iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Cne-026905

Ensembl Protein ID

AAW46088

UniProt Accession

P0CM34; Q55JM5; Q5K9X6; ATG3_CRYNJ

Genbank Protein ID

AAW46088.1

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme ATG3

Genbank Nucleotide ID

AE017351

Gene Name

ATG3; CNK00360

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
CNK00360	AAW46088	AAW46088

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	37.41	4.00e-42	169.00	IUUC-Ath-006802
E2/Autophagy_C	39.13	2.00e-47	187.00	IUUC-Hsa-046449
E2/Autophagy_C	49.37	2.00e-17	84.70	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
277	Glycyl thioester intermediate	{ECO:0000250}

Organism

Cryptococcus neoformans

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNNPLLAIQS QYWAVRDYLS PVLRESKFKE HGRITPEEFV AAGDFLTFKF PVWQWEKGES 60
SRARDFLPPD KQYLVTRNVP CLRRATAVDY TNADEDAEKL LSFLDDAEEA PGPDDDWVAT 120

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Nucleotide Sequence
(Fasta)

ATGAACAACC CACTTTTAGC TATCCAAGTA AGTCCTAGCA CGAGTTTTGA TAAATGCACT 60
GCTAATCCAT ACATGTAGAG TCAGTACTGG GCTGTAAGAG ACTACCTCTC TCCGGTACGC 120

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Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0000422--P:mitophagy
GO:0044804--P:nucleophagy
GO:0015031--P:protein transport

KEGG

cne:CNK00360

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	38.81	5.00e-45	172.00
IUUC-Aml-002081	Ailuropoda melanoleuca	39.40	6.00e-52	195.00
IUUC-Atr-002712	Amborella trichopoda	37.37	7.00e-44	168.00
IUUC-Apl-003956	Anas platyrhynchos	33.22	3.00e-30	123.00
IUUC-Aca-004578	Anolis carolinensis	40.20	4.00e-52	196.00
IUUC-Aly-005823	Arabidopsis lyrata	34.25	1.00e-47	181.00
IUUC-Ath-006802	Arabidopsis thaliana	34.52	1.00e-48	184.00
IUUC-Ago-007855	Ashbya gossypii	30.35	5.00e-38	149.00
IUUC-Acl-008147	Aspergillus clavatus	37.57	4.00e-22	96.70
IUUC-Afl-008483	Aspergillus flavus	46.73	2.00e-20	91.30
IUUC-Afu-008818	Aspergillus fumigatus	36.17	3.00e-25	107.00
IUUC-Ani-009481	Aspergillus nidulans	35.11	5.00e-21	93.20
IUUC-Ang-009800	Aspergillus niger	35.00	6.00e-21	92.80
IUUC-Aor-010150	Aspergillus oryzae	46.73	2.00e-20	91.30
IUUC-Ate-010545	Aspergillus terreus	34.59	2.00e-19	87.40
IUUC-Ame-011793	Astyanax mexicanus	39.00	7.00e-52	195.00
IUUC-Bgr-012167	Blumeria graminis	34.62	3.00e-18	84.00
IUUC-Bta-012695	Bos taurus	39.67	2.00e-52	197.00
IUUC-Bci-013972	Botrytis cinerea	43.93	5.00e-19	86.70
IUUC-Bdi-014756	Brachypodium distachyon	33.80	7.00e-45	172.00
IUUC-Bol-015091	Brassica oleracea	34.89	4.00e-45	172.00
IUUC-Bra-017220	Brassica rapa	34.25	9.00e-47	178.00
IUUC-Cel-018143	Caenorhabditis elegans	42.98	4.00e-23	99.80
IUUC-Cja-018991	Callithrix jacchus	39.00	5.00e-53	199.00
IUUC-Cfa-020070	Canis familiaris	39.59	4.00e-51	192.00
IUUC-Cpo-021860	Cavia porcellus	39.00	3.00e-52	196.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	52.27	2.00e-25	107.00
IUUC-Csa-023881	Chlorocebus sabaeus	39.33	6.00e-52	195.00
IUUC-Cho-024852	Choloepus hoffmanni	37.33	4.00e-50	189.00
IUUC-Cin-025787	Ciona intestinalis	37.25	8.00e-52	195.00
IUUC-Csv-026369	Ciona savignyi	39.46	2.00e-50	190.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	57.75	7.00e-21	92.80
IUUC-Dre-028811	Danio rerio	38.16	7.00e-51	192.00
IUUC-Dno-030081	Dasypus novemcinctus	39.20	4.00e-49	186.00
IUUC-Dor-030183	Dipodomys ordii	43.09	1.00e-25	108.00
IUUC-Dse-031539	Dothistroma septosporum	45.05	3.00e-14	70.50
IUUC-Dme-031805	Drosophila melanogaster	39.12	8.00e-51	191.00
IUUC-Ete-033171	Echinops telfairi	39.33	2.00e-51	194.00
IUUC-Eca-034083	Equus caballus	39.67	2.00e-52	197.00
IUUC-Eeu-034804	Erinaceus europaeus	39.33	1.00e-51	194.00
IUUC-Fca-036289	Felis catus	39.00	7.00e-52	195.00
IUUC-Fal-037060	Ficedula albicollis	38.80	1.00e-51	194.00
IUUC-Fox-038051	Fusarium oxysporum	42.34	2.00e-22	97.80
IUUC-Fso-038124	Fusarium solani	41.61	2.00e-22	97.80
IUUC-Gmo-039538	Gadus morhua	38.51	4.00e-50	189.00
IUUC-Ggr-039880	Gaeumannomyces graminis	34.44	2.00e-21	94.40
IUUC-Gga-041108	Gallus gallus	39.26	5.00e-51	192.00
IUUC-Gac-041558	Gasterosteus aculeatus	40.07	1.00e-51	194.00
IUUC-Gma-043969	Glycine max	34.53	1.00e-46	177.00
IUUC-Ggo-045071	Gorilla gorilla	39.00	8.00e-52	195.00
IUUC-Hsa-046449	Homo sapiens	39.00	8.00e-52	195.00
IUUC-Hvu-047451	Hordeum vulgare	36.29	2.00e-47	181.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	39.74	9.00e-52	194.00
IUUC-Kpa-049096	Komagataella pastoris	53.85	2.00e-21	94.40
IUUC-Lch-049726	Latimeria chalumnae	36.67	1.00e-52	198.00
IUUC-Lpe-050734	Leersia perrieri	40.88	2.00e-47	181.00
IUUC-Loc-051983	Lepisosteus oculatus	38.87	1.00e-52	197.00
IUUC-Lma-053038	Leptosphaeria maculans	42.98	2.00e-18	84.30
IUUC-Laf-054510	Loxodonta africana	40.43	1.00e-46	177.00
IUUC-Mcc-054792	Macaca mulatta	39.33	6.00e-52	195.00
IUUC-Meu-055965	Macropus eugenii	32.56	2.00e-34	137.00
IUUC-Mor-057292	Magnaporthe oryzae	42.34	7.00e-23	99.40
IUUC-Mtr-058282	Medicago truncatula	34.08	3.00e-48	183.00
IUUC-Mla-059144	Melampsora laricipopulina	49.22	2.00e-93	333.00
IUUC-Mga-059509	Meleagris gallopavo	39.46	1.00e-51	194.00
IUUC-Mvi-060281	Microbotryum violaceum	43.73	7.00e-73	265.00
IUUC-Mmr-061788	Microcebus murinus	39.33	3.00e-52	196.00
IUUC-Mdo-062922	Monodelphis domestica	39.67	7.00e-52	195.00
IUUC-Mmu-064249	Mus musculus	39.67	5.00e-53	199.00
IUUC-Mac-064843	Musa acuminata	35.71	2.00e-50	190.00
IUUC-Mpu-065717	Mustela putorius furo	38.67	1.00e-51	194.00
IUUC-Mlu-067151	Myotis lucifugus	38.38	4.00e-44	169.00
IUUC-Nfi-068551	Neosartorya fischeri	37.97	4.00e-25	106.00
IUUC-Ncr-068846	Neurospora crassa	35.26	4.00e-37	146.00
IUUC-Nle-069790	Nomascus leucogenys	39.00	8.00e-52	195.00
IUUC-Ont-072517	Oreochromis niloticus	37.12	2.00e-49	187.00
IUUC-Oan-073285	Ornithorhynchus anatinus	45.30	1.00e-25	107.00
IUUC-Ocu-074695	Oryctolagus cuniculus	39.00	1.00e-51	194.00
IUUC-Oba-075515	Oryza barthii	36.77	2.00e-50	190.00
IUUC-Obr-076787	Oryza brachyantha	36.03	8.00e-50	188.00
IUUC-Ogl-077440	Oryza glaberrima	36.69	3.00e-51	193.00
IUUC-Ogu-078037	Oryza glumaepatula	37.29	4.00e-50	189.00
IUUC-Oin-079452	Oryza indica	35.73	3.00e-50	189.00
IUUC-Olo-080450	Oryza longistaminata	35.39	2.00e-47	181.00
IUUC-Ome-081514	Oryza meridionalis	36.41	8.00e-51	191.00
IUUC-Oni-082677	Oryza nivara	35.73	3.00e-50	189.00
IUUC-Opu-083925	Oryza punctata	36.69	7.00e-51	192.00
IUUC-Oru-084828	Oryza rufipogon	34.63	2.00e-47	180.00
IUUC-Osa-085937	Oryza sativa	35.73	3.00e-50	190.00
IUUC-Ola-086559	Oryzias latipes	38.43	1.00e-36	144.00
IUUC-Olu-087766	Ostreococcus lucimarinus	52.08	9.00e-29	118.00
IUUC-Oga-087983	Otolemur garnettii	39.20	4.00e-51	192.00
IUUC-Oar-089281	Ovis aries	40.00	2.00e-52	197.00
IUUC-Ptr-090983	Pan troglodytes	39.00	8.00e-52	195.00
IUUC-Pan-092457	Papio anubis	39.33	5.00e-52	196.00
IUUC-Psi-094046	Pelodiscus sinensis	37.59	2.00e-44	170.00
IUUC-Pno-094925	Phaeosphaeria nodorum	43.48	4.00e-19	86.70
IUUC-Ppa-095487	Physcomitrella patens	33.79	7.00e-46	175.00
IUUC-Pfo-096968	Poecilia formosa	39.27	5.00e-51	192.00
IUUC-Pab-098621	Pongo abelii	38.67	2.00e-49	187.00
IUUC-Pop-100007	Populus trichocarpa	34.07	5.00e-47	179.00
IUUC-Pca-100590	Procavia capensis	46.15	2.00e-26	111.00
IUUC-Ppe-101657	Prunus persica	34.65	8.00e-49	185.00
IUUC-Pva-102446	Pteropus vampyrus	39.33	7.00e-52	195.00
IUUC-Pgr-103604	Puccinia graminis	49.32	2.00e-73	267.00
IUUC-Ptt-103941	Puccinia triticina	57.25	1.00e-41	162.00
IUUC-Pte-104252	Pyrenophora teres	42.98	1.00e-18	85.10
IUUC-Pyt-104556	Pyrenophora triticirepentis	42.98	1.00e-18	85.10
IUUC-Rno-106025	Rattus norvegicus	40.33	2.00e-52	196.00
IUUC-Sce-106172	Saccharomyces cerevisiae	31.43	2.00e-33	133.00
IUUC-Sha-106858	Sarcophilus harrisii	40.20	7.00e-52	195.00
IUUC-Sja-107905	Schizosaccharomyces japonicus	48.31	5.00e-19	86.30
IUUC-Spo-108185	Schizosaccharomyces pombe	48.15	2.00e-18	84.30
IUUC-Smo-108883	Selaginella moellendorffii	34.08	2.00e-41	160.00
IUUC-Sit-110512	Setaria italica	40.57	3.00e-29	120.00
IUUC-Sly-111238	Solanum lycopersicum	34.71	5.00e-49	185.00
IUUC-Stu-112441	Solanum tuberosum	35.49	2.00e-49	187.00
IUUC-Sar-113039	Sorex araneus	45.30	7.00e-26	108.00
IUUC-Sbi-113776	Sorghum bicolor	35.16	1.00e-46	177.00
IUUC-Sre-115074	Sporisorium reilianum	45.24	3.00e-66	243.00
IUUC-Ssc-115262	Sus scrofa	38.82	2.00e-52	197.00
IUUC-Tgu-116790	Taeniopygia guttata	38.80	3.00e-51	193.00
IUUC-Tru-118160	Takifugu rubripes	38.51	1.00e-52	198.00
IUUC-Tsy-119145	Tarsius syrichta	34.15	1.00e-27	115.00
IUUC-Tni-119937	Tetraodon nigroviridis	36.88	3.00e-44	169.00
IUUC-Tca-121323	Theobroma cacao	35.34	4.00e-48	182.00
IUUC-Tre-121993	Trichoderma reesei	57.75	1.00e-20	91.70
IUUC-Tvi-122332	Trichoderma virens	56.34	2.00e-20	91.30
IUUC-Tae-123478	Triticum aestivum	34.90	4.00e-47	179.00
IUUC-Tur-126679	Triticum urartu	35.18	6.00e-47	179.00
IUUC-Tme-127159	Tuber melanosporum	39.24	3.00e-13	67.00
IUUC-Ttr-128261	Tursiops truncatus	39.86	2.00e-46	177.00
IUUC-Uma-129572	Ustilago maydis	42.47	5.00e-80	289.00
IUUC-Vda-129966	Verticillium dahliae	57.75	6.00e-21	92.80
IUUC-Vpa-130698	Vicugna pacos	34.67	3.00e-40	156.00
IUUC-Xtr-132878	Xenopus tropicalis	38.49	8.00e-51	191.00
IUUC-Xma-134017	Xiphophorus maculatus	39.67	5.00e-50	189.00
IUUC-Yli-134373	Yarrowia lipolytica	42.74	4.00e-22	96.70
IUUC-Zma-135222	Zea mays	34.54	4.00e-46	176.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence