iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046726

UUCD1 version

UUC-HoS-00379

Ensembl Protein ID

ENSP00000262435.9

UniProt Accession

Q9HAU4; Q52LL1; Q9H260; SMUF2_HUMAN

Genbank Protein ID

AAG45422.1; AAG25641.1; AAG50421.1; AAH93876.1; AAI11946.1

Protein Name

E3 ubiquitin-protein ligase SMURF2; HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2

Genbank Nucleotide ID

AF310676; AF301463; AY014180; BC093876; BC111945

Gene Name

SMURF2

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000108854.15	ENST00000582081.5	ENSP00000463531.1
ENSG00000108854.15	ENST00000578386.5	ENSP00000464432.1
ENSG00000108854.15	ENST00000262435.13	ENSP00000262435.9
ENSG00000108854.15	ENST00000585301.1	ENSP00000463804.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	TargetScan	RepTar	miRNAMap
SomamiR	miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	BioGRID
DNA Methylation
MethyCancer	TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	16950122; 18927080; 19583833; 19255252; 20819168; 14562029; 20073064; 11389444; 11163210; 18671942; 14755250; 20858899

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	5.20e-129	432.1	415	747

Active Site

Position(s)	Description	Evidence
716	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlpls 93
           ++dl krl+++f+gEegld+gGv+re+l+lls+e+ np++glf+ys +d +tlq+npds + npehl++F+f+Gr++g+a+++g+++d  ++
   Q: 415  PKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAV-NPEHLSYFHFVGRIMGMAVFHGHYIDGGFT 505
           678999*****************************************************98.89**************************** PP
   S: 94   ksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvveyrlkeg 185
            +fyk+llgk+++l+d+e vdp+l++sl +i+en   di+   ld tf+v+++ +ge+ ++eLkp+G++ipV++eNk+eYv+l+v++r+ +g
   Q: 506  LPFYKQLLGKSITLDDMELVDPDLHNSLVWILEN---DITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRG 593
           ********************************99...9***8.************************************************* PP
   S: 186  ikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlg 277
           i+ q+ a+++Gfnevip++ll+ F+e+elel+icG  +idv+d+k +t+ k+ +t+ds+ ++wFw+++e f++e r+++LqFvTgssr+Pl+
   Q: 594  IEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKH-CTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQ 684
           **************************************************99.*************************************** PP
   S: 278  Gfaelk....prlfvirk.dasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Gf++l+    prlf+i++ da +++lP+++TCfn++++P+Y+s+e+l+ekLl+Aieet gF +
   Q: 685  GFKALQgaagPRLFTIHQiDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747
           *************************************************************76 PP

Organism

Homo sapiens

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Protein Sequence
(Fasta)

MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK 60
WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGGGAGCTCG GCGTCCCGCT CCCTCCGCGG CTCATGGCGA CGACTCTCGG CACATCCGGG 60
ACCCTCCGGC CGTGGCGGCC GAGGCGCCGG CTGCTCGGGC CCCAGCCCCG GCCGCTGTGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005829--C:cytosol
GO:0045121--C:membrane raft
GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0000151--C:ubiquitin ligase complex
GO:0042802--F:identical protein binding
GO:0046332--F:SMAD binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0030509--P:BMP signaling pathway
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0030512--P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0090263--P:positive regulation of canonical Wnt signaling pathway
GO:1901165--P:positive regulation of trophoblast cell migration
GO:0016579--P:protein deubiquitination
GO:0000209--P:protein polyubiquitination
GO:0017015--P:regulation of transforming growth factor beta receptor signaling pathway
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0030579--P:ubiquitin-dependent SMAD protein catabolic process
GO:0060071--P:Wnt signaling pathway, planar cell polarity pathway

KEGG

hsa:64750

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001305	Ailuropoda melanoleuca	99.05	0.00e+00	1498.00
IUUC-Apl-003513	Anas platyrhynchos	97.68	0.00e+00	1478.00
IUUC-Aca-005358	Anolis carolinensis	97.21	0.00e+00	1502.00
IUUC-Ame-011320	Astyanax mexicanus	87.86	0.00e+00	1389.00
IUUC-Bta-013399	Bos taurus	99.45	0.00e+00	1491.00
IUUC-Cja-019355	Callithrix jacchus	98.92	0.00e+00	1504.00
IUUC-Cpo-022200	Cavia porcellus	99.45	0.00e+00	1499.00
IUUC-Cin-025126	Ciona intestinalis	65.79	0.00e+00	971.00
IUUC-Csv-026339	Ciona savignyi	64.91	0.00e+00	1005.00
IUUC-Dre-027650	Danio rerio	90.46	0.00e+00	1433.00
IUUC-Dno-029534	Dasypus novemcinctus	99.47	0.00e+00	1528.00
IUUC-Ete-032715	Echinops telfairi	94.66	0.00e+00	1408.00
IUUC-Eca-033580	Equus caballus	99.73	0.00e+00	1503.00
IUUC-Eeu-034945	Erinaceus europaeus	97.56	0.00e+00	1390.00
IUUC-Fal-037275	Ficedula albicollis	96.90	0.00e+00	1469.00
IUUC-Gga-041060	Gallus gallus	94.29	0.00e+00	1431.00
IUUC-Gac-041295	Gasterosteus aculeatus	91.67	0.00e+00	1399.00
IUUC-Lch-050026	Latimeria chalumnae	95.49	0.00e+00	1467.00
IUUC-Loc-051760	Lepisosteus oculatus	88.52	0.00e+00	1375.00
IUUC-Laf-053753	Loxodonta africana	99.59	0.00e+00	1501.00
IUUC-Mcc-054815	Macaca mulatta	99.73	0.00e+00	1533.00
IUUC-Meu-056436	Macropus eugenii	76.13	0.00e+00	1102.00
IUUC-Mmr-060806	Microcebus murinus	99.45	0.00e+00	1499.00
IUUC-Mdo-062805	Monodelphis domestica	97.46	0.00e+00	1493.00
IUUC-Mmu-064034	Mus musculus	99.20	0.00e+00	1523.00
IUUC-Mpu-066762	Mustela putorius furo	99.05	0.00e+00	1497.00
IUUC-Mlu-066981	Myotis lucifugus	99.59	0.00e+00	1500.00
IUUC-Nle-069804	Nomascus leucogenys	100.00	0.00e+00	1531.00
IUUC-Opr-070591	Ochotona princeps	93.97	0.00e+00	1397.00
IUUC-Ont-071239	Oreochromis niloticus	91.99	0.00e+00	1407.00
IUUC-Oan-072699	Ornithorhynchus anatinus	95.71	0.00e+00	1498.00
IUUC-Ocu-074913	Oryctolagus cuniculus	99.73	0.00e+00	1534.00
IUUC-Ola-086597	Oryzias latipes	85.62	0.00e+00	1285.00
IUUC-Oga-089116	Otolemur garnettii	95.90	0.00e+00	1432.00
IUUC-Oar-089366	Ovis aries	99.18	0.00e+00	1493.00
IUUC-Ptr-091382	Pan troglodytes	100.00	0.00e+00	1537.00
IUUC-Psi-093298	Pelodiscus sinensis	97.96	0.00e+00	1457.00
IUUC-Pma-094354	Petromyzon marinus	73.51	0.00e+00	1125.00
IUUC-Pfo-096071	Poecilia formosa	90.40	0.00e+00	1421.00
IUUC-Pab-098682	Pongo abelii	100.00	0.00e+00	1537.00
IUUC-Pca-100516	Procavia capensis	89.83	0.00e+00	637.00
IUUC-Pva-102754	Pteropus vampyrus	98.49	0.00e+00	1462.00
IUUC-Rno-105600	Rattus norvegicus	99.06	0.00e+00	1292.00
IUUC-Sha-107144	Sarcophilus harrisii	97.36	0.00e+00	1355.00
IUUC-Spo-108171	Schizosaccharomyces pombe	40.37	1.00e-163	570.00
IUUC-Sar-113122	Sorex araneus	88.36	0.00e+00	1281.00
IUUC-Tgu-117112	Taeniopygia guttata	97.08	0.00e+00	1497.00
IUUC-Tru-118497	Takifugu rubripes	88.99	0.00e+00	1366.00
IUUC-Tsy-118819	Tarsius syrichta	99.03	0.00e+00	1038.00
IUUC-Tni-120781	Tetraodon nigroviridis	89.77	0.00e+00	1179.00
IUUC-Tme-126897	Tuber melanosporum	39.30	4.00e-153	535.00
IUUC-Tbe-127371	Tupaia belangeri	89.28	0.00e+00	1220.00
IUUC-Ttr-129305	Tursiops truncatus	91.68	0.00e+00	1288.00
IUUC-Vpa-130591	Vicugna pacos	89.32	0.00e+00	1302.00
IUUC-Xtr-132979	Xenopus tropicalis	77.06	0.00e+00	1105.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence