iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Gac-042463

UUCD1 version

UUC-GaA-00688

Ensembl Protein ID

ENSGACP00000017824.1

UniProt Accession

G3PJP4; G3PJP4_GASAC

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSGACG00000013471.1	ENSGACT00000017859.1	ENSGACP00000017824.1
ENSGACG00000013471.1	ENSGACT00000017871.1	ENSGACP00000017836.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	1.80e-08	35.2	381	419

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+ +C+++w       +k++ CP+Cr
   Q: 381  CIICSELFIE-AVILSCAHSFCSHCIKQW------RRKKDECPICR 419
           ********99.888***************......**********8 PP

Organism

Gasterosteus aculeatus

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MDTVAADSSP AEEDESSDSE VLCLKRVGRN SDWLRLLGNT EIRIGRGLDV THQLLSPSCP 60
LMISRLHCTV RQREDGQWTV TDEKSLNGVW VNGNRIPAEE AHQLRLGDSI QLGVPVTGTE 120

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Nucleotide Sequence
(Fasta)

CACCATGTAG AACAACACGG CTTCAGCTCC TTGCAGCCGC TGGCGGAATG AGATAAAAAT 60
GGACACCGTG GCAGCGGACT CGTCCCCGGC CGAGGAGGAC GAGTCTTCGG ACTCTGAGGT 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	39.53	8.00e-62	231.00
IUUC-Atr-002751	Amborella trichopoda	37.11	5.00e-07	48.50
IUUC-Apl-003664	Anas platyrhynchos	64.77	2.00e-30	124.00
IUUC-Aca-005036	Anolis carolinensis	39.44	7.00e-64	238.00
IUUC-Ame-011537	Astyanax mexicanus	53.42	3.00e-118	418.00
IUUC-Bta-013297	Bos taurus	40.47	1.00e-63	237.00
IUUC-Cja-019201	Callithrix jacchus	39.19	5.00e-70	258.00
IUUC-Cfa-021041	Canis familiaris	39.74	2.00e-69	256.00
IUUC-Cre-022788	Chlamydomonas reinhardtii	29.17	4.00e-10	58.90
IUUC-Csa-024133	Chlorocebus sabaeus	41.19	5.00e-71	261.00
IUUC-Cho-024624	Choloepus hoffmanni	33.33	6.00e-28	118.00
IUUC-Dre-027918	Danio rerio	53.39	6.00e-117	414.00
IUUC-Dno-029774	Dasypus novemcinctus	40.08	3.00e-72	266.00
IUUC-Dor-030190	Dipodomys ordii	34.62	2.00e-42	166.00
IUUC-Ete-032301	Echinops telfairi	56.89	2.00e-40	159.00
IUUC-Eca-033607	Equus caballus	40.00	2.00e-58	219.00
IUUC-Eeu-034888	Erinaceus europaeus	47.41	2.00e-45	176.00
IUUC-Fca-036075	Felis catus	42.17	2.00e-71	262.00
IUUC-Fal-037569	Ficedula albicollis	58.33	3.00e-45	176.00
IUUC-Gmo-038588	Gadus morhua	60.77	5.00e-143	500.00
IUUC-Gga-040777	Gallus gallus	42.83	3.00e-72	266.00
IUUC-Ggo-045269	Gorilla gorilla	40.89	1.00e-71	263.00
IUUC-Hsa-046249	Homo sapiens	41.40	1.00e-67	250.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	39.75	4.00e-72	265.00
IUUC-Lch-049805	Latimeria chalumnae	43.91	8.00e-82	297.00
IUUC-Loc-052377	Lepisosteus oculatus	49.04	6.00e-103	367.00
IUUC-Mcc-055344	Macaca mulatta	40.68	3.00e-70	259.00
IUUC-Meu-055950	Macropus eugenii	40.09	4.00e-62	231.00
IUUC-Mla-059052	Melampsora laricipopulina	48.84	2.00e-09	55.50
IUUC-Mga-060049	Meleagris gallopavo	60.84	2.00e-46	180.00
IUUC-Mmr-061431	Microcebus murinus	38.92	2.00e-64	239.00
IUUC-Mdo-062016	Monodelphis domestica	59.35	7.00e-37	150.00
IUUC-Mmu-063368	Mus musculus	37.22	4.00e-68	252.00
IUUC-Mpu-066449	Mustela putorius furo	40.38	3.00e-73	268.00
IUUC-Mlu-067588	Myotis lucifugus	42.04	4.00e-61	228.00
IUUC-Nle-069192	Nomascus leucogenys	40.68	2.00e-71	263.00
IUUC-Opr-070300	Ochotona princeps	32.63	3.00e-50	192.00
IUUC-Ont-071998	Oreochromis niloticus	74.48	5.00e-167	580.00
IUUC-Oan-073610	Ornithorhynchus anatinus	52.98	2.00e-39	155.00
IUUC-Ocu-074603	Oryctolagus cuniculus	39.63	1.00e-70	260.00
IUUC-Ola-086681	Oryzias latipes	64.35	8.00e-158	550.00
IUUC-Oga-088183	Otolemur garnettii	40.13	3.00e-71	262.00
IUUC-Oar-090255	Ovis aries	40.18	8.00e-58	217.00
IUUC-Ptr-091029	Pan troglodytes	41.21	2.00e-68	252.00
IUUC-Pan-092241	Papio anubis	40.68	6.00e-71	261.00
IUUC-Psi-093506	Pelodiscus sinensis	39.29	7.00e-62	231.00
IUUC-Pma-094579	Petromyzon marinus	52.22	1.00e-24	107.00
IUUC-Pfo-097014	Poecilia formosa	77.66	1.00e-166	579.00
IUUC-Pab-098530	Pongo abelii	39.22	1.00e-67	250.00
IUUC-Pca-100939	Procavia capensis	33.19	1.00e-38	154.00
IUUC-Pva-103148	Pteropus vampyrus	37.34	1.00e-61	230.00
IUUC-Rno-105338	Rattus norvegicus	40.08	2.00e-72	266.00
IUUC-Sha-107187	Sarcophilus harrisii	59.88	5.00e-45	174.00
IUUC-Sar-113356	Sorex araneus	36.92	2.00e-45	176.00
IUUC-Ssc-116290	Sus scrofa	39.70	1.00e-74	273.00
IUUC-Tru-117910	Takifugu rubripes	73.75	2.00e-172	598.00
IUUC-Tsy-119329	Tarsius syrichta	33.97	1.00e-50	193.00
IUUC-Tni-120521	Tetraodon nigroviridis	70.96	2.00e-158	551.00
IUUC-Ttr-128997	Tursiops truncatus	35.03	2.00e-51	196.00
IUUC-Vpa-130154	Vicugna pacos	36.40	5.00e-65	241.00
IUUC-Xtr-132805	Xenopus tropicalis	42.07	1.00e-71	264.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence