iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046157

UUCD1 version

UUC-HoS-01064

Ensembl Protein ID

ENSP00000396901.2

UniProt Accession

P61077; A6NJ93; A6NJB1; A6NM99; P47986; Q6IB88; Q6NXS4; Q8N924; UB2D3_HUMAN

Genbank Protein ID

AAA91461.1; AAF35234.1; BAC04632.1; CAG33197.1; EAX06138.1; EAX06143.1; AAH03395.1; AAH37894.1; AAH66917.1

Protein Name

Ubiquitin-conjugating enzyme E2 D3; (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D3; Ubiquitin carrier protein D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; Ubiquitin-protein ligase D3

Genbank Nucleotide ID

U39318; AF213884; AK095822; DB045280; AC018797; CH471057; BC003395; BC037894; BC066917

Gene Name

UBE2D3; UBC5C; UBCH5C

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000109332.19	ENST00000453744.6	ENSP00000396901.2
ENSG00000109332.19	ENST00000343106.9	ENSP00000345285.5
ENSG00000109332.19	ENST00000350435.11	ENSP00000337262.7
ENSG00000109332.19	ENST00000357194.10	ENSP00000349722.6
ENSG00000109332.19	ENST00000505307.5	ENSP00000422939.1
ENSG00000109332.19	ENST00000510129.5	ENSP00000422134.1
ENSG00000109332.19	ENST00000508974.5	ENSP00000423324.1
ENSG00000109332.19	ENST00000618836.4	ENSP00000481600.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	TargetScan	RepTar	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	PHOSIDA	BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E2/UBC	E2_UBC	18784257; 18948756; 20145714; 16243356; 17786241; 18552202; 17604280; 16522193; 14707120; 19690564; 14684739
UBD/UBC-like/UBD_UBC	UBD_UBC	16543155

Classification

Family	E-value	Score	Start	End
E2/UBC	2.40e-55	187.8	5	138
UBD/UBC-like/UBD_UBC	1.10e-84	283.1	5	142

Active Site

Position(s)	Description	Evidence
85	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 1    RlkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilkeeekW 92
           R++kel++l++dpp+++sa+pv + d+++w+++i+Gp+d+pY+ggvF l+i+fp+dYPfkPPkv f+t+i+hPn+++nG++Cl+il+  ++W
   Q: 5    RINKELSDLARDPPAQCSAGPVGD-DMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILR--SQW 93
           89**********************.9************************************************************9..*** PP
   S: 93   spalsvesvllsiqsllaepnpesplneeaaellkknreeykkkv 137
           spal++++vllsi+sll++pnp++pl  e+a+++k++r +y+++ 
   Q: 94   SPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRIS 138
           *****************************************9876 PP

UBD/UBC-like/UBD_UBC

   S: 1    rikkelkdllrdeeaqisadlvdddlfelratiagppdspyeggvffltiklptdypfkppkvafitkiyhpninsngsicldilrsqwsaa 92
           ri+kel+dl+rd++aq+sa++v+dd+f+++ati+gp+dspy+ggvfflti++ptdypfkppkvaf+t+iyhpninsngsicldilrsqws+a
   Q: 5    RINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPA 96
           8******************************************************************************************* PP
   S: 93   ltlskvllslcslladaepddplvaeiariyktdkekykriarewt 138
           lt+skvlls+csll+d++pddplv+eiariyktd++ky+ri+rewt
   Q: 97   LTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWT 142
           *********************************************8 PP

Organism

Homo sapiens

Functional Description
(View)

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.

Protein Sequence
(Fasta)

MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY 60
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CGCGGCGGAC TGCCGAGGCG CGGGAACTGG CGGGTAGCGA GGCCCTCCTC GGAATCTCGT 60
GTGAAGGTGG CCCTCCTCTT GGGCCTTTAA CGTCTGTAGA TGCTGGAGAC CAGCAGAAAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0053--Apoptosis
KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-1015--Disulfide bond
KW-0227--DNA damage
KW-0234--DNA repair
KW-0967--Endosome
KW-0472--Membrane
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005829--C:cytosol
GO:0010008--C:endosome membrane
GO:0070062--C:extracellular exosome
GO:0005654--C:nucleoplasm
GO:0005886--C:plasma membrane
GO:0005524--F:ATP binding
GO:0061631--F:ubiquitin conjugating enzyme activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006915--P:apoptotic process
GO:0030509--P:BMP signaling pathway
GO:0006464--P:cellular protein modification process
GO:0006281--P:DNA repair
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:1903955--P:positive regulation of protein targeting to mitochondrion
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865--P:protein autoubiquitination
GO:0070979--P:protein K11-linked ubiquitination
GO:0070936--P:protein K48-linked ubiquitination
GO:0006513--P:protein monoubiquitination
GO:0000209--P:protein polyubiquitination
GO:0016567--P:protein ubiquitination
GO:0061418--P:regulation of transcription from RNA polymerase II promoter in response to hypoxia
GO:0035666--P:TRIF-dependent toll-like receptor signaling pathway
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:7323

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Apl-004043	Anas platyrhynchos	94.56	6.00e-82	295.00
IUUC-Aor-010157	Aspergillus oryzae	85.03	2.00e-74	270.00
IUUC-Cpo-022334	Cavia porcellus	97.96	3.00e-83	300.00
IUUC-Cin-025379	Ciona intestinalis	86.30	1.00e-76	278.00
IUUC-Dno-029156	Dasypus novemcinctus	98.60	3.00e-82	296.00
IUUC-Dor-030285	Dipodomys ordii	100.00	8.00e-81	291.00
IUUC-Eca-034286	Equus caballus	100.00	8.00e-81	291.00
IUUC-Eeu-034766	Erinaceus europaeus	97.14	1.00e-78	284.00
IUUC-Fca-035917	Felis catus	100.00	1.00e-85	307.00
IUUC-Fal-036904	Ficedula albicollis	100.00	2.00e-81	293.00
IUUC-Fso-038297	Fusarium solani	83.67	1.00e-73	268.00
IUUC-Gmo-038898	Gadus morhua	97.90	4.00e-82	296.00
IUUC-Gga-041015	Gallus gallus	97.96	1.00e-84	305.00
IUUC-Ggo-045076	Gorilla gorilla	96.53	1.00e-80	291.00
IUUC-Meu-056462	Macropus eugenii	98.56	1.00e-79	288.00
IUUC-Mla-059189	Melampsora laricipopulina	80.95	8.00e-69	252.00
IUUC-Mga-059237	Meleagris gallopavo	100.00	2.00e-81	293.00
IUUC-Mmr-060889	Microcebus murinus	97.96	1.00e-84	305.00
IUUC-Mdo-062596	Monodelphis domestica	99.32	2.00e-85	306.00
IUUC-Mmu-063796	Mus musculus	97.96	1.00e-84	305.00
IUUC-Ncr-068677	Neurospora crassa	82.99	2.00e-72	264.00
IUUC-Opr-070191	Ochotona princeps	100.00	8.00e-81	291.00
IUUC-Oga-088477	Otolemur garnettii	95.21	2.00e-82	296.00
IUUC-Oar-090038	Ovis aries	100.00	1.00e-85	307.00
IUUC-Ptr-091438	Pan troglodytes	98.64	1.00e-84	304.00
IUUC-Psi-094076	Pelodiscus sinensis	100.00	1.00e-85	307.00
IUUC-Pab-098293	Pongo abelii	98.66	3.00e-84	303.00
IUUC-Rno-105498	Rattus norvegicus	100.00	1.00e-85	307.00
IUUC-Spo-108032	Schizosaccharomyces pombe	84.35	1.00e-73	268.00
IUUC-Sre-115037	Sporisorium reilianum	81.12	1.00e-69	254.00
IUUC-Ssc-116154	Sus scrofa	95.92	1.00e-82	297.00
IUUC-Tgu-116727	Taeniopygia guttata	100.00	1.00e-85	307.00
IUUC-Tme-126912	Tuber melanosporum	82.31	4.00e-72	263.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence