iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000028556.5	ENSRNOT00000086231.1	ENSRNOP00000072669.1
ENSRNOG00000028556.5	ENSRNOT00000030891.4	ENSRNOP00000034698.4

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
TRANSFAC	RAID2
Protein-protein Interaction
IID
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	4.50e-71	240.2	69	501

Active Site

Position(s)	Description	Evidence
77	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
452	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelw...kskkkavsprellkalkkeaeefsgy 87
           Gl NlgNtC+mNS+LQ Ls++p + + l e +++  ++++ ++++++l+ +l +l+++l     +++++ + + ll++l+++  ++s++
   Q: 69   GLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLSLLKALScqeVTEEEVLDASCLLDVLRMYRWQISSF 157
           9*****************************6666666666688999*************888899999********************* PP
   S: 88   eqqDaqEflaflldqleeelkkeknkpkeeeeeedkselsde..........aeekkkkknkslisdlFqgqlksqikclecdnvs.sr 165
           e+qDa+E+++++ ++le+e    ++ +++++++ ++ e+++e          + + +++ n+   ++ F+g+l+s+++c++c+++s +r
   Q: 158  EEQDAHELFHVITSSLEDER--DRQPRVTHLFDVHSLEQQSEmaprqvtchtRGSPHPTTNPWKSQHPFHGRLSSNMVCKHCEHQSpVR 244
           *******************9..66888889999988888888899999999999999******************************** PP
   S: 166  eepfldlslpvpgkeetkkeslqdcLesftkeeelegenkyycekck...............kkqeaekklklkklPevLilhLkRfsf 239
           +++f +lsl++p  +  ++++l++cL++f + e++++   + c++c+               ++++ +k+lkl klP++L++hL+R s+
   Q: 245  FDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRD---VVCDNCTkieakgtqngekvehQRTTFVKQLKLGKLPQCLCIHLQRLSW 330
           ***********************************97...9******8888888888888777777*********************** PP
   S: 240  seeqksrekinkkvefpleLdlspfcts.........................................peekqke............. 274
           s++ + + k +++v+f++ L ++ +  +                                         +     +             
   Q: 331  SSQ-GTPLKRHEHVQFNEFLMMDFYKYRllghkpsqhgpkatespgsalgvqdtqaapkpglsqpaapkT-----Qffmngacspsllp 413
           996.8889*********99999866666777787777777777777776666666666655555555550.....05555555555555 PP
   S: 275  ...................kkkYeLiavvvHsGslssGHYvayvkn.........ekgkWllfdDskVtevkeeevleetgstaYvlfY 335
                               + ++L+avvvH+G ++sGH+v+y ++         ++++Wl ++D++V++ + +evl+   s+aY+lfY
   Q: 414  alpspmafplpvapdysssMYLFRLMAVVVHHGDMHSGHFVTYRRSppsaknplsTSNQWLWISDDTVRKASLQEVLS---SSAYLLFY 499
           55555555555555555559999***********************9999888888999*******************...******** PP
   S: 336  kr 337
           +r
   Q: 500  ER 501
           98 PP

Organism

Rattus norvegicus

Functional Description
(View)

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:24896179). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Protein Sequence
(Fasta)

MLSSRAQAAR TAADKALQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY VIWGPITERK 60
KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFVKWLEEFT TQYSRDQQGP HTHQCLSLTL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATTGGCAAAC TCCTTGGGGA CAGCTGCTCC ATCATCCAGC TCGACAGCTT CTAGGCCCAC 60
TCGGACCCCC TCAATCTGAA CATCATGTTC CCCGGAAGCC GCGTCGTCCT CTGACCGTGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0378--Hydrolase
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH

Gene Ontology

GO:0016021--C:integral component of membrane
GO:0005741--C:mitochondrial outer membrane
GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008053--P:mitochondrial fusion
GO:0000422--P:mitophagy
GO:0035871--P:protein K11-linked deubiquitination
GO:0044313--P:protein K6-linked deubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

rno:304579

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000204	Aegilops tauschii	28.96	2.00e-16	80.50
IUUC-Aml-001733	Ailuropoda melanoleuca	88.27	0.00e+00	828.00
IUUC-Atr-002510	Amborella trichopoda	25.58	9.00e-21	95.10
IUUC-Apl-003645	Anas platyrhynchos	72.56	6.00e-108	383.00
IUUC-Aca-004583	Anolis carolinensis	68.42	3.00e-24	102.00
IUUC-Aly-005754	Arabidopsis lyrata	25.87	1.00e-24	108.00
IUUC-Ath-007622	Arabidopsis thaliana	25.44	6.00e-22	99.00
IUUC-Ame-010896	Astyanax mexicanus	62.26	6.00e-154	537.00
IUUC-Bgr-012133	Blumeria graminis	21.38	1.00e-09	58.50
IUUC-Bta-012321	Bos taurus	87.23	0.00e+00	817.00
IUUC-Bci-013590	Botrytis cinerea	22.31	3.00e-14	73.90
IUUC-Bdi-014795	Brachypodium distachyon	27.97	2.00e-16	80.10
IUUC-Bol-015637	Brassica oleracea	25.62	2.00e-23	103.00
IUUC-Bra-016801	Brassica rapa	26.76	4.00e-23	102.00
IUUC-Cel-018308	Caenorhabditis elegans	29.44	4.00e-38	152.00
IUUC-Cja-018826	Callithrix jacchus	88.61	0.00e+00	879.00
IUUC-Cfa-021206	Canis familiaris	87.12	0.00e+00	838.00
IUUC-Cpo-021343	Cavia porcellus	88.02	0.00e+00	823.00
IUUC-Csa-023419	Chlorocebus sabaeus	88.39	0.00e+00	879.00
IUUC-Cho-024475	Choloepus hoffmanni	84.73	9.00e-130	456.00
IUUC-Cgl-026621	Colletotrichum gloeosporioides	21.39	1.00e-13	71.60
IUUC-Cne-027061	Cryptococcus neoformans	25.63	4.00e-09	56.60
IUUC-Dre-027563	Danio rerio	58.45	3.00e-156	544.00
IUUC-Dno-029929	Dasypus novemcinctus	87.04	0.00e+00	849.00
IUUC-Dor-030166	Dipodomys ordii	80.27	0.00e+00	725.00
IUUC-Dme-031627	Drosophila melanogaster	34.27	3.00e-36	146.00
IUUC-Ete-032634	Echinops telfairi	78.34	0.00e+00	667.00
IUUC-Eca-033968	Equus caballus	88.57	0.00e+00	798.00
IUUC-Eeu-035279	Erinaceus europaeus	80.49	9.00e-57	214.00
IUUC-Fca-036525	Felis catus	88.97	0.00e+00	858.00
IUUC-Fal-036606	Ficedula albicollis	73.33	1.00e-156	545.00
IUUC-Gmo-038726	Gadus morhua	56.16	8.00e-145	506.00
IUUC-Gga-040718	Gallus gallus	72.68	1.00e-158	552.00
IUUC-Gac-042645	Gasterosteus aculeatus	57.62	3.00e-153	534.00
IUUC-Gma-042890	Glycine max	24.95	1.00e-22	101.00
IUUC-Ggo-045021	Gorilla gorilla	88.78	0.00e+00	878.00
IUUC-Hsa-045832	Homo sapiens	88.78	0.00e+00	878.00
IUUC-Itr-048392	Ictidomys tridecemlineatus	91.02	0.00e+00	828.00
IUUC-Lch-050662	Latimeria chalumnae	69.92	1.00e-176	612.00
IUUC-Lpe-051078	Leersia perrieri	26.55	3.00e-29	123.00
IUUC-Loc-052611	Lepisosteus oculatus	65.44	2.00e-179	622.00
IUUC-Lma-053048	Leptosphaeria maculans	19.92	6.00e-15	76.30
IUUC-Laf-053752	Loxodonta africana	87.76	0.00e+00	793.00
IUUC-Mcc-054892	Macaca mulatta	88.78	0.00e+00	881.00
IUUC-Meu-056673	Macropus eugenii	72.58	3.00e-159	554.00
IUUC-Mor-057143	Magnaporthe oryzae	20.55	2.00e-09	58.20
IUUC-Mtr-057976	Medicago truncatula	24.13	4.00e-29	122.00
IUUC-Mga-059543	Meleagris gallopavo	74.39	0.00e+00	651.00
IUUC-Mmr-061534	Microcebus murinus	88.59	0.00e+00	850.00
IUUC-Mdo-062140	Monodelphis domestica	67.13	5.00e-162	564.00
IUUC-Mmu-063673	Mus musculus	96.32	0.00e+00	896.00
IUUC-Mpu-066328	Mustela putorius furo	88.20	0.00e+00	824.00
IUUC-Mlu-067938	Myotis lucifugus	88.39	0.00e+00	862.00
IUUC-Nle-069426	Nomascus leucogenys	86.88	0.00e+00	778.00
IUUC-Opr-070796	Ochotona princeps	83.66	4.00e-119	421.00
IUUC-Ont-072248	Oreochromis niloticus	58.25	1.00e-156	545.00
IUUC-Oan-072916	Ornithorhynchus anatinus	78.36	0.00e+00	743.00
IUUC-Ocu-074459	Oryctolagus cuniculus	84.54	0.00e+00	646.00
IUUC-Oba-075765	Oryza barthii	27.38	1.00e-28	120.00
IUUC-Obr-076005	Oryza brachyantha	27.38	1.00e-29	124.00
IUUC-Ogl-077916	Oryza glaberrima	28.02	2.00e-32	133.00
IUUC-Ogu-078263	Oryza glumaepatula	27.77	4.00e-29	122.00
IUUC-Oin-080096	Oryza indica	28.23	4.00e-23	102.00
IUUC-Ome-081928	Oryza meridionalis	27.18	3.00e-30	126.00
IUUC-Oni-082424	Oryza nivara	27.98	4.00e-29	122.00
IUUC-Oru-085117	Oryza rufipogon	27.98	4.00e-29	122.00
IUUC-Ola-087429	Oryzias latipes	55.58	6.00e-148	517.00
IUUC-Oga-087981	Otolemur garnettii	87.81	0.00e+00	837.00
IUUC-Oar-089927	Ovis aries	87.62	0.00e+00	820.00
IUUC-Ptr-090878	Pan troglodytes	88.78	0.00e+00	878.00
IUUC-Pan-092698	Papio anubis	88.78	0.00e+00	881.00
IUUC-Psi-093451	Pelodiscus sinensis	75.81	0.00e+00	704.00
IUUC-Ppa-096015	Physcomitrella patens	23.10	2.00e-12	67.80
IUUC-Pfo-096096	Poecilia formosa	60.00	2.00e-155	541.00
IUUC-Pab-098676	Pongo abelii	87.43	0.00e+00	857.00
IUUC-Pca-100418	Procavia capensis	91.19	1.00e-123	436.00
IUUC-Ppe-101898	Prunus persica	24.67	3.00e-23	103.00
IUUC-Pva-103018	Pteropus vampyrus	86.07	0.00e+00	826.00
IUUC-Pgr-103423	Puccinia graminis	26.97	3.00e-10	61.20
IUUC-Pte-104330	Pyrenophora teres	20.92	2.00e-13	70.90
IUUC-Pyt-104518	Pyrenophora triticirepentis	20.66	3.00e-15	77.40
IUUC-Sha-106971	Sarcophilus harrisii	79.24	0.00e+00	751.00
IUUC-Ssl-108648	Sclerotinia sclerotiorum	24.37	6.00e-14	72.80
IUUC-Sit-110442	Setaria italica	27.52	4.00e-26	112.00
IUUC-Ssc-116397	Sus scrofa	87.43	0.00e+00	821.00
IUUC-Tgu-117446	Taeniopygia guttata	68.74	0.00e+00	632.00
IUUC-Tru-118268	Takifugu rubripes	56.32	2.00e-143	502.00
IUUC-Tsy-119484	Tarsius syrichta	87.40	8.00e-51	194.00
IUUC-Tni-119735	Tetraodon nigroviridis	56.10	7.00e-125	440.00
IUUC-Tca-121660	Theobroma cacao	24.95	4.00e-26	112.00
IUUC-Tae-125383	Triticum aestivum	28.51	4.00e-28	119.00
IUUC-Tur-126458	Triticum urartu	27.64	1.00e-25	110.00
IUUC-Tbe-127740	Tupaia belangeri	82.59	0.00e+00	760.00
IUUC-Ttr-129273	Tursiops truncatus	86.07	0.00e+00	801.00
IUUC-Vda-129789	Verticillium dahliae	23.04	9.00e-09	55.80
IUUC-Vvi-131177	Vitis vinifera	24.71	8.00e-25	108.00
IUUC-Xtr-132374	Xenopus tropicalis	67.01	4.00e-173	601.00
IUUC-Xma-133267	Xiphophorus maculatus	60.73	2.00e-157	549.00
IUUC-Yli-134571	Yarrowia lipolytica	24.37	2.00e-12	67.80

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence