iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046147

UUCD1 version

UUC-HoS-00123

Ensembl Protein ID

ENSP00000283943.4

UniProt Accession

Q14669; D4HL82; Q14CA3; Q14CF1; Q15644; Q53R87; Q53TE7; TRIPC_HUMAN

Genbank Protein ID

ACC99349.1; BAA05837.2; AAY14755.1; AAY14681.1; AAI14557.1; AAI13892.1; AAC41731.1

Protein Name

E3 ubiquitin-protein ligase TRIP12; E3 ubiquitin-protein ligase for Arf; HECT-type E3 ubiquitin transferase TRIP12; Thyroid receptor-interacting protein 12

Genbank Nucleotide ID

EU489742; D28476; AC009973; AC093384; AC105380; BC113891; L40383

Gene Name

TRIP12; KIAA0045; ULF

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000153827.13	ENST00000283943.9	ENSP00000283943.4
ENSG00000153827.13	ENST00000389045.7	ENSP00000373697.3
ENSG00000153827.13	ENST00000389044.8	ENSP00000373696.4
ENSG00000153827.13	ENST00000418123.1	ENSP00000408330.1
ENSG00000153827.13	ENST00000409677.5	ENSP00000386388.1
ENSG00000153827.13	ENST00000453485.1	ENSP00000400967.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	TargetScan	RepTar	miRNAMap
SomamiR	miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Disease-associated Information
ClinVar	GWASdb	GWAS Central	OMIM
Drug and target
TTD
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	20208519; 20829358; 20175895; 18627766

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	3.60e-96	324.1	1613	1992

Active Site

Position(s)	Description	Evidence
1959	Glycyl thioester intermediate	{ECO:0000305}

Domain Profile

E3 activity/HECT

   S: 6    kkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlq..pnpdsglknp.....................ehlklFe 72
           ++ le+++++E g++l G+t+ef+al+s+elq++dlgl++ +e   ++ +   + +++                         ++   F+
   Q: 1613 RAMLEIQYENEVGTGL-GPTLEFYALVSQELQRADLGLWRGEEVTLSNPKgsQEGTKY---IqnlqglfalpfgrtakpahiaKVKMKFR 1698
           56899***********.**********************6665533332200222333...14458888999**********9999**** PP
   S: 73   flGrllgkalqdgrlldlplsksfyklllgkklsl..edleevdpelykslkeikena......es.....................die 133
           flG+l++ka++d rl+dlpl  +fyk +l+++ sl  +dl ++dp +++s+  +++ +      e+                     ++e
   Q: 1699 FLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLtsHDLFDIDPVVARSVYHLEDIVrqkkrlEQdksqtkeslqyaletltmngcSVE 1788
           *******************************654444**************999999855555511555666666554444444444444 PP
   S: 134  elgldltfsvseei.....lgetkeeeLkpgGenipVtneNkeeYvklvveyrlkegikeqveafreGfneviplellslFteeelelli 218
           +            +     l+   + eLk gG++ipVt+ N+eeY +lv+ + l+eg+ +q+++fr+Gf++v+pl++l++F +eel++l+
   Q: 1789 D------------LgldftLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLL 1866
           4............25555578999****************************************************************** PP
   S: 219  cGe..eeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlgGfaelkprlfvirk.....dasddrlPss 301
           cG+  +++d+++l + ++ ++gyt+ds+a+++++e+l++f++e+++ fLqFvTgs+rlP+gGf++l+p l+++rk     ++ dd lPs+
   Q: 1867 CGSkaDTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKtfestENPDDFLPSV 1956
           ***7778999999999999***********************************************************988888****** PP
   S: 302  nTCfnyLkLPeYsskeilkekLlyAieet.kgFels 336
           +TC+nyLkLP+Yss ei++ekLl+A +e+ ++F+ls
   Q: 1957 MTCVNYLKLPDYSSIEIMREKLLIAAREGqQSFHLS 1992
           *********************************985 PP

Organism

Homo sapiens

Functional Description
(View)

E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.

Protein Sequence
(Fasta)

MSNRPNNNPG GSLRRSQRNT AGAQPQDDSI GGRSCSSSSA VIVPQPEDPD RANTSERQKT 60
GQVPKKDNSR GVKRSASPDY NRTNSPSSAK KPKALQHTES PSETNKPHSK SKKRHLDQEQ 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GTCATTATGG GTGGGGCTGA AGCGCAGAGC AGCTGAAACA TTTTTATACC ACCACCCGCA 60
CACTTTCAAT GGGATAGAAG GGACTCCAAA AGGTAAGAGC TCCCAATCGT CAGTTGCTAC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR000569--HECT_dom
IPR004170--WWE-dom

PROSITE

PS50237--HECT
PS50918--WWE

Pfam

PF00632--HECT

SMART

SM00119--HECTc

Gene Ontology

GO:0005829--C:cytosol
GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0046966--F:thyroid hormone receptor binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006974--P:cellular response to DNA damage stimulus
GO:0006281--P:DNA repair
GO:0009790--P:embryo development
GO:2000780--P:negative regulation of double-strand break repair
GO:1901315--P:negative regulation of histone H2A K63-linked ubiquitination
GO:0000209--P:protein polyubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process

KEGG

hsa:9320

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000750	Aegilops tauschii	38.69	2.00e-126	447.00
IUUC-Aml-001502	Ailuropoda melanoleuca	95.83	0.00e+00	3625.00
IUUC-Atr-002821	Amborella trichopoda	37.45	7.00e-117	416.00
IUUC-Apl-003531	Anas platyrhynchos	91.04	0.00e+00	3359.00
IUUC-Aly-005962	Arabidopsis lyrata	38.94	8.00e-115	409.00
IUUC-Ath-006636	Arabidopsis thaliana	38.66	5.00e-114	406.00
IUUC-Ago-007875	Ashbya gossypii	36.20	1.00e-81	299.00
IUUC-Acl-008242	Aspergillus clavatus	35.70	2.00e-113	404.00
IUUC-Afl-008736	Aspergillus flavus	38.83	2.00e-110	395.00
IUUC-Afu-008812	Aspergillus fumigatus	35.10	5.00e-114	406.00
IUUC-Ani-009407	Aspergillus nidulans	36.82	3.00e-110	394.00
IUUC-Ang-009763	Aspergillus niger	40.00	4.00e-106	380.00
IUUC-Aor-009941	Aspergillus oryzae	30.77	1.00e-112	401.00
IUUC-Ate-010459	Aspergillus terreus	36.42	1.00e-85	312.00
IUUC-Ame-011761	Astyanax mexicanus	85.37	0.00e+00	2919.00
IUUC-Bgr-012265	Blumeria graminis	34.86	4.00e-104	374.00
IUUC-Bta-013070	Bos taurus	98.34	0.00e+00	3647.00
IUUC-Bci-013890	Botrytis cinerea	39.72	2.00e-102	368.00
IUUC-Bdi-014046	Brachypodium distachyon	38.47	6.00e-122	433.00
IUUC-Bol-015942	Brassica oleracea	44.23	1.00e-114	408.00
IUUC-Bra-016849	Brassica rapa	44.23	4.00e-114	407.00
IUUC-Cja-019046	Callithrix jacchus	96.43	0.00e+00	3600.00
IUUC-Cfa-020521	Canis familiaris	94.95	0.00e+00	3486.00
IUUC-Cpo-021639	Cavia porcellus	91.63	0.00e+00	3501.00
IUUC-Cre-022767	Chlamydomonas reinhardtii	39.88	1.00e-89	323.00
IUUC-Csa-023231	Chlorocebus sabaeus	95.36	0.00e+00	3596.00
IUUC-Cho-024380	Choloepus hoffmanni	88.73	0.00e+00	3264.00
IUUC-Cin-025482	Ciona intestinalis	51.21	7.00e-160	557.00
IUUC-Csv-026121	Ciona savignyi	49.47	0.00e+00	1562.00
IUUC-Cgl-026716	Colletotrichum gloeosporioides	34.98	1.00e-110	395.00
IUUC-Cne-026857	Cryptococcus neoformans	39.89	2.00e-94	342.00
IUUC-Cme-027226	Cyanidioschyzon merolae	35.14	4.00e-74	274.00
IUUC-Dre-028174	Danio rerio	85.28	0.00e+00	2967.00
IUUC-Dor-030939	Dipodomys ordii	88.27	0.00e+00	2789.00
IUUC-Dse-031444	Dothistroma septosporum	39.31	2.00e-105	378.00
IUUC-Dme-032162	Drosophila melanogaster	63.41	5.00e-158	553.00
IUUC-Ete-032345	Echinops telfairi	92.38	0.00e+00	2308.00
IUUC-Eca-033229	Equus caballus	95.25	0.00e+00	3537.00
IUUC-Eeu-035205	Erinaceus europaeus	88.76	0.00e+00	3152.00
IUUC-Fca-036444	Felis catus	95.05	0.00e+00	3569.00
IUUC-Fal-037131	Ficedula albicollis	90.28	0.00e+00	3402.00
IUUC-Fox-038035	Fusarium oxysporum	38.54	4.00e-110	392.00
IUUC-Fso-038320	Fusarium solani	36.91	6.00e-110	393.00
IUUC-Gmo-039497	Gadus morhua	82.75	0.00e+00	3109.00
IUUC-Ggr-040057	Gaeumannomyces graminis	34.90	3.00e-108	387.00
IUUC-Gga-041021	Gallus gallus	90.28	0.00e+00	3408.00
IUUC-Gac-042474	Gasterosteus aculeatus	84.18	0.00e+00	3090.00
IUUC-Gma-043060	Glycine max	38.92	4.00e-122	433.00
IUUC-Ggo-044855	Gorilla gorilla	96.67	0.00e+00	3609.00
IUUC-Hvu-047775	Hordeum vulgare	38.69	7.00e-125	442.00
IUUC-Itr-048131	Ictidomys tridecemlineatus	96.35	0.00e+00	3499.00
IUUC-Kpa-049098	Komagataella pastoris	38.10	4.00e-86	313.00
IUUC-Lch-049818	Latimeria chalumnae	84.29	0.00e+00	3070.00
IUUC-Lpe-051017	Leersia perrieri	38.44	2.00e-118	421.00
IUUC-Loc-051777	Lepisosteus oculatus	84.68	0.00e+00	3279.00
IUUC-Lma-052990	Leptosphaeria maculans	39.74	6.00e-112	400.00
IUUC-Laf-053727	Loxodonta africana	95.44	0.00e+00	3578.00
IUUC-Mcc-055799	Macaca mulatta	93.90	0.00e+00	3505.00
IUUC-Meu-056065	Macropus eugenii	93.88	0.00e+00	2103.00
IUUC-Mor-057054	Magnaporthe oryzae	38.86	2.00e-107	384.00
IUUC-Mpo-057417	Magnaporthe poae	39.55	1.00e-109	392.00
IUUC-Mtr-057849	Medicago truncatula	37.36	1.00e-113	405.00
IUUC-Mla-058870	Melampsora laricipopulina	42.57	7.00e-107	382.00
IUUC-Mga-059580	Meleagris gallopavo	90.92	0.00e+00	2956.00
IUUC-Mvi-060507	Microbotryum violaceum	40.19	9.00e-113	402.00
IUUC-Mmr-060816	Microcebus murinus	94.54	0.00e+00	3565.00
IUUC-Mdo-062866	Monodelphis domestica	90.62	0.00e+00	3273.00
IUUC-Mmu-063906	Mus musculus	95.75	0.00e+00	3563.00
IUUC-Mac-064675	Musa acuminata	38.92	2.00e-111	396.00
IUUC-Mpu-066199	Mustela putorius furo	92.79	0.00e+00	3461.00
IUUC-Mlu-067110	Myotis lucifugus	95.90	0.00e+00	3558.00
IUUC-Nfi-068482	Neosartorya fischeri	35.25	6.00e-114	406.00
IUUC-Ncr-068906	Neurospora crassa	39.13	3.00e-109	391.00
IUUC-Nle-069410	Nomascus leucogenys	96.32	0.00e+00	3594.00
IUUC-Opr-070401	Ochotona princeps	86.29	0.00e+00	3018.00
IUUC-Ont-072100	Oreochromis niloticus	79.92	0.00e+00	3046.00
IUUC-Oan-073121	Ornithorhynchus anatinus	90.72	0.00e+00	3530.00
IUUC-Ocu-074623	Oryctolagus cuniculus	92.79	0.00e+00	3494.00
IUUC-Oba-075380	Oryza barthii	36.51	2.00e-113	404.00
IUUC-Obr-076915	Oryza brachyantha	37.77	7.00e-119	422.00
IUUC-Ogl-077055	Oryza glaberrima	38.70	3.00e-118	421.00
IUUC-Ogu-078892	Oryza glumaepatula	40.95	1.00e-112	401.00
IUUC-Oin-079256	Oryza indica	38.52	5.00e-117	416.00
IUUC-Olo-080343	Oryza longistaminata	38.52	2.00e-117	418.00
IUUC-Ome-081422	Oryza meridionalis	38.24	5.00e-117	416.00
IUUC-Oni-083047	Oryza nivara	41.13	1.00e-114	408.00
IUUC-Opu-083641	Oryza punctata	36.44	4.00e-111	396.00
IUUC-Oru-084439	Oryza rufipogon	40.95	3.00e-113	403.00
IUUC-Osa-085972	Oryza sativa	38.44	2.00e-116	413.00
IUUC-Ola-087173	Oryzias latipes	80.43	0.00e+00	3029.00
IUUC-Olu-087871	Ostreococcus lucimarinus	36.89	8.00e-89	321.00
IUUC-Oga-087908	Otolemur garnettii	95.55	0.00e+00	3579.00
IUUC-Oar-090277	Ovis aries	95.06	0.00e+00	3604.00
IUUC-Ptr-091543	Pan troglodytes	99.90	0.00e+00	3645.00
IUUC-Pan-092243	Papio anubis	93.86	0.00e+00	3502.00
IUUC-Psi-093150	Pelodiscus sinensis	92.36	0.00e+00	3526.00
IUUC-Pno-094891	Phaeosphaeria nodorum	40.94	4.00e-113	404.00
IUUC-Ppa-095181	Physcomitrella patens	42.14	2.00e-107	383.00
IUUC-Pfo-096267	Poecilia formosa	79.35	0.00e+00	3021.00
IUUC-Pab-098072	Pongo abelii	96.62	0.00e+00	3606.00
IUUC-Pop-099190	Populus trichocarpa	41.34	4.00e-116	414.00
IUUC-Pca-100311	Procavia capensis	90.83	0.00e+00	3296.00
IUUC-Ppe-101284	Prunus persica	37.52	3.00e-116	414.00
IUUC-Pva-102215	Pteropus vampyrus	90.29	0.00e+00	1298.00
IUUC-Pgr-103694	Puccinia graminis	40.68	3.00e-104	374.00
IUUC-Ptt-103916	Puccinia triticina	39.00	2.00e-98	355.00
IUUC-Pte-104346	Pyrenophora teres	39.59	3.00e-110	394.00
IUUC-Pyt-104508	Pyrenophora triticirepentis	39.66	2.00e-110	394.00
IUUC-Rno-105315	Rattus norvegicus	92.55	0.00e+00	3422.00
IUUC-Sce-106273	Saccharomyces cerevisiae	35.00	6.00e-71	263.00
IUUC-Sha-106835	Sarcophilus harrisii	92.31	0.00e+00	3557.00
IUUC-Sja-107943	Schizosaccharomyces japonicus	37.58	1.00e-94	342.00
IUUC-Spo-108081	Schizosaccharomyces pombe	38.54	2.00e-94	342.00
IUUC-Ssl-108560	Sclerotinia sclerotiorum	39.92	4.00e-106	380.00
IUUC-Smo-109590	Selaginella moellendorffii	43.28	4.00e-112	400.00
IUUC-Sit-110717	Setaria italica	37.94	5.00e-120	426.00
IUUC-Sly-111469	Solanum lycopersicum	39.08	1.00e-121	432.00
IUUC-Stu-112896	Solanum tuberosum	38.22	1.00e-105	377.00
IUUC-Sar-113224	Sorex araneus	92.70	0.00e+00	1952.00
IUUC-Sbi-114313	Sorghum bicolor	38.02	1.00e-123	438.00
IUUC-Sre-114993	Sporisorium reilianum	34.09	2.00e-100	362.00
IUUC-Ssc-115535	Sus scrofa	93.16	0.00e+00	3456.00
IUUC-Tgu-117493	Taeniopygia guttata	90.62	0.00e+00	2689.00
IUUC-Tru-118669	Takifugu rubripes	80.23	0.00e+00	3007.00
IUUC-Tsy-119373	Tarsius syrichta	94.75	0.00e+00	1869.00
IUUC-Tni-120049	Tetraodon nigroviridis	77.98	0.00e+00	867.00
IUUC-Tca-121779	Theobroma cacao	38.76	1.00e-115	412.00
IUUC-Tre-122195	Trichoderma reesei	35.80	2.00e-105	378.00
IUUC-Tvi-122650	Trichoderma virens	35.93	4.00e-109	390.00
IUUC-Tae-123580	Triticum aestivum	38.58	2.00e-124	440.00
IUUC-Tur-126295	Triticum urartu	38.54	2.00e-125	444.00
IUUC-Tme-126964	Tuber melanosporum	40.48	1.00e-105	378.00
IUUC-Tbe-128149	Tupaia belangeri	81.95	0.00e+00	1467.00
IUUC-Ttr-128165	Tursiops truncatus	94.81	0.00e+00	3063.00
IUUC-Uma-129365	Ustilago maydis	36.07	7.00e-89	323.00
IUUC-Vda-129725	Verticillium dahliae	41.08	6.00e-109	389.00
IUUC-Vpa-130678	Vicugna pacos	98.91	0.00e+00	2692.00
IUUC-Vvi-131147	Vitis vinifera	39.23	2.00e-122	434.00
IUUC-Xtr-132636	Xenopus tropicalis	87.43	0.00e+00	3265.00
IUUC-Xma-133458	Xiphophorus maculatus	79.87	0.00e+00	3061.00
IUUC-Yli-134481	Yarrowia lipolytica	39.56	7.00e-98	352.00
IUUC-Zma-135782	Zea mays	37.87	6.00e-118	419.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence