iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-064372

UUCD1 version

UUC-MuM-01111

Ensembl Protein ID

ENSMUSP00000054303.5

UniProt Accession

Q9CY34; Q99JK3; Q9D8K0; Q9D8K7; UB2FA_MOUSE

Genbank Protein ID

BAB25362.1; BAB25372.1; BAB27290.1; BAC27036.1; BAE32030.1; AAH06057.1; AAH16117.1

Protein Name

NEDD8-conjugating enzyme UBE2F; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; RING-type E3 NEDD8 transferase UBE2F; Ubiquitin-conjugating enzyme E2 F

Genbank Nucleotide ID

AK007937; AK007960; AK010957; AK030594; AK153481; BC006057; BC016117

Gene Name

Ube2f; Ube2fa

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000034343.14	ENSMUST00000059743.11	ENSMUSP00000054303.5

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
microRNA	TRANSFAC	RepTar	RAID2
Protein-protein Interaction
IID
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	1.40e-36	126.4	37	177

Active Site

Position(s)	Description	Evidence
116	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 2    lkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilk... 87
           l ke++ele++ p ++++++ d ++l+ +++++  p++ +Y+gg+F++e e+p+ Y++ PPkvk+ltki+hPn++e+G++Cls+l+   
   Q: 37   LVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVS-PDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLRehs 124
           679********************99********9.**************************************************7778 PP
   S: 88   .eeekWspalsvesvllsiqsllae.pnpesplneeaaellkknreeykkkvr 138
            + + W p+ ++++v+++++sl+++  n+++pln eaae++ +++e+++ kv+
   Q: 125  iDGTGWAPTRTLKDVVWGLNSLFTDlLNFDDPLNIEAAEHHLRDKEDFRDKVD 177
           8899*******************8879**********************9996 PP

Organism

Mus musculus

Functional Description
(View)

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Protein Sequence
(Fasta)

MLTLASKLKR DDGLKGSRTS ASTSDSTRRV SVRDKLLVKE VAELEANLPC TCKVHFPDPN 60
KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AGTCCCCGCC CCGCCACTTC CGGTCTCGCC GCCGGGAGCC GGTGCGGCTG TGAAGGCCAG 60
CGCGACTCAG TGCCGCTGCT CCGCCGGGCA TGGTTTTGGG TGCCGGCTCG CCTCGCCTGA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005524--F:ATP binding
GO:0019788--F:NEDD8 transferase activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0045116--P:protein neddylation

KEGG

mmu:67921

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001473	Ailuropoda melanoleuca	95.14	3.00e-92	330.00
IUUC-Apl-003569	Anas platyrhynchos	85.71	3.00e-48	182.00
IUUC-Aca-005152	Anolis carolinensis	91.35	7.00e-99	352.00
IUUC-Ame-011839	Astyanax mexicanus	79.46	1.00e-88	318.00
IUUC-Bta-013524	Bos taurus	95.14	1.00e-92	331.00
IUUC-Cel-018579	Caenorhabditis elegans	57.81	2.00e-35	141.00
IUUC-Cja-019211	Callithrix jacchus	97.30	7.00e-96	342.00
IUUC-Cfa-020014	Canis familiaris	95.14	3.00e-92	330.00
IUUC-Cpo-022479	Cavia porcellus	96.76	7.00e-96	342.00
IUUC-Cho-024583	Choloepus hoffmanni	64.86	9.00e-61	226.00
IUUC-Cin-025378	Ciona intestinalis	48.91	1.00e-52	198.00
IUUC-Csv-026195	Ciona savignyi	51.37	1.00e-50	192.00
IUUC-Dre-028671	Danio rerio	81.62	2.00e-90	324.00
IUUC-Dno-029004	Dasypus novemcinctus	95.68	9.00e-106	375.00
IUUC-Dor-030940	Dipodomys ordii	95.68	3.00e-95	340.00
IUUC-Eca-034345	Equus caballus	95.68	1.00e-92	331.00
IUUC-Fca-036125	Felis catus	95.14	4.00e-92	330.00
IUUC-Fal-036808	Ficedula albicollis	91.35	1.00e-100	358.00
IUUC-Gmo-039394	Gadus morhua	88.50	7.00e-60	221.00
IUUC-Gga-041269	Gallus gallus	87.10	3.00e-93	333.00
IUUC-Gac-041480	Gasterosteus aculeatus	82.16	9.00e-89	318.00
IUUC-Ggo-045010	Gorilla gorilla	96.76	2.00e-106	377.00
IUUC-Hsa-046664	Homo sapiens	96.76	2.00e-106	377.00
IUUC-Itr-048522	Ictidomys tridecemlineatus	96.76	1.00e-94	338.00
IUUC-Loc-052137	Lepisosteus oculatus	82.70	3.00e-91	327.00
IUUC-Laf-053694	Loxodonta africana	94.59	7.00e-97	345.00
IUUC-Mcc-055638	Macaca mulatta	96.76	2.00e-106	377.00
IUUC-Meu-056568	Macropus eugenii	77.30	5.00e-80	289.00
IUUC-Mga-059437	Meleagris gallopavo	92.43	1.00e-100	358.00
IUUC-Mmr-060651	Microcebus murinus	93.33	6.00e-90	323.00
IUUC-Mdo-061993	Monodelphis domestica	91.89	4.00e-100	357.00
IUUC-Mpu-066423	Mustela putorius furo	94.59	6.00e-92	329.00
IUUC-Mlu-067910	Myotis lucifugus	95.68	1.00e-92	332.00
IUUC-Nle-069032	Nomascus leucogenys	96.22	7.00e-96	342.00
IUUC-Opr-070798	Ochotona princeps	96.76	5.00e-106	376.00
IUUC-Ont-071232	Oreochromis niloticus	82.16	3.00e-89	320.00
IUUC-Oan-072779	Ornithorhynchus anatinus	90.91	6.00e-65	238.00
IUUC-Ocu-074366	Oryctolagus cuniculus	91.89	2.00e-102	363.00
IUUC-Ola-087548	Oryzias latipes	88.14	2.00e-61	227.00
IUUC-Oga-089149	Otolemur garnettii	97.30	5.00e-98	349.00
IUUC-Oar-089662	Ovis aries	95.14	1.00e-92	331.00
IUUC-Ptr-090895	Pan troglodytes	96.76	2.00e-106	377.00
IUUC-Pan-092771	Papio anubis	96.24	3.00e-105	373.00
IUUC-Psi-093582	Pelodiscus sinensis	91.35	2.00e-100	357.00
IUUC-Pma-094321	Petromyzon marinus	84.21	5.00e-67	245.00
IUUC-Pfo-096040	Poecilia formosa	83.24	2.00e-90	324.00
IUUC-Pab-097931	Pongo abelii	96.76	2.00e-106	377.00
IUUC-Pca-100697	Procavia capensis	94.48	7.00e-83	298.00
IUUC-Pva-102609	Pteropus vampyrus	94.59	3.00e-93	333.00
IUUC-Rno-105622	Rattus norvegicus	99.46	4.00e-97	346.00
IUUC-Sha-107614	Sarcophilus harrisii	91.40	2.00e-99	354.00
IUUC-Ssc-115597	Sus scrofa	94.05	1.00e-94	338.00
IUUC-Tgu-117397	Taeniopygia guttata	91.35	9.00e-101	358.00
IUUC-Tru-118496	Takifugu rubripes	80.54	5.00e-87	313.00
IUUC-Tsy-119592	Tarsius syrichta	96.76	1.00e-97	348.00
IUUC-Tni-119759	Tetraodon nigroviridis	81.08	1.00e-87	315.00
IUUC-Tbe-128085	Tupaia belangeri	79.26	2.00e-60	223.00
IUUC-Ttr-129228	Tursiops truncatus	94.05	1.00e-91	328.00
IUUC-Xtr-131773	Xenopus tropicalis	91.97	3.00e-62	229.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence