iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Mga-060049

UUCD1 version

UUC-MeG-00454

Ensembl Protein ID

ENSMGAP00000011185.2

UniProt Accession

G1NE57; G1NE57_MELGA

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Gene Name

RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMGAG00000010761.2	ENSMGAT00000012055.2	ENSMGAP00000011185.2

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	5.50e-07	29.8	402	440

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C iC e+f +  ++l+C H f+  C+  w      ++++  CP+Cr
   Q: 402  CTICSEHFIE-AVTLNCAHSFCSYCIDEW------TKRKVECPICR 440
           ********99.899***************......**********8 PP

Organism

Meleagris gallopavo

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MTECSIGITV QLRYCKKRPA RSPGEDLHLP PHLEVTVGRG LDLTYQLVSK TCPLMISRKH 60
CVFQQNAEGQ WTVKDNKSLN GVWLNKQRLD PSKTYPLAEG DRIQLGVPLE NKETAEYEYE 120

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Nucleotide Sequence
(Fasta)

ATGACAGAGT GCAGCATTGG TATCACAGTA CAGCTACGAT ACTGTAAGAA AAGGCCTGCA 60
CGTTCCCCAG GTGAGGACTT GCACTTGCCA CCTCACCTTG AAGTAATCCC CAAAGGACCA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0000151--C:ubiquitin ligase complex
GO:0003682--F:chromatin binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0006302--P:double-strand break repair
GO:0070535--P:histone H2A K63-linked ubiquitination
GO:0034244--P:negative regulation of transcription elongation from RNA polymerase II promoter
GO:0045739--P:positive regulation of DNA repair
GO:0051865--P:protein autoubiquitination
GO:0070936--P:protein K48-linked ubiquitination
GO:0010212--P:response to ionizing radiation
GO:0006511--P:ubiquitin-dependent protein catabolic process

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	57.23	4.00e-102	364.00
IUUC-Apl-003664	Anas platyrhynchos	97.73	3.00e-48	183.00
IUUC-Aca-005036	Anolis carolinensis	64.51	9.00e-131	459.00
IUUC-Ago-007705	Ashbya gossypii	45.61	9.00e-09	54.30
IUUC-Ame-011537	Astyanax mexicanus	42.16	9.00e-83	300.00
IUUC-Bta-013297	Bos taurus	60.31	8.00e-108	383.00
IUUC-Cja-019201	Callithrix jacchus	58.89	5.00e-114	404.00
IUUC-Cfa-021041	Canis familiaris	60.08	5.00e-121	427.00
IUUC-Csa-024133	Chlorocebus sabaeus	57.32	3.00e-119	421.00
IUUC-Cho-024624	Choloepus hoffmanni	52.10	2.00e-71	262.00
IUUC-Cgl-026486	Colletotrichum gloeosporioides	41.27	1.00e-08	54.70
IUUC-Dre-027918	Danio rerio	41.93	3.00e-77	282.00
IUUC-Dno-029774	Dasypus novemcinctus	60.72	2.00e-123	435.00
IUUC-Dor-030190	Dipodomys ordii	52.89	3.00e-87	315.00
IUUC-Dse-031157	Dothistroma septosporum	36.59	3.00e-08	53.10
IUUC-Ete-032301	Echinops telfairi	53.81	4.00e-90	324.00
IUUC-Eca-033607	Equus caballus	60.31	5.00e-108	384.00
IUUC-Eeu-034888	Erinaceus europaeus	59.79	6.00e-76	277.00
IUUC-Fca-036075	Felis catus	60.00	8.00e-118	416.00
IUUC-Fal-037569	Ficedula albicollis	79.76	1.00e-170	592.00
IUUC-Fox-037681	Fusarium oxysporum	41.27	8.00e-09	55.10
IUUC-Fso-038129	Fusarium solani	33.05	4.00e-09	55.80
IUUC-Gmo-038588	Gadus morhua	39.61	8.00e-70	257.00
IUUC-Ggr-039871	Gaeumannomyces graminis	41.27	8.00e-09	55.50
IUUC-Gga-040777	Gallus gallus	94.62	0.00e+00	711.00
IUUC-Gac-042463	Gasterosteus aculeatus	42.62	2.00e-59	223.00
IUUC-Ggo-045269	Gorilla gorilla	57.84	6.00e-119	420.00
IUUC-Hsa-046249	Homo sapiens	57.96	5.00e-117	414.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	59.65	1.00e-118	419.00
IUUC-Kpa-049298	Komagataella pastoris	42.31	5.00e-07	48.10
IUUC-Lch-049805	Latimeria chalumnae	52.71	4.00e-108	384.00
IUUC-Lpe-050699	Leersia perrieri	36.00	2.00e-08	52.40
IUUC-Loc-052377	Lepisosteus oculatus	44.82	1.00e-81	296.00
IUUC-Laf-053820	Loxodonta africana	32.56	2.00e-08	54.70
IUUC-Mcc-055344	Macaca mulatta	57.29	6.00e-118	417.00
IUUC-Meu-055950	Macropus eugenii	60.59	4.00e-112	397.00
IUUC-Mor-057012	Magnaporthe oryzae	35.96	4.00e-10	59.70
IUUC-Mla-059052	Melampsora laricipopulina	36.71	3.00e-11	61.20
IUUC-Mmr-061431	Microcebus murinus	57.87	1.00e-110	392.00
IUUC-Mdo-062016	Monodelphis domestica	67.27	3.00e-119	423.00
IUUC-Mmu-063235	Mus musculus	55.03	9.00e-117	413.00
IUUC-Mpu-066449	Mustela putorius furo	59.36	5.00e-122	430.00
IUUC-Mlu-067588	Myotis lucifugus	60.09	1.00e-107	382.00
IUUC-Ncr-068692	Neurospora crassa	41.27	1.00e-08	54.70
IUUC-Nle-069192	Nomascus leucogenys	58.05	5.00e-119	420.00
IUUC-Opr-070300	Ochotona princeps	49.22	4.00e-88	318.00
IUUC-Ont-071998	Oreochromis niloticus	43.25	1.00e-63	236.00
IUUC-Oan-073610	Ornithorhynchus anatinus	64.04	4.00e-64	236.00
IUUC-Ocu-074603	Oryctolagus cuniculus	60.98	7.00e-119	420.00
IUUC-Ogu-078576	Oryza glumaepatula	36.00	3.00e-08	51.60
IUUC-Oni-082120	Oryza nivara	36.00	3.00e-08	51.60
IUUC-Oru-084679	Oryza rufipogon	36.00	3.00e-08	51.20
IUUC-Ola-086681	Oryzias latipes	40.79	9.00e-71	260.00
IUUC-Oga-088183	Otolemur garnettii	58.39	7.00e-117	413.00
IUUC-Oar-090255	Ovis aries	59.87	6.00e-109	387.00
IUUC-Ptr-091029	Pan troglodytes	59.20	3.00e-120	424.00
IUUC-Pan-092241	Papio anubis	57.20	1.00e-117	416.00
IUUC-Psi-093506	Pelodiscus sinensis	74.73	6.00e-157	546.00
IUUC-Pma-094579	Petromyzon marinus	57.78	3.00e-26	112.00
IUUC-Pfo-097014	Poecilia formosa	44.78	5.00e-64	238.00
IUUC-Pab-098530	Pongo abelii	58.90	7.00e-118	416.00
IUUC-Pca-100939	Procavia capensis	48.52	5.00e-73	268.00
IUUC-Pva-103148	Pteropus vampyrus	53.86	1.00e-106	379.00
IUUC-Rno-105338	Rattus norvegicus	55.58	4.00e-117	414.00
IUUC-Sce-106402	Saccharomyces cerevisiae	45.61	4.00e-09	55.80
IUUC-Sha-107187	Sarcophilus harrisii	63.88	2.00e-97	348.00
IUUC-Sja-107948	Schizosaccharomyces japonicus	25.56	1.00e-09	57.40
IUUC-Sar-113356	Sorex araneus	49.88	2.00e-84	305.00
IUUC-Sre-115087	Sporisorium reilianum	40.43	7.00e-06	45.80
IUUC-Ssc-116290	Sus scrofa	59.36	3.00e-131	461.00
IUUC-Tru-117910	Takifugu rubripes	44.00	3.00e-62	231.00
IUUC-Tsy-119329	Tarsius syrichta	55.56	4.00e-100	357.00
IUUC-Tni-120521	Tetraodon nigroviridis	42.83	3.00e-65	242.00
IUUC-Tre-121954	Trichoderma reesei	41.27	7.00e-09	55.10
IUUC-Tvi-122295	Trichoderma virens	41.27	1.00e-08	54.30
IUUC-Tme-126881	Tuber melanosporum	42.86	3.00e-08	53.10
IUUC-Ttr-128997	Tursiops truncatus	54.79	8.00e-111	393.00
IUUC-Vda-130024	Verticillium dahliae	41.27	5.00e-09	55.80
IUUC-Vpa-130154	Vicugna pacos	58.17	4.00e-120	424.00
IUUC-Xtr-132805	Xenopus tropicalis	53.11	2.00e-105	375.00
IUUC-Xma-133620	Xiphophorus maculatus	44.66	8.00e-63	234.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence