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Tag Content
UUCD2 ID IUUC-Bta-013349
UUCD1 version UUC-BoT-01147
Ensembl Protein ID ENSBTAP00000053221.2
UniProt Accession E1BMF7; UBP13_BOVIN
Genbank Protein ID ENSBTAP00000053221
Protein Name Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13
Genbank Nucleotide ID DAAA02001954
Gene Name USP13
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSBTAG00000005946.6 ENSBTAT00000061390.2 ENSBTAP00000053221.2
Status Unreviewed
Classification
Family E-Value Score Start End
DUB/USP 2.10e-57 195.0 336 860
UBD/Alpha-Helix/UBA 5.80e-18 64.5 730 767
Active Site
Position(s) Description Evidence
345 Nucleophile ECO:0000255|PROSITE-ProRule:PRU10092,
ECO:0000255|PROSITE-ProRule:PRU10093}
823 Proton acceptor ECO:0000255|PROSITE-ProRule:PRU10092,
ECO:0000255|PROSITE-ProRule:PRU10093}
Domain Profile

   DUB/USP
   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeel......................wkskkka 67
           tGlkNlgN+CY+ Sv+Q++++ipe+++++  + ++ ++ + p +p++++ ++++kl + l                       k +++ 
   Q: 336  TGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYS-PLDPTQDFNTQMTKLGHGLlsgqyskppvkselieqvmkeeHKPQQNG 423
           7******************************788777765.79*****************8888888888888877765543388888* PP
   S: 68   vsprellkalkkeaeefsgyeqqDaqEflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikc 156
           +spr++++ ++k ++efs+++qqDaqEf+ +l++ +e++                               +++  sd+F++ ++++i+c
   Q: 424  ISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNR-----------------------------IGSENPSDVFRFLVEERIQC 483
           *************************************998.............................688899************** PP
   S: 157  lecdnvssreepfldlslpvpgkeetkke...........................slqdcLesftkeeelegenkyycekckkkqeae 218
            + ++v  +e   + ++lpv ++  t+k                             ++ cL++f+++e++++   ++++  ++k+  +
   Q: 484  CQTRKVRYTERVDYLMQLPVAMEAATNKDeliayeltrreaesnrrplpelvrakiPFSACLQAFSEPENVDD---FWSSALQAKSAGV 569
           ******9999999999999999998887799****************************************97...************* PP
   S: 219  kklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfcts........................................ 267
           k+ ++ ++Pe+L++++k+f+f  + ++++k++ +v++p+ Ld+++  ++                                        
   Q: 570  KTSRFASFPEYLVVQIKKFTFGLD-WVPKKFDVSVDMPDLLDINHLRARglqpgeeelpdisppivipddskdrlmtqlidpsdidess 657
           *********************997.*******************999889999999999999999998888888888888888888888 PP
   S: 268  ......................................................................................... 267
                                                                                                    
   Q: 658  vmqlaemgfpleacrkavyftgnmgaevafnwivvhmeepdfaepltmpgyggaasagasvfgatgldnqppeetvaiitsmgfhrnqa 746
           88888888877777777777777777777777777777777777777777777777777777777777766666666666666666666 PP
   S: 268  ...........peekqke...........................................kkkYeLiavvvHsG.slssGHYvayvkn 301
                      +     +                                           +  YeL+a ++H+G s+ sGHYv+++k+
   Q: 747  iqalratnsnlE-----RaldwifshpefeedsdfviemennananivseakpegprvkdgSGMYELFAFISHMGtSTMSGHYVCHIKK 830
           666655555550.....05555555555555555666666666667788888888888888**************************** PP
   S: 302  ekgkWllfdDskVtevkeeevleetgstaYvlfYkr 337
            +g+W+ ++D+kV ++  e+  +   + +Y++fY+r
   Q: 831  -EGRWVIYNDHKVCAS--ERPPK---DLGYMYFYRR 860
           .9***********999..78888...99******98 PP
   

   UBD/Alpha-Helix/UBA
   S: 4    edkleqlveMGFdreealqaLraannnleaAveyLldg 41
           e+ ++ +++MGF r++a+qaLra+n nle+A+++++++
   Q: 730  EETVAIITSMGFHRNQAIQALRATNSNLERALDWIFSH 767
           789999******************************97 PP
   
Organism Bos taurus
Functional Description
(View)

Functional Description


     Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
 Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Protein Sequence
(Fasta)
MQRRGALFGM PGGSGSRKMA AGDIGELLVP HMPTIRVPRS GDRVYKNECA FSYDSPNSEG 60
GLYVCMNTFL AFGREHVERH FRKTGQSVYM HLKRHVREKV RGASGGALPK RRNSKMFLDL 120
It may take some time, please wait.

Protein Fasta Sequence


>IUUC-Bta-013349|DUB,USP;UBD,UBA|Bos taurus
Please wait for a moment...
Nucleotide Sequence
(Fasta)
CCCGCCGCCG CCGCCGCCGC CGCCGCCGCC GAGGTGGGCA GACCCAGCGC TCAGCTCGGC 60
TGGAGTGCGC GCCGCGGCCA TGCAGCGCCG GGGCGCCCTG TTCGGCATGC CGGGCGGTAG 120
It may take some time, please wait.

Nucleotide Fasta Sequence


>IUUC-Bta-013349|DUB,USP;UBD,UBA|Bos taurus
Please wait for a moment...
Sequence Source Ensembl
Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0677--Repeat
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR001394--Peptidase_C19_UCH
IPR015940--UBA
IPR009060--UBA-like
IPR016652--Ubiquitinyl_hydrolase
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP
PROSITE

PS50030--UBA
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP
Pfam

PF00627--UBA
PF00443--UCH
PF02148--zf-UBP
SMART

SM00165--UBA
SM00290--ZnF_UBP
Gene Ontology

GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0043130--F:ubiquitin binding
GO:1904454--F:ubiquitin-specific protease activity involved in positive regulation of ERAD pathway
GO:0008270--F:zinc ion binding
GO:0006914--P:autophagy
GO:0008283--P:cell proliferation
GO:1904378--P:maintenance of unfolded protein involved in ERAD pathway
GO:0035523--P:protein K29-linked deubiquitination
GO:0044313--P:protein K6-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination
GO:0050821--P:protein stabilization
GO:0010506--P:regulation of autophagy
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006511--P:ubiquitin-dependent protein catabolic process
KEGG bta:100141289
Orthology
iUUCD ID Species Identity E-value Score
IUUC-Aml-001356 Ailuropoda melanoleuca 97.90 0.00e+00 1611.00
IUUC-Apl-003712 Anas platyrhynchos 83.35 0.00e+00 1350.00
IUUC-Aca-004634 Anolis carolinensis 82.97 0.00e+00 1399.00
IUUC-Ame-011470 Astyanax mexicanus 74.56 0.00e+00 1289.00
IUUC-Cja-019021 Callithrix jacchus 96.52 0.00e+00 1591.00
IUUC-Cfa-020614 Canis familiaris 97.91 0.00e+00 1617.00
IUUC-Cpo-022484 Cavia porcellus 97.17 0.00e+00 1503.00
IUUC-Csa-024078 Chlorocebus sabaeus 98.03 0.00e+00 1618.00
IUUC-Cho-024522 Choloepus hoffmanni 80.42 0.00e+00 1173.00
IUUC-Dre-027651 Danio rerio 74.94 0.00e+00 1353.00
IUUC-Dno-028975 Dasypus novemcinctus 97.69 0.00e+00 1613.00
IUUC-Dor-030130 Dipodomys ordii 72.79 0.00e+00 1059.00
IUUC-Ete-032251 Echinops telfairi 82.28 0.00e+00 1274.00
IUUC-Eca-033568 Equus caballus 98.39 0.00e+00 1508.00
IUUC-Eeu-034612 Erinaceus europaeus 79.31 0.00e+00 1148.00
IUUC-Fca-036280 Felis catus 98.26 0.00e+00 1507.00
IUUC-Fal-036610 Ficedula albicollis 84.02 0.00e+00 1326.00
IUUC-Gmo-038906 Gadus morhua 72.64 0.00e+00 1257.00
IUUC-Gga-041215 Gallus gallus 82.77 0.00e+00 1425.00
IUUC-Gac-042441 Gasterosteus aculeatus 71.89 0.00e+00 1273.00
IUUC-Ggo-044446 Gorilla gorilla 97.80 0.00e+00 1618.00
IUUC-Hsa-046546 Homo sapiens 98.03 0.00e+00 1620.00
IUUC-Itr-048147 Ictidomys tridecemlineatus 88.85 0.00e+00 1427.00
IUUC-Lch-050425 Latimeria chalumnae 77.62 0.00e+00 1356.00
IUUC-Loc-052072 Lepisosteus oculatus 77.36 0.00e+00 1312.00
IUUC-Laf-053909 Loxodonta africana 95.61 0.00e+00 1597.00
IUUC-Mcc-054686 Macaca mulatta 98.03 0.00e+00 1619.00
IUUC-Meu-056361 Macropus eugenii 79.74 0.00e+00 1302.00
IUUC-Mga-059972 Meleagris gallopavo 78.75 0.00e+00 1294.00
IUUC-Mmr-060916 Microcebus murinus 97.45 0.00e+00 1614.00
IUUC-Mdo-062302 Monodelphis domestica 88.57 0.00e+00 1506.00
IUUC-Mmu-063639 Mus musculus 94.79 0.00e+00 1597.00
IUUC-Mpu-066533 Mustela putorius furo 98.26 0.00e+00 1622.00
IUUC-Mlu-067786 Myotis lucifugus 91.90 0.00e+00 1476.00
IUUC-Nle-069997 Nomascus leucogenys 97.87 0.00e+00 1488.00
IUUC-Ont-071859 Oreochromis niloticus 72.58 0.00e+00 1276.00
IUUC-Oan-072959 Ornithorhynchus anatinus 88.28 2.00e-128 451.00
IUUC-Ocu-074265 Oryctolagus cuniculus 96.97 0.00e+00 1590.00
IUUC-Ola-087344 Oryzias latipes 71.58 0.00e+00 1280.00
IUUC-Oga-088734 Otolemur garnettii 97.72 0.00e+00 1551.00
IUUC-Oar-089636 Ovis aries 99.50 0.00e+00 1595.00
IUUC-Ptr-090982 Pan troglodytes 98.15 0.00e+00 1622.00
IUUC-Pan-091991 Papio anubis 98.15 0.00e+00 1620.00
IUUC-Psi-093509 Pelodiscus sinensis 86.13 0.00e+00 1457.00
IUUC-Pfo-096029 Poecilia formosa 74.28 0.00e+00 1325.00
IUUC-Pab-098672 Pongo abelii 93.74 0.00e+00 1520.00
IUUC-Pca-100860 Procavia capensis 73.53 0.00e+00 1033.00
IUUC-Pva-102545 Pteropus vampyrus 89.92 0.00e+00 1473.00
IUUC-Rno-105210 Rattus norvegicus 94.09 0.00e+00 1576.00
IUUC-Sar-113733 Sorex araneus 96.04 0.00e+00 771.00
IUUC-Tgu-116591 Taeniopygia guttata 84.14 0.00e+00 1429.00
IUUC-Tru-118627 Takifugu rubripes 70.94 0.00e+00 1289.00
IUUC-Tsy-119278 Tarsius syrichta 88.85 0.00e+00 1345.00
IUUC-Tni-120804 Tetraodon nigroviridis 73.76 0.00e+00 1315.00
IUUC-Tbe-127699 Tupaia belangeri 78.86 0.00e+00 875.00
IUUC-Ttr-128295 Tursiops truncatus 89.93 0.00e+00 1477.00
IUUC-Vpa-130170 Vicugna pacos 86.74 0.00e+00 1331.00
IUUC-Xtr-132809 Xenopus tropicalis 74.13 0.00e+00 1274.00
Created Date 25-Jun-2017

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