iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Tru-117910

UUCD1 version

UUC-TaR-00279

Ensembl Protein ID

ENSTRUP00000014191.1

UniProt Accession

H2SP54; H2SP54_TAKRU

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSTRUG00000005824.1	ENSTRUT00000014256.1	ENSTRUP00000014191.1
ENSTRUG00000005824.1	ENSTRUT00000014257.1	ENSTRUP00000014192.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	8.20e-07	30.7	378	416

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+  C+++w       +k++ CP+Cr
   Q: 378  CIICSELFIE-AVILNCAHSFCCYCIKQW------RKKKDECPICR 416
           ********99.888***************......**********8 PP

Organism

Takifugu rubripes

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MESVATNSSV AEEEDDGDSD VSCLMRVGRN SDWLRFYENS EITVGRGLDV TYQLLSPSCP 60
LMISRLHCTF RQRDDGQWTV TDKKSLNGVW VNGSRIPAEE AHQLKLGDSI QLGVPIIGSE 120

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Nucleotide Sequence
(Fasta)

ATTTCGGATA TGGATGTGGG GACGGTCTTT AACATTAGGT GCTGCAGGTG TAAATAGACC 60
CCTAAAACAT AAAATAAGAT GGAGAGTGTG GCGACCAATT CTTCTGTGGC TGAGGAGGAA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR011991--WHTH_DNA-bd_dom
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	40.65	2.00e-61	230.00
IUUC-Apl-003664	Anas platyrhynchos	61.54	6.00e-32	129.00
IUUC-Aca-005036	Anolis carolinensis	41.35	3.00e-64	239.00
IUUC-Ame-011537	Astyanax mexicanus	50.52	7.00e-110	390.00
IUUC-Bta-013297	Bos taurus	62.86	9.00e-37	147.00
IUUC-Cja-019201	Callithrix jacchus	59.04	1.00e-39	157.00
IUUC-Cfa-021041	Canis familiaris	58.33	2.00e-40	160.00
IUUC-Cre-022788	Chlamydomonas reinhardtii	27.84	3.00e-09	56.20
IUUC-Csa-024133	Chlorocebus sabaeus	57.74	3.00e-39	155.00
IUUC-Cho-024624	Choloepus hoffmanni	45.93	7.00e-22	98.20
IUUC-Cne-026875	Cryptococcus neoformans	30.77	2.00e-06	47.40
IUUC-Dre-027918	Danio rerio	51.21	4.00e-107	382.00
IUUC-Dno-029774	Dasypus novemcinctus	39.42	2.00e-66	246.00
IUUC-Dor-030190	Dipodomys ordii	33.19	7.00e-45	174.00
IUUC-Ete-032301	Echinops telfairi	53.89	3.00e-41	162.00
IUUC-Eca-033607	Equus caballus	40.97	1.00e-57	217.00
IUUC-Eeu-034888	Erinaceus europaeus	53.37	5.00e-40	158.00
IUUC-Fca-036075	Felis catus	53.19	2.00e-28	120.00
IUUC-Fal-037569	Ficedula albicollis	57.07	3.00e-45	176.00
IUUC-Gmo-038588	Gadus morhua	56.33	4.00e-130	458.00
IUUC-Gga-040777	Gallus gallus	42.16	2.00e-70	259.00
IUUC-Gac-042463	Gasterosteus aculeatus	74.83	4.00e-157	547.00
IUUC-Ggo-045269	Gorilla gorilla	39.79	4.00e-64	238.00
IUUC-Hsa-046249	Homo sapiens	39.87	2.00e-61	229.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	39.62	5.00e-69	254.00
IUUC-Lch-049805	Latimeria chalumnae	43.31	2.00e-82	299.00
IUUC-Loc-052377	Lepisosteus oculatus	48.83	2.00e-102	365.00
IUUC-Mcc-055344	Macaca mulatta	57.74	3.00e-39	155.00
IUUC-Meu-055950	Macropus eugenii	38.05	3.00e-59	222.00
IUUC-Mla-059052	Melampsora laricipopulina	51.16	4.00e-09	54.70
IUUC-Mga-060049	Meleagris gallopavo	60.84	6.00e-45	175.00
IUUC-Mmr-061431	Microcebus murinus	65.71	2.00e-38	153.00
IUUC-Mdo-062016	Monodelphis domestica	61.94	3.00e-38	154.00
IUUC-Mmu-063235	Mus musculus	54.76	2.00e-47	182.00
IUUC-Mpu-066449	Mustela putorius furo	40.56	1.00e-69	257.00
IUUC-Mlu-067588	Myotis lucifugus	40.99	3.00e-62	232.00
IUUC-Nle-069192	Nomascus leucogenys	39.57	8.00e-64	238.00
IUUC-Opr-070300	Ochotona princeps	56.38	7.00e-30	125.00
IUUC-Ont-071998	Oreochromis niloticus	69.41	3.00e-157	548.00
IUUC-Oan-073610	Ornithorhynchus anatinus	50.60	4.00e-42	164.00
IUUC-Ocu-074603	Oryctolagus cuniculus	60.25	7.00e-41	161.00
IUUC-Ola-086681	Oryzias latipes	61.85	4.00e-145	508.00
IUUC-Oga-088183	Otolemur garnettii	58.68	8.00e-41	161.00
IUUC-Oar-090255	Ovis aries	61.27	9.00e-36	144.00
IUUC-Ptr-091029	Pan troglodytes	39.22	1.00e-59	223.00
IUUC-Pan-092241	Papio anubis	58.33	7.00e-40	158.00
IUUC-Psi-093506	Pelodiscus sinensis	63.07	3.00e-45	176.00
IUUC-Pma-094579	Petromyzon marinus	53.85	2.00e-24	107.00
IUUC-Pfo-097014	Poecilia formosa	72.29	1.00e-155	543.00
IUUC-Pab-098530	Pongo abelii	40.17	3.00e-63	235.00
IUUC-Pca-100939	Procavia capensis	32.03	2.00e-36	146.00
IUUC-Pva-103148	Pteropus vampyrus	36.23	3.00e-57	216.00
IUUC-Rno-105338	Rattus norvegicus	56.29	2.00e-48	186.00
IUUC-Sha-107187	Sarcophilus harrisii	61.76	4.00e-46	178.00
IUUC-Sar-113356	Sorex araneus	62.00	1.00e-37	150.00
IUUC-Ssc-116290	Sus scrofa	40.51	6.00e-73	268.00
IUUC-Tsy-119329	Tarsius syrichta	38.13	3.00e-57	215.00
IUUC-Tni-120521	Tetraodon nigroviridis	82.60	0.00e+00	657.00
IUUC-Ttr-128997	Tursiops truncatus	34.64	1.00e-53	203.00
IUUC-Vpa-130154	Vicugna pacos	36.73	8.00e-64	237.00
IUUC-Xtr-132805	Xenopus tropicalis	55.26	4.00e-46	178.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence