iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Rno-105622

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

NEDD8-conjugating enzyme UBE2F; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; RING-type E3 NEDD8 transferase UBE2F; Ubiquitin-conjugating enzyme E2 F

Genbank Nucleotide ID

DY311915

Gene Name

Ube2f

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000019953.7	ENSRNOT00000027050.7	ENSRNOP00000027050.4
ENSRNOG00000019953.7	ENSRNOT00000042082.4	ENSRNOP00000040354.2

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
microRNA	TRANSFAC	miRNAMap	RAID2
Protein-protein Interaction
IID
Post-translational Modifications (PTMs)
CPLM	PhosphositePlus
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	8.40e-37	126.4	37	177

Active Site

Position(s)	Description	Evidence
116	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 2    lkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilk... 87
           l ke++ele++ p ++++++ d ++l+ +++++  p++ +Y+gg+F++e e+p+ Y++ PPkvk+ltki+hPn++e+G++Cls+l+   
   Q: 37   LVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVS-PDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLRehs 124
           679********************99********9.**************************************************7778 PP
   S: 88   .eeekWspalsvesvllsiqsllae.pnpesplneeaaellkknreeykkkvr 138
            + + W p+ ++++v+++++sl+++  n+++pln eaae++ +++e+++ kv+
   Q: 125  iDGTGWAPTRTLKDVVWGLNSLFTDlLNFDDPLNIEAAEHHLRDKEDFRDKVD 177
           8899*******************8879**********************9996 PP

Organism

Rattus norvegicus

Functional Description
(View)

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Protein Sequence
(Fasta)

MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPC TCKVHFPDPN 60
KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCTAACTC TGGCAAGCAA GTTGAAGCGG GATGATGGTC TCAAAGGATC CCGGGCCTCA 60
GCCTCCACAT CCGACTCCAC TCGGAGGGTT TCTGTGAGAG ACAAGTTGCT TGTTAAAGGT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005524--F:ATP binding
GO:0019788--F:NEDD8 transferase activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0045116--P:protein neddylation

KEGG

rno:363284

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001473	Ailuropoda melanoleuca	94.59	3.00e-92	330.00
IUUC-Apl-003569	Anas platyrhynchos	85.71	3.00e-48	182.00
IUUC-Aca-005152	Anolis carolinensis	90.81	2.00e-98	351.00
IUUC-Ame-011839	Astyanax mexicanus	79.46	1.00e-88	318.00
IUUC-Bta-013524	Bos taurus	95.68	1.00e-92	332.00
IUUC-Cel-018579	Caenorhabditis elegans	57.81	2.00e-35	142.00
IUUC-Cja-019211	Callithrix jacchus	96.76	7.00e-96	342.00
IUUC-Cfa-020014	Canis familiaris	94.59	3.00e-92	330.00
IUUC-Cpo-022479	Cavia porcellus	96.22	7.00e-96	342.00
IUUC-Cho-024583	Choloepus hoffmanni	64.32	3.00e-60	224.00
IUUC-Cin-025378	Ciona intestinalis	48.37	4.00e-52	197.00
IUUC-Csv-026195	Ciona savignyi	51.37	2.00e-50	191.00
IUUC-Dre-028671	Danio rerio	81.08	7.00e-90	322.00
IUUC-Dno-029004	Dasypus novemcinctus	95.14	2.00e-105	374.00
IUUC-Dor-030940	Dipodomys ordii	95.14	2.00e-95	340.00
IUUC-Eca-034345	Equus caballus	95.14	1.00e-92	332.00
IUUC-Fca-036125	Felis catus	94.59	3.00e-92	330.00
IUUC-Fal-036808	Ficedula albicollis	91.89	3.00e-101	360.00
IUUC-Gmo-039394	Gadus morhua	88.50	7.00e-60	221.00
IUUC-Gga-041269	Gallus gallus	87.63	6.00e-94	335.00
IUUC-Gac-041480	Gasterosteus aculeatus	82.70	3.00e-89	320.00
IUUC-Ggo-045010	Gorilla gorilla	96.22	4.00e-106	376.00
IUUC-Hsa-046664	Homo sapiens	96.22	4.00e-106	376.00
IUUC-Itr-048522	Ictidomys tridecemlineatus	96.22	9.00e-95	338.00
IUUC-Loc-052137	Lepisosteus oculatus	83.24	1.00e-91	328.00
IUUC-Laf-053694	Loxodonta africana	94.59	7.00e-97	345.00
IUUC-Mcc-055638	Macaca mulatta	96.22	4.00e-106	376.00
IUUC-Meu-056568	Macropus eugenii	77.30	4.00e-80	290.00
IUUC-Mga-059437	Meleagris gallopavo	92.97	3.00e-101	360.00
IUUC-Mmr-060651	Microcebus murinus	92.78	5.00e-90	323.00
IUUC-Mdo-061993	Monodelphis domestica	91.89	3.00e-100	357.00
IUUC-Mmu-064372	Mus musculus	99.46	3.00e-97	346.00
IUUC-Mpu-066423	Mustela putorius furo	94.05	6.00e-92	329.00
IUUC-Mlu-067910	Myotis lucifugus	95.14	1.00e-92	332.00
IUUC-Nle-069032	Nomascus leucogenys	95.68	7.00e-96	342.00
IUUC-Opr-070798	Ochotona princeps	96.22	2.00e-105	374.00
IUUC-Ont-071232	Oreochromis niloticus	82.70	1.00e-89	322.00
IUUC-Oan-072779	Ornithorhynchus anatinus	90.91	6.00e-65	238.00
IUUC-Ocu-074366	Oryctolagus cuniculus	91.35	7.00e-102	362.00
IUUC-Ola-087548	Oryzias latipes	88.14	2.00e-61	227.00
IUUC-Oga-089149	Otolemur garnettii	96.76	4.00e-98	349.00
IUUC-Oar-089662	Ovis aries	95.68	1.00e-92	332.00
IUUC-Ptr-090895	Pan troglodytes	96.22	4.00e-106	376.00
IUUC-Pan-092771	Papio anubis	95.70	8.00e-105	372.00
IUUC-Psi-093582	Pelodiscus sinensis	91.89	6.00e-101	359.00
IUUC-Pma-094321	Petromyzon marinus	84.21	5.00e-67	245.00
IUUC-Pfo-096040	Poecilia formosa	83.78	5.00e-91	326.00
IUUC-Pab-097931	Pongo abelii	96.22	4.00e-106	376.00
IUUC-Pca-100697	Procavia capensis	94.48	6.00e-83	298.00
IUUC-Pva-102609	Pteropus vampyrus	94.05	3.00e-93	333.00
IUUC-Sha-107614	Sarcophilus harrisii	91.40	1.00e-99	354.00
IUUC-Ssc-115597	Sus scrofa	94.59	5.00e-95	339.00
IUUC-Tgu-117397	Taeniopygia guttata	91.89	3.00e-101	360.00
IUUC-Tru-118496	Takifugu rubripes	81.08	2.00e-87	314.00
IUUC-Tsy-119592	Tarsius syrichta	96.22	9.00e-98	348.00
IUUC-Tni-119759	Tetraodon nigroviridis	81.62	3.00e-88	317.00
IUUC-Tbe-128085	Tupaia belangeri	79.26	2.00e-60	223.00
IUUC-Ttr-129228	Tursiops truncatus	94.59	8.00e-92	328.00
IUUC-Xtr-131773	Xenopus tropicalis	91.24	3.00e-62	230.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence