iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Psi-093506

Ensembl Protein ID

ENSPSIP00000020549.1

UniProt Accession

K7GJT8; K7GJT8_PELSI

Genbank Protein ID

ENSPSIP00000020549

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Genbank Nucleotide ID

AGCU01109403

Gene Name

RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSPSIG00000018185.1	ENSPSIT00000020646.1	ENSPSIP00000020549.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	5.80e-07	30.0	409	447

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C iC e f +  ++l+C H f+  C++ w       +++  CP+Cr
   Q: 409  CTICSEYFIE-AVTLNCAHSFCSYCINEW------MKRKVECPICR 447
           ********99.899***************......**********8 PP

Organism

Pelodiscus sinensis

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

LVGPGRLARI QPTGTRKKVL RRPSSEPTAA LFLKPGEEVT VGRGFGLTYQ LVSKTCPLMI 60
SRYHCAFKQN AQGQWTVTDN KSLNGVWLNK ERLDPFKAYT IGEGDHIQLG VPLDNKETAE 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CTGGTGGGTC CAGGCAGGCT GGCCAGGATC CAGCCCACTG GCACCAGGAA GAAGGTGCTC 60
AGGAGGCCGA TGATCAGGGC TCGACAAACC AGTGAATCTA CTCGCCCATG ACGAGTAGAT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0000151--C:ubiquitin ligase complex
GO:0003682--F:chromatin binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0006302--P:double-strand break repair
GO:0070535--P:histone H2A K63-linked ubiquitination
GO:0034244--P:negative regulation of transcription elongation from RNA polymerase II promoter
GO:0045739--P:positive regulation of DNA repair
GO:0051865--P:protein autoubiquitination
GO:0070936--P:protein K48-linked ubiquitination
GO:0010212--P:response to ionizing radiation
GO:0006511--P:ubiquitin-dependent protein catabolic process

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	61.86	2.00e-124	439.00
IUUC-Apl-003664	Anas platyrhynchos	89.13	4.00e-46	176.00
IUUC-Aca-005036	Anolis carolinensis	68.35	4.00e-148	517.00
IUUC-Ame-011537	Astyanax mexicanus	43.72	2.00e-83	302.00
IUUC-Bgr-012234	Blumeria graminis	46.51	3.00e-09	55.80
IUUC-Bta-013297	Bos taurus	61.60	8.00e-127	446.00
IUUC-Bci-013613	Botrytis cinerea	44.68	3.00e-09	56.20
IUUC-Cja-019201	Callithrix jacchus	64.11	1.00e-133	469.00
IUUC-Cfa-021041	Canis familiaris	63.24	9.00e-140	489.00
IUUC-Csa-024133	Chlorocebus sabaeus	61.07	1.00e-137	482.00
IUUC-Cho-024624	Choloepus hoffmanni	56.16	7.00e-87	313.00
IUUC-Dre-027918	Danio rerio	42.55	1.00e-83	303.00
IUUC-Dno-029774	Dasypus novemcinctus	62.17	6.00e-136	477.00
IUUC-Dor-030190	Dipodomys ordii	55.43	2.00e-98	352.00
IUUC-Ete-032301	Echinops telfairi	57.99	3.00e-97	348.00
IUUC-Eca-033607	Equus caballus	64.16	1.00e-122	432.00
IUUC-Eeu-034888	Erinaceus europaeus	61.24	1.00e-85	309.00
IUUC-Fca-036075	Felis catus	62.19	6.00e-141	493.00
IUUC-Fal-037569	Ficedula albicollis	76.92	2.00e-157	548.00
IUUC-Gmo-038588	Gadus morhua	38.39	8.00e-66	244.00
IUUC-Gga-040777	Gallus gallus	73.57	1.00e-156	545.00
IUUC-Gac-042463	Gasterosteus aculeatus	42.70	3.00e-61	229.00
IUUC-Ggo-045269	Gorilla gorilla	60.99	1.00e-137	483.00
IUUC-Hsa-046249	Homo sapiens	60.57	2.00e-131	462.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	61.07	1.00e-132	466.00
IUUC-Kpa-049392	Komagataella pastoris	40.00	3.00e-09	55.80
IUUC-Lch-049805	Latimeria chalumnae	53.07	2.00e-114	405.00
IUUC-Loc-052377	Lepisosteus oculatus	48.59	2.00e-88	319.00
IUUC-Mcc-055344	Macaca mulatta	60.86	1.00e-137	482.00
IUUC-Meu-055950	Macropus eugenii	62.56	1.00e-113	403.00
IUUC-Mor-056982	Magnaporthe oryzae	45.24	2.00e-08	53.50
IUUC-Mla-059052	Melampsora laricipopulina	34.18	3.00e-11	61.20
IUUC-Mga-060049	Meleagris gallopavo	73.88	6.00e-164	570.00
IUUC-Mmr-061431	Microcebus murinus	62.35	2.00e-130	458.00
IUUC-Mdo-062016	Monodelphis domestica	69.23	3.00e-124	440.00
IUUC-Mmu-063235	Mus musculus	56.85	1.00e-119	422.00
IUUC-Mpu-066449	Mustela putorius furo	63.39	5.00e-146	510.00
IUUC-Mlu-067588	Myotis lucifugus	61.32	7.00e-128	450.00
IUUC-Nle-069192	Nomascus leucogenys	61.19	1.00e-137	483.00
IUUC-Opr-070300	Ochotona princeps	49.08	6.00e-93	334.00
IUUC-Ont-071998	Oreochromis niloticus	45.28	7.00e-66	244.00
IUUC-Oan-073610	Ornithorhynchus anatinus	64.91	5.00e-58	216.00
IUUC-Ocu-074603	Oryctolagus cuniculus	56.35	1.00e-127	449.00
IUUC-Ola-086681	Oryzias latipes	41.28	3.00e-74	271.00
IUUC-Oga-088183	Otolemur garnettii	61.68	2.00e-136	478.00
IUUC-Oar-090255	Ovis aries	63.50	2.00e-122	432.00
IUUC-Ptr-091029	Pan troglodytes	62.47	3.00e-134	471.00
IUUC-Pan-092241	Papio anubis	61.07	9.00e-139	486.00
IUUC-Pma-094579	Petromyzon marinus	57.78	6.00e-27	114.00
IUUC-Pfo-097014	Poecilia formosa	45.01	2.00e-65	243.00
IUUC-Pab-098530	Pongo abelii	63.12	2.00e-136	479.00
IUUC-Pca-100939	Procavia capensis	52.58	2.00e-88	319.00
IUUC-Pva-103148	Pteropus vampyrus	58.11	2.00e-126	445.00
IUUC-Rno-105338	Rattus norvegicus	59.31	4.00e-125	441.00
IUUC-Sha-107187	Sarcophilus harrisii	66.34	7.00e-103	367.00
IUUC-Ssl-108393	Sclerotinia sclerotiorum	42.86	1.00e-09	57.00
IUUC-Sar-113356	Sorex araneus	54.01	1.00e-97	349.00
IUUC-Ssc-116290	Sus scrofa	63.64	6.00e-153	533.00
IUUC-Tru-117910	Takifugu rubripes	43.35	3.00e-63	235.00
IUUC-Tsy-119329	Tarsius syrichta	59.51	4.00e-116	410.00
IUUC-Tni-120521	Tetraodon nigroviridis	42.61	5.00e-68	251.00
IUUC-Tme-126989	Tuber melanosporum	34.48	4.00e-07	49.70
IUUC-Ttr-128997	Tursiops truncatus	57.96	2.00e-120	425.00
IUUC-Vda-130024	Verticillium dahliae	32.20	1.00e-07	51.20
IUUC-Vpa-130154	Vicugna pacos	61.36	3.00e-137	481.00
IUUC-Xtr-132805	Xenopus tropicalis	53.70	4.00e-108	384.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence