iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-063405

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

NEDD8-conjugating enzyme UBE2F-B; NEDD8 carrier protein UBE2F-B; NEDD8 protein ligase UBE2F-B; RING-type E3 NEDD8 transferase UBE2F-B; Ubiquitin-conjugating enzyme E2 F-B

Genbank Nucleotide ID

AK136183

Gene Name

Gm5434; Ube2fb

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000059301.5	ENSMUST00000071825.5	ENSMUSP00000071728.5

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	microRNA	TRANSFAC	RAID2
Post-translational Modifications (PTMs)
CPLM
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	1.40e-35	123.2	38	177

Active Site

Position(s)	Description	Evidence
116	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 3    kkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilk.... 87
             e++ele++ p ++++++ d ++l+ +++++  p++ +Y+gg F++eie+p+ Y++ PPkvk+ltki+hPn++e+G++Cls+l+    
   Q: 38   VTEVAELEANLPWTCKVHFPDPNKLHCFQLTVS-PDEGYYQGGNFQFEIEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLRehsa 125
           679*******************99********9.**************************************************76777 PP
   S: 88   eeekWspalsvesvllsiqsllae.pnpesplneeaaellkknreeykkkvr 138
           + + W p+ ++++v+++++sl+++  n+ +pln eaae++ +++e+++ kv+
   Q: 126  DGTGWAPTRTLKDVVWGLNSLFTDlLNFYDPLNIEAAEHHLQDKEDFRDKVD 177
           8899******************8879**********************9996 PP

Organism

Mus musculus

Functional Description
(View)

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Protein Sequence
(Fasta)

MLTLASKLKW DEDLKGSQTS VSTSDSTRRF SMRDKLLVTE VAELEANLPW TCKVHFPDPN 60
KLHCFQLTVS PDEGYYQGGN FQFEIEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCGGCTCTGA AGGGCTGCGC AACTCGGTGC CGCTGCTCGC CGGGCACGGT TTTGGGTGCC 60
GGCTCACCTC TCCTGATTCG TGGTGCTCAG AAAGGTGGCA GTAATGCTAA CGCTGGCAAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005524--F:ATP binding
GO:0019788--F:NEDD8 transferase activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0045116--P:protein neddylation

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence