iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000136014.11	ENST00000258499.7	ENSP00000258499.3
ENSG00000136014.11	ENST00000552440.5	ENSP00000448670.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
TargetScan	RepTar	miRNAMap	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
InWeb_IM
Disease-associated Information
ClinVar	GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	BioGRID
DNA Methylation
MethyCancer	TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	20402667; 20195072; 17443180; 14715245

Classification

Family	E-value	Score	Start	End
DUB/USP	2.10e-97	328.0	273	677

Active Site

Position(s)	Description	Evidence
282	Nucleophile
636	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle............kkkeklneenp...................................... 42
           tGl+NlgNtCYmNSvLQ+Ls++  +r+++l+            +k++      +                                      
   Q: 273  TGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKldlnqwlamtasEKTR------Sckhppvtdtvvyqmnecqekdtgfvcsrqsslssglsg 358
           7******************************8887766665543334......044455799999999999999999999999999999999 PP
   S: 43   ................kepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqqDaqEflaflldqleeelkkeknkpkeee 118
                           +++  +l+++l++lf+ +w++k+  vsp ++l++++++ + f+gy+qqDaqEfl+ lld++++el         e+
   Q: 359  gaskgrkmeliqpkepTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQREL---------ET 441
           999888888888877666777************************************************************.........78 PP
   S: 119  eeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkke.......slqdcLesftkeeelege 203
           ++++  +l   +++k  k+  ++++++F+gql sq++cl+cdn+s++ epf+dlsl++p++ +++ +        ++++L++ft++e leg+
   Q: 442  TGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCSGKdiasqpcLVTEMLAKFTETEALEGK 533
           89999999999************************************************9998877788888889***************96 PP
   S: 204  nkyycekckkkqe...........aekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfctspeekqke..kkkYeLia 282
             y c++c++k++           a+k+l++ +lP+vL lhLkRf++s + ++reki  +v f + L+++p+c++++ k+ +  + +Y+L+a
   Q: 534  I-YVCDQCNSKRRrfsskpvvlteAQKQLMICHLPQVLRLHLKRFRWSGR-NNREKIGVHVGFEEILNMEPYCCRETLKSLRpeCFIYDLSA 623
           5.*******999999999999999***********************998.7778********************9988876779******* PP
   S: 283  vvvHsG.slssGHYvayvknekgk.WllfdDskVtevkeeevleetgstaYvlfYkr 337
           vv+H+G +++sGHY+ay++n++g+ W++++Dsk + ++++ev++   ++aY+lfY++
   Q: 624  VVMHHGkGFGSGHYTAYCYNSEGGfWVHCNDSKLSMCTMDEVCK---AQAYILFYTQ 677
           ********************9999********************...********98 PP

Organism

Homo sapiens

Functional Description
(View)

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.

Protein Sequence
(Fasta)

MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH 60
FQESSHPVAL EVNEMYVFCY LCDDYVLNDN TTGDLKLLRR TLSAIKSQNY HCTTRSGRFL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GACTTCAGGA AAGCAGTTTC CAGGAGGTGA GAGTTTAAGC CAAACCTGAA AGAGGTTGAT 60
TTGTAATGAT TTGCAGGGGG GCACTGGAGG CAGCCGCCAG GACTTTTCAC TTAGGAGATC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP

Pfam

PF00443--UCH
PF02148--zf-UBP

SMART

SM00290--ZnF_UBP

Gene Ontology

GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0008270--F:zinc ion binding
GO:0051301--P:cell division
GO:0007067--P:mitotic nuclear division
GO:1904667--P:negative regulation of ubiquitin protein ligase activity
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0016579--P:protein deubiquitination
GO:0090266--P:regulation of mitotic cell cycle spindle assembly checkpoint
GO:0090231--P:regulation of spindle checkpoint

KEGG

hsa:84101

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001220	Ailuropoda melanoleuca	91.16	0.00e+00	1304.00
IUUC-Apl-003673	Anas platyrhynchos	74.79	0.00e+00	1041.00
IUUC-Aca-004434	Anolis carolinensis	73.62	0.00e+00	1002.00
IUUC-Ame-010986	Astyanax mexicanus	64.86	0.00e+00	874.00
IUUC-Bta-012774	Bos taurus	88.64	0.00e+00	1266.00
IUUC-Cja-019619	Callithrix jacchus	93.68	0.00e+00	1324.00
IUUC-Cfa-020957	Canis familiaris	90.04	0.00e+00	1307.00
IUUC-Csa-023177	Chlorocebus sabaeus	94.67	0.00e+00	1335.00
IUUC-Cho-024877	Choloepus hoffmanni	87.18	0.00e+00	828.00
IUUC-Csv-025814	Ciona savignyi	44.16	8.00e-152	530.00
IUUC-Dre-027878	Danio rerio	64.28	0.00e+00	841.00
IUUC-Dno-029832	Dasypus novemcinctus	91.15	0.00e+00	1314.00
IUUC-Ete-032627	Echinops telfairi	86.12	0.00e+00	1247.00
IUUC-Eca-033863	Equus caballus	86.34	0.00e+00	1201.00
IUUC-Eeu-035225	Erinaceus europaeus	85.83	0.00e+00	1189.00
IUUC-Fca-036288	Felis catus	90.88	0.00e+00	1304.00
IUUC-Fal-037207	Ficedula albicollis	74.68	0.00e+00	1000.00
IUUC-Gga-040457	Gallus gallus	73.94	0.00e+00	1011.00
IUUC-Ggo-044926	Gorilla gorilla	98.60	0.00e+00	1390.00
IUUC-Itr-048737	Ictidomys tridecemlineatus	90.45	0.00e+00	1290.00
IUUC-Lch-050260	Latimeria chalumnae	66.57	0.00e+00	913.00
IUUC-Laf-054491	Loxodonta africana	88.41	0.00e+00	1216.00
IUUC-Mcc-055694	Macaca mulatta	94.95	0.00e+00	1334.00
IUUC-Mga-059523	Meleagris gallopavo	73.63	0.00e+00	1023.00
IUUC-Mmr-060885	Microcebus murinus	93.55	0.00e+00	1340.00
IUUC-Mdo-063018	Monodelphis domestica	79.07	0.00e+00	1108.00
IUUC-Mmu-064012	Mus musculus	80.06	0.00e+00	1098.00
IUUC-Mpu-066207	Mustela putorius furo	89.20	0.00e+00	1305.00
IUUC-Mlu-066929	Myotis lucifugus	86.34	0.00e+00	1174.00
IUUC-Nle-069313	Nomascus leucogenys	87.10	0.00e+00	1186.00
IUUC-Opr-070375	Ochotona princeps	81.49	0.00e+00	1116.00
IUUC-Ont-071637	Oreochromis niloticus	63.52	0.00e+00	860.00
IUUC-Oan-073030	Ornithorhynchus anatinus	77.84	0.00e+00	842.00
IUUC-Ocu-074836	Oryctolagus cuniculus	87.59	0.00e+00	1253.00
IUUC-Oga-088367	Otolemur garnettii	92.15	0.00e+00	1299.00
IUUC-Oar-089953	Ovis aries	89.50	0.00e+00	1292.00
IUUC-Ptr-090980	Pan troglodytes	99.02	0.00e+00	1392.00
IUUC-Pan-092748	Papio anubis	95.37	0.00e+00	1338.00
IUUC-Pfo-096860	Poecilia formosa	70.57	5.00e-170	591.00
IUUC-Pab-098514	Pongo abelii	97.34	0.00e+00	1367.00
IUUC-Pva-102984	Pteropus vampyrus	87.94	0.00e+00	1228.00
IUUC-Rno-106019	Rattus norvegicus	80.42	0.00e+00	1134.00
IUUC-Sha-106965	Sarcophilus harrisii	77.70	0.00e+00	1097.00
IUUC-Ssc-115299	Sus scrofa	90.46	0.00e+00	1311.00
IUUC-Tgu-117160	Taeniopygia guttata	73.72	0.00e+00	1001.00
IUUC-Tru-118156	Takifugu rubripes	60.82	0.00e+00	825.00
IUUC-Tni-119733	Tetraodon nigroviridis	61.64	0.00e+00	848.00
IUUC-Tbe-128084	Tupaia belangeri	85.81	0.00e+00	1212.00
IUUC-Ttr-128928	Tursiops truncatus	91.30	0.00e+00	1322.00
IUUC-Vpa-130706	Vicugna pacos	90.88	0.00e+00	1303.00
IUUC-Xtr-132061	Xenopus tropicalis	71.54	0.00e+00	926.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence