iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Nfi-068551

Ensembl Protein ID

CADNFIAP00007777

UniProt Accession

A1DF15; ATG3_NEOFI

Genbank Protein ID

EAW17972.1

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme ATG3

Genbank Nucleotide ID

DS027696

Gene Name

NFIA_079130; atg3

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
CADNFIAG00007944	CADNFIAT00007944	CADNFIAP00007777

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	30.79	9.00e-37	151.00	IUUC-Ath-006802
E2/Autophagy_C	36.30	9.00e-35	145.00	IUUC-Hsa-046449
E2/Autophagy_C	38.31	9.00e-56	212.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
245	Glycyl thioester intermediate	{ECO:0000250}

Organism

Neosartorya fischeri

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNILHSTLST WRDRLAPVSR TSTFRTTGQI TPEEFVLAGD YLVYKFPTWA WADASSPAKR 60
VSYLPPGKQF LVTRGVPCHR RLNENFAGDA GHEDEIVRDM LSGADADDGD GWLRTGGGRD 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGAACATCC TCCACTCCAC CTTATCAACC TGGCGGGATC GCCTGGCCCC CGTCTCCCGC 60
ACCTCCACCT TCCGCACCAC CGGCCAAATC ACCCCAGAGG AGTTCGTCCT GGCAGGCGAC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

KEGG

nfi:NFIA_079130

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	35.55	2.00e-32	130.00
IUUC-Aml-002081	Ailuropoda melanoleuca	36.00	2.00e-36	144.00
IUUC-Atr-002712	Amborella trichopoda	33.33	4.00e-31	126.00
IUUC-Apl-003956	Anas platyrhynchos	25.98	8.00e-20	89.00
IUUC-Aca-004578	Anolis carolinensis	38.15	7.00e-36	142.00
IUUC-Aly-005823	Arabidopsis lyrata	32.11	5.00e-41	159.00
IUUC-Ath-006802	Arabidopsis thaliana	31.84	4.00e-40	156.00
IUUC-Ago-007855	Ashbya gossypii	36.62	1.00e-53	201.00
IUUC-Acl-008147	Aspergillus clavatus	83.38	1.00e-157	547.00
IUUC-Afl-008483	Aspergillus flavus	82.63	4.00e-151	525.00
IUUC-Afu-008818	Aspergillus fumigatus	98.02	0.00e+00	654.00
IUUC-Ani-009481	Aspergillus nidulans	76.96	1.00e-140	490.00
IUUC-Ang-009800	Aspergillus niger	72.83	8.00e-139	484.00
IUUC-Aor-010150	Aspergillus oryzae	82.63	4.00e-151	525.00
IUUC-Ate-010545	Aspergillus terreus	79.67	9.00e-84	301.00
IUUC-Ame-011793	Astyanax mexicanus	37.59	8.00e-35	139.00
IUUC-Bgr-012167	Blumeria graminis	62.50	3.00e-107	379.00
IUUC-Bta-012695	Bos taurus	31.04	2.00e-38	150.00
IUUC-Bci-013972	Botrytis cinerea	66.58	1.00e-100	357.00
IUUC-Bdi-014756	Brachypodium distachyon	32.96	2.00e-38	150.00
IUUC-Bol-015091	Brassica oleracea	31.71	8.00e-39	152.00
IUUC-Bra-017220	Brassica rapa	33.15	1.00e-39	154.00
IUUC-Cel-018143	Caenorhabditis elegans	34.53	2.00e-36	144.00
IUUC-Cja-018991	Callithrix jacchus	31.08	2.00e-39	154.00
IUUC-Cfa-020070	Canis familiaris	36.30	2.00e-36	144.00
IUUC-Cpo-021860	Cavia porcellus	31.32	2.00e-38	150.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	30.16	2.00e-36	144.00
IUUC-Csa-023881	Chlorocebus sabaeus	31.87	5.00e-39	152.00
IUUC-Cho-024852	Choloepus hoffmanni	32.60	1.00e-19	88.60
IUUC-Cin-025787	Ciona intestinalis	34.94	3.00e-35	140.00
IUUC-Csv-026369	Ciona savignyi	37.08	9.00e-37	145.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	61.13	3.00e-98	349.00
IUUC-Cne-026905	Cryptococcus neoformans	36.17	3.00e-23	100.00
IUUC-Dre-028811	Danio rerio	37.36	4.00e-35	139.00
IUUC-Dno-030081	Dasypus novemcinctus	32.24	4.00e-37	146.00
IUUC-Dor-030183	Dipodomys ordii	45.19	4.00e-20	90.10
IUUC-Dse-031539	Dothistroma septosporum	52.33	4.00e-66	243.00
IUUC-Dme-031805	Drosophila melanogaster	50.00	3.00e-19	87.00
IUUC-Ete-033171	Echinops telfairi	44.23	8.00e-20	89.00
IUUC-Eca-034083	Equus caballus	31.04	3.00e-38	150.00
IUUC-Eeu-034804	Erinaceus europaeus	30.77	4.00e-36	143.00
IUUC-Fca-036289	Felis catus	31.17	3.00e-38	150.00
IUUC-Fal-037060	Ficedula albicollis	46.15	4.00e-19	87.00
IUUC-Fox-038051	Fusarium oxysporum	63.97	8.00e-110	388.00
IUUC-Fso-038124	Fusarium solani	64.71	5.00e-98	348.00
IUUC-Gmo-039538	Gadus morhua	39.25	3.00e-38	150.00
IUUC-Ggr-039880	Gaeumannomyces graminis	59.52	1.00e-108	384.00
IUUC-Gga-041108	Gallus gallus	36.90	8.00e-36	142.00
IUUC-Gac-041558	Gasterosteus aculeatus	35.69	6.00e-36	142.00
IUUC-Gma-043969	Glycine max	29.63	2.00e-38	150.00
IUUC-Ggo-045071	Gorilla gorilla	32.14	1.00e-38	151.00
IUUC-Hsa-046449	Homo sapiens	32.14	1.00e-38	151.00
IUUC-Hvu-047451	Hordeum vulgare	35.94	6.00e-34	135.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	31.04	5.00e-38	149.00
IUUC-Kpa-049096	Komagataella pastoris	43.10	2.00e-35	140.00
IUUC-Lch-049726	Latimeria chalumnae	35.82	1.00e-36	145.00
IUUC-Lpe-050734	Leersia perrieri	38.43	7.00e-41	159.00
IUUC-Loc-051983	Lepisosteus oculatus	36.53	6.00e-35	139.00
IUUC-Lma-053038	Leptosphaeria maculans	62.08	1.00e-113	400.00
IUUC-Laf-054510	Loxodonta africana	35.40	6.00e-36	142.00
IUUC-Mcc-054792	Macaca mulatta	31.87	5.00e-39	152.00
IUUC-Meu-055965	Macropus eugenii	51.90	3.00e-18	84.00
IUUC-Mor-057292	Magnaporthe oryzae	61.22	5.00e-105	372.00
IUUC-Mtr-058282	Medicago truncatula	30.43	2.00e-39	154.00
IUUC-Mla-059144	Melampsora laricipopulina	40.78	1.00e-27	115.00
IUUC-Mga-059509	Meleagris gallopavo	41.54	2.00e-19	87.80
IUUC-Mvi-060281	Microbotryum violaceum	40.56	5.00e-27	113.00
IUUC-Mmr-061788	Microcebus murinus	31.04	1.00e-38	151.00
IUUC-Mdo-062922	Monodelphis domestica	30.77	3.00e-38	150.00
IUUC-Mmu-064249	Mus musculus	35.19	1.00e-35	141.00
IUUC-Mac-064474	Musa acuminata	31.59	5.00e-39	152.00
IUUC-Mpu-065717	Mustela putorius furo	45.19	5.00e-20	89.70
IUUC-Mlu-067151	Myotis lucifugus	30.42	9.00e-33	132.00
IUUC-Ncr-068846	Neurospora crassa	62.67	4.00e-107	379.00
IUUC-Nle-069790	Nomascus leucogenys	32.14	1.00e-38	151.00
IUUC-Opr-070828	Ochotona princeps	23.36	8.70e-02	28.50
IUUC-Ont-072517	Oreochromis niloticus	30.38	4.00e-36	143.00
IUUC-Oan-073285	Ornithorhynchus anatinus	51.90	5.00e-18	82.80
IUUC-Ocu-074695	Oryctolagus cuniculus	31.59	4.00e-38	150.00
IUUC-Oba-075515	Oryza barthii	39.23	5.00e-40	156.00
IUUC-Obr-076787	Oryza brachyantha	35.21	2.00e-42	164.00
IUUC-Ogl-077440	Oryza glaberrima	33.24	1.00e-42	165.00
IUUC-Ogu-078294	Oryza glumaepatula	34.08	3.00e-43	167.00
IUUC-Oin-079452	Oryza indica	34.18	4.00e-43	166.00
IUUC-Olo-080450	Oryza longistaminata	34.75	7.00e-42	162.00
IUUC-Ome-081514	Oryza meridionalis	34.65	7.00e-43	165.00
IUUC-Oni-082677	Oryza nivara	34.18	4.00e-43	166.00
IUUC-Opu-083925	Oryza punctata	34.37	2.00e-42	164.00
IUUC-Oru-084828	Oryza rufipogon	34.38	7.00e-41	159.00
IUUC-Osa-085937	Oryza sativa	39.00	6.00e-40	155.00
IUUC-Ola-086559	Oryzias latipes	33.63	1.00e-24	104.00
IUUC-Olu-087766	Ostreococcus lucimarinus	32.23	8.00e-35	139.00
IUUC-Oga-087983	Otolemur garnettii	34.69	6.00e-35	139.00
IUUC-Oar-089281	Ovis aries	31.32	2.00e-38	150.00
IUUC-Ptr-090983	Pan troglodytes	32.14	1.00e-38	151.00
IUUC-Pan-092457	Papio anubis	31.87	4.00e-39	153.00
IUUC-Psi-094046	Pelodiscus sinensis	36.92	2.00e-33	134.00
IUUC-Pno-094925	Phaeosphaeria nodorum	61.97	1.00e-110	390.00
IUUC-Ppa-095487	Physcomitrella patens	37.13	2.00e-37	147.00
IUUC-Pfo-096968	Poecilia formosa	32.61	2.00e-20	91.30
IUUC-Pab-098621	Pongo abelii	32.14	2.00e-37	147.00
IUUC-Pop-100007	Populus trichocarpa	30.91	6.00e-40	155.00
IUUC-Pca-100590	Procavia capensis	32.60	1.00e-19	88.60
IUUC-Ppe-101657	Prunus persica	31.15	6.00e-39	152.00
IUUC-Pva-102446	Pteropus vampyrus	35.56	1.00e-36	145.00
IUUC-Pgr-103604	Puccinia graminis	49.43	8.00e-22	96.30
IUUC-Ptt-103941	Puccinia triticina	47.83	8.00e-22	96.30
IUUC-Pte-104252	Pyrenophora teres	61.52	1.00e-113	400.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	60.96	1.00e-112	397.00
IUUC-Rno-106025	Rattus norvegicus	35.56	2.00e-36	144.00
IUUC-Sce-106172	Saccharomyces cerevisiae	37.25	4.00e-52	196.00
IUUC-Sha-106858	Sarcophilus harrisii	45.19	4.00e-20	90.10
IUUC-Sja-107905	Schizosaccharomyces japonicus	33.33	4.00e-19	86.30
IUUC-Spo-108185	Schizosaccharomyces pombe	52.56	1.00e-18	85.10
IUUC-Smo-108883	Selaginella moellendorffii	30.11	5.00e-34	136.00
IUUC-Sit-110512	Setaria italica	33.72	4.00e-36	143.00
IUUC-Sly-111238	Solanum lycopersicum	31.47	7.00e-41	159.00
IUUC-Stu-112441	Solanum tuberosum	32.00	2.00e-40	157.00
IUUC-Sar-113039	Sorex araneus	32.61	5.00e-20	89.70
IUUC-Sbi-113776	Sorghum bicolor	36.50	1.00e-33	134.00
IUUC-Sre-115074	Sporisorium reilianum	51.19	5.00e-20	90.50
IUUC-Ssc-115262	Sus scrofa	31.04	5.00e-38	149.00
IUUC-Tgu-116790	Taeniopygia guttata	32.60	1.00e-19	88.60
IUUC-Tru-118160	Takifugu rubripes	31.79	9.00e-37	145.00
IUUC-Tsy-119145	Tarsius syrichta	26.70	3.00e-13	67.40
IUUC-Tni-119937	Tetraodon nigroviridis	27.45	7.00e-31	125.00
IUUC-Tca-121323	Theobroma cacao	30.98	3.00e-40	157.00
IUUC-Tre-121993	Trichoderma reesei	56.87	2.00e-92	330.00
IUUC-Tvi-122332	Trichoderma virens	53.63	3.00e-92	329.00
IUUC-Tae-123478	Triticum aestivum	35.94	9.00e-33	132.00
IUUC-Tur-126679	Triticum urartu	35.55	3.00e-32	130.00
IUUC-Tme-127159	Tuber melanosporum	48.65	2.00e-19	87.40
IUUC-Tbe-127590	Tupaia belangeri	25.26	1.60e-02	31.20
IUUC-Ttr-128261	Tursiops truncatus	35.63	8.00e-35	139.00
IUUC-Uma-129572	Ustilago maydis	33.17	2.00e-24	105.00
IUUC-Vda-129966	Verticillium dahliae	64.99	2.00e-111	393.00
IUUC-Vpa-130698	Vicugna pacos	31.37	8.00e-25	105.00
IUUC-Vvi-131240	Vitis vinifera	25.00	2.00e-03	34.30
IUUC-Xtr-132878	Xenopus tropicalis	37.17	2.00e-35	140.00
IUUC-Xma-134017	Xiphophorus maculatus	32.07	6.00e-20	89.40
IUUC-Yli-134373	Yarrowia lipolytica	40.17	3.00e-54	203.00
IUUC-Zma-135222	Zea mays	53.16	3.00e-19	87.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence