iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-063248

UUCD1 version

UUC-MuM-00704

Ensembl Protein ID

ENSMUSP00000110425.2

UniProt Accession

Q8C5D8; O54987; Q8C384; Q8CDQ8; Q8K208; Q99JX5; Q9D5W7; Q9QZ63; PIAS2_MOUSE

Genbank Protein ID

AAF12825.1; BAC26579.1; BAB29594.1; BAC37407.1; BAC39710.1; AAH05596.1; AAH34711.1; AAB96678.1

Protein Name

E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; DAB2-interacting protein; Msx-interacting zinc finger protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2; RING-type E3 ubiquitin transferase PIAS2

Genbank Nucleotide ID

AF201391; AK029716; AK014871; AK078813; AK086653; BC005596; BC034711; AF039567

Gene Name

Pias2; Miz1; Piasx

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000025423.15	ENSMUST00000114777.8	ENSMUSP00000110425.2
ENSMUSG00000025423.15	ENSMUST00000168882.7	ENSMUSP00000131485.1
ENSMUSG00000025423.15	ENSMUST00000114776.2	ENSMUSP00000110424.2

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	microRNA	TRANSFAC	RepTar
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus	dbPTM	UniProt
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E3 activity/RING/RING	98.07	0.00e+00	1040.00	IUUC-Hsa-045854

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Organism

Mus musculus

Functional Description
(View)

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).

Protein Sequence
(Fasta)

MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS PAVQIKIREL 60
YRRRYPRTLE GLCDLSTIKS SVFSLDGSSS PVEPDLPVAG IHSLPSTSIT PHSPSSPVGS 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCGCAGGTCA CGTGAGGGGC CCGGTGGAGG TGGTGGAGGT GTCGGCGCGG CGGCGGCGGC 60
GGCGGCGGCG GCGGCCGCGG CGTCTAGAGC GGCGCCCAGT GCAGGATGTT GCAGGAGACG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR027228--PIAS2
IPR023321--PINIT
IPR003034--SAP_dom
IPR004181--Znf_MIZ

PROSITE

PS51466--PINIT
PS50800--SAP
PS51044--ZF_SP_RING

Pfam

PF14324--PINIT
PF02891--zf-MIZ

SMART

SM00513--SAP

Gene Ontology

GO:0016604--C:nuclear body
GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016605--C:PML body
GO:0050681--F:androgen receptor binding
GO:0003677--F:DNA binding
GO:0061665--F:SUMO ligase activity
GO:0019789--F:SUMO transferase activity
GO:0008134--F:transcription factor binding
GO:0031625--F:ubiquitin protein ligase binding
GO:0008270--F:zinc ion binding
GO:0060766--P:negative regulation of androgen receptor signaling pathway
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0016925--P:protein sumoylation
GO:0060765--P:regulation of androgen receptor signaling pathway
GO:0045667--P:regulation of osteoblast differentiation
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated

KEGG

mmu:17344

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002262	Ailuropoda melanoleuca	96.46	0.00e+00	1010.00
IUUC-Apl-004116	Anas platyrhynchos	91.87	0.00e+00	931.00
IUUC-Aca-004464	Anolis carolinensis	90.75	0.00e+00	954.00
IUUC-Acl-008204	Aspergillus clavatus	34.21	3.00e-28	119.00
IUUC-Afl-008672	Aspergillus flavus	37.36	2.00e-29	123.00
IUUC-Afu-008758	Aspergillus fumigatus	30.95	7.00e-30	125.00
IUUC-Ani-009389	Aspergillus nidulans	34.78	2.00e-26	114.00
IUUC-Ang-009590	Aspergillus niger	36.78	1.00e-28	121.00
IUUC-Aor-009912	Aspergillus oryzae	40.60	4.00e-25	109.00
IUUC-Ate-010496	Aspergillus terreus	37.08	1.00e-29	124.00
IUUC-Ame-010633	Astyanax mexicanus	69.47	0.00e+00	787.00
IUUC-Bgr-012177	Blumeria graminis	28.49	6.00e-24	105.00
IUUC-Bta-013489	Bos taurus	95.02	0.00e+00	1017.00
IUUC-Cja-019247	Callithrix jacchus	98.07	0.00e+00	1026.00
IUUC-Cfa-021020	Canis familiaris	97.55	0.00e+00	1010.00
IUUC-Csa-023988	Chlorocebus sabaeus	98.18	0.00e+00	1006.00
IUUC-Cho-024816	Choloepus hoffmanni	86.13	0.00e+00	835.00
IUUC-Dre-027615	Danio rerio	71.88	0.00e+00	791.00
IUUC-Dno-028983	Dasypus novemcinctus	97.28	0.00e+00	939.00
IUUC-Dor-030560	Dipodomys ordii	78.96	0.00e+00	707.00
IUUC-Dse-031254	Dothistroma septosporum	31.86	2.00e-27	117.00
IUUC-Ete-032543	Echinops telfairi	80.42	0.00e+00	786.00
IUUC-Eca-033818	Equus caballus	98.04	0.00e+00	1006.00
IUUC-Eeu-035256	Erinaceus europaeus	83.69	0.00e+00	830.00
IUUC-Fca-035422	Felis catus	97.55	0.00e+00	1006.00
IUUC-Fal-037641	Ficedula albicollis	89.87	0.00e+00	1000.00
IUUC-Ggr-039896	Gaeumannomyces graminis	34.84	8.00e-29	121.00
IUUC-Gga-040849	Gallus gallus	90.51	0.00e+00	996.00
IUUC-Ggo-044666	Gorilla gorilla	96.94	0.00e+00	1007.00
IUUC-Hsa-045854	Homo sapiens	98.07	0.00e+00	1028.00
IUUC-Hvu-047670	Hordeum vulgare	38.46	6.00e-17	82.80
IUUC-Lch-050424	Latimeria chalumnae	74.03	0.00e+00	771.00
IUUC-Loc-052343	Lepisosteus oculatus	77.45	0.00e+00	812.00
IUUC-Laf-054247	Loxodonta africana	95.64	0.00e+00	985.00
IUUC-Mcc-055103	Macaca mulatta	98.20	0.00e+00	1010.00
IUUC-Meu-056533	Macropus eugenii	68.18	0.00e+00	696.00
IUUC-Mla-058882	Melampsora laricipopulina	32.66	1.00e-23	104.00
IUUC-Mmr-060682	Microcebus murinus	97.58	0.00e+00	1045.00
IUUC-Mdo-062100	Monodelphis domestica	87.68	0.00e+00	992.00
IUUC-Mpu-066494	Mustela putorius furo	97.39	0.00e+00	1009.00
IUUC-Mlu-067038	Myotis lucifugus	96.93	0.00e+00	1003.00
IUUC-Nfi-068586	Neosartorya fischeri	35.14	5.00e-30	125.00
IUUC-Nle-070060	Nomascus leucogenys	98.18	0.00e+00	985.00
IUUC-Opr-070251	Ochotona princeps	77.98	0.00e+00	816.00
IUUC-Ont-071330	Oreochromis niloticus	74.48	0.00e+00	652.00
IUUC-Oan-073028	Ornithorhynchus anatinus	94.56	0.00e+00	1019.00
IUUC-Ocu-074400	Oryctolagus cuniculus	95.44	0.00e+00	1017.00
IUUC-Ola-087125	Oryzias latipes	67.56	4.00e-180	624.00
IUUC-Oga-089150	Otolemur garnettii	95.34	0.00e+00	1005.00
IUUC-Oar-089866	Ovis aries	95.47	0.00e+00	1014.00
IUUC-Ptr-090464	Pan troglodytes	97.58	0.00e+00	1022.00
IUUC-Pan-091938	Papio anubis	98.23	0.00e+00	1030.00
IUUC-Psi-093868	Pelodiscus sinensis	94.13	0.00e+00	969.00
IUUC-Pno-094862	Phaeosphaeria nodorum	33.49	8.00e-30	125.00
IUUC-Pfo-097230	Poecilia formosa	70.64	0.00e+00	626.00
IUUC-Pab-097699	Pongo abelii	98.07	0.00e+00	1028.00
IUUC-Pca-101088	Procavia capensis	87.60	0.00e+00	882.00
IUUC-Pgr-103580	Puccinia graminis	34.57	2.00e-24	107.00
IUUC-Ptt-103870	Puccinia triticina	32.56	8.00e-26	110.00
IUUC-Sha-107323	Sarcophilus harrisii	89.74	0.00e+00	1024.00
IUUC-Sar-113273	Sorex araneus	96.25	0.00e+00	1083.00
IUUC-Tgu-116644	Taeniopygia guttata	89.39	0.00e+00	984.00
IUUC-Tru-117897	Takifugu rubripes	66.33	0.00e+00	631.00
IUUC-Tsy-119019	Tarsius syrichta	86.28	0.00e+00	865.00
IUUC-Tni-120384	Tetraodon nigroviridis	71.36	2.00e-179	622.00
IUUC-Tme-126880	Tuber melanosporum	29.29	3.00e-27	116.00
IUUC-Tbe-127942	Tupaia belangeri	80.00	4.00e-137	481.00
IUUC-Ttr-128980	Tursiops truncatus	77.16	0.00e+00	696.00
IUUC-Vda-129985	Verticillium dahliae	35.65	1.00e-30	127.00
IUUC-Vpa-130725	Vicugna pacos	96.65	0.00e+00	842.00
IUUC-Xtr-132733	Xenopus tropicalis	77.49	0.00e+00	881.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence