iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
microRNA	TRANSFAC	miRNAMap	RAID2
Protein-protein Interaction
IID	Mentha
Post-translational Modifications (PTMs)
dbPAF
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	46.28	2.00e-63	239.00	IUUC-Ath-006802
E2/Autophagy_C	97.13	8.00e-171	597.00	IUUC-Hsa-046449
E2/Autophagy_C	34.57	7.00e-38	152.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
264	Glycyl thioester intermediate	{ECO:0000250}

Organism

Rattus norvegicus

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (By similarity).

Protein Sequence
(Fasta)

MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 60
LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 120

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Nucleotide Sequence
(Fasta)

GGGAAGCCGG AAGGGGTTCA AGTGGAGCTT GGGGGACGAA AGACAGCTTC GAGAAGGCCG 60
GGGGCTGCGC GCGCACCCGT CTCTCGCTCA GCCGGCTGGG CCTCTGCTGT CGCGGCCAGG 120

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Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0019777--F:Atg12 transferase activity
GO:0019776--F:Atg8 ligase activity
GO:0016874--F:ligase activity
GO:0000045--P:autophagosome assembly
GO:0006914--P:autophagy
GO:0043653--P:mitochondrial fragmentation involved in apoptotic process
GO:0000422--P:mitophagy
GO:0050765--P:negative regulation of phagocytosis
GO:0044804--P:nucleophagy
GO:0015031--P:protein transport
GO:0016567--P:protein ubiquitination
GO:1902017--P:regulation of cilium assembly

KEGG

rno:171415

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	44.19	2.00e-47	182.00
IUUC-Aml-002081	Ailuropoda melanoleuca	96.82	4.00e-170	590.00
IUUC-Atr-002712	Amborella trichopoda	44.28	2.00e-51	195.00
IUUC-Apl-003956	Anas platyrhynchos	75.32	2.00e-119	422.00
IUUC-Aca-004578	Anolis carolinensis	88.57	2.00e-159	554.00
IUUC-Aly-005823	Arabidopsis lyrata	45.95	7.00e-65	240.00
IUUC-Ath-006802	Arabidopsis thaliana	46.28	2.00e-65	242.00
IUUC-Ago-007855	Ashbya gossypii	37.91	5.00e-23	101.00
IUUC-Acl-008147	Aspergillus clavatus	50.63	6.00e-17	81.60
IUUC-Afl-008483	Aspergillus flavus	37.36	6.00e-33	134.00
IUUC-Afu-008818	Aspergillus fumigatus	32.97	1.00e-19	90.90
IUUC-Ani-009481	Aspergillus nidulans	51.76	9.00e-18	84.30
IUUC-Ang-009800	Aspergillus niger	37.84	5.00e-21	95.10
IUUC-Aor-010150	Aspergillus oryzae	37.36	6.00e-33	134.00
IUUC-Ate-010545	Aspergillus terreus	53.95	4.00e-17	81.60
IUUC-Ame-011793	Astyanax mexicanus	82.76	3.00e-152	530.00
IUUC-Bgr-012167	Blumeria graminis	48.84	2.00e-17	83.20
IUUC-Bta-012695	Bos taurus	97.77	2.00e-172	597.00
IUUC-Bci-013972	Botrytis cinerea	54.05	3.00e-18	85.90
IUUC-Bdi-014756	Brachypodium distachyon	45.27	1.00e-58	219.00
IUUC-Bol-015091	Brassica oleracea	43.63	8.00e-61	227.00
IUUC-Bra-017220	Brassica rapa	44.84	1.00e-62	233.00
IUUC-Cel-018143	Caenorhabditis elegans	50.80	2.00e-81	295.00
IUUC-Cja-018991	Callithrix jacchus	93.02	2.00e-172	598.00
IUUC-Cfa-020070	Canis familiaris	97.42	1.00e-169	588.00
IUUC-Cpo-021860	Cavia porcellus	97.13	1.00e-171	595.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	40.65	1.00e-64	239.00
IUUC-Csa-023881	Chlorocebus sabaeus	96.82	6.00e-171	592.00
IUUC-Cho-024852	Choloepus hoffmanni	84.39	2.00e-154	538.00
IUUC-Cin-025787	Ciona intestinalis	63.17	3.00e-107	380.00
IUUC-Csv-026369	Ciona savignyi	63.29	8.00e-107	379.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	35.03	1.00e-19	90.50
IUUC-Cne-026905	Cryptococcus neoformans	48.11	3.00e-26	112.00
IUUC-Dre-028811	Danio rerio	79.81	1.00e-150	525.00
IUUC-Dno-030081	Dasypus novemcinctus	92.72	1.00e-165	575.00
IUUC-Dor-030183	Dipodomys ordii	98.47	6.00e-74	270.00
IUUC-Dse-031539	Dothistroma septosporum	43.48	1.00e-11	64.30
IUUC-Dme-031805	Drosophila melanogaster	61.21	6.00e-103	367.00
IUUC-Ete-033171	Echinops telfairi	91.40	3.00e-164	570.00
IUUC-Eca-034083	Equus caballus	98.09	1.00e-172	598.00
IUUC-Eeu-034804	Erinaceus europaeus	94.59	5.00e-164	570.00
IUUC-Fca-036289	Felis catus	97.45	4.00e-172	597.00
IUUC-Fal-037060	Ficedula albicollis	92.04	1.00e-159	555.00
IUUC-Fox-038051	Fusarium oxysporum	40.34	2.00e-18	86.70
IUUC-Fso-038124	Fusarium solani	57.35	2.00e-19	89.40
IUUC-Gmo-039538	Gadus morhua	80.13	2.00e-144	504.00
IUUC-Ggr-039880	Gaeumannomyces graminis	34.55	3.00e-20	92.80
IUUC-Gga-041108	Gallus gallus	91.40	2.00e-158	551.00
IUUC-Gac-041558	Gasterosteus aculeatus	81.59	5.00e-147	513.00
IUUC-Gma-043969	Glycine max	46.58	5.00e-63	234.00
IUUC-Ggo-045071	Gorilla gorilla	97.13	3.00e-172	597.00
IUUC-Hsa-046449	Homo sapiens	97.13	3.00e-172	597.00
IUUC-Hvu-047451	Hordeum vulgare	46.15	2.00e-58	219.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	98.09	1.00e-172	598.00
IUUC-Kpa-049096	Komagataella pastoris	39.33	8.00e-25	107.00
IUUC-Lch-049726	Latimeria chalumnae	82.80	7.00e-156	542.00
IUUC-Lpe-050734	Leersia perrieri	45.15	7.00e-51	194.00
IUUC-Loc-051983	Lepisosteus oculatus	84.76	1.00e-148	518.00
IUUC-Lma-053038	Leptosphaeria maculans	50.62	4.00e-17	82.00
IUUC-Laf-054510	Loxodonta africana	88.05	3.00e-158	550.00
IUUC-Mcc-054792	Macaca mulatta	96.82	6.00e-171	592.00
IUUC-Meu-055965	Macropus eugenii	84.08	6.00e-139	486.00
IUUC-Mor-057292	Magnaporthe oryzae	30.48	1.00e-36	147.00
IUUC-Mtr-058282	Medicago truncatula	45.45	9.00e-65	240.00
IUUC-Mla-059144	Melampsora laricipopulina	64.10	2.00e-26	113.00
IUUC-Mga-059509	Meleagris gallopavo	91.08	5.00e-158	550.00
IUUC-Mvi-060281	Microbotryum violaceum	36.48	3.00e-47	182.00
IUUC-Mmr-061788	Microcebus murinus	97.13	5.00e-171	593.00
IUUC-Mdo-062922	Monodelphis domestica	95.22	3.00e-168	583.00
IUUC-Mmu-064249	Mus musculus	98.41	9.00e-173	598.00
IUUC-Mac-064843	Musa acuminata	45.72	3.00e-63	235.00
IUUC-Mpu-065717	Mustela putorius furo	96.82	8.00e-168	582.00
IUUC-Mlu-067151	Myotis lucifugus	94.30	5.00e-155	539.00
IUUC-Nfi-068551	Neosartorya fischeri	34.55	8.00e-36	144.00
IUUC-Ncr-068846	Neurospora crassa	35.40	3.00e-34	138.00
IUUC-Nle-069790	Nomascus leucogenys	97.13	3.00e-172	597.00
IUUC-Ont-072517	Oreochromis niloticus	84.08	7.00e-148	516.00
IUUC-Oan-073285	Ornithorhynchus anatinus	88.36	1.00e-102	365.00
IUUC-Ocu-074695	Oryctolagus cuniculus	98.09	5.00e-173	599.00
IUUC-Oba-075515	Oryza barthii	45.76	3.00e-63	235.00
IUUC-Obr-076787	Oryza brachyantha	46.15	2.00e-62	233.00
IUUC-Ogl-077440	Oryza glaberrima	43.99	2.00e-63	235.00
IUUC-Ogu-078294	Oryza glumaepatula	45.76	2.00e-63	236.00
IUUC-Oin-079452	Oryza indica	46.15	2.00e-63	236.00
IUUC-Olo-080450	Oryza longistaminata	45.67	3.00e-62	231.00
IUUC-Ome-081514	Oryza meridionalis	45.21	2.00e-63	235.00
IUUC-Oni-082677	Oryza nivara	46.15	2.00e-63	236.00
IUUC-Opu-083925	Oryza punctata	45.55	3.00e-63	235.00
IUUC-Oru-084828	Oryza rufipogon	45.67	1.00e-62	233.00
IUUC-Osa-085937	Oryza sativa	46.15	2.00e-63	236.00
IUUC-Ola-086559	Oryzias latipes	79.64	2.00e-91	328.00
IUUC-Olu-087766	Ostreococcus lucimarinus	40.84	5.00e-58	217.00
IUUC-Oga-087983	Otolemur garnettii	96.19	5.00e-170	590.00
IUUC-Oar-089281	Ovis aries	98.09	2.00e-172	597.00
IUUC-Ptr-090983	Pan troglodytes	97.13	3.00e-172	597.00
IUUC-Pan-092457	Papio anubis	96.50	2.00e-170	590.00
IUUC-Psi-094046	Pelodiscus sinensis	87.93	6.00e-146	509.00
IUUC-Pno-094925	Phaeosphaeria nodorum	35.18	5.00e-31	128.00
IUUC-Ppa-095487	Physcomitrella patens	45.39	2.00e-64	238.00
IUUC-Pfo-096968	Poecilia formosa	84.81	1.00e-148	518.00
IUUC-Pab-098621	Pongo abelii	96.82	7.00e-170	589.00
IUUC-Pop-100007	Populus trichocarpa	47.44	3.00e-65	241.00
IUUC-Pca-100590	Procavia capensis	78.98	9.00e-133	466.00
IUUC-Ppe-101657	Prunus persica	46.62	1.00e-63	236.00
IUUC-Pva-102446	Pteropus vampyrus	97.45	5.00e-172	596.00
IUUC-Pgr-103604	Puccinia graminis	58.33	6.00e-26	111.00
IUUC-Ptt-103941	Puccinia triticina	59.76	8.00e-26	111.00
IUUC-Pte-104252	Pyrenophora teres	54.67	2.00e-17	83.60
IUUC-Pyt-104556	Pyrenophora triticirepentis	54.67	2.00e-17	83.20
IUUC-Sce-106172	Saccharomyces cerevisiae	34.57	7.00e-38	150.00
IUUC-Sha-106858	Sarcophilus harrisii	95.54	2.00e-167	580.00
IUUC-Sja-107905	Schizosaccharomyces japonicus	47.83	1.00e-27	116.00
IUUC-Spo-108185	Schizosaccharomyces pombe	45.00	3.00e-19	89.00
IUUC-Smo-108883	Selaginella moellendorffii	44.04	6.00e-56	211.00
IUUC-Sit-110512	Setaria italica	46.28	6.00e-60	224.00
IUUC-Sly-111238	Solanum lycopersicum	46.67	1.00e-67	249.00
IUUC-Stu-112441	Solanum tuberosum	46.98	3.00e-68	251.00
IUUC-Sar-113039	Sorex araneus	83.70	6.00e-121	426.00
IUUC-Sbi-113776	Sorghum bicolor	46.92	3.00e-58	218.00
IUUC-Sre-115074	Sporisorium reilianum	52.94	4.00e-22	99.40
IUUC-Ssc-115262	Sus scrofa	97.77	5.00e-172	596.00
IUUC-Tgu-116790	Taeniopygia guttata	91.72	4.00e-159	553.00
IUUC-Tru-118160	Takifugu rubripes	77.53	3.00e-142	497.00
IUUC-Tsy-119145	Tarsius syrichta	78.34	1.00e-126	446.00
IUUC-Tni-119937	Tetraodon nigroviridis	74.92	2.00e-135	474.00
IUUC-Tca-121323	Theobroma cacao	46.62	4.00e-62	231.00
IUUC-Tre-121993	Trichoderma reesei	45.63	7.00e-20	91.30
IUUC-Tvi-122332	Trichoderma virens	36.50	6.00e-34	137.00
IUUC-Tae-124391	Triticum aestivum	45.82	3.00e-59	222.00
IUUC-Tur-126679	Triticum urartu	45.82	2.00e-58	219.00
IUUC-Tme-127159	Tuber melanosporum	38.24	1.00e-11	63.50
IUUC-Ttr-128261	Tursiops truncatus	97.24	4.00e-158	550.00
IUUC-Uma-129572	Ustilago maydis	48.11	6.00e-23	102.00
IUUC-Vda-129966	Verticillium dahliae	31.75	2.00e-38	152.00
IUUC-Vpa-130698	Vicugna pacos	86.67	5.00e-152	530.00
IUUC-Xtr-132878	Xenopus tropicalis	85.40	5.00e-154	536.00
IUUC-Xma-134017	Xiphophorus maculatus	84.18	6.00e-148	516.00
IUUC-Yli-134373	Yarrowia lipolytica	35.92	7.00e-20	91.30
IUUC-Zma-135222	Zea mays	43.83	4.00e-61	228.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence