iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	43.56	1.00e-17	87.00	IUUC-Ath-006802
E2/Autophagy_C	45.00	6.00e-18	89.00	IUUC-Hsa-046449
E2/Autophagy_C	30.77	7.00e-17	82.40	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
227	Glycyl thioester intermediate	{ECO:0000250}

Organism

Schizosaccharomyces pombe

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity). Plays a role in meiosis and sporulation.

Protein Sequence
(Fasta)

MAQRLTSAFL NWREHITPAS KTSDFENTGM ISPEEFVLAG DYLVSKFPTW SWECGDRIRG 60
FLPKDKQYLV TRHVFCVQRN INIGVNEEWV DIETDDTRNK DDDQDDDAIS SIHSDTSDIA 120

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Nucleotide Sequence
(Fasta)

CGCTAATTAA CACTGATCTC CGTACTGCCC GGTTTTGATC ATTGCTACTG AGTAATTATT 60
GTAGTTATTC TTTTAAAAAT TGCAATTTGA AATGTGATAC TTGTTTCTTA TAAGCTTTCC 120

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Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0539--Nucleus
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006914--P:autophagy
GO:0006501--P:C-terminal protein lipidation
GO:0016236--P:macroautophagy
GO:0000422--P:mitophagy
GO:0044804--P:nucleophagy
GO:0015031--P:protein transport

KEGG

spo:SPBC3B9.06c

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	32.66	4.00e-33	133.00
IUUC-Aml-002081	Ailuropoda melanoleuca	36.24	2.00e-43	167.00
IUUC-Atr-002712	Amborella trichopoda	33.45	8.00e-35	138.00
IUUC-Apl-003956	Anas platyrhynchos	32.37	2.00e-25	107.00
IUUC-Aca-004578	Anolis carolinensis	37.25	6.00e-45	172.00
IUUC-Aly-005823	Arabidopsis lyrata	36.51	5.00e-46	176.00
IUUC-Ath-006802	Arabidopsis thaliana	36.51	6.00e-46	175.00
IUUC-Ago-007855	Ashbya gossypii	33.12	3.00e-44	169.00
IUUC-Acl-008147	Aspergillus clavatus	45.00	1.00e-16	78.60
IUUC-Afl-008483	Aspergillus flavus	54.43	2.00e-19	87.40
IUUC-Afu-008818	Aspergillus fumigatus	40.78	3.00e-35	140.00
IUUC-Ani-009481	Aspergillus nidulans	53.75	1.00e-18	85.10
IUUC-Ang-009800	Aspergillus niger	35.81	4.00e-22	96.70
IUUC-Aor-010150	Aspergillus oryzae	54.43	2.00e-19	87.40
IUUC-Ate-010545	Aspergillus terreus	47.30	3.00e-17	79.70
IUUC-Ame-011793	Astyanax mexicanus	36.63	2.00e-44	170.00
IUUC-Bgr-012167	Blumeria graminis	37.34	1.00e-33	135.00
IUUC-Bta-012695	Bos taurus	36.24	8.00e-44	168.00
IUUC-Bci-013972	Botrytis cinerea	52.05	1.00e-15	75.10
IUUC-Bdi-014756	Brachypodium distachyon	35.16	8.00e-42	161.00
IUUC-Bol-015091	Brassica oleracea	34.19	6.00e-42	162.00
IUUC-Bra-016662	Brassica rapa	42.75	5.00e-25	105.00
IUUC-Cel-018143	Caenorhabditis elegans	34.75	4.00e-40	156.00
IUUC-Cja-018991	Callithrix jacchus	37.12	7.00e-45	172.00
IUUC-Cfa-020070	Canis familiaris	36.24	8.00e-44	168.00
IUUC-Cpo-021860	Cavia porcellus	36.24	1.00e-43	167.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	34.44	1.00e-42	164.00
IUUC-Csa-023881	Chlorocebus sabaeus	36.24	3.00e-43	166.00
IUUC-Cho-024852	Choloepus hoffmanni	49.56	5.00e-27	112.00
IUUC-Cin-025787	Ciona intestinalis	37.00	3.00e-48	183.00
IUUC-Csv-026369	Ciona savignyi	36.24	9.00e-47	178.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	38.06	1.00e-36	145.00
IUUC-Cne-026905	Cryptococcus neoformans	48.28	1.00e-18	85.10
IUUC-Dre-028811	Danio rerio	36.54	1.00e-43	168.00
IUUC-Dno-030081	Dasypus novemcinctus	36.67	8.00e-43	165.00
IUUC-Dor-030183	Dipodomys ordii	44.44	7.00e-17	79.30
IUUC-Dse-031539	Dothistroma septosporum	44.44	1.00e-09	55.10
IUUC-Dme-031805	Drosophila melanogaster	50.44	2.00e-28	117.00
IUUC-Ete-033171	Echinops telfairi	37.04	1.00e-44	171.00
IUUC-Eca-034083	Equus caballus	36.24	1.00e-43	168.00
IUUC-Eeu-034804	Erinaceus europaeus	36.24	8.00e-42	161.00
IUUC-Fca-036289	Felis catus	36.91	1.00e-43	168.00
IUUC-Fal-037060	Ficedula albicollis	35.91	2.00e-44	170.00
IUUC-Fox-038051	Fusarium oxysporum	34.81	1.00e-37	148.00
IUUC-Fso-038124	Fusarium solani	55.71	1.00e-16	78.60
IUUC-Gmo-039538	Gadus morhua	37.12	1.00e-42	164.00
IUUC-Ggr-039880	Gaeumannomyces graminis	33.99	4.00e-38	149.00
IUUC-Gga-041108	Gallus gallus	38.38	6.00e-45	172.00
IUUC-Gac-041558	Gasterosteus aculeatus	37.85	2.00e-44	170.00
IUUC-Gma-043969	Glycine max	34.41	5.00e-42	162.00
IUUC-Ggo-045071	Gorilla gorilla	36.24	3.00e-43	166.00
IUUC-Hsa-046449	Homo sapiens	36.24	3.00e-43	166.00
IUUC-Hvu-047451	Hordeum vulgare	32.91	9.00e-40	155.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	36.24	3.00e-43	166.00
IUUC-Kpa-049096	Komagataella pastoris	33.95	6.00e-42	162.00
IUUC-Lch-049726	Latimeria chalumnae	37.92	2.00e-46	177.00
IUUC-Lpe-050734	Leersia perrieri	34.04	1.00e-34	138.00
IUUC-Loc-051983	Lepisosteus oculatus	37.79	4.00e-45	172.00
IUUC-Lma-053038	Leptosphaeria maculans	29.41	4.00e-34	136.00
IUUC-Laf-054510	Loxodonta africana	36.27	7.00e-41	158.00
IUUC-Mcc-054792	Macaca mulatta	36.24	3.00e-43	166.00
IUUC-Meu-055965	Macropus eugenii	33.06	4.00e-25	106.00
IUUC-Mor-057292	Magnaporthe oryzae	39.37	5.00e-35	139.00
IUUC-Mtr-058282	Medicago truncatula	34.09	1.00e-42	164.00
IUUC-Mla-059144	Melampsora laricipopulina	52.50	2.00e-20	91.30
IUUC-Mga-059509	Meleagris gallopavo	37.92	2.00e-44	171.00
IUUC-Mvi-060281	Microbotryum violaceum	36.75	2.00e-23	101.00
IUUC-Mmr-061788	Microcebus murinus	36.58	2.00e-44	170.00
IUUC-Mdo-062922	Monodelphis domestica	35.91	2.00e-43	167.00
IUUC-Mmu-064249	Mus musculus	36.58	2.00e-44	170.00
IUUC-Mac-064474	Musa acuminata	34.26	4.00e-39	152.00
IUUC-Mpu-065717	Mustela putorius furo	36.24	2.00e-43	167.00
IUUC-Mlu-067151	Myotis lucifugus	34.01	1.00e-37	147.00
IUUC-Nfi-068551	Neosartorya fischeri	53.16	9.00e-19	85.50
IUUC-Ncr-068846	Neurospora crassa	60.61	1.00e-17	81.60
IUUC-Nle-069790	Nomascus leucogenys	36.24	3.00e-43	166.00
IUUC-Ont-072517	Oreochromis niloticus	35.69	9.00e-43	164.00
IUUC-Oan-073285	Ornithorhynchus anatinus	49.56	5.00e-27	112.00
IUUC-Ocu-074695	Oryctolagus cuniculus	36.24	3.00e-43	166.00
IUUC-Oba-074991	Oryza barthii	36.54	4.00e-43	166.00
IUUC-Obr-076787	Oryza brachyantha	34.08	3.00e-41	160.00
IUUC-Ogl-077440	Oryza glaberrima	34.29	4.00e-42	162.00
IUUC-Ogu-078294	Oryza glumaepatula	34.85	1.00e-42	164.00
IUUC-Oin-079452	Oryza indica	34.39	3.00e-42	163.00
IUUC-Olo-080450	Oryza longistaminata	36.54	1.00e-43	168.00
IUUC-Ome-081514	Oryza meridionalis	36.13	1.00e-42	164.00
IUUC-Oni-082677	Oryza nivara	34.39	3.00e-42	163.00
IUUC-Opu-083925	Oryza punctata	33.97	3.00e-41	160.00
IUUC-Oru-084828	Oryza rufipogon	35.79	7.00e-44	169.00
IUUC-Osa-085937	Oryza sativa	34.08	1.00e-41	161.00
IUUC-Ola-086559	Oryzias latipes	31.60	1.00e-21	94.40
IUUC-Olu-087766	Ostreococcus lucimarinus	35.15	1.00e-41	161.00
IUUC-Oga-087983	Otolemur garnettii	36.12	7.00e-43	165.00
IUUC-Oar-089281	Ovis aries	36.24	8.00e-44	168.00
IUUC-Ptr-090983	Pan troglodytes	36.24	3.00e-43	166.00
IUUC-Pan-092457	Papio anubis	36.24	3.00e-43	166.00
IUUC-Psi-094046	Pelodiscus sinensis	37.32	1.00e-41	160.00
IUUC-Pno-094925	Phaeosphaeria nodorum	33.14	3.00e-20	90.50
IUUC-Ppa-095487	Physcomitrella patens	35.76	7.00e-43	165.00
IUUC-Pfo-096968	Poecilia formosa	36.96	7.00e-45	172.00
IUUC-Pab-098621	Pongo abelii	36.58	4.00e-42	162.00
IUUC-Pop-100007	Populus trichocarpa	34.82	3.00e-42	163.00
IUUC-Pca-100590	Procavia capensis	48.70	4.00e-27	112.00
IUUC-Ppe-101657	Prunus persica	36.45	8.00e-44	168.00
IUUC-Pva-102446	Pteropus vampyrus	36.24	3.00e-44	169.00
IUUC-Pgr-103604	Puccinia graminis	51.25	4.00e-20	90.10
IUUC-Ptt-103941	Puccinia triticina	51.25	3.00e-20	90.50
IUUC-Pte-104252	Pyrenophora teres	32.56	2.00e-20	91.30
IUUC-Pyt-104556	Pyrenophora triticirepentis	32.56	2.00e-20	91.30
IUUC-Rno-106025	Rattus norvegicus	36.91	2.00e-43	167.00
IUUC-Sce-106172	Saccharomyces cerevisiae	32.70	2.00e-39	154.00
IUUC-Sha-106858	Sarcophilus harrisii	37.37	1.00e-43	167.00
IUUC-Sja-107905	Schizosaccharomyces japonicus	60.28	2.00e-94	336.00
IUUC-Smo-108883	Selaginella moellendorffii	33.76	3.00e-41	159.00
IUUC-Sit-110512	Setaria italica	34.74	2.00e-40	157.00
IUUC-Sly-111238	Solanum lycopersicum	36.25	2.00e-42	164.00
IUUC-Stu-112441	Solanum tuberosum	36.25	2.00e-42	163.00
IUUC-Sar-113039	Sorex araneus	48.70	4.00e-27	112.00
IUUC-Sbi-113776	Sorghum bicolor	34.41	5.00e-41	159.00
IUUC-Sre-115074	Sporisorium reilianum	46.24	3.00e-17	80.90
IUUC-Ssc-115262	Sus scrofa	36.24	1.00e-43	167.00
IUUC-Tgu-116790	Taeniopygia guttata	37.58	6.00e-44	169.00
IUUC-Tru-118160	Takifugu rubripes	35.39	7.00e-44	168.00
IUUC-Tsy-119145	Tarsius syrichta	33.63	9.00e-12	62.00
IUUC-Tni-119937	Tetraodon nigroviridis	34.10	2.00e-37	147.00
IUUC-Tca-121323	Theobroma cacao	34.94	4.00e-42	162.00
IUUC-Tre-121993	Trichoderma reesei	40.64	4.00e-37	146.00
IUUC-Tvi-122332	Trichoderma virens	40.24	1.00e-37	148.00
IUUC-Tae-124391	Triticum aestivum	33.65	2.00e-40	157.00
IUUC-Tur-126679	Triticum urartu	33.54	3.00e-40	156.00
IUUC-Tme-127159	Tuber melanosporum	46.75	1.00e-11	61.20
IUUC-Tbe-127590	Tupaia belangeri	27.41	2.00e-05	40.40
IUUC-Ttr-128261	Tursiops truncatus	36.14	5.00e-41	159.00
IUUC-Uma-129572	Ustilago maydis	47.31	1.00e-18	85.50
IUUC-Vda-129966	Verticillium dahliae	39.29	2.00e-36	144.00
IUUC-Vpa-130698	Vicugna pacos	49.56	4.00e-27	112.00
IUUC-Xtr-132878	Xenopus tropicalis	37.12	5.00e-46	176.00
IUUC-Xma-134017	Xiphophorus maculatus	36.30	2.00e-44	170.00
IUUC-Yli-134373	Yarrowia lipolytica	34.09	3.00e-41	160.00
IUUC-Zma-135222	Zea mays	45.61	1.00e-24	104.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence