iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Ame-011537

Ensembl Protein ID

ENSAMXP00000025230.1

UniProt Accession

W5LR09; W5LR09_ASTMX

Genbank Protein ID

ENSAMXP00000025230

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Genbank Nucleotide ID

APWO01104705

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSAMXG00000024530.1	ENSAMXT00000025246.1	ENSAMXP00000025230.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	3.90e-07	30.7	380	418

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+++C+  w       ++++ CP+Cr
   Q: 380  CIICSELFIE-AVTLNCAHSFCLHCISDW------RRRKDECPICR 418
           ********99.899***************......**********8 PP

Organism

Astyanax mexicanus

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

METVGEDQKR PEEGDHQDDK VWCLKRVGKD SGWLRLTEDT EVTIGRGLNV THQILSVSCP 60
LMISRNHCML KQKEDGQWTV TDNESMNGVW VNGVRISARK PYTLQQDDVV RFGVPLDSNP 120

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Nucleotide Sequence
(Fasta)

CAGAGTCAGG AGAACTGCAG ACTATATAGG GGTCCCTTAA ATAATAGGTT GAACTTTTAT 60
TTTGGAATTG AGCGCGCCAT GAAAAATCGC TCTCGTTGTT GTCGGATCTG TTGTCACATA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000520	Aegilops tauschii	38.10	3.00e-08	52.00
IUUC-Aml-002124	Ailuropoda melanoleuca	41.68	2.00e-72	266.00
IUUC-Atr-002441	Amborella trichopoda	31.73	2.00e-10	59.70
IUUC-Apl-003664	Anas platyrhynchos	74.74	4.00e-37	146.00
IUUC-Aca-005036	Anolis carolinensis	42.32	5.00e-76	278.00
IUUC-Bta-013297	Bos taurus	40.76	4.00e-74	271.00
IUUC-Bol-015906	Brassica oleracea	34.62	1.00e-08	53.10
IUUC-Bra-017421	Brassica rapa	34.62	1.00e-08	53.10
IUUC-Cja-019201	Callithrix jacchus	41.77	2.00e-80	293.00
IUUC-Cfa-021041	Canis familiaris	40.42	3.00e-79	289.00
IUUC-Cre-022788	Chlamydomonas reinhardtii	25.81	4.00e-08	52.40
IUUC-Csa-024133	Chlorocebus sabaeus	42.11	8.00e-84	304.00
IUUC-Cho-024624	Choloepus hoffmanni	33.83	4.00e-35	142.00
IUUC-Dre-027918	Danio rerio	66.52	1.00e-156	546.00
IUUC-Dno-029774	Dasypus novemcinctus	41.37	7.00e-81	294.00
IUUC-Dor-030190	Dipodomys ordii	34.82	2.00e-50	192.00
IUUC-Ete-032301	Echinops telfairi	38.74	4.00e-53	202.00
IUUC-Eca-033607	Equus caballus	40.82	1.00e-64	240.00
IUUC-Eeu-034888	Erinaceus europaeus	50.20	2.00e-54	206.00
IUUC-Fca-036075	Felis catus	41.83	2.00e-82	299.00
IUUC-Fal-037569	Ficedula albicollis	42.76	2.00e-71	263.00
IUUC-Gmo-038588	Gadus morhua	47.02	7.00e-96	344.00
IUUC-Gga-040777	Gallus gallus	42.49	5.00e-80	291.00
IUUC-Gac-042463	Gasterosteus aculeatus	54.00	2.00e-95	343.00
IUUC-Ggo-045269	Gorilla gorilla	42.23	1.00e-85	310.00
IUUC-Hsa-046249	Homo sapiens	42.74	2.00e-81	296.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	42.74	9.00e-84	303.00
IUUC-Lch-049805	Latimeria chalumnae	47.89	1.00e-96	346.00
IUUC-Lpe-050699	Leersia perrieri	32.50	2.00e-09	55.80
IUUC-Loc-052377	Lepisosteus oculatus	53.29	5.00e-120	424.00
IUUC-Mcc-055344	Macaca mulatta	41.81	7.00e-84	304.00
IUUC-Meu-055950	Macropus eugenii	39.36	1.00e-64	240.00
IUUC-Mtr-058038	Medicago truncatula	33.65	3.00e-10	58.90
IUUC-Mla-059052	Melampsora laricipopulina	38.03	2.00e-10	58.90
IUUC-Mga-060049	Meleagris gallopavo	41.24	9.00e-79	287.00
IUUC-Mmr-061431	Microcebus murinus	41.75	5.00e-78	285.00
IUUC-Mdo-062016	Monodelphis domestica	41.50	2.00e-63	238.00
IUUC-Mmu-063235	Mus musculus	40.68	1.00e-86	313.00
IUUC-Mpu-066449	Mustela putorius furo	41.98	4.00e-84	305.00
IUUC-Mlu-067588	Myotis lucifugus	42.28	5.00e-73	268.00
IUUC-Nle-069192	Nomascus leucogenys	42.23	8.00e-86	310.00
IUUC-Opr-070300	Ochotona princeps	34.53	2.00e-62	233.00
IUUC-Ont-071998	Oreochromis niloticus	54.57	1.00e-96	347.00
IUUC-Oan-073610	Ornithorhynchus anatinus	59.52	1.00e-51	196.00
IUUC-Ocu-074603	Oryctolagus cuniculus	42.68	1.00e-79	290.00
IUUC-Ola-086681	Oryzias latipes	48.04	9.00e-103	367.00
IUUC-Oga-088183	Otolemur garnettii	41.89	8.00e-82	297.00
IUUC-Oar-090255	Ovis aries	40.44	5.00e-67	248.00
IUUC-Ptr-091029	Pan troglodytes	42.32	1.00e-77	283.00
IUUC-Pan-092241	Papio anubis	41.81	3.00e-84	305.00
IUUC-Psi-093506	Pelodiscus sinensis	42.86	7.00e-77	281.00
IUUC-Pma-094579	Petromyzon marinus	56.67	6.00e-27	115.00
IUUC-Pfo-097014	Poecilia formosa	53.86	3.00e-99	355.00
IUUC-Pab-098530	Pongo abelii	41.96	3.00e-78	285.00
IUUC-Pca-100939	Procavia capensis	32.37	1.00e-46	180.00
IUUC-Ppe-101294	Prunus persica	34.29	3.00e-10	58.50
IUUC-Pva-103148	Pteropus vampyrus	38.61	4.00e-69	255.00
IUUC-Rno-105338	Rattus norvegicus	42.16	9.00e-91	327.00
IUUC-Sha-107187	Sarcophilus harrisii	64.91	2.00e-51	196.00
IUUC-Sar-113356	Sorex araneus	36.27	3.00e-49	189.00
IUUC-Ssc-116290	Sus scrofa	42.59	3.00e-85	309.00
IUUC-Tru-117910	Takifugu rubripes	50.52	3.00e-99	355.00
IUUC-Tsy-119329	Tarsius syrichta	40.93	4.00e-68	251.00
IUUC-Tni-120521	Tetraodon nigroviridis	50.11	7.00e-96	343.00
IUUC-Ttr-128997	Tursiops truncatus	34.88	2.00e-65	243.00
IUUC-Vpa-130154	Vicugna pacos	38.75	4.00e-75	275.00
IUUC-Xtr-132805	Xenopus tropicalis	42.53	6.00e-86	311.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence