5.00e-81	295.00
IUUC-Vda-129811	Verticillium dahliae	40.39	2.00e-92	332.00
IUUC-Vpa-130732	Vicugna pacos	30.80	4.00e-63	235.00
IUUC-Vvi-130832	Vitis vinifera	80.58	0.00e+00	870.00
IUUC-Xtr-132823	Xenopus tropicalis	42.00	5.00e-96	344.00
IUUC-Xma-133552	Xiphophorus maculatus	40.55	3.00e-108	385.00
IUUC-Yli-134666	Yarrowia lipolytica	36.99	3.00e-79	288.00
IUUC-Zma-134976	Zea mays	76.60	0.00e+00	833.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence