iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Bta-012818

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M

Genbank Nucleotide ID

BC133501

Gene Name

UBE2M; UBC12

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSBTAG00000012744.4	ENSBTAT00000016937.4	ENSBTAP00000016937.4

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	2.40e-39	134.4	33	165

Active Site

Position(s)	Description	Evidence
111	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 1    RlkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilkee 89
           R++k+++el  + p+++ ++++d +dl +++++i+ p++  Y+ g+F +++++ + YP +PPkvk++t ++hPn++ +G+vCl+il+  
   Q: 33   RIQKDINEL--NLPKTCDISFSDPDDLLNFKLVIC-PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILR-- 116
           89******9..89999********9*********9.**************************************************9.. PP
   S: 90   ekWspalsvesvllsiqsllaepnpesplneeaaellkknreeykkkvr 138
           e+W+p+l+++s+++++q l+ epnpe+pln+eaae l++nr+ ++++v+
   Q: 117  EDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165
           ***********************************************97 PP

Organism

Bos taurus

Functional Description
(View)

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (By similarity).

Protein Sequence
(Fasta)

MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF 60
KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGTGGGGCGC CCGAGGCCGT TGCGGACCTC CGCGCCGAAG CCGCTGCTGC CACGGAAGCC 60
GGCCCTCCTA TCTAGTACAC GGCCACCGCA GCCCCCGCCT CCCCGTTCCG CCTCGAGCCG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0488--Methylation
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0005524--F:ATP binding
GO:0019788--F:NEDD8 transferase activity
GO:0018169--F:ribosomal S6-glutamic acid ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006464--P:cellular protein modification process
GO:0045116--P:protein neddylation

KEGG

bta:613343

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000196	Aegilops tauschii	56.82	2.00e-54	204.00
IUUC-Aml-002007	Ailuropoda melanoleuca	100.00	5.00e-86	308.00
IUUC-Atr-003170	Amborella trichopoda	60.11	3.00e-65	240.00
IUUC-Aca-005037	Anolis carolinensis	97.81	2.00e-104	370.00
IUUC-Aly-006219	Arabidopsis lyrata	61.62	6.00e-66	243.00
IUUC-Ath-006846	Arabidopsis thaliana	62.16	2.00e-65	241.00
IUUC-Ago-007910	Ashbya gossypii	44.59	4.00e-36	144.00
IUUC-Acl-008364	Aspergillus clavatus	59.87	1.00e-54	205.00
IUUC-Afl-008463	Aspergillus flavus	62.50	1.00e-56	211.00
IUUC-Afu-008803	Aspergillus fumigatus	59.87	8.00e-55	206.00
IUUC-Ani-009422	Aspergillus nidulans	62.50	3.00e-57	214.00
IUUC-Ang-009728	Aspergillus niger	62.96	1.00e-50	191.00
IUUC-Aor-009962	Aspergillus oryzae	62.50	1.00e-56	211.00
IUUC-Ate-010430	Aspergillus terreus	59.01	4.00e-51	194.00
IUUC-Ame-011382	Astyanax mexicanus	90.27	8.00e-97	345.00
IUUC-Bgr-012306	Blumeria graminis	55.98	2.00e-60	224.00
IUUC-Bci-013626	Botrytis cinerea	56.52	6.00e-60	223.00
IUUC-Bdi-014528	Brachypodium distachyon	63.74	2.00e-63	234.00
IUUC-Bol-016135	Brassica oleracea	60.75	9.00e-66	242.00
IUUC-Bra-016704	Brassica rapa	60.54	2.00e-65	241.00
IUUC-Cja-019213	Callithrix jacchus	97.34	1.00e-104	371.00
IUUC-Cfa-020242	Canis familiaris	98.92	3.00e-105	373.00
IUUC-Cpo-022405	Cavia porcellus	95.63	2.00e-100	357.00
IUUC-Cre-022865	Chlamydomonas reinhardtii	60.57	8.00e-55	206.00
IUUC-Csa-023269	Chlorocebus sabaeus	100.00	6.00e-107	379.00
IUUC-Cin-025333	Ciona intestinalis	74.86	9.00e-82	295.00
IUUC-Csv-025898	Ciona savignyi	61.57	3.00e-74	270.00
IUUC-Cgl-026477	Colletotrichum gloeosporioides	56.28	4.00e-57	213.00
IUUC-Cne-027097	Cryptococcus neoformans	60.99	5.00e-62	229.00
IUUC-Cme-027287	Cyanidioschyzon merolae	48.05	1.00e-18	86.30
IUUC-Dre-028514	Danio rerio	87.03	5.00e-89	319.00
IUUC-Dno-029678	Dasypus novemcinctus	92.35	2.00e-97	347.00
IUUC-Dor-030851	Dipodomys ordii	67.66	5.00e-57	213.00
IUUC-Dse-031265	Dothistroma septosporum	56.83	2.00e-62	231.00
IUUC-Dme-032076	Drosophila melanogaster	75.27	3.00e-80	290.00
IUUC-Ete-032350	Echinops telfairi	73.04	8.00e-44	168.00
IUUC-Eca-034075	Equus caballus	98.91	2.00e-105	374.00
IUUC-Fca-035654	Felis catus	100.00	5.00e-86	308.00
IUUC-Fal-037283	Ficedula albicollis	87.95	6.00e-41	157.00
IUUC-Fox-037877	Fusarium oxysporum	57.30	2.00e-57	214.00
IUUC-Fso-038428	Fusarium solani	57.30	5.00e-63	233.00
IUUC-Gmo-039402	Gadus morhua	89.19	2.00e-96	343.00
IUUC-Ggr-039731	Gaeumannomyces graminis	59.24	8.00e-57	212.00
IUUC-Gga-041122	Gallus gallus	89.83	2.00e-92	331.00
IUUC-Gma-043805	Glycine max	60.54	9.00e-64	235.00
IUUC-Ggo-044436	Gorilla gorilla	100.00	6.00e-107	379.00
IUUC-Hsa-046026	Homo sapiens	100.00	6.00e-107	379.00
IUUC-Hvu-047056	Hordeum vulgare	60.99	3.00e-62	230.00
IUUC-Itr-047889	Ictidomys tridecemlineatus	100.00	6.00e-107	379.00
IUUC-Kpa-049315	Komagataella pastoris	49.16	7.00e-49	186.00
IUUC-Lch-050360	Latimeria chalumnae	75.61	3.00e-59	221.00
IUUC-Lpe-050743	Leersia perrieri	61.02	7.00e-63	233.00
IUUC-Lma-053001	Leptosphaeria maculans	57.92	4.00e-58	216.00
IUUC-Laf-053463	Loxodonta africana	99.32	2.00e-85	307.00
IUUC-Mcc-054966	Macaca mulatta	100.00	7.00e-44	167.00
IUUC-Meu-056085	Macropus eugenii	97.26	4.00e-83	299.00
IUUC-Mor-057250	Magnaporthe oryzae	61.84	2.00e-55	208.00
IUUC-Mpo-057481	Magnaporthe poae	63.16	3.00e-55	207.00
IUUC-Mtr-058251	Medicago truncatula	59.56	1.00e-64	238.00
IUUC-Mla-059119	Melampsora laricipopulina	59.02	7.00e-60	222.00
IUUC-Mga-059881	Meleagris gallopavo	90.57	3.00e-55	206.00
IUUC-Mvi-060449	Microbotryum violaceum	58.10	9.00e-59	219.00
IUUC-Mmr-061511	Microcebus murinus	100.00	4.00e-44	168.00
IUUC-Mmu-063962	Mus musculus	100.00	6.00e-107	379.00
IUUC-Mac-064967	Musa acuminata	62.84	7.00e-68	249.00
IUUC-Mlu-066955	Myotis lucifugus	98.01	2.00e-86	310.00
IUUC-Nfi-068300	Neosartorya fischeri	59.87	8.00e-55	206.00
IUUC-Ncr-068944	Neurospora crassa	56.76	6.00e-57	213.00
IUUC-Nle-069051	Nomascus leucogenys	100.00	5.00e-86	308.00
IUUC-Opr-070635	Ochotona princeps	100.00	2.00e-85	306.00
IUUC-Ont-072348	Oreochromis niloticus	89.19	4.00e-96	343.00
IUUC-Ocu-074905	Oryctolagus cuniculus	100.00	6.00e-107	379.00
IUUC-Oba-075825	Oryza barthii	61.54	6.00e-67	246.00
IUUC-Obr-076067	Oryza brachyantha	60.99	2.00e-66	244.00
IUUC-Ogl-077594	Oryza glaberrima	61.54	6.00e-67	246.00
IUUC-Ogu-078073	Oryza glumaepatula	61.54	6.00e-67	246.00
IUUC-Oin-080060	Oryza indica	61.20	1.00e-63	236.00
IUUC-Olo-080314	Oryza longistaminata	61.54	3.00e-67	247.00
IUUC-Ome-081713	Oryza meridionalis	61.54	7.00e-67	246.00
IUUC-Oni-082426	Oryza nivara	61.54	6.00e-67	246.00
IUUC-Opu-083942	Oryza punctata	61.85	4.00e-63	233.00
IUUC-Oru-085104	Oryza rufipogon	61.54	6.00e-67	246.00
IUUC-Osa-086168	Oryza sativa	61.54	6.00e-67	246.00
IUUC-Olu-087854	Ostreococcus lucimarinus	56.76	8.00e-58	216.00
IUUC-Oga-088580	Otolemur garnettii	100.00	6.00e-107	379.00
IUUC-Oar-089623	Ovis aries	93.24	3.00e-78	283.00
IUUC-Ptr-091161	Pan troglodytes	90.71	2.00e-93	333.00
IUUC-Pan-092119	Papio anubis	100.00	5.00e-86	308.00
IUUC-Psi-093494	Pelodiscus sinensis	97.28	2.00e-83	300.00
IUUC-Pno-094972	Phaeosphaeria nodorum	56.02	6.00e-56	209.00
IUUC-Ppa-095187	Physcomitrella patens	67.76	2.00e-70	257.00
IUUC-Pfo-097205	Poecilia formosa	85.56	2.00e-87	314.00
IUUC-Pab-098434	Pongo abelii	100.00	6.00e-107	379.00
IUUC-Pop-099789	Populus trichocarpa	61.20	2.00e-65	241.00
IUUC-Pca-100300	Procavia capensis	99.32	7.00e-85	305.00
IUUC-Ppe-101143	Prunus persica	60.66	2.00e-65	241.00
IUUC-Pva-102143	Pteropus vampyrus	100.00	6.00e-107	379.00
IUUC-Pgr-103675	Puccinia graminis	59.78	9.00e-59	219.00
IUUC-Ptt-103997	Puccinia triticina	61.24	6.00e-60	223.00
IUUC-Pte-104042	Pyrenophora teres	57.92	1.00e-57	215.00
IUUC-Pyt-104616	Pyrenophora triticirepentis	61.90	7.00e-53	199.00
IUUC-Rno-105107	Rattus norvegicus	100.00	6.00e-107	379.00
IUUC-Sce-106385	Saccharomyces cerevisiae	44.10	2.00e-37	148.00
IUUC-Sha-106899	Sarcophilus harrisii	90.18	1.00e-84	305.00
IUUC-Sja-107789	Schizosaccharomyces japonicus	51.10	3.00e-51	194.00
IUUC-Spo-108143	Schizosaccharomyces pombe	49.45	6.00e-50	189.00
IUUC-Ssl-108427	Sclerotinia sclerotiorum	56.28	2.00e-58	218.00
IUUC-Smo-108673	Selaginella moellendorffii	64.67	4.00e-68	250.00
IUUC-Sit-109702	Setaria italica	64.29	3.00e-62	230.00
IUUC-Sly-111650	Solanum lycopersicum	63.39	4.00e-67	246.00
IUUC-Stu-112655	Solanum tuberosum	62.84	2.00e-67	248.00
IUUC-Sar-113632	Sorex araneus	99.45	8.00e-106	375.00
IUUC-Sbi-114357	Sorghum bicolor	64.84	4.00e-63	233.00
IUUC-Sre-114912	Sporisorium reilianum	63.82	4.00e-58	216.00
IUUC-Tsy-119639	Tarsius syrichta	77.18	2.00e-60	224.00
IUUC-Tni-119674	Tetraodon nigroviridis	77.30	2.00e-77	280.00
IUUC-Tca-121359	Theobroma cacao	62.84	1.00e-66	245.00
IUUC-Tre-122115	Trichoderma reesei	59.75	1.00e-56	212.00
IUUC-Tvi-122656	Trichoderma virens	59.75	4.00e-56	210.00
IUUC-Tae-124489	Triticum aestivum	60.99	3.00e-62	230.00
IUUC-Tur-126125	Triticum urartu	60.99	3.00e-62	231.00
IUUC-Tme-126950	Tuber melanosporum	62.42	9.00e-58	215.00
IUUC-Tbe-127874	Tupaia belangeri	71.58	3.00e-66	244.00
IUUC-Ttr-128213	Tursiops truncatus	98.76	4.00e-91	326.00
IUUC-Uma-129329	Ustilago maydis	64.29	7.00e-58	216.00
IUUC-Vda-129754	Verticillium dahliae	61.78	3.00e-57	214.00
IUUC-Vvi-131182	Vitis vinifera	62.30	1.00e-65	242.00
IUUC-Xtr-132742	Xenopus tropicalis	97.81	2.00e-104	370.00
IUUC-Xma-133558	Xiphophorus maculatus	86.49	9.00e-89	318.00
IUUC-Yli-134467	Yarrowia lipolytica	55.49	3.00e-58	217.00
IUUC-Zma-135527	Zea mays	64.84	1.00e-62	232.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence