iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
Protein-protein Interaction
PINA	HINT
Post-translational Modifications (PTMs)
dbPPT
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	4.10e-168	560.7	475	962

Active Site

Position(s)	Description	Evidence
656	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGv.....gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveah 93
           YD Qi ++G++ q+kl+ akv+ vG g+lGcE+lKnL+l+Gv     g++tv+D+d +e+snl+rqFLfr++++g+ K+ vaa a++ +np  n+ea 
   Q: 475  YDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVscgsqGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRFNIEAL 572
           ************************************************************************************************** PP
   S: 94   eesiteleeeiv...FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsddppqktiPfctlketpnaaehtie 188
           ++++ +++e+++   F ++++vVv+aldn+ ar++v+++cl+ ++pl+esgtlG++ +++ +i++lte y +s+dpp+k++P+ct+ ++p +++h+++
   Q: 573  QNRVGAETENVFddaFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 670
           **********99************************************************************************************** PP
   S: 189  wa............vlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttinsikqllksfecdivtkskspfwv 274
           wa            + +n +l+   + ++       ++ +++++++ + l++e+ e+f+++ ++a+  f+ ++ n +kql+  f+ d  t+ ++pfw+
   Q: 671  WArsefegllektpAEVNAYLSSPVEYTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPEDAATSTGAPFWS 768
           *********9999977777777777777777778889999999999999************************************************* PP
   S: 275  .........................................................sfcsnaealqvpekvekdeevvkasap..lylllralerfe 313
                                                                     f++ ++a+ v +  +   ++ +++++  +  l++++++ +
   Q: 769  apkrfprplqysssdpsllnfitatailraetfgipipewtknpkeaaeavdrvivpDFEPRQDAKIVTDEKATTLTTASVDDAavIDDLIAKIDQCR 866
           *****************************************999999999999999977777766665555555555555555568777777777665 PP
   S: 314  k..kkgrkpgelsfekddds..avdlvtaaanlraeslgiepkldddlikeiagniipaiattnavvgGlaaqEviKvvsg..kfeplknfflfdae 404
           +   ++  ++ ++fekddd   ++d+++ +an+ra +++i++ +d+ + k iag+iipaiat +a+  Gl+++E++Kv++g  k e+++n+f ++a+
   Q: 867  HnlSPDFRMKPIQFEKDDDTnyHMDVIAGLANMRARNYSIPE-VDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGghKVEAYRNTFANLAL 962
           5116889999********************************.***************************************9*********99875 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Protein Sequence
(Fasta)

MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR GFHGDSVVQQ 60
IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI SGMHGLGAEI AKNLILAGVK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TCACAATCAC GCGGAGAAAG AGCGACAAAT CAAACCTTAA CCGTTAAAAC GTCATCGTTT 60
CATATCCCCC CACAACCGAA CCCCTTTCGT TCGCCCGTCT CTCTTAAATA AATCTTAGAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR032420--E1_4HB
IPR032418--E1_FCCH
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR018965--Ub-activating_enz_E1_C
IPR019572--UBA_E1_Cys
IPR018075--UBQ-activ_enz_E1
IPR018074--UBQ-activ_enz_E1_CS
IPR033127--UBQ-activ_enz_E1_Cys_AS
IPR000011--UBQ/SUMO-activ_enz_E1-like

PROSITE

PS00536--UBIQUITIN_ACTIVAT_1
PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF16191--E1_4HB
PF16190--E1_FCCH
PF09358--E1_UFD
PF00899--ThiF
PF10585--UBA_e1_thiolCys

PRINTS

PR01849--UBIQUITINACT

SMART

SM00985--UBA_e1_C

Gene Ontology

GO:0005829--C:cytosol
GO:0005886--C:plasma membrane
GO:0009506--C:plasmodesma
GO:0005524--F:ATP binding
GO:0008641--F:small protein activating enzyme activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0016567--P:protein ubiquitination
GO:0046686--P:response to cadmium ion
GO:0051707--P:response to other organism

KEGG

ath:AT2G30110

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aly-005418	Arabidopsis lyrata	95.38	0.00e+00	2093.00
IUUC-Acl-008181	Aspergillus clavatus	45.03	0.00e+00	808.00
IUUC-Bol-015880	Brassica oleracea	92.51	0.00e+00	2026.00
IUUC-Bra-017252	Brassica rapa	95.49	0.00e+00	2011.00
IUUC-Cne-026960	Cryptococcus neoformans	45.03	0.00e+00	856.00
IUUC-Cme-027290	Cyanidioschyzon merolae	38.71	0.00e+00	683.00
IUUC-Dme-032045	Drosophila melanogaster	44.60	0.00e+00	844.00
IUUC-Gma-043165	Glycine max	83.61	0.00e+00	1795.00
IUUC-Mtr-058572	Medicago truncatula	83.20	0.00e+00	1707.00
IUUC-Mvi-060437	Microbotryum violaceum	43.38	0.00e+00	815.00
IUUC-Olu-087687	Ostreococcus lucimarinus	58.66	0.00e+00	1127.00
IUUC-Pma-094214	Petromyzon marinus	46.78	7.00e-35	139.00
IUUC-Pop-099577	Populus trichocarpa	79.22	0.00e+00	1761.00
IUUC-Ppe-101298	Prunus persica	81.43	0.00e+00	1693.00
IUUC-Sja-107841	Schizosaccharomyces japonicus	44.10	0.00e+00	843.00
IUUC-Smo-108912	Selaginella moellendorffii	72.87	0.00e+00	1513.00
IUUC-Sly-111237	Solanum lycopersicum	80.37	0.00e+00	1642.00
IUUC-Stu-112001	Solanum tuberosum	80.88	0.00e+00	1654.00
IUUC-Tca-121436	Theobroma cacao	83.97	0.00e+00	1779.00
IUUC-Vvi-130857	Vitis vinifera	84.07	0.00e+00	1769.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence