iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-064287

UUCD1 version

UUC-MuM-01204

Ensembl Protein ID

ENSMUSP00000137509.1

UniProt Accession

Q8CEG8; B1ATV2; Q8BSW2; Q9JIG5; UBP27_MOUSE

Genbank Protein ID

AAF66953.1; BAC25840.1; BAC26935.1; CAM17475.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 27; Deubiquitinating enzyme 27; Ubiquitin thioesterase 27; Ubiquitin-specific-processing protease 27; X-linked ubiquitin carboxyl-terminal hydrolase 27

Genbank Nucleotide ID

AF229643; AK028249; AK030383; AL663104

Gene Name

Usp27x; Usp27

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000046269.16	ENSMUST00000178293.7	ENSMUSP00000137509.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	TRANSFAC	RAID2
Protein-protein Interaction
IID
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	2.60e-90	304.1	79	421

Active Site

Position(s)	Description	Evidence
87	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
380	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqq 90
           Gl NlgNtC+mN+++Q+L+++p lr+++l+ ++++++    ++p+ +l++++ +lf+el++++ +   p +ll+ ++  a+++ gy+qq
   Q: 79   GLINLGNTCFMNCIVQALTHTPILRDFFLS-DRHRCEM---PSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ 163
           9*****************************.9999987...589********************************************* PP
   S: 91   DaqEflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgk 179
           Da+Efl   ld l++++k                  +  +  +++++ +++i+++F g l+s+++c++c+ vs++ +p +d+sl++pg+
   Q: 164  DAHEFLIAALDVLHRHCKG----------------DDVGKVASNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTTIDPCWDISLDLPGS 236
           *****************22................23446788999******************************************9 PP
   S: 180  eetkke...................slqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksreki 249
            ++  +                   +l+dcL+ ft++e+l ++ k++c +c+++qe++k+l++kklP v ++h+kRf  +++ k+r+ki
   Q: 237  CTSFWPmspgresslngeshipgitTLTDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMKKLPVVACFHFKRF--EHSAKQRRKI 323
           98766699*********************************************************************..55667999** PP
   S: 250  nkkvefpleLdlspfcts............peekqkekkkYeLiavvvHsGslssGHYvayvknekgkWllfdDskVtevkeeevleet 326
           +++++fpleLd++pf++s            p+++ ++++kY+L+avv+H+G+l+sGHY++++++++++W+++dD  +t+ + ++vl   
   Q: 324  TTYISFPLELDMTPFMASsketrvngqlqlPTNSANNENKYSLFAVVNHQGTLESGHYTSFIRHHRDQWFKCDDAVITKASIKDVLD-- 410
           *****************96777777777776666666**************************************************.. PP
   S: 327  gstaYvlfYkrr 338
            s++Y+lfY+++
   Q: 411  -SEGYLLFYHKQ 421
           .********985 PP

Organism

Mus musculus

Functional Description
(View)

Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.

Protein Sequence
(Fasta)

MCKDYVYDID IEQIAKEEQG EALKLQASTS TEVSQQQCSV PGLGEKYPTW ETTKPELELL 60
GHNPRRRRIA SSFTIGLRGL INLGNTCFMN CIVQALTHTP ILRDFFLSDR HRCEMPSPEL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GAGAAGGCAT AGAGGAAGGT GGAGGCGGAG AAGGTGGAGG GGAAGGTGGA GGCGGCCGGG 60
AAGGTGGACG CCACCGGGAA GGTGGAGACA GCAGGGAAGG TGGACGCCAC CGGGAAGGTG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:1990380--F:Lys48-specific deubiquitinase activity
GO:0061578--F:Lys63-specific deubiquitinase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0043065--P:positive regulation of apoptotic process
GO:0016579--P:protein deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination
GO:0050821--P:protein stabilization
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

mmu:54651

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Bta-012659	Bos taurus	96.12	0.00e+00	886.00
IUUC-Cfa-020956	Canis familiaris	95.42	0.00e+00	875.00
IUUC-Csa-023350	Chlorocebus sabaeus	96.12	0.00e+00	889.00
IUUC-Dno-029905	Dasypus novemcinctus	95.89	0.00e+00	885.00
IUUC-Dor-030832	Dipodomys ordii	95.34	2.00e-110	392.00
IUUC-Hsa-045775	Homo sapiens	96.35	0.00e+00	891.00
IUUC-Itr-048775	Ictidomys tridecemlineatus	96.35	0.00e+00	891.00
IUUC-Mmr-061013	Microcebus murinus	95.66	0.00e+00	885.00
IUUC-Nle-069084	Nomascus leucogenys	96.35	0.00e+00	891.00
IUUC-Olu-087695	Ostreococcus lucimarinus	32.51	2.00e-50	192.00
IUUC-Oar-089610	Ovis aries	95.88	0.00e+00	879.00
IUUC-Pan-092253	Papio anubis	96.34	0.00e+00	885.00
IUUC-Ppa-095413	Physcomitrella patens	40.69	4.00e-78	285.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence