iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Ath-007600

UUCD1 version

UUC-ArT-01655

Ensembl Protein ID

AT2G32410.1

UniProt Accession

Q9ZV69; AXL1_ARATH

Genbank Protein ID

AAC69937.1; AEC08680.1; AT2G32410.1

Protein Name

NEDD8-activating enzyme E1 regulatory subunit AXL; Protein AXR1-LIKE; Protein AXR1-LIKE 1

Genbank Nucleotide ID

AC005700; CP002685; BX819419

Gene Name

AXL1; AXL; At2g32410

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
AT2G32410	AT2G32410.2	AT2G32410.2
AT2G32410	AT2G32410.1	AT2G32410.1
AT2G32410	AT2G32410.3	AT2G32410.3

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
DNA & RNA Element
TRANSFAC
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	3.50e-118	396.3	8	514

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesit 98
           YDRQ+r+wGe +q +l++a+++l+++g++G+E lKnLv +G+gsit+vD+++ve+ +l+++F+++a++vg+s+A++++ +l+eln++v++ ++ee+++
   Q: 8    YDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPD 105
           ************************************************************************************************** PP
   S: 99   eleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn.........aa 183
           +l  +++ Ff++f++V++++  ++++ k++r+c++a+v l+ ++++Gl G+vr+++k++t+++ ++d   d+++++ P+++lk++++         aa
   Q: 106  TLISTDPsFFSQFTLVIATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDhslDDLRLNSPWPELKSYVEsidlnveepAA 203
           *************************************************************************************88888889999** PP
   S: 184  ehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv...... 274
           +++i+++v++ k++ee+a+ ++++ ++++eek e+kd+vks+++s+deen++ea  +a+k fa+t i++ i+++ ++ +c++v +++s+fwv      
   Q: 204  HKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKDLVKSKMVSADEENYKEALLAAFKVFAPTGISQeIQDINHD-SCAEVGSNSSDFWVmvaalk 300
           *********************************************************************99999998.******************** PP
   S: 275  .....................................................................sfcsnaealq...........vpekvekd 292
                                                                                sfc+na++l+             +++++ 
   Q: 301  efisnegggevplegsmpdmisstehyinlqkiyhskaeadflsmeqrvksilvkvgqdpssiskptikSFCKNARKLKvcryrtiedefKSPSTTEL 398
           *******************************************************************************6666665555544888888 PP
   S: 293  eevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattnavvgGlaaqEv 384
            ++++++++     +y+llra++rf+ ++++ pg+++   d+d a +l+t a++l  +++g+ + +l+++l  e++++++++i++++a +gG+ +qEv
   Q: 399  HKYLADENYsgaigFYILLRAVDRFAGTYKKFPGQFDGSTDED-ASQLKTIALSLL-SEMGCDGyELQEELYNEMCRFGAAEIHVVAALIGGITSQEV 494
           888888888**********************************.******999998.***************************************** PP
   S: 385  iKvvsgkfeplknfflfdae 404
           iK+++++f+p + +f+f+++
   Q: 495  IKLITKQFVPKRGTFIFNGI 514
           *****************986 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Regulatory subunit of the dimeric ECR1-AXL1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1 (Probable). May function redundantly with AXR1 in the RUB conjugating pathway (PubMed:17655650). Seems not to be functionally equivalent to AXR1 in vivo (PubMed:21311953).

Protein Sequence
(Fasta)

MAEPKTKYDR QLRIWGELGQ SALETASICL LNCGPTGSEA LKNLVIGGIG SITIVDGSKV 60
EIGDLGNNFM VDAKSVGQSR AKTVCGFLQE LNDSVKANFV EENPDTLIST DPSFFSQFTL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CTCTCTCTCT CTCTCTCTCT CTCTCTCTGC GTCTTTCTCT TTCTCTCTGT CTGAAGTTGA 60
GAACACGAAC CACTGAAAAA CCCGCGAAAG ACTCGAGCAC GAACCAACAA TGGCGGAACC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0005634--C:nucleus
GO:0005777--C:peroxisome
GO:0019781--F:NEDD8 activating enzyme activity
GO:0000166--F:nucleotide binding
GO:0010252--P:auxin homeostasis
GO:0009791--P:post-embryonic development
GO:0045116--P:protein neddylation

KEGG

ath:AT2G32410

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002333	Ailuropoda melanoleuca	40.60	4.00e-126	444.00
IUUC-Apl-003446	Anas platyrhynchos	39.62	2.00e-117	415.00
IUUC-Aca-004450	Anolis carolinensis	39.69	8.00e-123	433.00
IUUC-Aly-006187	Arabidopsis lyrata	91.59	0.00e+00	1000.00
IUUC-Ago-007900	Ashbya gossypii	25.88	4.00e-37	148.00
IUUC-Acl-008333	Aspergillus clavatus	34.06	1.00e-71	263.00
IUUC-Afl-008529	Aspergillus flavus	33.51	4.00e-77	281.00
IUUC-Afu-009071	Aspergillus fumigatus	33.58	7.00e-72	264.00
IUUC-Ang-009669	Aspergillus niger	32.67	1.00e-75	276.00
IUUC-Aor-009914	Aspergillus oryzae	33.39	4.00e-76	278.00
IUUC-Ate-010450	Aspergillus terreus	32.24	9.00e-72	263.00
IUUC-Ame-011183	Astyanax mexicanus	40.64	4.00e-125	440.00
IUUC-Bta-012583	Bos taurus	40.93	2.00e-128	451.00
IUUC-Bra-017506	Brassica rapa	81.90	0.00e+00	894.00
IUUC-Cja-020007	Callithrix jacchus	40.33	1.00e-125	442.00
IUUC-Cfa-020324	Canis familiaris	40.93	1.00e-128	452.00
IUUC-Cpo-022248	Cavia porcellus	36.99	3.00e-71	261.00
IUUC-Csa-023149	Chlorocebus sabaeus	37.50	1.00e-98	353.00
IUUC-Cho-024537	Choloepus hoffmanni	33.98	1.00e-85	309.00
IUUC-Cin-025763	Ciona intestinalis	39.10	7.00e-109	387.00
IUUC-Csv-026113	Ciona savignyi	39.19	2.00e-107	382.00
IUUC-Dre-028140	Danio rerio	41.76	2.00e-128	451.00
IUUC-Dno-030033	Dasypus novemcinctus	40.83	1.00e-110	392.00
IUUC-Dor-030513	Dipodomys ordii	27.75	3.00e-51	195.00
IUUC-Dse-031536	Dothistroma septosporum	33.27	5.00e-70	258.00
IUUC-Ete-032420	Echinops telfairi	36.75	6.00e-109	387.00
IUUC-Eca-033992	Equus caballus	38.51	2.00e-98	352.00
IUUC-Eeu-034713	Erinaceus europaeus	31.37	5.00e-36	145.00
IUUC-Fca-036548	Felis catus	40.68	2.00e-126	445.00
IUUC-Fal-037600	Ficedula albicollis	39.69	2.00e-116	412.00
IUUC-Gga-041129	Gallus gallus	39.55	4.00e-120	424.00
IUUC-Gac-042558	Gasterosteus aculeatus	40.30	1.00e-123	436.00
IUUC-Ggo-045611	Gorilla gorilla	40.80	7.00e-125	440.00
IUUC-Hsa-046563	Homo sapiens	41.03	5.00e-126	444.00
IUUC-Itr-048066	Ictidomys tridecemlineatus	40.75	1.00e-126	446.00
IUUC-Lch-050038	Latimeria chalumnae	40.42	2.00e-120	425.00
IUUC-Loc-051917	Lepisosteus oculatus	37.11	3.00e-108	384.00
IUUC-Laf-053690	Loxodonta africana	40.56	2.00e-126	445.00
IUUC-Mcc-055117	Macaca mulatta	40.07	7.00e-123	433.00
IUUC-Meu-056761	Macropus eugenii	32.84	2.00e-89	322.00
IUUC-Mla-059183	Melampsora laricipopulina	35.73	6.00e-88	317.00
IUUC-Mga-060144	Meleagris gallopavo	39.16	3.00e-116	411.00
IUUC-Mmr-061325	Microcebus murinus	39.03	6.00e-122	430.00
IUUC-Mdo-062532	Monodelphis domestica	41.48	3.00e-127	447.00
IUUC-Mmu-063956	Mus musculus	40.75	9.00e-128	449.00
IUUC-Mpu-066716	Mustela putorius furo	39.96	1.00e-117	416.00
IUUC-Mlu-067459	Myotis lucifugus	40.57	2.00e-125	442.00
IUUC-Ncr-068910	Neurospora crassa	31.65	6.00e-66	244.00
IUUC-Nle-070034	Nomascus leucogenys	39.58	3.00e-120	424.00
IUUC-Opr-070552	Ochotona princeps	37.85	6.00e-99	353.00
IUUC-Oan-073389	Ornithorhynchus anatinus	37.50	3.00e-91	328.00
IUUC-Ocu-074911	Oryctolagus cuniculus	40.56	3.00e-127	448.00
IUUC-Oga-088810	Otolemur garnettii	40.75	3.00e-127	447.00
IUUC-Oar-089196	Ovis aries	40.71	8.00e-127	446.00
IUUC-Ptr-090617	Pan troglodytes	41.03	3.00e-126	444.00
IUUC-Pan-092761	Papio anubis	40.75	2.00e-127	448.00
IUUC-Psi-093191	Pelodiscus sinensis	38.61	2.00e-119	422.00
IUUC-Pma-094380	Petromyzon marinus	40.73	1.00e-112	399.00
IUUC-Pfo-096467	Poecilia formosa	40.61	6.00e-122	430.00
IUUC-Pab-098255	Pongo abelii	40.67	1.00e-107	382.00
IUUC-Pca-100807	Procavia capensis	48.13	1.00e-52	199.00
IUUC-Pgr-103401	Puccinia graminis	30.15	2.00e-75	275.00
IUUC-Ptt-103889	Puccinia triticina	28.67	1.00e-63	237.00
IUUC-Rno-105810	Rattus norvegicus	40.65	2.00e-125	441.00
IUUC-Sce-106397	Saccharomyces cerevisiae	26.69	5.00e-33	134.00
IUUC-Sha-106770	Sarcophilus harrisii	46.13	2.00e-71	261.00
IUUC-Sja-107926	Schizosaccharomyces japonicus	33.33	2.00e-78	285.00
IUUC-Spo-108276	Schizosaccharomyces pombe	31.54	9.00e-81	293.00
IUUC-Smo-109296	Selaginella moellendorffii	54.71	2.00e-165	574.00
IUUC-Sar-113083	Sorex araneus	32.50	3.00e-85	308.00
IUUC-Tgu-116772	Taeniopygia guttata	40.68	3.00e-117	414.00
IUUC-Tsy-119111	Tarsius syrichta	43.38	1.00e-63	236.00
IUUC-Tni-120231	Tetraodon nigroviridis	37.90	5.00e-109	387.00
IUUC-Tre-122103	Trichoderma reesei	32.52	1.00e-68	253.00
IUUC-Tme-127097	Tuber melanosporum	35.53	3.00e-81	295.00
IUUC-Ttr-128465	Tursiops truncatus	38.10	9.00e-113	399.00
IUUC-Uma-129645	Ustilago maydis	31.69	3.00e-74	272.00
IUUC-Vpa-130529	Vicugna pacos	39.22	3.00e-117	414.00
IUUC-Xtr-132002	Xenopus tropicalis	41.98	9.00e-128	449.00
IUUC-Xma-133930	Xiphophorus maculatus	40.92	7.00e-125	440.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence