iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Ago-007855

Ensembl Protein ID

AAS53196

UniProt Accession

Q755K1; ATG3_ASHGO

Genbank Protein ID

AAS53196.2

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme ATG3

Genbank Nucleotide ID

AE016819

Gene Name

ATG3; AFL178W

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
AGOS_AFL178W	AAS53196	AAS53196

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	29.58	6.00e-30	128.00	IUUC-Ath-006802
E2/Autophagy_C	37.91	8.00e-22	101.00	IUUC-Hsa-046449
E2/Autophagy_C	56.13	1.00e-97	350.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
209	Glycyl thioester intermediate	{ECO:0000250}

Organism

Ashbya gossypii

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MLRSTLSNWR EYLTPVTHQS TFENTGQITP EEFIKAGDYL CHMFPTWRWN QQQGGMVYRD 60
FLPQDRQFLV TRKVPSNMRA ADSVNVGGEE ETSAGEYWVL QPQQESADGG EEIDIDEMLQ 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCTACGGA GTACACTCTC GAACTGGAGG GAGTATCTAA CGCCTGTTAC CCACCAGTCG 60
ACGTTCGAAA ACACAGGGCA GATCACGCCG GAAGAGTTCA TCAAGGCGGG CGACTATCTG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0032258--P:CVT pathway
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0044804--P:nucleophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

KEGG

ago:AGOS_AFL178W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	28.09	9.00e-25	105.00
IUUC-Aml-002081	Ailuropoda melanoleuca	37.91	1.00e-23	100.00
IUUC-Atr-002712	Amborella trichopoda	25.56	4.00e-18	82.80
IUUC-Apl-003956	Anas platyrhynchos	31.06	2.00e-13	67.40
IUUC-Aca-004578	Anolis carolinensis	37.09	2.00e-22	97.80
IUUC-Aly-005823	Arabidopsis lyrata	30.13	2.00e-31	127.00
IUUC-Ath-006802	Arabidopsis thaliana	29.58	1.00e-31	128.00
IUUC-Acl-008147	Aspergillus clavatus	35.00	4.00e-48	182.00
IUUC-Afl-008483	Aspergillus flavus	35.51	2.00e-51	193.00
IUUC-Afu-008818	Aspergillus fumigatus	36.90	1.00e-53	201.00
IUUC-Ani-009481	Aspergillus nidulans	41.08	8.00e-32	128.00
IUUC-Ang-009800	Aspergillus niger	37.99	6.00e-56	208.00
IUUC-Aor-010150	Aspergillus oryzae	35.51	2.00e-51	193.00
IUUC-Ate-010545	Aspergillus terreus	41.90	2.00e-32	130.00
IUUC-Ame-011793	Astyanax mexicanus	37.91	5.00e-24	102.00
IUUC-Bgr-012167	Blumeria graminis	32.58	8.00e-45	171.00
IUUC-Bta-012695	Bos taurus	37.91	1.00e-23	101.00
IUUC-Bci-013972	Botrytis cinerea	36.97	4.00e-24	103.00
IUUC-Bdi-014756	Brachypodium distachyon	28.93	1.00e-32	131.00
IUUC-Bol-015091	Brassica oleracea	29.30	2.00e-29	120.00
IUUC-Bra-016662	Brassica rapa	26.14	2.00e-25	107.00
IUUC-Cel-018143	Caenorhabditis elegans	32.03	3.00e-19	86.70
IUUC-Cja-018991	Callithrix jacchus	30.09	3.00e-33	132.00
IUUC-Cfa-020070	Canis familiaris	37.91	1.00e-23	101.00
IUUC-Cpo-021860	Cavia porcellus	37.91	1.00e-23	101.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	33.85	7.00e-39	152.00
IUUC-Csa-023881	Chlorocebus sabaeus	37.91	1.00e-23	101.00
IUUC-Cho-024852	Choloepus hoffmanni	37.91	1.00e-23	101.00
IUUC-Cin-025787	Ciona intestinalis	30.28	7.00e-38	148.00
IUUC-Csv-026369	Ciona savignyi	30.37	5.00e-36	142.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	34.99	2.00e-50	190.00
IUUC-Cne-026905	Cryptococcus neoformans	31.44	2.00e-19	88.20
IUUC-Dre-028811	Danio rerio	38.56	2.00e-24	103.00
IUUC-Dno-030081	Dasypus novemcinctus	37.91	2.00e-23	100.00
IUUC-Dor-030183	Dipodomys ordii	51.43	1.00e-16	78.60
IUUC-Dse-031539	Dothistroma septosporum	38.51	2.00e-19	87.40
IUUC-Dme-031805	Drosophila melanogaster	35.25	5.00e-23	99.40
IUUC-Ete-033171	Echinops telfairi	38.56	5.00e-24	102.00
IUUC-Eca-034083	Equus caballus	37.91	1.00e-23	101.00
IUUC-Eeu-034804	Erinaceus europaeus	37.25	3.00e-23	100.00
IUUC-Fca-036289	Felis catus	37.91	1.00e-23	101.00
IUUC-Fal-037060	Ficedula albicollis	37.91	2.00e-23	100.00
IUUC-Fox-038051	Fusarium oxysporum	34.67	1.00e-49	188.00
IUUC-Fso-038124	Fusarium solani	37.84	4.00e-22	96.30
IUUC-Gmo-039538	Gadus morhua	33.94	8.00e-43	164.00
IUUC-Ggr-039880	Gaeumannomyces graminis	45.78	3.00e-36	143.00
IUUC-Gga-041108	Gallus gallus	37.91	2.00e-23	100.00
IUUC-Gac-041558	Gasterosteus aculeatus	32.92	4.00e-40	155.00
IUUC-Gma-043969	Glycine max	29.13	3.00e-27	113.00
IUUC-Ggo-045071	Gorilla gorilla	37.91	1.00e-23	101.00
IUUC-Hsa-046449	Homo sapiens	37.91	1.00e-23	101.00
IUUC-Hvu-047451	Hordeum vulgare	29.68	2.00e-32	130.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	37.91	1.00e-23	101.00
IUUC-Kpa-049096	Komagataella pastoris	48.28	3.00e-76	276.00
IUUC-Lch-049726	Latimeria chalumnae	31.44	3.00e-39	152.00
IUUC-Lpe-050734	Leersia perrieri	29.66	3.00e-26	110.00
IUUC-Loc-051983	Lepisosteus oculatus	34.34	1.00e-23	101.00
IUUC-Lma-053038	Leptosphaeria maculans	34.49	8.00e-52	195.00
IUUC-Laf-054510	Loxodonta africana	30.70	2.00e-33	133.00
IUUC-Mcc-054792	Macaca mulatta	37.91	1.00e-23	101.00
IUUC-Meu-055965	Macropus eugenii	31.93	2.00e-19	87.40
IUUC-Mor-057292	Magnaporthe oryzae	35.82	4.00e-49	186.00
IUUC-Mtr-058282	Medicago truncatula	29.22	7.00e-31	125.00
IUUC-Mla-059144	Melampsora laricipopulina	34.02	2.00e-23	100.00
IUUC-Mga-059509	Meleagris gallopavo	37.91	2.00e-23	100.00
IUUC-Mvi-060281	Microbotryum violaceum	32.98	2.00e-19	87.80
IUUC-Mmr-061788	Microcebus murinus	37.91	1.00e-23	101.00
IUUC-Mdo-062922	Monodelphis domestica	31.17	8.00e-38	148.00
IUUC-Mmu-064249	Mus musculus	37.91	1.00e-23	101.00
IUUC-Mac-064474	Musa acuminata	29.75	1.00e-31	127.00
IUUC-Mpu-065717	Mustela putorius furo	37.91	1.00e-23	101.00
IUUC-Mlu-067151	Myotis lucifugus	33.13	2.00e-20	90.50
IUUC-Nfi-068551	Neosartorya fischeri	36.06	3.00e-53	199.00
IUUC-Ncr-068846	Neurospora crassa	36.13	2.00e-53	200.00
IUUC-Nle-069790	Nomascus leucogenys	37.91	1.00e-23	101.00
IUUC-Opr-070828	Ochotona princeps	23.53	8.00e-04	34.70
IUUC-Ont-072517	Oreochromis niloticus	31.63	2.00e-39	154.00
IUUC-Oan-073285	Ornithorhynchus anatinus	34.94	7.00e-23	98.20
IUUC-Ocu-074695	Oryctolagus cuniculus	37.91	1.00e-23	101.00
IUUC-Oba-074991	Oryza barthii	31.80	2.00e-34	136.00
IUUC-Obr-076787	Oryza brachyantha	29.35	4.00e-33	132.00
IUUC-Ogl-077440	Oryza glaberrima	30.23	2.00e-33	134.00
IUUC-Ogu-078294	Oryza glumaepatula	30.55	4.00e-34	135.00
IUUC-Oin-079452	Oryza indica	30.65	4.00e-34	136.00
IUUC-Olo-080450	Oryza longistaminata	32.13	1.00e-34	137.00
IUUC-Ome-081514	Oryza meridionalis	30.55	5.00e-34	135.00
IUUC-Oni-082677	Oryza nivara	30.65	4.00e-34	136.00
IUUC-Opu-083925	Oryza punctata	30.23	2.00e-33	134.00
IUUC-Oru-084828	Oryza rufipogon	31.44	3.00e-35	139.00
IUUC-Osa-085937	Oryza sativa	30.65	4.00e-34	136.00
IUUC-Ola-086559	Oryzias latipes	29.68	2.00e-19	86.70
IUUC-Olu-087766	Ostreococcus lucimarinus	33.44	9.00e-39	151.00
IUUC-Oga-087983	Otolemur garnettii	37.91	1.00e-23	101.00
IUUC-Oar-089281	Ovis aries	37.91	1.00e-23	101.00
IUUC-Ptr-090983	Pan troglodytes	37.91	1.00e-23	101.00
IUUC-Pan-092457	Papio anubis	34.34	2.00e-23	100.00
IUUC-Psi-094046	Pelodiscus sinensis	37.91	2.00e-23	100.00
IUUC-Pma-094174	Petromyzon marinus	25.00	2.00e+00	24.60
IUUC-Pno-094925	Phaeosphaeria nodorum	35.16	1.00e-53	201.00
IUUC-Ppa-095487	Physcomitrella patens	28.57	3.00e-30	123.00
IUUC-Pfo-096968	Poecilia formosa	36.14	9.00e-25	105.00
IUUC-Pab-098621	Pongo abelii	37.91	2.00e-22	97.40
IUUC-Pop-100007	Populus trichocarpa	29.71	2.00e-30	123.00
IUUC-Pca-100590	Procavia capensis	37.91	2.00e-23	100.00
IUUC-Ppe-101657	Prunus persica	28.57	2.00e-27	114.00
IUUC-Pva-102446	Pteropus vampyrus	37.91	1.00e-23	101.00
IUUC-Pgr-103604	Puccinia graminis	45.78	6.00e-19	86.30
IUUC-Ptt-103941	Puccinia triticina	46.34	6.00e-19	86.30
IUUC-Pte-104252	Pyrenophora teres	34.86	2.00e-52	197.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	35.36	2.00e-52	197.00
IUUC-Rno-106025	Rattus norvegicus	37.91	1.00e-23	101.00
IUUC-Sce-106172	Saccharomyces cerevisiae	55.81	5.00e-93	331.00
IUUC-Sha-106858	Sarcophilus harrisii	31.27	2.00e-37	147.00
IUUC-Sja-107905	Schizosaccharomyces japonicus	33.12	1.00e-40	157.00
IUUC-Spo-108185	Schizosaccharomyces pombe	33.44	2.00e-37	147.00
IUUC-Smo-108883	Selaginella moellendorffii	28.74	2.00e-28	117.00
IUUC-Sit-110512	Setaria italica	28.85	7.00e-30	122.00
IUUC-Sly-111238	Solanum lycopersicum	27.58	1.00e-29	121.00
IUUC-Stu-112441	Solanum tuberosum	28.40	4.00e-30	122.00
IUUC-Sar-113039	Sorex araneus	34.34	2.00e-23	100.00
IUUC-Sbi-113776	Sorghum bicolor	30.16	2.00e-30	124.00
IUUC-Sre-115074	Sporisorium reilianum	46.91	1.00e-17	82.40
IUUC-Ssc-115262	Sus scrofa	33.33	8.00e-38	148.00
IUUC-Tgu-116790	Taeniopygia guttata	37.91	1.00e-23	101.00
IUUC-Tru-118160	Takifugu rubripes	31.64	1.00e-39	154.00
IUUC-Tsy-119145	Tarsius syrichta	28.10	4.00e-10	56.60
IUUC-Tni-119937	Tetraodon nigroviridis	30.75	3.00e-32	129.00
IUUC-Tca-121323	Theobroma cacao	28.80	6.00e-26	108.00
IUUC-Tre-121993	Trichoderma reesei	35.92	7.00e-51	192.00
IUUC-Tvi-122332	Trichoderma virens	37.72	1.00e-51	194.00
IUUC-Tae-124391	Triticum aestivum	31.63	2.00e-32	130.00
IUUC-Tur-126679	Triticum urartu	28.93	1.00e-31	128.00
IUUC-Tme-127159	Tuber melanosporum	34.52	8.00e-33	131.00
IUUC-Tbe-127590	Tupaia belangeri	22.83	2.00e-05	40.00
IUUC-Ttr-128261	Tursiops truncatus	37.91	8.00e-24	101.00
IUUC-Uma-129572	Ustilago maydis	32.65	2.00e-22	98.20
IUUC-Vda-129966	Verticillium dahliae	38.12	2.00e-54	204.00
IUUC-Vpa-130698	Vicugna pacos	37.91	1.00e-23	101.00
IUUC-Vvi-131240	Vitis vinifera	27.37	3.00e-06	43.10
IUUC-Xtr-132878	Xenopus tropicalis	31.25	2.00e-39	153.00
IUUC-Xma-134017	Xiphophorus maculatus	32.33	5.00e-40	155.00
IUUC-Yli-134373	Yarrowia lipolytica	48.79	3.00e-68	249.00
IUUC-Zma-135222	Zea mays	36.88	7.00e-23	99.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence