iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045775

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

Ubiquitin carboxyl-terminal hydrolase 27; Deubiquitinating enzyme 27; Ubiquitin carboxyl-terminal hydrolase 22-like; Ubiquitin thioesterase 27; Ubiquitin-specific-processing protease 27; X-linked ubiquitin carboxyl-terminal hydrolase 27

Genbank Nucleotide ID

AF238380

Gene Name

USP27X; USP22L; USP27

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000273820.1	ENST00000621775.1	ENSP00000483631.1

Annotation

Cancer Mutation
COSMIC	CGAP
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	TCGA	ICGC	COSMIC
HPM
DNA & RNA Element
miRTarBase	microRNA	TRANSFAC	SomamiR
RAID2
Protein-protein Interaction
IID	iRefIndex	HINT	InWeb_IM
Disease-associated Information
ClinVar	OMIM
Post-translational Modifications (PTMs)
CPLM	PhosphositePlus	dbPTM	BioGRID
mUbiSiDa
DNA Methylation
TCGA	ICGC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	19615732

Classification

Family	E-value	Score	Start	End
DUB/USP	2.10e-90	305.0	79	421

Active Site

Position(s)	Description	Evidence
87	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
380	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqqDaq 93
           Gl NlgNtC+mN+++Q+L+++p lr+++l+ ++++++    ++p+ +l++++ +lf+el++++ +   p +ll+ ++  a+++ gy+qqDa+
   Q: 79   GLINLGNTCFMNCIVQALTHTPILRDFFLS-DRHRCEM---PSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAH 166
           9*****************************.9999987...589************************************************ PP
   S: 94   EflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkke 185
           Efl   ld l++++k                  +  +  +++++ +++i+++F g l+s+++c++c+ vs++ +p +d+sl++pg+ ++  +
   Q: 167  EFLIAALDVLHRHCKG----------------DDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTTIDPCWDISLDLPGSCTSFWP 242
           **************22................2344668899999****************************************9987666 PP
   S: 186  ...................slqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefple 258
                              +l+dcL+ ft++e+l ++ k++c +c+++qe++k+l+++klP v ++h+kRf  +++ k+r+ki+++++fple
   Q: 243  mspgressvngeshipgitTLTDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRF--EHSAKQRRKITTYISFPLE 332
           99*********************************************************************..55667999*********** PP
   S: 259  Ldlspfcts............peekqkekkkYeLiavvvHsGslssGHYvayvknekgkWllfdDskVtevkeeevleetgstaYvlfYkrr 338
           Ld++pf++s            p+++ ++++kY+L+avv+H+G+l+sGHY+++++++k++W+++dD  +t+ + ++vl    s++Y+lfY+++
   Q: 333  LDMTPFMASskesrmngqlqlPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLD---SEGYLLFYHKQ 421
           *********5555555555554444444**************************************************...********985 PP

Organism

Homo sapiens

Functional Description
(View)

Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.

Protein Sequence
(Fasta)

MCKDYVYDKD IEQIAKEEQG EALKLQASTS TEVSHQQCSV PGLGEKFPTW ETTKPELELL 60
GHNPRRRRIT SSFTIGLRGL INLGNTCFMN CIVQALTHTP ILRDFFLSDR HRCEMPSPEL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CCCCGGCCCC CGCCCCGGCC CCGGCCCCGG CGCGGCCCTG GGGGCGGATG CCTGGATGTG 60
GATTTTGCCG TGGGGCCACC AGGCTGTTCT CACGTGAACA GCTTTAAGGT GGGAGAGAAC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0225--Disease mutation
KW-0378--Hydrolase
KW-0991--Mental retardation
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:1990380--F:Lys48-specific deubiquitinase activity
GO:0061578--F:Lys63-specific deubiquitinase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0043065--P:positive regulation of apoptotic process
GO:0016579--P:protein deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination
GO:0050821--P:protein stabilization
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:389856

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Bta-012659	Bos taurus	98.17	0.00e+00	907.00
IUUC-Cfa-020956	Canis familiaris	98.63	0.00e+00	904.00
IUUC-Csa-023350	Chlorocebus sabaeus	99.77	0.00e+00	920.00
IUUC-Dno-029905	Dasypus novemcinctus	98.40	0.00e+00	908.00
IUUC-Dor-030832	Dipodomys ordii	97.93	4.00e-113	401.00
IUUC-Itr-048775	Ictidomys tridecemlineatus	99.54	0.00e+00	919.00
IUUC-Mmr-061013	Microcebus murinus	98.40	0.00e+00	910.00
IUUC-Mmu-064287	Mus musculus	96.35	0.00e+00	891.00
IUUC-Nle-069084	Nomascus leucogenys	100.00	0.00e+00	921.00
IUUC-Oga-088771	Otolemur garnettii	99.09	0.00e+00	911.00
IUUC-Oar-089610	Ovis aries	97.94	0.00e+00	900.00
IUUC-Pan-092253	Papio anubis	100.00	0.00e+00	916.00
IUUC-Ppa-095413	Physcomitrella patens	42.86	4.00e-78	285.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence