iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045881

UUCD1 version

UUC-HoS-01185

Ensembl Protein ID

ENSP00000316176.2

UniProt Accession

P61088; Q16781; Q53Y81; UBE2N_HUMAN

Genbank Protein ID

BAA11675.1; AAP35519.1; AAH00396.1; AAH03365.1; AAI08705.1

Protein Name

Ubiquitin-conjugating enzyme E2 N; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N

Genbank Nucleotide ID

D83004; BT006873; BC000396; BC003365; BC108704

Gene Name

UBE2N; BLU

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000177889.9	ENST00000318066.6	ENSP00000316176.2
ENSG00000177889.9	ENST00000550657.1	ENSP00000449352.1
ENSG00000177889.9	ENST00000549490.1	ENSP00000447009.1
ENSG00000177889.9	ENST00000552442.1	ENSP00000448352.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	CircNet	miRTarBase
microRNA	TRANSFAC	miRWalk	TargetScan
RepTar	miRNAMap	SomamiR	miRcode
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Drug and target
Manually
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	PHOSIDA	BioGRID
mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E2/UBC	E2_UBC	18001825; 20656690; 19648646; 17108083; 19465916; 19269966; 19264966; 18326498; 12591926; 20932476; 20146531

Classification

Family	E-value	Score	Start	End
E2/UBC	3.00e-47	161.5	7	139

Active Site

Position(s)	Description	Evidence
87	Glycyl thioester intermediate	N/A

Domain Profile

E2/UBC

   S: 1    RlkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilkeeekW 92
           R+ ke+++l ++p++gi+a+p+++ +   ++v+i Gp+d+p+egg+Fkle+ +pe+YP+ +Pkv+f+tki+hPnv++ G++Cl+ilk  +kW
   Q: 7    RIIKETQRLLAEPVPGIKAEPDES-NARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILK--DKW 95
           899*********************.9************************************************************9..*** PP
   S: 93   spalsvesvllsiqsllaepnpesplneeaaellkknreeykkk 136
           spal++++vllsiq+ll+ pnp++pl +++ae++k+n+++  ++
   Q: 96   SPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139
           *************************************9976655 PP

Organism

Homo sapiens

Functional Description
(View)

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

Protein Sequence
(Fasta)

MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE 60
EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGTTAAGAGA GACGCGCGCG CAGTCGCGCG CGGGTCGTGC CGTACCACCG TCGCGGGCAG 60
GCTCGGCCAC GAGCGCCAGA GCCCCGCGCC TCCCCTCGCG GCCTGTCCCA AGTCCCTGCC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-1015--Disulfide bond
KW-0227--DNA damage
KW-0234--DNA repair
KW-1017--Isopeptide bond
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0070062--C:extracellular exosome
GO:0001650--C:fibrillar center
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0043234--C:protein complex
GO:0031372--C:UBC13-MMS2 complex
GO:0035370--C:UBC13-UEV1A complex
GO:0000151--C:ubiquitin ligase complex
GO:0005524--F:ATP binding
GO:0003723--F:RNA binding
GO:0043130--F:ubiquitin binding
GO:0061631--F:ubiquitin conjugating enzyme activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0000187--P:activation of MAPK activity
GO:0006464--P:cellular protein modification process
GO:0000729--P:DNA double-strand break processing
GO:0000724--P:double-strand break repair via homologous recombination
GO:0006303--P:double-strand break repair via nonhomologous end joining
GO:0038095--P:Fc-epsilon receptor signaling pathway
GO:0070911--P:global genome nucleotide-excision repair
GO:0016574--P:histone ubiquitination
GO:0007254--P:JNK cascade
GO:0070423--P:nucleotide-binding oligomerization domain containing signaling pathway
GO:0045739--P:positive regulation of DNA repair
GO:0031058--P:positive regulation of histone modification
GO:0043123--P:positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0051092--P:positive regulation of NF-kappaB transcription factor activity
GO:0051443--P:positive regulation of ubiquitin-protein transferase activity
GO:0006301--P:postreplication repair
GO:0070534--P:protein K63-linked ubiquitination
GO:0016567--P:protein ubiquitination
GO:0006508--P:proteolysis
GO:0006282--P:regulation of DNA repair
GO:0033182--P:regulation of histone ubiquitination
GO:0002223--P:stimulatory C-type lectin receptor signaling pathway
GO:0050852--P:T cell receptor signaling pathway
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:7334

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000100	Aegilops tauschii	79.59	3.00e-69	253.00
IUUC-Aml-001889	Ailuropoda melanoleuca	97.24	5.00e-81	292.00
IUUC-Atr-002818	Amborella trichopoda	79.59	1.00e-69	255.00
IUUC-Apl-003469	Anas platyrhynchos	97.95	3.00e-83	299.00
IUUC-Aca-005051	Anolis carolinensis	100.00	2.00e-87	314.00
IUUC-Aly-006258	Arabidopsis lyrata	80.27	2.00e-70	257.00
IUUC-Ath-007639	Arabidopsis thaliana	79.59	7.00e-70	255.00
IUUC-Ago-007837	Ashbya gossypii	75.20	3.00e-54	204.00
IUUC-Acl-008263	Aspergillus clavatus	71.62	1.00e-63	234.00
IUUC-Afl-008733	Aspergillus flavus	72.30	7.00e-64	235.00
IUUC-Afu-008946	Aspergillus fumigatus	71.62	1.00e-63	234.00
IUUC-Ani-009482	Aspergillus nidulans	71.33	5.00e-64	236.00
IUUC-Ang-009678	Aspergillus niger	72.30	1.00e-63	234.00
IUUC-Aor-010025	Aspergillus oryzae	72.30	7.00e-64	235.00
IUUC-Ate-010254	Aspergillus terreus	71.23	2.00e-62	231.00
IUUC-Ame-010742	Astyanax mexicanus	98.03	1.00e-85	308.00
IUUC-Bgr-012156	Blumeria graminis	65.56	2.00e-54	204.00
IUUC-Bta-012636	Bos taurus	100.00	2.00e-87	314.00
IUUC-Bci-013761	Botrytis cinerea	72.48	5.00e-63	233.00
IUUC-Bdi-014796	Brachypodium distachyon	80.27	2.00e-70	257.00
IUUC-Bol-016613	Brassica oleracea	80.27	2.00e-70	257.00
IUUC-Bra-016938	Brassica rapa	80.27	2.00e-70	257.00
IUUC-Cel-018119	Caenorhabditis elegans	88.59	6.00e-75	272.00
IUUC-Cja-019108	Callithrix jacchus	100.00	2.00e-87	314.00
IUUC-Cfa-020561	Canis familiaris	99.34	8.00e-87	311.00
IUUC-Cpo-022280	Cavia porcellus	96.69	1.00e-83	301.00
IUUC-Cre-022978	Chlamydomonas reinhardtii	78.91	5.00e-67	246.00
IUUC-Csa-023860	Chlorocebus sabaeus	92.76	2.00e-80	290.00
IUUC-Cho-024459	Choloepus hoffmanni	100.00	2.00e-87	314.00
IUUC-Cin-025278	Ciona intestinalis	80.27	9.00e-69	252.00
IUUC-Csv-025952	Ciona savignyi	72.97	3.00e-60	223.00
IUUC-Cgl-026752	Colletotrichum gloeosporioides	71.43	2.00e-61	227.00
IUUC-Cne-026809	Cryptococcus neoformans	74.44	7.00e-57	212.00
IUUC-Cme-027100	Cyanidioschyzon merolae	65.77	2.00e-56	211.00
IUUC-Dre-027814	Danio rerio	98.03	1.00e-85	308.00
IUUC-Dno-030059	Dasypus novemcinctus	98.03	1.00e-85	308.00
IUUC-Dor-030269	Dipodomys ordii	100.00	2.00e-73	266.00
IUUC-Dse-031505	Dothistroma septosporum	68.03	1.00e-59	221.00
IUUC-Dme-032097	Drosophila melanogaster	80.13	2.00e-72	264.00
IUUC-Ete-032369	Echinops telfairi	100.00	2.00e-87	314.00
IUUC-Eca-033230	Equus caballus	100.00	2.00e-87	314.00
IUUC-Fca-036161	Felis catus	95.39	3.00e-83	300.00
IUUC-Fal-036781	Ficedula albicollis	96.71	9.00e-85	305.00
IUUC-Fox-037840	Fusarium oxysporum	71.92	1.00e-60	225.00
IUUC-Fso-038118	Fusarium solani	71.92	6.00e-61	226.00
IUUC-Gmo-039561	Gadus morhua	93.42	1.00e-82	298.00
IUUC-Ggr-039807	Gaeumannomyces graminis	72.79	2.00e-62	231.00
IUUC-Gga-041024	Gallus gallus	100.00	2.00e-87	314.00
IUUC-Gac-042450	Gasterosteus aculeatus	92.05	7.00e-81	292.00
IUUC-Gma-043982	Glycine max	80.27	2.00e-70	257.00
IUUC-Ggo-044660	Gorilla gorilla	99.34	9.00e-87	311.00
IUUC-Hvu-047346	Hordeum vulgare	80.27	2.00e-70	257.00
IUUC-Itr-048350	Ictidomys tridecemlineatus	92.11	2.00e-78	284.00
IUUC-Kpa-049285	Komagataella pastoris	70.27	8.00e-63	232.00
IUUC-Lch-050548	Latimeria chalumnae	93.71	7.00e-79	285.00
IUUC-Lpe-051394	Leersia perrieri	80.27	3.00e-70	256.00
IUUC-Loc-052205	Lepisosteus oculatus	98.03	1.00e-85	308.00
IUUC-Lma-053056	Leptosphaeria maculans	72.60	2.00e-62	231.00
IUUC-Laf-053540	Loxodonta africana	100.00	1.00e-81	294.00
IUUC-Mcc-054863	Macaca mulatta	100.00	2.00e-87	314.00
IUUC-Meu-056482	Macropus eugenii	100.00	1.00e-81	294.00
IUUC-Mor-057014	Magnaporthe oryzae	72.60	4.00e-61	226.00
IUUC-Mpo-057586	Magnaporthe poae	72.79	2.00e-62	231.00
IUUC-Mtr-058552	Medicago truncatula	72.05	2.00e-66	244.00
IUUC-Mla-058920	Melampsora laricipopulina	70.07	4.00e-61	226.00
IUUC-Mga-059649	Meleagris gallopavo	96.64	2.00e-82	297.00
IUUC-Mvi-060483	Microbotryum violaceum	75.51	6.00e-65	239.00
IUUC-Mmr-061167	Microcebus murinus	100.00	2.00e-87	314.00
IUUC-Mdo-062701	Monodelphis domestica	100.00	2.00e-87	314.00
IUUC-Mmu-064070	Mus musculus	100.00	2.00e-87	314.00
IUUC-Mac-064742	Musa acuminata	80.95	6.00e-71	259.00
IUUC-Mpu-066401	Mustela putorius furo	100.00	2.00e-87	313.00
IUUC-Mlu-067229	Myotis lucifugus	98.64	7.00e-83	298.00
IUUC-Nfi-068526	Neosartorya fischeri	71.62	1.00e-63	234.00
IUUC-Ncr-068643	Neurospora crassa	72.11	5.00e-62	229.00
IUUC-Nle-070146	Nomascus leucogenys	100.00	2.00e-87	314.00
IUUC-Opr-070232	Ochotona princeps	78.29	2.00e-63	234.00
IUUC-Ont-072369	Oreochromis niloticus	94.67	2.00e-82	297.00
IUUC-Oan-073174	Ornithorhynchus anatinus	96.05	2.00e-83	301.00
IUUC-Ocu-074712	Oryctolagus cuniculus	100.00	2.00e-87	314.00
IUUC-Oba-075408	Oryza barthii	80.27	3.00e-70	256.00
IUUC-Obr-076881	Oryza brachyantha	80.27	3.00e-70	256.00
IUUC-Ogl-077242	Oryza glaberrima	80.27	3.00e-70	256.00
IUUC-Ogu-078465	Oryza glumaepatula	80.27	3.00e-70	256.00
IUUC-Oin-080035	Oryza indica	80.43	6.00e-66	242.00
IUUC-Olo-080555	Oryza longistaminata	80.27	3.00e-70	256.00
IUUC-Ome-081653	Oryza meridionalis	80.27	3.00e-70	256.00
IUUC-Oni-082400	Oryza nivara	80.27	3.00e-70	256.00
IUUC-Opu-083130	Oryza punctata	80.43	3.00e-65	241.00
IUUC-Oru-084649	Oryza rufipogon	80.27	3.00e-70	256.00
IUUC-Osa-085629	Oryza sativa	80.27	3.00e-70	256.00
IUUC-Ola-087083	Oryzias latipes	94.67	3.00e-82	296.00
IUUC-Olu-087886	Ostreococcus lucimarinus	68.03	1.00e-58	218.00
IUUC-Oga-088983	Otolemur garnettii	96.00	5.00e-83	299.00
IUUC-Oar-090440	Ovis aries	99.34	7.00e-87	311.00
IUUC-Ptr-090572	Pan troglodytes	100.00	2.00e-87	314.00
IUUC-Pan-092503	Papio anubis	98.03	9.00e-86	308.00
IUUC-Psi-093419	Pelodiscus sinensis	100.00	4.00e-78	283.00
IUUC-Pma-094136	Petromyzon marinus	92.86	3.00e-43	165.00
IUUC-Pno-094838	Phaeosphaeria nodorum	72.30	2.00e-63	234.00
IUUC-Ppa-096014	Physcomitrella patens	82.19	8.00e-71	258.00
IUUC-Pfo-096074	Poecilia formosa	95.33	1.00e-82	298.00
IUUC-Pab-097778	Pongo abelii	100.00	2.00e-87	314.00
IUUC-Pop-099707	Populus trichocarpa	80.27	3.00e-70	256.00
IUUC-Pca-100334	Procavia capensis	83.55	6.00e-73	266.00
IUUC-Ppe-101149	Prunus persica	80.27	2.00e-70	257.00
IUUC-Pva-102979	Pteropus vampyrus	100.00	2.00e-87	314.00
IUUC-Pgr-103654	Puccinia graminis	70.75	2.00e-60	224.00
IUUC-Ptt-103752	Puccinia triticina	69.44	4.00e-58	216.00
IUUC-Pte-104137	Pyrenophora teres	71.23	1.00e-61	228.00
IUUC-Pyt-104513	Pyrenophora triticirepentis	71.23	1.00e-61	228.00
IUUC-Rno-105927	Rattus norvegicus	99.34	4.00e-87	312.00
IUUC-Sce-106178	Saccharomyces cerevisiae	70.27	1.00e-61	228.00
IUUC-Sha-107493	Sarcophilus harrisii	100.00	2.00e-87	314.00
IUUC-Sja-107878	Schizosaccharomyces japonicus	66.89	1.00e-57	216.00
IUUC-Spo-108062	Schizosaccharomyces pombe	68.71	9.00e-57	212.00
IUUC-Ssl-108395	Sclerotinia sclerotiorum	70.95	2.00e-61	227.00
IUUC-Smo-109587	Selaginella moellendorffii	74.67	9.00e-64	235.00
IUUC-Sit-110151	Setaria italica	80.27	4.00e-70	257.00
IUUC-Sly-111322	Solanum lycopersicum	80.27	2.00e-70	257.00
IUUC-Stu-112254	Solanum tuberosum	79.59	6.00e-70	256.00
IUUC-Sar-113464	Sorex araneus	100.00	1.00e-81	294.00
IUUC-Sbi-114864	Sorghum bicolor	80.27	9.00e-70	255.00
IUUC-Sre-114927	Sporisorium reilianum	78.08	8.00e-67	245.00
IUUC-Ssc-116096	Sus scrofa	100.00	2.00e-87	314.00
IUUC-Tgu-117266	Taeniopygia guttata	100.00	1.00e-81	294.00
IUUC-Tru-117903	Takifugu rubripes	94.04	1.00e-81	294.00
IUUC-Tsy-118952	Tarsius syrichta	100.00	1.00e-81	294.00
IUUC-Tni-120038	Tetraodon nigroviridis	94.04	1.00e-81	294.00
IUUC-Tca-121752	Theobroma cacao	80.58	8.00e-66	243.00
IUUC-Tre-121979	Trichoderma reesei	73.97	4.00e-62	229.00
IUUC-Tvi-122515	Trichoderma virens	73.29	2.00e-61	227.00
IUUC-Tae-125309	Triticum aestivum	80.27	2.00e-70	257.00
IUUC-Tur-126865	Triticum urartu	79.86	6.00e-65	239.00
IUUC-Tme-127129	Tuber melanosporum	73.97	6.00e-63	232.00
IUUC-Tbe-127846	Tupaia belangeri	100.00	1.00e-81	294.00
IUUC-Ttr-128808	Tursiops truncatus	96.71	7.00e-84	302.00
IUUC-Uma-129548	Ustilago maydis	78.77	2.00e-67	247.00
IUUC-Vda-129810	Verticillium dahliae	71.23	1.00e-60	225.00
IUUC-Vpa-130221	Vicugna pacos	91.37	3.00e-69	253.00
IUUC-Vvi-131416	Vitis vinifera	66.29	2.00e-65	241.00
IUUC-Xtr-132366	Xenopus tropicalis	96.75	2.00e-84	304.00
IUUC-Xma-132986	Xiphophorus maculatus	94.67	3.00e-82	296.00
IUUC-Yli-134605	Yarrowia lipolytica	72.97	1.00e-63	234.00
IUUC-Zma-135587	Zea mays	76.97	6.00e-68	249.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence