iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Rno-104916

UUCD1 version

UUC-RaN-00883

Ensembl Protein ID

ENSRNOP00000009115.7

UniProt Accession

Q62671; F1LRS0; UBR5_RAT

Genbank Protein ID

CAA45756.1

Protein Name

E3 ubiquitin-protein ligase UBR5; 100 kDa protein; E3 ubiquitin-protein ligase, HECT domain-containing 1; HECT-type E3 ubiquitin transferase UBR5; Hyperplastic discs protein homolog

Genbank Nucleotide ID

X64411

Gene Name

Ubr5; Dd5; Edd; Edd1; Hyd

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000006816.7	ENSRNOT00000009115.7	ENSRNOP00000009115.7

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
RAID2
Protein-protein Interaction
HINT	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
CPLM	dbPAF	UniProt
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	16554297
E3 activity/N-recognin/UBR-box	UBR	16554297

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	6.20e-80	269.5	2107	2429

Active Site

Position(s)	Description	Evidence
2757	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 17   egldlgGvtreflallskelqnpd..lglf.lysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlplsksfykll 100
           e+ + +G++r ++++ ++++ + d  ++lf +  +++ +t +p +++      +l++F+ +Gr+lg++l +++l++++l+++++k l
   Q: 2107 ENRKRHGSSRSVVDMDLDDTDDGDdnAPLFyQPGKRGFYTPRPGKNTE----ARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVL 2189
           556778**************9999778888334444777777777775....89********************************* PP
   S: 101  lgkklsledleevdpelyks.lkeikena..esdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvveyrlke 184
           lg+k++++d++ +dp +y+s  + i++++  ++d   +++dl+f v+ ++ +   ++eL+p+G nipVt +N +eYv++++e+r+  
   Q: 2190 LGRKVNWHDFAFFDPVMYESlRQLILASQssDADAVFSAMDLAFAVDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLV 2276
           ********************5556666655556888999************************************************ PP
   S: 185  gikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspai....qwFwevleefsseerkafLqF 267
             ++ ++a+r+G+ +v+p+++l+ +t+e+++ll++G+ e++v++l ++t+++++  e+ +++    +wFw+++e++s++er++++ F
   Q: 2277 VAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLlqfkRWFWSIVERMSMTERQDLVYF 2363
           ***************************************************9999999999989999******************** PP
   S: 268  vTgssrlPl..gGfaelkprlfvirkdasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           +T+s++lP+  +Gf++++  +++ir   +d++lP++nTC+++L +P Yssk+ilk+kLl+Ai++ k+F++
   Q: 2364 WTSSPSLPAseEGFQPMP--SITIRP-PDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKT-KNFGF 2429
           *********999******..799999.99**********************************9.99875 PP

Organism

Rattus norvegicus

Functional Description
(View)

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity).

Protein Sequence
(Fasta)

DGTKFTCIGA LYSELVAVSS KGELYQWKWT ESEPYRNAQN PSLHHPRATF LGLTNEKIVL 60
LSANSIRATV ATENNKVATW VDETLSSVAS KLEHTAQTYS ELQGERIVSL HCCALYTCAQ 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GATGGAACCA AGTTTACCTG CATTGGGGCT CTGTATTCTG AGCTTGTGGC GGTCAGCAGT 60
AAAGGAGAGC TTTACCAATG GAAGTGGACT GAGTCTGAGC CTTACAGAAA TGCCCAGGTA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR024725--E3_UbLigase_EDD_UBA
IPR000569--HECT_dom
IPR002004--PABP_HYD
IPR009091--RCC1/BLIP-II
IPR003126--Znf_UBR

PROSITE

PS50237--HECT
PS51309--PABC
PS51157--ZF_UBR

Pfam

PF11547--E3_UbLigase_EDD
PF00632--HECT
PF00658--PABP

SMART

SM00119--HECTc
SM00517--PolyA
SM00396--ZnF_UBR1

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0019904--F:protein domain specific binding
GO:0003723--F:RNA binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0008270--F:zinc ion binding
GO:0006974--P:cellular response to DNA damage stimulus
GO:0006281--P:DNA repair
GO:2000780--P:negative regulation of double-strand break repair
GO:1901315--P:negative regulation of histone H2A K63-linked ubiquitination
GO:0016567--P:protein ubiquitination

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002217	Ailuropoda melanoleuca	96.51	0.00e+00	3939.00
IUUC-Apl-004165	Anas platyrhynchos	94.46	0.00e+00	3882.00
IUUC-Aca-004577	Anolis carolinensis	93.28	0.00e+00	3784.00
IUUC-Ame-010702	Astyanax mexicanus	84.68	0.00e+00	2487.00
IUUC-Bta-012313	Bos taurus	96.55	0.00e+00	3985.00
IUUC-Cel-018165	Caenorhabditis elegans	35.31	8.00e-69	257.00
IUUC-Cja-019858	Callithrix jacchus	96.47	0.00e+00	4005.00
IUUC-Cfa-020973	Canis familiaris	96.51	0.00e+00	3985.00
IUUC-Cpo-022108	Cavia porcellus	93.27	0.00e+00	1825.00
IUUC-Csa-023985	Chlorocebus sabaeus	96.63	0.00e+00	4013.00
IUUC-Cho-025034	Choloepus hoffmanni	89.22	0.00e+00	987.00
IUUC-Cin-025715	Ciona intestinalis	45.21	0.00e+00	795.00
IUUC-Csv-026088	Ciona savignyi	38.33	4.00e-150	525.00
IUUC-Dre-027730	Danio rerio	87.15	0.00e+00	3462.00
IUUC-Dno-029123	Dasypus novemcinctus	93.73	0.00e+00	3824.00
IUUC-Dor-030757	Dipodomys ordii	91.06	0.00e+00	2310.00
IUUC-Dme-032192	Drosophila melanogaster	51.52	1.00e-173	605.00
IUUC-Ete-032292	Echinops telfairi	95.11	0.00e+00	2172.00
IUUC-Eca-034347	Equus caballus	96.26	0.00e+00	3922.00
IUUC-Eeu-034873	Erinaceus europaeus	91.49	0.00e+00	3765.00
IUUC-Fca-036485	Felis catus	94.87	0.00e+00	3855.00
IUUC-Fal-037071	Ficedula albicollis	95.36	0.00e+00	3892.00
IUUC-Gmo-039589	Gadus morhua	86.98	0.00e+00	3456.00
IUUC-Gga-041195	Gallus gallus	93.55	0.00e+00	3787.00
IUUC-Gac-042538	Gasterosteus aculeatus	86.56	0.00e+00	3497.00
IUUC-Ggo-045351	Gorilla gorilla	95.49	0.00e+00	4033.00
IUUC-Hsa-045941	Homo sapiens	96.47	0.00e+00	4027.00
IUUC-Itr-048635	Ictidomys tridecemlineatus	96.59	0.00e+00	3992.00
IUUC-Lch-050480	Latimeria chalumnae	92.24	0.00e+00	3801.00
IUUC-Loc-052664	Lepisosteus oculatus	87.72	0.00e+00	3588.00
IUUC-Laf-053935	Loxodonta africana	93.62	0.00e+00	1493.00
IUUC-Mcc-054703	Macaca mulatta	96.63	0.00e+00	4015.00
IUUC-Meu-056431	Macropus eugenii	87.32	0.00e+00	796.00
IUUC-Mga-059289	Meleagris gallopavo	95.21	0.00e+00	3521.00
IUUC-Mmr-061737	Microcebus murinus	96.18	0.00e+00	3918.00
IUUC-Mdo-062499	Monodelphis domestica	95.15	0.00e+00	3905.00
IUUC-Mmu-063273	Mus musculus	97.00	0.00e+00	3991.00
IUUC-Mpu-066907	Mustela putorius furo	96.51	0.00e+00	3938.00
IUUC-Mlu-067942	Myotis lucifugus	96.19	0.00e+00	3960.00
IUUC-Nle-069527	Nomascus leucogenys	96.55	0.00e+00	4036.00
IUUC-Opr-070626	Ochotona princeps	94.65	0.00e+00	1285.00
IUUC-Ont-071951	Oreochromis niloticus	86.75	0.00e+00	3456.00
IUUC-Oan-072958	Ornithorhynchus anatinus	95.73	0.00e+00	3900.00
IUUC-Ocu-074503	Oryctolagus cuniculus	94.71	0.00e+00	3914.00
IUUC-Ola-086757	Oryzias latipes	86.86	0.00e+00	3528.00
IUUC-Oga-088781	Otolemur garnettii	96.38	0.00e+00	3974.00
IUUC-Oar-089274	Ovis aries	96.55	0.00e+00	3947.00
IUUC-Ptr-090669	Pan troglodytes	96.34	0.00e+00	3908.00
IUUC-Pan-092226	Papio anubis	96.35	0.00e+00	3986.00
IUUC-Psi-093502	Pelodiscus sinensis	95.28	0.00e+00	3892.00
IUUC-Pma-094454	Petromyzon marinus	77.31	0.00e+00	735.00
IUUC-Pfo-096173	Poecilia formosa	86.46	0.00e+00	3502.00
IUUC-Pab-098355	Pongo abelii	96.07	0.00e+00	2816.00
IUUC-Pca-100570	Procavia capensis	98.06	0.00e+00	1169.00
IUUC-Pva-102572	Pteropus vampyrus	94.35	0.00e+00	1356.00
IUUC-Sha-106653	Sarcophilus harrisii	95.36	0.00e+00	3917.00
IUUC-Sar-113517	Sorex araneus	97.23	0.00e+00	1117.00
IUUC-Ssc-115470	Sus scrofa	98.42	0.00e+00	2518.00
IUUC-Tgu-116932	Taeniopygia guttata	95.11	0.00e+00	3878.00
IUUC-Tru-117682	Takifugu rubripes	87.96	0.00e+00	2578.00
IUUC-Tsy-119417	Tarsius syrichta	94.14	0.00e+00	2467.00
IUUC-Tni-120442	Tetraodon nigroviridis	82.24	0.00e+00	3160.00
IUUC-Tbe-127456	Tupaia belangeri	84.21	0.00e+00	2054.00
IUUC-Ttr-128606	Tursiops truncatus	98.50	0.00e+00	1028.00
IUUC-Vpa-130112	Vicugna pacos	93.76	0.00e+00	3610.00
IUUC-Xtr-131929	Xenopus tropicalis	91.79	0.00e+00	3809.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence