iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	circBase	miRTarBase	microRNA
TRANSFAC	RepTar	miRNAMap	RAID2
Protein-protein Interaction
IID	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
UniProt	PHOSIDA	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	12532266

Classification

Family	E-value	Score	Start	End
DUB/USP	6.40e-120	401.5	289	933
ULD/UDP/UBQ/UBQ_Other	5.00e-09	37.0	147	217

Active Site

Position(s)	Description	Evidence
298	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
891	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle.kkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgye 88
           +Gl+NlgNtC+mNS++QcLsn+p+l+e++l+ k++e+ln +np ++ ge++ks+++l++++w++k ++v+pr+++++++++a++fsgy+
   Q: 289  CGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNdKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQ 377
           7******************************9********************************************************* PP
   S: 89   qqDaqEflaflldqleeelkkeknkpkeeeeeedkselsde.....aeekkkkknkslisdlFqgqlksqikclecdnvssreepfldl 172
           qqD+qE+laflld+l+e+l++ ++kp+ +++++d   ++d+     a+e++ k+n+s+i+d+F+g +ks+++c+ec+++s++++pf++l
   Q: 378  QQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDAD--GRPDKvvaeeAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYL 464
           ******************************9999..5555555777******************************************* PP
   S: 173  slpvpgkeetkke............................................................................ 185
           +lp+p+k+e+++e                                                                            
   Q: 465  TLPLPMKKERSLEvylvrmdplakpmqykvivpkignildlctalsalsgvpadkmivtdiynhrfhrifavdenlssimerddiyvfe 553
           **********999**************************************************************************** PP
   S: 186  ......................................................................................... 185
                                                                                                    
   Q: 554  ininraedtehvvipvclrekfrhssythhtgsslfgqpflmaiprnntedklynllllrmcryvkmsteteetdghlrccedqningn 642
           ***************************************************************************************** PP
   S: 186  ......................................................................................... 185
                                                                                                    
   Q: 643  gpnglheegspsemetdepddessqdqelpsenensqsedsvggdndsenglcteetckgqltghkkrlftfqfnnlgnndinyikddt 731
           ***************************************************************************************** PP
   S: 186  .......................................................slqdcLesftkeeelegenkyycekckkkqeaek 219
                                                                  +l+dc+e ft++e+l  e+ +yc++ck++q+a+k
   Q: 732  shirfddrqlrldersflaldwdpdlkkryfdenaaedfekhesveykppkrpfvKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATK 820
           ***************************************************************************************** PP
   S: 220  klklkklPevLilhLkRfsfseeqksrekinkkvefple.LdlspfctspeekqkekkkYeLiavvvHsGslssGHYvayvknekgk.W 306
           kl+l++lP+vL++hLkRfs+s+  ++r k+++ v+fp++ Ld+s+f+++p+ +   +++Y+Liav +H+G++++GHY+a++kn++++ W
   Q: 821  KLDLWSLPPVLVVHLKRFSYSR--YMRDKLDTLVDFPISdLDMSEFLINPNAG---PCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGkW 904
           ******************9977..6999*********************9988...8**************************65555* PP
   S: 307  llfdDskVtevkeeevleetgstaYvlfYkrr 338
           ++fdDs+V++ +e+++++   + aYvlfY+r+
   Q: 905  YYFDDSSVSTASEDQIVS---KAAYVLFYQRQ 933
           ******************...*********96 PP

ULD/UDP/UBQ/UBQ_Other

   S: 12   eltlkvnpsdtVaeLKekiekklgip.peqqrLiyk.....GkiL.eddktLadyglkdgstihllekksg 75
           + t++++++dt+ ++ ++i+k ++ip + + rL++k      ++L ++d t++d gl++g+++++++k+++
   Q: 147  VVTRRFSKADTIDTIEKEIRKIFNIPdEKEARLWNKymsntFEPLnKPDSTIQDAGLYQGQVLVIEQKNED 217
           77899*********************999*****998875544569*********************9986 PP

Organism

Mus musculus

Functional Description
(View)

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP.

Protein Sequence
(Fasta)

MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV 60
YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCTACGGCTA GTGGAAGAAG ATGGCGGAAG GCGGAGCGGC GGACCTGGAC ACCCAGCGTT 60
CTGACATCGC GACGCTGCTC AAAACCTCGC TCCGGAAAGG GGACACCTGG TAGGGAACGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR006615--Pept_C19_DUSP
IPR001394--Peptidase_C19_UCH
IPR013792--RNA3'P_cycl/enolpyr_Trfase_a/b
IPR028135--Ub_USP-typ
IPR029071--Ubiquitin-rel_dom
IPR029346--USP_C
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS51283--DUSP
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF06337--DUSP
PF14836--Ubiquitin_3
PF00443--UCH
PF14533--USP7_C2

SMART

SM00695--DUSP

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0004197--F:cysteine-type endopeptidase activity
GO:0042802--F:identical protein binding
GO:0046332--F:SMAD binding
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0005160--F:transforming growth factor beta receptor binding
GO:0061649--F:ubiquitinated histone binding
GO:0030509--P:BMP signaling pathway
GO:0035616--P:histone H2B conserved C-terminal lysine deubiquitination
GO:0035520--P:monoubiquitinated protein deubiquitination
GO:0060389--P:pathway-restricted SMAD protein phosphorylation
GO:0016579--P:protein deubiquitination
GO:0007179--P:transforming growth factor beta receptor signaling pathway
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

mmu:14479

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000058	Aegilops tauschii	40.03	2.00e-119	423.00
IUUC-Aml-001852	Ailuropoda melanoleuca	97.35	0.00e+00	1872.00
IUUC-Apl-003249	Anas platyrhynchos	93.49	0.00e+00	1702.00
IUUC-Aca-004261	Anolis carolinensis	92.51	0.00e+00	1726.00
IUUC-Aly-005644	Arabidopsis lyrata	35.91	2.00e-138	486.00
IUUC-Afl-008507	Aspergillus flavus	43.30	1.00e-53	206.00
IUUC-Aor-009949	Aspergillus oryzae	42.96	1.00e-52	202.00
IUUC-Ame-010757	Astyanax mexicanus	78.05	0.00e+00	1248.00
IUUC-Bgr-012277	Blumeria graminis	44.12	7.00e-63	236.00
IUUC-Bta-013128	Bos taurus	94.09	0.00e+00	1837.00
IUUC-Bci-013993	Botrytis cinerea	43.49	3.00e-64	241.00
IUUC-Bol-015570	Brassica oleracea	37.15	3.00e-154	539.00
IUUC-Cja-019222	Callithrix jacchus	97.55	0.00e+00	1875.00
IUUC-Cfa-020035	Canis familiaris	97.35	0.00e+00	1870.00
IUUC-Cpo-022071	Cavia porcellus	97.18	0.00e+00	1786.00
IUUC-Csa-023882	Chlorocebus sabaeus	97.76	0.00e+00	1880.00
IUUC-Csv-026109	Ciona savignyi	43.80	0.00e+00	702.00
IUUC-Cgl-026741	Colletotrichum gloeosporioides	36.41	7.00e-61	230.00
IUUC-Cme-027338	Cyanidioschyzon merolae	56.36	1.00e-67	251.00
IUUC-Dno-029025	Dasypus novemcinctus	91.29	0.00e+00	1753.00
IUUC-Dor-030956	Dipodomys ordii	88.81	0.00e+00	1608.00
IUUC-Dse-031432	Dothistroma septosporum	40.43	1.00e-58	222.00
IUUC-Eca-034340	Equus caballus	97.79	0.00e+00	1819.00
IUUC-Eeu-034488	Erinaceus europaeus	87.47	0.00e+00	1244.00
IUUC-Fca-035552	Felis catus	94.19	0.00e+00	1832.00
IUUC-Fal-036618	Ficedula albicollis	93.39	0.00e+00	1697.00
IUUC-Gga-041146	Gallus gallus	92.47	0.00e+00	1745.00
IUUC-Gac-041679	Gasterosteus aculeatus	77.16	0.00e+00	1457.00
IUUC-Ggo-045002	Gorilla gorilla	97.48	0.00e+00	1815.00
IUUC-Hsa-046052	Homo sapiens	97.66	0.00e+00	1878.00
IUUC-Hvu-047108	Hordeum vulgare	39.88	9.00e-123	434.00
IUUC-Itr-048306	Ictidomys tridecemlineatus	94.40	0.00e+00	1837.00
IUUC-Lch-050107	Latimeria chalumnae	85.55	0.00e+00	1712.00
IUUC-Loc-052105	Lepisosteus oculatus	83.28	0.00e+00	1504.00
IUUC-Lma-052981	Leptosphaeria maculans	38.44	1.00e-65	246.00
IUUC-Laf-053645	Loxodonta africana	97.15	0.00e+00	1868.00
IUUC-Mcc-055586	Macaca mulatta	97.66	0.00e+00	1878.00
IUUC-Meu-056764	Macropus eugenii	87.78	0.00e+00	984.00
IUUC-Mga-060162	Meleagris gallopavo	92.78	0.00e+00	1749.00
IUUC-Mmr-061105	Microcebus murinus	97.76	0.00e+00	1876.00
IUUC-Mdo-062107	Monodelphis domestica	95.93	0.00e+00	1821.00
IUUC-Mac-064956	Musa acuminata	40.54	3.00e-109	389.00
IUUC-Mpu-065868	Mustela putorius furo	95.40	0.00e+00	1862.00
IUUC-Mlu-068000	Myotis lucifugus	91.16	0.00e+00	1663.00
IUUC-Nle-069874	Nomascus leucogenys	97.68	0.00e+00	1854.00
IUUC-Opr-071212	Ochotona princeps	91.98	0.00e+00	1757.00
IUUC-Ont-071834	Oreochromis niloticus	79.26	0.00e+00	1451.00
IUUC-Ocu-074573	Oryctolagus cuniculus	97.86	0.00e+00	1879.00
IUUC-Oba-075427	Oryza barthii	34.56	9.00e-133	468.00
IUUC-Ogu-078422	Oryza glumaepatula	34.56	8.00e-133	468.00
IUUC-Oni-082834	Oryza nivara	34.67	3.00e-133	469.00
IUUC-Opu-083691	Oryza punctata	35.10	2.00e-145	509.00
IUUC-Oru-084820	Oryza rufipogon	34.56	8.00e-133	468.00
IUUC-Osa-086139	Oryza sativa	36.00	1.00e-147	517.00
IUUC-Oga-089022	Otolemur garnettii	94.09	0.00e+00	1834.00
IUUC-Oar-089767	Ovis aries	97.15	0.00e+00	1871.00
IUUC-Ptr-091319	Pan troglodytes	94.80	0.00e+00	1848.00
IUUC-Pan-091968	Papio anubis	97.76	0.00e+00	1880.00
IUUC-Psi-093849	Pelodiscus sinensis	92.24	0.00e+00	1694.00
IUUC-Pma-094533	Petromyzon marinus	59.75	0.00e+00	1113.00
IUUC-Pno-094890	Phaeosphaeria nodorum	36.12	3.00e-59	224.00
IUUC-Pfo-097110	Poecilia formosa	78.28	0.00e+00	1451.00
IUUC-Pab-098664	Pongo abelii	97.76	0.00e+00	1880.00
IUUC-Pca-100359	Procavia capensis	79.35	0.00e+00	1314.00
IUUC-Pva-102394	Pteropus vampyrus	95.72	0.00e+00	1830.00
IUUC-Pgr-103546	Puccinia graminis	29.12	8.00e-68	253.00
IUUC-Pte-104102	Pyrenophora teres	35.77	1.00e-57	219.00
IUUC-Pyt-104812	Pyrenophora triticirepentis	39.75	6.00e-57	217.00
IUUC-Rno-105383	Rattus norvegicus	96.02	0.00e+00	1861.00
IUUC-Sha-107393	Sarcophilus harrisii	95.83	0.00e+00	1828.00
IUUC-Sja-107752	Schizosaccharomyces japonicus	34.16	1.00e-80	294.00
IUUC-Ssl-108461	Sclerotinia sclerotiorum	30.56	1.00e-13	71.60
IUUC-Smo-108686	Selaginella moellendorffii	47.75	3.00e-80	293.00
IUUC-Sly-111726	Solanum lycopersicum	39.74	1.00e-92	335.00
IUUC-Sar-113498	Sorex araneus	96.94	0.00e+00	1864.00
IUUC-Sre-115130	Sporisorium reilianum	32.67	9.00e-88	319.00
IUUC-Ssc-115285	Sus scrofa	97.66	0.00e+00	1873.00
IUUC-Tgu-116529	Taeniopygia guttata	92.98	0.00e+00	1753.00
IUUC-Tru-118298	Takifugu rubripes	78.41	0.00e+00	1459.00
IUUC-Tsy-119117	Tarsius syrichta	87.87	0.00e+00	1135.00
IUUC-Tni-120093	Tetraodon nigroviridis	75.26	0.00e+00	1395.00
IUUC-Tre-121888	Trichoderma reesei	42.81	9.00e-63	236.00
IUUC-Tvi-122422	Trichoderma virens	39.93	4.00e-57	217.00
IUUC-Tae-125811	Triticum aestivum	41.62	4.00e-126	445.00
IUUC-Tur-126149	Triticum urartu	39.50	2.00e-105	377.00
IUUC-Tbe-127672	Tupaia belangeri	90.04	0.00e+00	1235.00
IUUC-Ttr-128441	Tursiops truncatus	93.65	0.00e+00	1150.00
IUUC-Vpa-130469	Vicugna pacos	97.48	0.00e+00	1805.00
IUUC-Xtr-132729	Xenopus tropicalis	83.21	0.00e+00	1612.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence