iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	circBase	circRNADb	CircNet
TRANSFAC	TargetScan	RepTar	miRcode
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Disease-associated Information
OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
UniProt
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	20133869

Classification

Family	E-value	Score	Start	End
E1	8.60e-222	738.3	415	890

Active Site

Position(s)	Description	Evidence
599	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGv.....gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpd 87
           YD+Qi+++G+  qekl+++++llvGag++GcEllK ++l+G+     g +tvvDmd++e snl+rqFLfr +dvg++KAevaa+a++ lnpd
   Q: 415  YDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLgagnsGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPD 506
           ******************************************************************************************** PP
   S: 88   vnveaheesiteleeeiv...FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPf 173
            +v + ++++++++e+i+   Ff+++d+V++ald+++arr+v+ +c+++ +pl+e+gt G++G ++v+++++te+y+++     +++  +P+
   Q: 507  LQVIPLTYPLDPTTEHIYgdnFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPAsaaASEDAPYPV 598
           *********************************************************************************999******** PP
   S: 174  ctlketpnaaehtiewa........vlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttinsikqll 257
           ct++ +p++aeht++wa        +l ++++++++++++ l+++d+ ++++ +++v   l+  +++n+++++++a+ +++ +++++ikqll
   Q: 599  CTVRYFPSTAEHTLQWArhefeelfRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRV-RPQNWQDCVAWALGHWKLCFHYGIKQLL 689
           *************************9*******************************888777.6*************************** PP
   S: 258  ksfecdivtkskspfwv................................................sfcsnaealqvpekvekdeevvkasap 301
           ++f++++v ++++pfw+                                                ++ ++ + +q+ +  +++ e  +asa+
   Q: 690  RHFPPNKVLEDGTPFWSgpkqcpqplefdtnqdthllyvlaaanlyaqmhglpgsqdwtalrellKLLPQPDPQQMAPIFASNLELASASAE 781
           *******************************************************************************************9 PP
   S: 302  .....lylllralerfekkkgrkpgelsfekddds..avdlvtaaanlraeslgiepkldddlikeiagniipaiattnavvgGlaaqEviK 386
                ++ l +ale ++  + +kp  l+fekddds  +vd+v aaa+lr++++gi+p +++ + k+i+g+iipaiatt+a+v+Gl+ +E++K
   Q: 782  fgpeqQKELNKALEVWSVGPPLKP--LMFEKDDDSnfHVDFVVAAASLRCQNYGIPP-VNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYK 870
           ***********************9..*******************************.********************************** PP
   S: 387  vvsg..kfeplknfflfdae 404
           vvsg  + +++++ +l++ae
   Q: 871  VVSGprPRSAFRHSYLHLAE 890
           ****8777889998888775 PP

Organism

Homo sapiens

Functional Description
(View)

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Protein Sequence
(Fasta)

MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN LVLMGVGSLT 60
LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR AVQVVVHTGD ITEDLLLDFQ 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CCAGGAAGAG AGCTGTGACC AGCAGCGTCC CTTATTCGCT TGGCCTTGGT TCCTGTTTGC 60
ACTGGCTACA GCAGGGCACT GGCCCCTACT GTCACCGCCA CCTACACAAA GACCCTATCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR032420--E1_4HB
IPR032418--E1_FCCH
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR018965--Ub-activating_enz_E1_C
IPR019572--UBA_E1_Cys
IPR018075--UBQ-activ_enz_E1
IPR033127--UBQ-activ_enz_E1_Cys_AS
IPR000011--UBQ/SUMO-activ_enz_E1-like

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF16191--E1_4HB
PF16190--E1_FCCH
PF09358--E1_UFD
PF00899--ThiF
PF10585--UBA_e1_thiolCys

PRINTS

PR01849--UBIQUITINACT

SMART

SM00985--UBA_e1_C

Gene Ontology

GO:0005829--C:cytosol
GO:0005654--C:nucleoplasm
GO:0005524--F:ATP binding
GO:0019782--F:ISG15 activating enzyme activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006464--P:cellular protein modification process
GO:0032020--P:ISG15-protein conjugation
GO:0019941--P:modification-dependent protein catabolic process
GO:0032480--P:negative regulation of type I interferon production
GO:0019985--P:translesion synthesis

KEGG

hsa:7318

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001816	Ailuropoda melanoleuca	79.58	0.00e+00	1461.00
IUUC-Bta-012665	Bos taurus	76.19	0.00e+00	1357.00
IUUC-Cja-019047	Callithrix jacchus	90.02	0.00e+00	1703.00
IUUC-Cfa-021225	Canis familiaris	80.83	0.00e+00	1536.00
IUUC-Cpo-022336	Cavia porcellus	71.32	0.00e+00	1320.00
IUUC-Csa-023462	Chlorocebus sabaeus	96.05	0.00e+00	1746.00
IUUC-Cho-024436	Choloepus hoffmanni	76.37	4.00e-93	336.00
IUUC-Dno-029347	Dasypus novemcinctus	79.84	0.00e+00	1477.00
IUUC-Dor-030997	Dipodomys ordii	75.00	0.00e+00	1366.00
IUUC-Ete-033124	Echinops telfairi	73.82	0.00e+00	926.00
IUUC-Eca-033929	Equus caballus	81.92	0.00e+00	1559.00
IUUC-Eeu-034717	Erinaceus europaeus	68.64	0.00e+00	944.00
IUUC-Fca-036564	Felis catus	81.95	0.00e+00	1506.00
IUUC-Ggo-044480	Gorilla gorilla	98.50	0.00e+00	1811.00
IUUC-Itr-048657	Ictidomys tridecemlineatus	78.34	0.00e+00	1439.00
IUUC-Laf-054119	Loxodonta africana	78.75	0.00e+00	1502.00
IUUC-Mcc-055597	Macaca mulatta	93.98	0.00e+00	1678.00
IUUC-Meu-056534	Macropus eugenii	49.35	0.00e+00	877.00
IUUC-Mdo-063058	Monodelphis domestica	56.85	0.00e+00	1017.00
IUUC-Mmu-063578	Mus musculus	73.43	0.00e+00	1393.00
IUUC-Mpu-066255	Mustela putorius furo	79.68	0.00e+00	1501.00
IUUC-Mlu-067814	Myotis lucifugus	76.32	0.00e+00	1357.00
IUUC-Nle-069193	Nomascus leucogenys	95.95	0.00e+00	1768.00
IUUC-Opr-070715	Ochotona princeps	73.42	0.00e+00	1382.00
IUUC-Ocu-074162	Oryctolagus cuniculus	77.89	0.00e+00	1455.00
IUUC-Oga-088895	Otolemur garnettii	80.04	0.00e+00	1475.00
IUUC-Oar-089579	Ovis aries	75.79	0.00e+00	1347.00
IUUC-Ptr-090581	Pan troglodytes	99.19	0.00e+00	1790.00
IUUC-Pan-092458	Papio anubis	96.15	0.00e+00	1743.00
IUUC-Pab-098653	Pongo abelii	91.30	0.00e+00	1658.00
IUUC-Pca-100578	Procavia capensis	76.02	0.00e+00	878.00
IUUC-Pva-102914	Pteropus vampyrus	79.31	0.00e+00	1176.00
IUUC-Rno-106066	Rattus norvegicus	73.21	0.00e+00	1404.00
IUUC-Sha-107368	Sarcophilus harrisii	59.62	0.00e+00	1103.00
IUUC-Ssc-115743	Sus scrofa	81.72	0.00e+00	1504.00
IUUC-Tbe-127587	Tupaia belangeri	74.25	0.00e+00	1204.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence