iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-064168

UUCD1 version

UUC-MuM-01099

Ensembl Protein ID

ENSMUSP00000020550.5

UniProt Accession

Q8CFI2; Q505K8; UB2R1_MOUSE

Genbank Protein ID

AAH39160.1; AAH94502.1

Protein Name

Ubiquitin-conjugating enzyme E2 R1; (E3-independent) E2 ubiquitin-conjugating enzyme R1; E2 ubiquitin-conjugating enzyme R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1

Genbank Nucleotide ID

BC039160; BC094502

Gene Name

Cdc34; Ubch3; Ube2r1

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000020307.14	ENSMUST00000020550.11	ENSMUSP00000020550.5

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
microRNA	TRANSFAC	RepTar	RAID2
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	8.70e-48	162.7	15	153

Active Site

Position(s)	Description	Evidence
93	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 4    kelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilk..... 87
            elk l+++p+eg+++++vde dl++wev+i+Gp++t+Yegg+Fk++++fp dYP++PP ++fltk++hPn+ye+G+vC+sil+     
   Q: 15   LELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHppvdd 103
           599********************************************************************************999999 PP
   S: 88   ......eeekWspalsvesvllsiqsllaepnpesplneeaaellkknre 131
                 ++e+W+p+++v+++lls++sll+epn+ sp+n++a+ +++k++e
   Q: 104  pqsgelPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKE 153
           999*9******************************************998 PP

Organism

Mus musculus

Functional Description
(View)

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin.

Protein Sequence
(Fasta)

MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA 60
RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CGCGGGCCGG GCCGGGCCGA AGCGAGCGCC GGGCGGGCAG CGGCGCCAGA GCGAGTGGAG 60
CCTTCGGGGT CCCCGGGTGG CGGCGGCGGC GGCGGTGCAG AGGAGGAAGG CGGCGGCTCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0016607--C:nuclear speck
GO:0005524--F:ATP binding
GO:0061631--F:ubiquitin conjugating enzyme activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0007049--P:cell cycle
GO:0035458--P:cellular response to interferon-beta
GO:0043951--P:negative regulation of cAMP-mediated signaling
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0070936--P:protein K48-linked ubiquitination
GO:0006513--P:protein monoubiquitination
GO:0000209--P:protein polyubiquitination
GO:0016567--P:protein ubiquitination

KEGG

mmu:216150

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001600	Ailuropoda melanoleuca	99.11	2.00e-129	454.00
IUUC-Ame-011425	Astyanax mexicanus	89.55	1.00e-108	385.00
IUUC-Bta-012516	Bos taurus	99.51	2.00e-120	424.00
IUUC-Cja-018852	Callithrix jacchus	90.00	2.00e-103	368.00
IUUC-Cfa-020203	Canis familiaris	100.00	1.00e-120	425.00
IUUC-Cpo-022139	Cavia porcellus	92.07	3.00e-121	427.00
IUUC-Csa-023371	Chlorocebus sabaeus	95.98	2.00e-121	427.00
IUUC-Dre-028367	Danio rerio	89.05	3.00e-108	384.00
IUUC-Dor-030875	Dipodomys ordii	96.00	6.00e-122	429.00
IUUC-Fca-035832	Felis catus	98.19	1.00e-93	334.00
IUUC-Gmo-039414	Gadus morhua	90.05	1.00e-109	388.00
IUUC-Gga-040694	Gallus gallus	93.64	7.00e-122	429.00
IUUC-Gac-042485	Gasterosteus aculeatus	88.56	5.00e-109	386.00
IUUC-Ggo-044806	Gorilla gorilla	95.98	2.00e-121	428.00
IUUC-Hsa-046245	Homo sapiens	95.98	2.00e-121	427.00
IUUC-Loc-052932	Lepisosteus oculatus	91.54	4.00e-111	393.00
IUUC-Mcc-054665	Macaca mulatta	92.41	3.00e-114	404.00
IUUC-Mga-060075	Meleagris gallopavo	93.22	2.00e-121	427.00
IUUC-Mmr-061165	Microcebus murinus	99.10	9.00e-123	432.00
IUUC-Mdo-061825	Monodelphis domestica	99.51	4.00e-120	423.00
IUUC-Mpu-066009	Mustela putorius furo	99.11	5.00e-129	452.00
IUUC-Opr-070826	Ochotona princeps	90.23	2.00e-108	385.00
IUUC-Ont-071745	Oreochromis niloticus	90.15	1.00e-110	392.00
IUUC-Oan-073287	Ornithorhynchus anatinus	90.25	2.00e-116	411.00
IUUC-Ocu-074902	Oryctolagus cuniculus	95.63	2.00e-116	411.00
IUUC-Oga-088845	Otolemur garnettii	97.76	1.00e-127	448.00
IUUC-Ptr-090916	Pan troglodytes	98.65	6.00e-119	419.00
IUUC-Pan-092577	Papio anubis	95.98	2.00e-121	427.00
IUUC-Psi-093039	Pelodiscus sinensis	91.01	5.00e-87	313.00
IUUC-Pfo-096435	Poecilia formosa	89.55	9.00e-110	389.00
IUUC-Pab-098200	Pongo abelii	95.61	3.00e-110	390.00
IUUC-Pca-100169	Procavia capensis	86.43	5.00e-66	243.00
IUUC-Pva-102875	Pteropus vampyrus	100.00	7.00e-85	305.00
IUUC-Rno-106079	Rattus norvegicus	100.00	2.00e-121	427.00
IUUC-Ssc-115699	Sus scrofa	89.25	7.00e-88	315.00
IUUC-Tgu-117466	Taeniopygia guttata	91.53	1.00e-119	421.00
IUUC-Tni-120864	Tetraodon nigroviridis	86.07	2.00e-105	374.00
IUUC-Xtr-132285	Xenopus tropicalis	88.61	8.00e-116	409.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence