iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
Post-translational Modifications (PTMs)
dbPPT	dbPTM
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	1.70e-171	571.9	472	959

Active Site

Position(s)	Description	Evidence
653	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGv.....gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveah 93
           YD Qi ++G++ q+kl+ a+v++vGag+lGcE+lKnL+l+Gv     g++tv+D+d +e+snl+rqFLfr++++g+ K+ vaa a++ +n+  n++a 
   Q: 472  YDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVscgtqGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDAL 569
           ************************************************************************************************** PP
   S: 94   eesiteleeeiv...FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsddppqktiPfctlketpnaaehtie 188
           ++++ +++e+++   F ++++vVv+aldn+ ar++v+++c++ ++pl+esgtlG + +++ +i++lte y +s+dpp+k++P+ct+ ++p +++h+++
   Q: 570  QNRVGPETENVFddsFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 667
           **********99************************************************************************************** PP
   S: 189  wa............vlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttinsikqllksfecdivtkskspfwv 274
           wa            + +n +l++  +  +       ++ ++++ +v + l++e+ ++f+++i++a+  f+ ++ n +kql   f+ d  t+ ++pfw+
   Q: 668  WArsefegllektpAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWS 765
           ****999999999977777777766666666667778888889999999999********************************************** PP
   S: 275  .........................................................sfcsnaealqvpekvekdeevvkasap..lylllrale..r 311
                                                                     f++ ++a  v +  +    + +++++  +  l ++l   r
   Q: 766  apkrfprplqfsstdlshinfvmaasilraetfgiptpewaktraglaeavervivpDFEPKKDATIVTDEKATTLSTASVDDAavIDELNAKLVrcR 863
           *********************************************99999999999966666665554444444444444444457555555554126 PP
   S: 312  fekkkgrkpgelsfekddds..avdlvtaaanlraeslgiepkldddlikeiagniipaiattnavvgGlaaqEviKvvsg..kfeplknfflfdae 404
           ++ +++  ++ ++fekddd   ++d+++ +an+ra ++++++ +d+ + k iag+iipaiat +a+  G++++E++Kv++g  k e ++n+f ++a+
   Q: 864  MSLQPEFRMKAIQFEKDDDTnyHMDMIAGLANMRARNYSVPE-VDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGshKVEDYRNTFANLAL 959
           788999************************************.*************************************99999*******99875 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Protein Sequence
(Fasta)

MEPFVVKENI IASASSPMKK RRIDHTESAD GSAINASNSS SIGLNNSIGG NDTVMSMAEF 60
GNDNSNNQEI DEDLHSRQLA VYGRETMRKL FASNVLISGM QGLGVEIAKN IILAGVKSVT 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TTTTTGTTAT ATACCGCCGC TTCTTTCTAC ACAACCTACA AAGCCAAAAG CTTAGTATAC 60
TCTCTCTTTC TACTTTAACA ACAAATTCGC CGCTTATCGC CTCCGATCTT CTCTCAAAGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR032420--E1_4HB
IPR032418--E1_FCCH
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR018965--Ub-activating_enz_E1_C
IPR019572--UBA_E1_Cys
IPR018075--UBQ-activ_enz_E1
IPR018074--UBQ-activ_enz_E1_CS
IPR033127--UBQ-activ_enz_E1_Cys_AS
IPR000011--UBQ/SUMO-activ_enz_E1-like

PROSITE

PS00536--UBIQUITIN_ACTIVAT_1
PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF16191--E1_4HB
PF16190--E1_FCCH
PF09358--E1_UFD
PF00899--ThiF
PF10585--UBA_e1_thiolCys

PRINTS

PR01849--UBIQUITINACT

SMART

SM00985--UBA_e1_C

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0008641--F:small protein activating enzyme activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0016567--P:protein ubiquitination

KEGG

ath:AT5G06460

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000456	Aegilops tauschii	77.70	0.00e+00	1638.00
IUUC-Aml-001311	Ailuropoda melanoleuca	45.81	0.00e+00	895.00
IUUC-Atr-002684	Amborella trichopoda	74.95	0.00e+00	1636.00
IUUC-Apl-004158	Anas platyrhynchos	39.46	0.00e+00	694.00
IUUC-Aca-004620	Anolis carolinensis	45.95	0.00e+00	890.00
IUUC-Aly-005515	Arabidopsis lyrata	96.39	0.00e+00	2097.00
IUUC-Ago-007873	Ashbya gossypii	45.53	0.00e+00	837.00
IUUC-Afl-008455	Aspergillus flavus	44.82	0.00e+00	788.00
IUUC-Afu-009065	Aspergillus fumigatus	44.85	0.00e+00	839.00
IUUC-Ani-009371	Aspergillus nidulans	45.27	0.00e+00	815.00
IUUC-Ang-009737	Aspergillus niger	44.92	0.00e+00	810.00
IUUC-Aor-010007	Aspergillus oryzae	44.82	0.00e+00	788.00
IUUC-Ate-010466	Aspergillus terreus	45.01	0.00e+00	839.00
IUUC-Ame-010933	Astyanax mexicanus	45.57	0.00e+00	872.00
IUUC-Bgr-012281	Blumeria graminis	44.70	0.00e+00	830.00
IUUC-Bta-012767	Bos taurus	46.52	0.00e+00	909.00
IUUC-Bci-013721	Botrytis cinerea	45.97	0.00e+00	801.00
IUUC-Bdi-014843	Brachypodium distachyon	78.89	0.00e+00	1603.00
IUUC-Bol-015665	Brassica oleracea	89.98	0.00e+00	1976.00
IUUC-Bra-017522	Brassica rapa	93.14	0.00e+00	1898.00
IUUC-Cel-018260	Caenorhabditis elegans	37.70	0.00e+00	655.00
IUUC-Cja-019152	Callithrix jacchus	45.15	0.00e+00	901.00
IUUC-Cfa-020622	Canis familiaris	45.25	0.00e+00	858.00
IUUC-Cpo-022526	Cavia porcellus	46.03	0.00e+00	887.00
IUUC-Cre-022702	Chlamydomonas reinhardtii	60.25	0.00e+00	1182.00
IUUC-Csa-023780	Chlorocebus sabaeus	46.33	0.00e+00	900.00
IUUC-Cho-024682	Choloepus hoffmanni	37.05	6.00e-106	378.00
IUUC-Cin-025790	Ciona intestinalis	44.57	0.00e+00	877.00
IUUC-Csv-026084	Ciona savignyi	42.65	0.00e+00	859.00
IUUC-Cgl-026512	Colletotrichum gloeosporioides	46.24	0.00e+00	875.00
IUUC-Dre-028149	Danio rerio	45.86	0.00e+00	890.00
IUUC-Dno-028884	Dasypus novemcinctus	46.13	0.00e+00	899.00
IUUC-Dor-030229	Dipodomys ordii	44.37	0.00e+00	856.00
IUUC-Dse-031524	Dothistroma septosporum	43.44	0.00e+00	822.00
IUUC-Ete-033124	Echinops telfairi	38.51	2.00e-128	452.00
IUUC-Eca-033330	Equus caballus	45.95	0.00e+00	897.00
IUUC-Eeu-035323	Erinaceus europaeus	40.85	6.00e-153	534.00
IUUC-Fca-036255	Felis catus	46.33	0.00e+00	892.00
IUUC-Fal-036848	Ficedula albicollis	39.07	0.00e+00	701.00
IUUC-Fox-038007	Fusarium oxysporum	45.65	2.00e-132	465.00
IUUC-Fso-038339	Fusarium solani	46.76	0.00e+00	834.00
IUUC-Gmo-039338	Gadus morhua	43.64	0.00e+00	843.00
IUUC-Ggr-039823	Gaeumannomyces graminis	45.02	0.00e+00	866.00
IUUC-Gga-040747	Gallus gallus	39.71	0.00e+00	698.00
IUUC-Gac-041773	Gasterosteus aculeatus	44.23	0.00e+00	861.00
IUUC-Gma-042755	Glycine max	83.42	0.00e+00	1786.00
IUUC-Ggo-045336	Gorilla gorilla	46.13	0.00e+00	884.00
IUUC-Hsa-045982	Homo sapiens	46.13	0.00e+00	884.00
IUUC-Hvu-047418	Hordeum vulgare	77.90	0.00e+00	1614.00
IUUC-Itr-048460	Ictidomys tridecemlineatus	46.43	0.00e+00	902.00
IUUC-Kpa-049112	Komagataella pastoris	46.24	0.00e+00	875.00
IUUC-Lch-050371	Latimeria chalumnae	44.83	0.00e+00	895.00
IUUC-Lpe-051299	Leersia perrieri	75.60	0.00e+00	1606.00
IUUC-Loc-052936	Lepisosteus oculatus	45.76	0.00e+00	901.00
IUUC-Lma-053315	Leptosphaeria maculans	46.36	0.00e+00	889.00
IUUC-Laf-053948	Loxodonta africana	45.23	0.00e+00	876.00
IUUC-Mcc-055843	Macaca mulatta	46.33	0.00e+00	901.00
IUUC-Meu-056258	Macropus eugenii	45.62	0.00e+00	881.00
IUUC-Mor-057269	Magnaporthe oryzae	44.10	0.00e+00	841.00
IUUC-Mpo-057435	Magnaporthe poae	45.11	0.00e+00	840.00
IUUC-Mla-059176	Melampsora laricipopulina	47.32	0.00e+00	872.00
IUUC-Mga-059721	Meleagris gallopavo	40.06	0.00e+00	675.00
IUUC-Mmr-061405	Microcebus murinus	45.45	0.00e+00	862.00
IUUC-Mdo-062032	Monodelphis domestica	45.42	0.00e+00	871.00
IUUC-Mmu-063721	Mus musculus	46.05	0.00e+00	902.00
IUUC-Mac-065505	Musa acuminata	77.10	0.00e+00	1642.00
IUUC-Mpu-066409	Mustela putorius furo	46.23	0.00e+00	904.00
IUUC-Mlu-067217	Myotis lucifugus	45.84	0.00e+00	891.00
IUUC-Nfi-068407	Neosartorya fischeri	45.09	0.00e+00	820.00
IUUC-Ncr-068698	Neurospora crassa	45.88	0.00e+00	865.00
IUUC-Nle-070072	Nomascus leucogenys	43.50	0.00e+00	809.00
IUUC-Opr-070715	Ochotona princeps	35.14	4.00e-151	528.00
IUUC-Ont-071671	Oreochromis niloticus	45.60	0.00e+00	887.00
IUUC-Oan-073268	Ornithorhynchus anatinus	40.89	0.00e+00	643.00
IUUC-Ocu-074544	Oryctolagus cuniculus	46.62	0.00e+00	907.00
IUUC-Oba-075120	Oryza barthii	78.57	0.00e+00	1615.00
IUUC-Obr-076557	Oryza brachyantha	79.17	0.00e+00	1627.00
IUUC-Ogl-077887	Oryza glaberrima	78.57	0.00e+00	1618.00
IUUC-Ogu-078305	Oryza glumaepatula	75.30	0.00e+00	1597.00
IUUC-Oin-079370	Oryza indica	76.34	0.00e+00	1601.00
IUUC-Olo-081089	Oryza longistaminata	75.50	0.00e+00	1600.00
IUUC-Ome-081942	Oryza meridionalis	78.97	0.00e+00	1624.00
IUUC-Oni-082365	Oryza nivara	78.97	0.00e+00	1625.00
IUUC-Opu-083977	Oryza punctata	78.57	0.00e+00	1617.00
IUUC-Oru-084469	Oryza rufipogon	75.50	0.00e+00	1600.00
IUUC-Osa-085949	Oryza sativa	78.57	0.00e+00	1644.00
IUUC-Ola-087002	Oryzias latipes	45.69	0.00e+00	888.00
IUUC-Oga-088594	Otolemur garnettii	46.13	0.00e+00	884.00
IUUC-Oar-090068	Ovis aries	46.33	0.00e+00	904.00
IUUC-Ptr-090609	Pan troglodytes	39.01	0.00e+00	697.00
IUUC-Pan-092114	Papio anubis	46.33	0.00e+00	902.00
IUUC-Psi-093489	Pelodiscus sinensis	38.58	0.00e+00	696.00
IUUC-Pno-095154	Phaeosphaeria nodorum	44.70	0.00e+00	851.00
IUUC-Ppa-095227	Physcomitrella patens	71.53	0.00e+00	1498.00
IUUC-Pfo-097184	Poecilia formosa	44.88	0.00e+00	878.00
IUUC-Pab-097861	Pongo abelii	46.03	0.00e+00	869.00
IUUC-Pca-100347	Procavia capensis	42.94	0.00e+00	713.00
IUUC-Pva-102584	Pteropus vampyrus	46.52	0.00e+00	928.00
IUUC-Pgr-103336	Puccinia graminis	45.58	0.00e+00	823.00
IUUC-Ptt-103939	Puccinia triticina	46.56	0.00e+00	824.00
IUUC-Pte-104200	Pyrenophora teres	45.99	0.00e+00	887.00
IUUC-Pyt-104441	Pyrenophora triticirepentis	45.83	0.00e+00	882.00
IUUC-Rno-105462	Rattus norvegicus	46.13	0.00e+00	884.00
IUUC-Sce-106245	Saccharomyces cerevisiae	45.03	0.00e+00	877.00
IUUC-Sha-107142	Sarcophilus harrisii	45.80	0.00e+00	893.00
IUUC-Spo-108034	Schizosaccharomyces pombe	44.37	0.00e+00	831.00
IUUC-Ssl-108497	Sclerotinia sclerotiorum	45.58	0.00e+00	826.00
IUUC-Sit-110281	Setaria italica	78.87	0.00e+00	1640.00
IUUC-Sar-113737	Sorex araneus	46.28	0.00e+00	894.00
IUUC-Sbi-114520	Sorghum bicolor	79.27	0.00e+00	1649.00
IUUC-Sre-115148	Sporisorium reilianum	45.01	0.00e+00	820.00
IUUC-Ssc-115777	Sus scrofa	45.90	0.00e+00	899.00
IUUC-Tgu-116565	Taeniopygia guttata	38.74	0.00e+00	691.00
IUUC-Tru-118742	Takifugu rubripes	45.99	0.00e+00	850.00
IUUC-Tsy-119436	Tarsius syrichta	34.12	3.00e-164	572.00
IUUC-Tni-120401	Tetraodon nigroviridis	45.50	0.00e+00	848.00
IUUC-Tre-122009	Trichoderma reesei	46.91	0.00e+00	836.00
IUUC-Tvi-122259	Trichoderma virens	47.15	0.00e+00	838.00
IUUC-Tae-125113	Triticum aestivum	78.20	0.00e+00	1587.00
IUUC-Tur-126330	Triticum urartu	77.80	0.00e+00	1644.00
IUUC-Tme-127055	Tuber melanosporum	43.55	0.00e+00	790.00
IUUC-Tbe-127714	Tupaia belangeri	36.65	4.00e-141	494.00
IUUC-Ttr-128331	Tursiops truncatus	46.03	0.00e+00	887.00
IUUC-Uma-129387	Ustilago maydis	45.44	0.00e+00	830.00
IUUC-Vda-129977	Verticillium dahliae	47.22	0.00e+00	807.00
IUUC-Vpa-130716	Vicugna pacos	38.24	0.00e+00	681.00
IUUC-Xtr-132026	Xenopus tropicalis	40.59	0.00e+00	681.00
IUUC-Xma-133805	Xiphophorus maculatus	44.91	0.00e+00	860.00
IUUC-Zma-135052	Zea mays	78.64	0.00e+00	1664.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence