iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Tae-125950

Ensembl Protein ID

TRIAE_CS42_5AL_TGACv1_375074_AA1215540.1

UniProt Accession

A0A1D5YHG2; A0A1D5YHG2_WHEAT

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
TRIAE_CS42_5AL_TGACv1_375074_AA1215540	TRIAE_CS42_5AL_TGACv1_375074_AA1215540.1	TRIAE_CS42_5AL_TGACv1_375074_AA1215540.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	1.50e-103	349.9	20	526

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedv 69
           YDRQ+r+wG+++q++l++a+++l+++g++G+E lKnLvl+G+gs+tvvD+++ve s+l+++FL+++e  
   Q: 20   YDRQLRIWGDQGQAALEKASICLLNCGPTGTEALKNLVLGGIGSVTVVDGSKVEPSDLGNNFLLDEECL 88
           ********************************************************************* PP
   S: 70   gksKAevaaealkelnpdvnveaheesiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpl 137
           g+s+A++ + +l+eln+ v+++ +ees+ +  ++++ Ff++f+vV++++ ++++ +k++++c +a++ l
   Q: 89   GHSRAKSICSFLQELNDAVKAKYVEESPATMIDTNPsFFSQFTVVIATQLPESSLLKLDSICGSANIVL 157
           **********************************99********************************* PP
   S: 138  iesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn.........aaehtiewavlfn 194
           + ++++Gl G vrv+ik++ +++s++d   d++++ +P+ +lk++           +++++++++v++ 
   Q: 158  VAARSYGLTGLVRVSIKEHCVIESKPDhflDDLRLHNPWTELKQFAKsidicdkdaVVHKHTPYIVILV 226
           *********************************************88788888888************* PP
   S: 195  klleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfec 262
            l+e++a +++++ +++++ek+e+kd++++ + + de+n++ea+e + k   +  i++ i+q++++ + 
   Q: 227  GLAEKWADAHDGQLPSTRQEKREFKDLIRAHMLNVDEDNYKEAVESSYKVSVTPGISNeIRQIIDDNSS 295
           ******************************************************************555 PP
   S: 263  divtkskspfwv......................................................... 274
            +v++s+s+fwv                                                         
   Q: 296  -EVNSSSSDFWVlvsalkefipnegngelplegtipdmtslteyyvslqkiyqakaesdclamehrvks 363
           .89****************************************************************** PP
   S: 275  ..................sfcsnaealq...........vpekvekdeevvkasap.....lylllral 309
                             +fc+n+++l+           + +   + +++++++++     +y+llra+
   Q: 364  ilkrigrdpesisrayikTFCKNTRKLKvcryrsmeeefSSPVLSEVQKYFADEDScyamnFYVLLRAV 432
           ****************************666666555542233333344444444466888******** PP
   S: 310  erfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattnavvg 377
           +r+++ ++r pg ++ e  +d +++l++aa +   +++g+++  l++dl++e+++++ ++i+++ a++g
   Q: 433  DRLAASYSRLPGIFDSEIVED-VPRLKAAAVSVL-SDMGLKGaSLSEDLVTEVCRFAGAEIHPVGAFIG 499
           ************999999999.999999998877.********************************** PP
   S: 378  GlaaqEviKvvsgkfeplknfflfdae 404
           G+a+qEviK+v+++f+pl+ +f+f+++
   Q: 500  GVASQEVIKLVTKQFVPLNGTFIFNGI 526
           ************************986 PP

Organism

Triticum aestivum

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MASAAMAAAA VATAEPKTKY DRQLRIWGDQ GQAALEKASI CLLNCGPTGT EALKNLVLGG 60
IGSVTVVDGS KVEPSDLGNN FLLDEECLGH SRAKSICSFL QELNDAVKAK YVEESPATMI 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CTGGCTCCGC ACTTCCACCT CCGCTCCTCG CTTTATTCCA CGGCGGCGCC GGAGAGGAGG 60
CGGCGAGGCG AGGCGAGGCG GAAACCACCA CACCCACACA GTGAAGAGAC TCCGCCTCCG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ame-011183	Astyanax mexicanus	41.63	6.00e-123	434.00
IUUC-Bdi-014592	Brachypodium distachyon	91.94	0.00e+00	969.00
IUUC-Dre-028140	Danio rerio	41.39	2.00e-124	440.00
IUUC-Kpa-049309	Komagataella pastoris	35.58	6.00e-76	278.00
IUUC-Lch-050038	Latimeria chalumnae	43.07	4.00e-124	438.00
IUUC-Mac-064521	Musa acuminata	73.03	0.00e+00	798.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence