iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Tae-125679

Ensembl Protein ID

TRIAE_CS42_5BL_TGACv1_405648_AA1332140.2

UniProt Accession

W5FIL9; W5FIL9_WHEAT

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
TRIAE_CS42_5BL_TGACv1_405648_AA1332140	TRIAE_CS42_5BL_TGACv1_405648_AA1332140.1	TRIAE_CS42_5BL_TGACv1_405648_AA1332140.1
TRIAE_CS42_5BL_TGACv1_405648_AA1332140	TRIAE_CS42_5BL_TGACv1_405648_AA1332140.2	TRIAE_CS42_5BL_TGACv1_405648_AA1332140.2

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	3.80e-105	355.1	57	563

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedv 69
           YDRQ+r+wG+++q++l++a+++l+++g++G+E lKnLvl+G+gs+tvvD+++ve+s+l+++FL+++   
   Q: 57   YDRQLRIWGDQGQAALEKASICLLNCGPTGTEALKNLVLGGIGSVTVVDGSKVEQSDLGNNFLLDEGCL 125
           ********************************************************************* PP
   S: 70   gksKAevaaealkelnpdvnveaheesiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpl 137
           g+s+A++ + +l+eln+ v+++ +ees+ +  ++++ Ff++f+vV++++ ++++ +k++++c++a++ l
   Q: 126  GQSRAKSICSFLQELNDAVKAKYVEESPATMIDTNPsFFSQFTVVLATQLPESSLLKLDSICRSANIVL 194
           **********************************99********************************* PP
   S: 138  iesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn.........aaehtiewavlfn 194
           + ++++Gl G vrv+ik++ +++s++d   d++++ +P+++lk++           +++++++++v+++
   Q: 195  VAARSYGLTGVVRVSIKEHCVIESKPDhslDDLRLHNPWPELKQFAKsidicdkdpVVHKHTPYIVILA 263
           *********************************************99999999999************* PP
   S: 195  klleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfec 262
            l+e++a +++++ +++++ek+e+kd++++ + + de+n++ea+e + k   +  i++ i+q++++ + 
   Q: 264  RLAEKWADAHDGQLPSTRQEKREFKDLIRAHMLNVDEDNYKEAVESSYKVSVTPGISDeIRQIIDD-SS 331
           ******************************************************************.55 PP
   S: 263  divtkskspfwv......................................................... 274
            +v+ s+s+fwv                                                         
   Q: 332  SEVNFSSSDFWVlvaslkefianegngelplegtipdmtslteyyvslqkiyqakaesdclaiehrvks 400
           599****************************************************************** PP
   S: 275  ..................sfcsnaealq..............vpekvekdeevvkasap.....lylll 306
                             +fc+n+++l+              v     + +++++++++     +y+ll
   Q: 401  ilkrigrdpdsisracikTFCKNTRKLKvcryrsmeeefsspV---LSEVKKYFADEDScfamnFYVLL 466
           ****************************666665555543221...223333333333367788***** PP
   S: 307  ralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattna 374
           ra++r++++++r pg ++ e  +d +++l++aa +   +++g+++  l++dli+e+++++ ++i++++a
   Q: 467  RAVDRLAANYSRLPGIFDSEIGED-VPRLKEAAVSVL-SDMGLKGsSLSEDLIAEVCRFAGAEIHPVAA 533
           **************9999999999.999999888877.******************************* PP
   S: 375  vvgGlaaqEviKvvsgkfeplknfflfdae 404
           ++gG+a+qEviK+v+++f+pl+ +f+f+++
   Q: 534  FIGGVASQEVIKLVTKQFVPLNGTFIFNGI 563
           ***************************986 PP

Organism

Triticum aestivum

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MHARAGGKRL HHHLRIARAA SREGSRFPTR FHFRSSAAAT ASAAMAAAAV AEPKTKYDRQ 60
LRIWGDQGQA ALEKASICLL NCGPTGTEAL KNLVLGGIGS VTVVDGSKVE QSDLGNNFLL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CAGACGGCCA GGTTTGCTCT GAGACAGAGG AGGCTGAGAG ATACGCGTGA TCCGGCCGTT 60
CCTTTGGAAC TGGATGCACG CACGAGCTGG TGGAAAACGC CTGCACCACC ACCTCCGCAT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Apl-003446	Anas platyrhynchos	43.30	1.00e-120	427.00
IUUC-Aca-004450	Anolis carolinensis	41.83	4.00e-125	442.00
IUUC-Bta-012583	Bos taurus	43.51	3.00e-130	459.00
IUUC-Cel-018456	Caenorhabditis elegans	30.64	2.00e-70	260.00
IUUC-Cja-020007	Callithrix jacchus	43.48	1.00e-128	453.00
IUUC-Cfa-020324	Canis familiaris	43.32	1.00e-130	460.00
IUUC-Cpo-022248	Cavia porcellus	39.60	8.00e-70	258.00
IUUC-Csa-023149	Chlorocebus sabaeus	40.30	2.00e-101	363.00
IUUC-Cin-025763	Ciona intestinalis	40.94	2.00e-111	396.00
IUUC-Csv-026113	Ciona savignyi	40.15	4.00e-107	382.00
IUUC-Dno-030033	Dasypus novemcinctus	43.14	4.00e-112	399.00
IUUC-Dor-030513	Dipodomys ordii	37.60	2.00e-39	157.00
IUUC-Eca-033992	Equus caballus	40.77	5.00e-100	358.00
IUUC-Fal-037600	Ficedula albicollis	42.48	1.00e-117	417.00
IUUC-Gmo-038562	Gadus morhua	40.91	1.00e-119	424.00
IUUC-Gga-041129	Gallus gallus	43.16	1.00e-121	430.00
IUUC-Ggo-045611	Gorilla gorilla	43.07	8.00e-128	451.00
IUUC-Hsa-046563	Homo sapiens	43.32	5.00e-129	455.00
IUUC-Itr-048066	Ictidomys tridecemlineatus	43.51	9.00e-129	454.00
IUUC-Loc-051917	Lepisosteus oculatus	38.79	2.00e-107	383.00
IUUC-Mcc-055117	Macaca mulatta	42.99	3.00e-126	446.00
IUUC-Meu-056761	Macropus eugenii	36.55	3.00e-94	339.00
IUUC-Mga-060144	Meleagris gallopavo	42.64	6.00e-118	418.00
IUUC-Mmr-061325	Microcebus murinus	42.50	1.00e-125	444.00
IUUC-Mdo-062532	Monodelphis domestica	43.92	2.00e-129	456.00
IUUC-Mpu-066716	Mustela putorius furo	43.29	3.00e-120	426.00
IUUC-Mlu-067459	Myotis lucifugus	43.81	4.00e-131	462.00
IUUC-Nle-070034	Nomascus leucogenys	42.08	2.00e-122	432.00
IUUC-Opr-070552	Ochotona princeps	39.19	2.00e-97	350.00
IUUC-Oan-073389	Ornithorhynchus anatinus	39.69	1.00e-88	321.00
IUUC-Ocu-074911	Oryctolagus cuniculus	43.89	2.00e-130	459.00
IUUC-Ola-086420	Oryzias latipes	38.83	3.00e-77	282.00
IUUC-Oar-089196	Ovis aries	43.26	2.00e-128	453.00
IUUC-Ptr-090617	Pan troglodytes	43.32	3.00e-129	456.00
IUUC-Pan-092761	Papio anubis	43.51	3.00e-130	459.00
IUUC-Psi-093191	Pelodiscus sinensis	40.58	6.00e-122	431.00
IUUC-Pma-094380	Petromyzon marinus	41.13	1.00e-110	394.00
IUUC-Pab-098255	Pongo abelii	44.80	2.00e-108	386.00
IUUC-Pca-100807	Procavia capensis	53.76	1.00e-55	211.00
IUUC-Ptt-103889	Puccinia triticina	28.85	5.00e-63	236.00
IUUC-Sha-106770	Sarcophilus harrisii	50.38	4.00e-74	271.00
IUUC-Spo-108276	Schizosaccharomyces pombe	35.26	3.00e-83	303.00
IUUC-Sar-113083	Sorex araneus	34.43	2.00e-86	313.00
IUUC-Tgu-116772	Taeniopygia guttata	43.07	9.00e-118	417.00
IUUC-Tru-118686	Takifugu rubripes	40.11	6.00e-123	435.00
IUUC-Tsy-119111	Tarsius syrichta	46.01	3.00e-64	240.00
IUUC-Ttr-128465	Tursiops truncatus	40.23	4.00e-114	405.00
IUUC-Vpa-130529	Vicugna pacos	40.83	1.00e-118	421.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence