iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Afu-008818

Ensembl Protein ID

CADAFUAP00006382

UniProt Accession

Q4WUE5; ATG3_ASPFU

Genbank Protein ID

EAL91781.1

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3

Genbank Nucleotide ID

AAHF01000003

Gene Name

AFUA_5G08170; atg3

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
CADAFUAG00006382	CADAFUAT00006382	CADAFUAP00006382

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	32.37	2.00e-36	150.00	IUUC-Ath-006802
E2/Autophagy_C	31.94	2.00e-35	147.00	IUUC-Hsa-046449
E2/Autophagy_C	37.12	4.00e-56	213.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
245	Glycyl thioester intermediate	{ECO:0000250}

Organism

Aspergillus fumigatus

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNILHSTLST WRDRLAPVSR TSTFRTTGQI TPEEFVLAGD YLVYKFPTWS WADASSPAKR 60
VSYLPPGKQF LVTRGVPCHR RLNENFAGDA GHEDEIVRDM LSGADADDDD GWLRTGGGRD 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGAACATCC TCCACTCCAC CTTATCAACC TGGCGGGATC GCCTGGCCCC TGTCTCCCGC 60
ACCTCCACCT TCCGCACCAC CGGCCAAATC ACCCCAGAGG AGTTCGTGTT GGCAGGCGAC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

KEGG

afm:AFUA_5G08170

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	35.94	2.00e-33	134.00
IUUC-Aml-002081	Ailuropoda melanoleuca	33.15	3.00e-20	90.50
IUUC-Atr-002712	Amborella trichopoda	34.78	1.00e-28	118.00
IUUC-Apl-003956	Anas platyrhynchos	50.00	5.00e-12	63.20
IUUC-Aca-004578	Anolis carolinensis	32.97	3.00e-20	90.50
IUUC-Aly-005823	Arabidopsis lyrata	31.91	4.00e-39	153.00
IUUC-Ath-006802	Arabidopsis thaliana	32.37	3.00e-38	150.00
IUUC-Ago-007855	Ashbya gossypii	36.90	1.00e-53	201.00
IUUC-Acl-008147	Aspergillus clavatus	81.99	1.00e-151	526.00
IUUC-Afl-008483	Aspergillus flavus	83.47	4.00e-149	518.00
IUUC-Ani-009481	Aspergillus nidulans	77.24	4.00e-141	492.00
IUUC-Ang-009800	Aspergillus niger	73.95	1.00e-136	477.00
IUUC-Aor-010150	Aspergillus oryzae	83.47	4.00e-149	518.00
IUUC-Ate-010545	Aspergillus terreus	80.08	1.00e-84	304.00
IUUC-Ame-011793	Astyanax mexicanus	33.15	8.00e-21	92.40
IUUC-Bgr-012167	Blumeria graminis	63.04	4.00e-106	375.00
IUUC-Bta-012695	Bos taurus	32.97	3.00e-20	90.50
IUUC-Bci-013972	Botrytis cinerea	66.85	1.00e-97	348.00
IUUC-Bdi-014756	Brachypodium distachyon	32.32	3.00e-39	153.00
IUUC-Bol-015091	Brassica oleracea	31.98	9.00e-37	145.00
IUUC-Bra-016662	Brassica rapa	34.86	1.00e-21	95.10
IUUC-Cel-018143	Caenorhabditis elegans	51.19	2.00e-21	94.00
IUUC-Cja-018991	Callithrix jacchus	32.13	5.00e-39	152.00
IUUC-Cfa-020070	Canis familiaris	32.97	3.00e-20	90.50
IUUC-Cpo-021860	Cavia porcellus	32.97	3.00e-20	90.50
IUUC-Cre-022948	Chlamydomonas reinhardtii	33.33	1.00e-20	91.70
IUUC-Csa-023881	Chlorocebus sabaeus	32.97	3.00e-20	90.50
IUUC-Cho-024852	Choloepus hoffmanni	32.97	4.00e-20	90.10
IUUC-Cin-025787	Ciona intestinalis	35.69	8.00e-36	142.00
IUUC-Csv-026369	Ciona savignyi	38.40	3.00e-37	147.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	61.13	4.00e-99	352.00
IUUC-Cne-026905	Cryptococcus neoformans	36.17	6.00e-23	100.00
IUUC-Dre-028811	Danio rerio	33.15	2.00e-20	91.70
IUUC-Dno-030081	Dasypus novemcinctus	32.97	4.00e-20	90.10
IUUC-Dor-030183	Dipodomys ordii	54.17	6.00e-19	86.30
IUUC-Dse-031539	Dothistroma septosporum	52.05	7.00e-66	242.00
IUUC-Dme-031805	Drosophila melanogaster	50.00	3.00e-19	87.40
IUUC-Ete-033171	Echinops telfairi	34.07	2.00e-21	94.00
IUUC-Eca-034083	Equus caballus	32.97	3.00e-20	90.90
IUUC-Eeu-034804	Erinaceus europaeus	32.42	8.00e-20	89.00
IUUC-Fca-036289	Felis catus	32.97	3.00e-20	90.90
IUUC-Fal-037060	Ficedula albicollis	32.97	3.00e-20	90.90
IUUC-Fox-038051	Fusarium oxysporum	64.25	4.00e-106	376.00
IUUC-Fso-038124	Fusarium solani	64.71	9.00e-95	338.00
IUUC-Gmo-039538	Gadus morhua	33.89	1.00e-37	148.00
IUUC-Ggr-039880	Gaeumannomyces graminis	59.84	1.00e-109	387.00
IUUC-Gga-041108	Gallus gallus	32.97	3.00e-20	90.50
IUUC-Gac-041558	Gasterosteus aculeatus	32.05	2.00e-37	147.00
IUUC-Gma-043969	Glycine max	29.57	1.00e-37	148.00
IUUC-Ggo-045071	Gorilla gorilla	32.97	3.00e-20	90.90
IUUC-Hsa-046449	Homo sapiens	32.97	3.00e-20	90.90
IUUC-Hvu-047451	Hordeum vulgare	37.11	7.00e-33	132.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	32.97	3.00e-20	90.90
IUUC-Kpa-049096	Komagataella pastoris	39.71	5.00e-58	216.00
IUUC-Lch-049726	Latimeria chalumnae	31.45	9.00e-38	148.00
IUUC-Lpe-050734	Leersia perrieri	38.81	1.00e-38	151.00
IUUC-Loc-051983	Lepisosteus oculatus	33.91	4.00e-19	87.00
IUUC-Lma-053038	Leptosphaeria maculans	61.80	1.00e-109	387.00
IUUC-Laf-054510	Loxodonta africana	32.97	2.00e-20	90.90
IUUC-Mcc-054792	Macaca mulatta	32.97	3.00e-20	90.50
IUUC-Meu-055965	Macropus eugenii	52.50	1.00e-18	85.10
IUUC-Mor-057292	Magnaporthe oryzae	61.39	1.00e-102	364.00
IUUC-Mtr-058282	Medicago truncatula	30.98	4.00e-38	149.00
IUUC-Mla-059144	Melampsora laricipopulina	38.07	9.00e-27	112.00
IUUC-Mga-059509	Meleagris gallopavo	32.97	3.00e-20	90.50
IUUC-Mvi-060281	Microbotryum violaceum	40.22	5.00e-26	110.00
IUUC-Mmr-061788	Microcebus murinus	32.97	3.00e-20	90.90
IUUC-Mdo-062922	Monodelphis domestica	32.97	3.00e-20	90.90
IUUC-Mmu-064249	Mus musculus	32.97	3.00e-20	90.90
IUUC-Mac-064474	Musa acuminata	31.87	5.00e-37	145.00
IUUC-Mpu-065717	Mustela putorius furo	32.97	3.00e-20	90.90
IUUC-Mlu-067151	Myotis lucifugus	58.73	2.00e-17	81.30
IUUC-Nfi-068551	Neosartorya fischeri	98.02	0.00e+00	637.00
IUUC-Ncr-068846	Neurospora crassa	62.67	2.00e-104	370.00
IUUC-Nle-069790	Nomascus leucogenys	32.97	3.00e-20	90.90
IUUC-Opr-070828	Ochotona princeps	22.11	2.10e-01	27.30
IUUC-Ont-072517	Oreochromis niloticus	33.15	5.00e-21	93.20
IUUC-Oan-073285	Ornithorhynchus anatinus	33.91	5.00e-20	89.40
IUUC-Ocu-074695	Oryctolagus cuniculus	32.97	3.00e-20	90.50
IUUC-Oba-074991	Oryza barthii	32.78	5.00e-41	159.00
IUUC-Obr-076787	Oryza brachyantha	35.31	4.00e-40	156.00
IUUC-Ogl-077440	Oryza glaberrima	33.24	2.00e-40	157.00
IUUC-Ogu-078294	Oryza glumaepatula	34.46	6.00e-41	159.00
IUUC-Oin-079452	Oryza indica	34.18	6.00e-41	159.00
IUUC-Olo-080450	Oryza longistaminata	33.52	6.00e-42	162.00
IUUC-Ome-081514	Oryza meridionalis	34.65	1.00e-40	158.00
IUUC-Oni-082677	Oryza nivara	34.18	6.00e-41	159.00
IUUC-Opu-083925	Oryza punctata	34.75	4.00e-40	156.00
IUUC-Oru-084828	Oryza rufipogon	33.72	5.00e-41	159.00
IUUC-Osa-085937	Oryza sativa	39.00	6.00e-38	149.00
IUUC-Ola-086559	Oryzias latipes	50.00	2.00e-19	87.00
IUUC-Olu-087766	Ostreococcus lucimarinus	31.86	1.00e-33	134.00
IUUC-Oga-087983	Otolemur garnettii	33.15	2.00e-20	90.90
IUUC-Oar-089281	Ovis aries	32.97	3.00e-20	90.50
IUUC-Ptr-090983	Pan troglodytes	32.97	3.00e-20	90.90
IUUC-Pan-092457	Papio anubis	33.91	4.00e-20	90.10
IUUC-Psi-094046	Pelodiscus sinensis	32.97	6.00e-20	89.40
IUUC-Pno-094925	Phaeosphaeria nodorum	61.97	2.00e-106	377.00
IUUC-Ppa-095487	Physcomitrella patens	38.24	1.00e-35	141.00
IUUC-Pfo-096968	Poecilia formosa	33.15	5.00e-21	93.20
IUUC-Pab-098621	Pongo abelii	32.97	4.00e-19	87.00
IUUC-Pop-100007	Populus trichocarpa	31.45	5.00e-38	149.00
IUUC-Pca-100590	Procavia capensis	33.15	3.00e-20	90.50
IUUC-Ppe-101657	Prunus persica	31.69	1.00e-36	145.00
IUUC-Pva-102446	Pteropus vampyrus	32.97	3.00e-20	90.90
IUUC-Pgr-103604	Puccinia graminis	48.84	5.00e-21	93.60
IUUC-Ptt-103941	Puccinia triticina	47.25	5.00e-21	93.60
IUUC-Pte-104252	Pyrenophora teres	61.24	3.00e-109	386.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	60.67	3.00e-108	383.00
IUUC-Rno-106025	Rattus norvegicus	32.97	3.00e-20	90.50
IUUC-Sce-106172	Saccharomyces cerevisiae	37.02	4.00e-52	196.00
IUUC-Sha-106858	Sarcophilus harrisii	33.91	4.00e-20	90.10
IUUC-Sja-107905	Schizosaccharomyces japonicus	33.33	4.00e-19	86.30
IUUC-Spo-108185	Schizosaccharomyces pombe	53.85	6.00e-19	85.90
IUUC-Smo-108883	Selaginella moellendorffii	30.98	3.00e-34	137.00
IUUC-Sit-110512	Setaria italica	34.10	1.00e-36	144.00
IUUC-Sly-111238	Solanum lycopersicum	32.00	5.00e-39	152.00
IUUC-Stu-112441	Solanum tuberosum	32.53	2.00e-38	151.00
IUUC-Sar-113039	Sorex araneus	33.15	1.00e-20	91.70
IUUC-Sbi-113776	Sorghum bicolor	36.88	1.00e-34	137.00
IUUC-Sre-115074	Sporisorium reilianum	51.19	5.00e-20	90.50
IUUC-Ssc-115262	Sus scrofa	32.97	3.00e-20	90.50
IUUC-Tgu-116790	Taeniopygia guttata	32.97	3.00e-20	90.50
IUUC-Tru-118160	Takifugu rubripes	50.00	2.00e-19	88.20
IUUC-Tsy-119145	Tarsius syrichta	57.89	4.00e-08	50.40
IUUC-Tni-119937	Tetraodon nigroviridis	28.93	1.00e-30	124.00
IUUC-Tca-121323	Theobroma cacao	31.52	3.00e-38	150.00
IUUC-Tre-121993	Trichoderma reesei	54.92	2.00e-93	333.00
IUUC-Tvi-122332	Trichoderma virens	54.19	5.00e-92	328.00
IUUC-Tae-123478	Triticum aestivum	36.33	8.00e-34	135.00
IUUC-Tur-126679	Triticum urartu	35.94	2.00e-33	134.00
IUUC-Tme-127159	Tuber melanosporum	56.32	1.00e-19	88.60
IUUC-Tbe-127590	Tupaia belangeri	25.26	1.90e-02	30.80
IUUC-Ttr-128261	Tursiops truncatus	32.97	3.00e-20	90.50
IUUC-Uma-129572	Ustilago maydis	33.67	7.00e-24	103.00
IUUC-Vda-129966	Verticillium dahliae	64.99	6.00e-109	385.00
IUUC-Vpa-130698	Vicugna pacos	32.97	4.00e-20	90.10
IUUC-Vvi-131240	Vitis vinifera	25.00	5.00e-03	32.70
IUUC-Xtr-132878	Xenopus tropicalis	30.56	9.00e-36	142.00
IUUC-Xma-134017	Xiphophorus maculatus	32.61	1.00e-20	91.70
IUUC-Yli-134373	Yarrowia lipolytica	44.00	8.00e-33	132.00
IUUC-Zma-135222	Zea mays	38.57	8.00e-20	89.40

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence