iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045784

UUCD1 version

UUC-HoS-00224

Ensembl Protein ID

ENSP00000262971.1

UniProt Accession

Q8N2W9; O75926; Q96G19; Q9UN16; PIAS4_HUMAN

Genbank Protein ID

AAC36703.1; AAH10047.2; AAH29874.1; AAH66895.1; AAD45155.1

Protein Name

E3 SUMO-protein ligase PIAS4; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; RING-type E3 ubiquitin transferase PIAS4

Genbank Nucleotide ID

AF077952; BC010047; BC029874; BC066895; AF164437

Gene Name

PIAS4; PIASG

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000105229.6	ENST00000262971.2	ENSP00000262971.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	TargetScan	RepTar	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	PHOSIDA	BioGRID
mUbiSiDa
DNA Methylation
TCGA	ICGC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/RING/RING	RING	20429939; 19625650; 19450230; 18063693; 17456046; 15713642; 20228053; 16622421; 19615980; 18974227; 17298944; 21079245; 20661221; 20471636; 17696781; 17585876

Classification

Family	E-value	Score	Start	End
E3 activity/RING/RING	0.001	21.2	336	369

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 11   gplvlpCgHvfhadClqkw.pglknssskefrCPlCr 46
           + +   C H   ++C+ ++ ++++n+++++++CP+C+
   Q: 336  PCRAETCAH---LQCFDAVfYLQMNEKKPTWMCPVCD 369
           222333555...555555557899************5 PP

Organism

Homo sapiens

Functional Description
(View)

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

Protein Sequence
(Fasta)

MAAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKE 60
LYETRYAKKN SEPAPQPHRP LDPLTMHSTY DRAGAVPRTP LAGPNIDYPV LYGKYLNGLG 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGAGGAGCGC GCGCGGGTGC TAATTGGCCC GGGCGGCGGC CCCGCCCGCG AGTGGTGAGC 60
GGTCACGTGA CGCGACGGCT GGGGGCTCCC GGCGCGGGGG ACGCTGGTGA CCAAGATGGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0879--Wnt signaling pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR027224--PIAS4
IPR023321--PINIT
IPR003034--SAP_dom
IPR004181--Znf_MIZ

PROSITE

PS51466--PINIT
PS50800--SAP
PS51044--ZF_SP_RING

Pfam

PF14324--PINIT
PF02891--zf-MIZ

SMART

SM00513--SAP

Gene Ontology

GO:0005737--C:cytoplasm
GO:0016363--C:nuclear matrix
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016605--C:PML body
GO:1990234--C:transferase complex
GO:0003677--F:DNA binding
GO:0061665--F:SUMO ligase activity
GO:0019789--F:SUMO transferase activity
GO:0003714--F:transcription corepressor activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0008270--F:zinc ion binding
GO:0006303--P:double-strand break repair via nonhomologous end joining
GO:0032088--P:negative regulation of NF-kappaB transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0010804--P:negative regulation of tumor necrosis factor-mediated signaling pathway
GO:1902231--P:positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
GO:1902174--P:positive regulation of keratinocyte apoptotic process
GO:0033235--P:positive regulation of protein sumoylation
GO:0016925--P:protein sumoylation
GO:0006351--P:transcription, DNA-templated
GO:0016055--P:Wnt signaling pathway

KEGG

hsa:51588

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001745	Ailuropoda melanoleuca	92.53	0.00e+00	889.00
IUUC-Apl-003765	Anas platyrhynchos	81.98	0.00e+00	814.00
IUUC-Ame-010763	Astyanax mexicanus	59.78	3.00e-146	511.00
IUUC-Bta-012369	Bos taurus	94.54	0.00e+00	909.00
IUUC-Cja-019450	Callithrix jacchus	97.25	0.00e+00	929.00
IUUC-Cfa-020933	Canis familiaris	93.59	0.00e+00	900.00
IUUC-Cpo-022112	Cavia porcellus	92.41	0.00e+00	868.00
IUUC-Csa-023913	Chlorocebus sabaeus	86.77	0.00e+00	766.00
IUUC-Cgl-026533	Colletotrichum gloeosporioides	27.72	3.00e-34	139.00
IUUC-Dre-027609	Danio rerio	59.01	6.00e-142	497.00
IUUC-Dno-029664	Dasypus novemcinctus	74.88	5.00e-154	538.00
IUUC-Ete-032988	Echinops telfairi	93.06	1.00e-173	602.00
IUUC-Eca-034430	Equus caballus	91.76	0.00e+00	872.00
IUUC-Fca-035968	Felis catus	97.60	3.00e-169	587.00
IUUC-Fal-037449	Ficedula albicollis	83.56	0.00e+00	800.00
IUUC-Fso-038103	Fusarium solani	34.72	1.00e-26	114.00
IUUC-Gmo-038925	Gadus morhua	60.78	3.00e-139	488.00
IUUC-Ggr-039896	Gaeumannomyces graminis	32.48	1.00e-29	124.00
IUUC-Gga-040362	Gallus gallus	85.91	0.00e+00	848.00
IUUC-Gac-042353	Gasterosteus aculeatus	59.05	4.00e-133	468.00
IUUC-Ggo-044810	Gorilla gorilla	97.06	0.00e+00	900.00
IUUC-Itr-048486	Ictidomys tridecemlineatus	90.89	0.00e+00	885.00
IUUC-Lch-049727	Latimeria chalumnae	72.22	9.00e-153	533.00
IUUC-Loc-052354	Lepisosteus oculatus	53.71	3.00e-122	431.00
IUUC-Laf-054261	Loxodonta africana	92.82	0.00e+00	906.00
IUUC-Mcc-054931	Macaca mulatta	98.82	0.00e+00	933.00
IUUC-Meu-056468	Macropus eugenii	87.48	0.00e+00	867.00
IUUC-Mpo-057405	Magnaporthe poae	33.06	1.00e-28	121.00
IUUC-Mga-059981	Meleagris gallopavo	89.09	0.00e+00	709.00
IUUC-Mvi-060421	Microbotryum violaceum	29.22	2.00e-27	117.00
IUUC-Mmr-061181	Microcebus murinus	92.09	0.00e+00	870.00
IUUC-Mdo-062345	Monodelphis domestica	87.28	0.00e+00	869.00
IUUC-Mmu-063197	Mus musculus	86.80	0.00e+00	853.00
IUUC-Mpu-066275	Mustela putorius furo	92.22	0.00e+00	899.00
IUUC-Mlu-067194	Myotis lucifugus	93.79	0.00e+00	903.00
IUUC-Ncr-068929	Neurospora crassa	27.70	3.00e-27	116.00
IUUC-Ont-072227	Oreochromis niloticus	59.05	7.00e-139	487.00
IUUC-Obr-076179	Oryza brachyantha	42.86	2.00e-20	94.70
IUUC-Ola-086865	Oryzias latipes	58.78	5.00e-138	484.00
IUUC-Oga-088945	Otolemur garnettii	95.89	0.00e+00	934.00
IUUC-Oar-089412	Ovis aries	91.99	0.00e+00	869.00
IUUC-Ptr-091550	Pan troglodytes	87.11	0.00e+00	652.00
IUUC-Pan-092183	Papio anubis	99.02	0.00e+00	948.00
IUUC-Psi-093003	Pelodiscus sinensis	83.95	0.00e+00	830.00
IUUC-Pfo-096704	Poecilia formosa	59.10	5.00e-141	494.00
IUUC-Pab-097923	Pongo abelii	99.74	0.00e+00	783.00
IUUC-Pca-100249	Procavia capensis	76.58	2.00e-143	501.00
IUUC-Pva-102562	Pteropus vampyrus	89.48	0.00e+00	836.00
IUUC-Rno-105762	Rattus norvegicus	87.38	0.00e+00	857.00
IUUC-Sce-106398	Saccharomyces cerevisiae	34.10	1.00e-28	121.00
IUUC-Sha-107253	Sarcophilus harrisii	86.50	0.00e+00	860.00
IUUC-Sja-107662	Schizosaccharomyces japonicus	31.41	7.00e-29	122.00
IUUC-Sar-113149	Sorex araneus	85.82	1.00e-127	450.00
IUUC-Ssc-115241	Sus scrofa	94.15	0.00e+00	914.00
IUUC-Tgu-116884	Taeniopygia guttata	84.33	0.00e+00	803.00
IUUC-Tru-118031	Takifugu rubripes	59.50	7.00e-145	507.00
IUUC-Tni-119766	Tetraodon nigroviridis	59.46	5.00e-140	491.00
IUUC-Ttr-128556	Tursiops truncatus	84.14	0.00e+00	738.00
IUUC-Xtr-131866	Xenopus tropicalis	77.22	0.00e+00	763.00
IUUC-Xma-133963	Xiphophorus maculatus	59.37	5.00e-135	474.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence