iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Dre-028671

UUCD1 version

UUC-DaR-01370

Ensembl Protein ID

ENSDARP00000120196.1

UniProt Accession

Q6NY82; B7ZD57; B7ZD58; UBE2F_DANRE

Genbank Protein ID

CAX12885.1; CAX12886.1; AAH66702.1

Protein Name

NEDD8-conjugating enzyme UBE2F; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; RING-type E3 NEDD8 transferase UBE2F; Ubiquitin-conjugating enzyme E2 F

Genbank Nucleotide ID

AL929396; AL929396; BC066702

Gene Name

ube2f; ch211-93g23.1; zgc:77005

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSDARG00000025754.8	ENSDART00000144641.2	ENSDARP00000120196.1
ENSDARG00000025754.8	ENSDART00000138919.1	ENSDARP00000121227.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	7.10e-37	127.2	37	177

Active Site

Position(s)	Description	Evidence
116	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 2    lkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilk... 87
           l ke++ele++ p+++++++ de++l +++++i  p++ +Y gg+F++eie+pe Y++ PPkvk+lt+i+hPn+ e+G++Cls+l+   
   Q: 37   LIKEVAELEANLPSTCKVTFPDENKLCHFQLAIS-PDEGYYLGGKFQFEIEVPEAYNMVPPKVKCLTRIWHPNIAETGEICLSLLRehs 124
           679********************9*********9.**************************************************7778 PP
   S: 88   .eeekWspalsvesvllsiqsllae.pnpesplneeaaellkknreeykkkvr 138
            + + W p+ ++++v+++++sl+++  n+++pln +aae++ +++e++++kv+
   Q: 125  iDGTGWAPTRTLKDVVWGLNSLFTDlLNFDDPLNIDAAEHHLRDKEDFRNKVQ 177
           8899*******************8879***********************997 PP

Organism

Danio rerio

Functional Description
(View)

Accepts the ubiquitin-like protein NEDD8 from the uba3-nae1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx2, but not rbx1, suggests that the rbx2-ube2f complex neddylates specific target proteins, such as cul5.

Protein Sequence
(Fasta)

MLTLASKLKR EEGVRAGRTP AGSNDAAHRV SIRDRLLIKE VAELEANLPS TCKVTFPDEN 60
KLCHFQLAIS PDEGYYLGGK FQFEIEVPEA YNMVPPKVKC LTRIWHPNIA ETGEICLSLL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGAACAACTT CCGGCTTCTA AACCGCTCAG GCAGCGGAAA TCATTAAACT CGGCGGAACT 60
AATCCTCGCA TCGTTCCACT CAATTTTGTT TCTTTTATCG ACGTTCAGCA TATTTTATCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005524--F:ATP binding
GO:0019788--F:NEDD8 transferase activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0045116--P:protein neddylation

KEGG

dre:406606

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001473	Ailuropoda melanoleuca	83.78	2.00e-81	295.00
IUUC-Apl-003569	Anas platyrhynchos	79.05	6.00e-47	178.00
IUUC-Aca-005152	Anolis carolinensis	80.00	6.00e-88	316.00
IUUC-Ame-011839	Astyanax mexicanus	91.89	9.00e-100	355.00
IUUC-Bta-013524	Bos taurus	82.70	3.00e-81	293.00
IUUC-Cel-018579	Caenorhabditis elegans	53.12	2.00e-32	131.00
IUUC-Cja-019211	Callithrix jacchus	82.16	1.00e-82	298.00
IUUC-Cfa-020014	Canis familiaris	83.78	2.00e-81	295.00
IUUC-Cpo-022479	Cavia porcellus	82.70	1.00e-82	298.00
IUUC-Cho-024583	Choloepus hoffmanni	55.68	8.00e-53	199.00
IUUC-Cin-025378	Ciona intestinalis	47.83	5.00e-52	196.00
IUUC-Csv-026195	Ciona savignyi	52.25	1.00e-50	192.00
IUUC-Dno-029004	Dasypus novemcinctus	82.70	2.00e-91	328.00
IUUC-Dor-030940	Dipodomys ordii	81.08	1.00e-81	295.00
IUUC-Eca-034345	Equus caballus	83.24	3.00e-81	293.00
IUUC-Fca-036125	Felis catus	82.70	1.00e-80	292.00
IUUC-Fal-036808	Ficedula albicollis	81.08	3.00e-90	323.00
IUUC-Gmo-039394	Gadus morhua	94.69	4.00e-63	232.00
IUUC-Gga-041269	Gallus gallus	78.49	4.00e-84	303.00
IUUC-Gac-041480	Gasterosteus aculeatus	83.78	2.00e-92	331.00
IUUC-Ggo-045010	Gorilla gorilla	82.16	7.00e-91	326.00
IUUC-Hsa-046664	Homo sapiens	82.16	7.00e-91	326.00
IUUC-Itr-048522	Ictidomys tridecemlineatus	82.16	4.00e-82	296.00
IUUC-Loc-052137	Lepisosteus oculatus	86.49	9.00e-95	338.00
IUUC-Laf-053694	Loxodonta africana	81.62	1.00e-84	305.00
IUUC-Mcc-055638	Macaca mulatta	82.16	7.00e-91	326.00
IUUC-Meu-056568	Macropus eugenii	68.11	9.00e-72	262.00
IUUC-Mga-059437	Meleagris gallopavo	82.70	5.00e-91	326.00
IUUC-Mmr-060651	Microcebus murinus	78.89	1.00e-77	282.00
IUUC-Mdo-061993	Monodelphis domestica	82.70	5.00e-91	327.00
IUUC-Mmu-064372	Mus musculus	81.62	7.00e-82	296.00
IUUC-Mpu-066423	Mustela putorius furo	83.24	3.00e-81	293.00
IUUC-Mlu-067910	Myotis lucifugus	83.24	4.00e-81	293.00
IUUC-Nle-069032	Nomascus leucogenys	81.62	1.00e-82	298.00
IUUC-Opr-070798	Ochotona princeps	81.62	4.00e-90	323.00
IUUC-Ont-071232	Oreochromis niloticus	84.86	1.00e-93	335.00
IUUC-Oan-072779	Ornithorhynchus anatinus	88.43	2.00e-62	230.00
IUUC-Ocu-074366	Oryctolagus cuniculus	80.00	1.00e-88	318.00
IUUC-Ola-087548	Oryzias latipes	91.23	6.00e-62	228.00
IUUC-Oga-089149	Otolemur garnettii	82.16	1.00e-84	305.00
IUUC-Oar-089662	Ovis aries	82.70	3.00e-81	293.00
IUUC-Ptr-090895	Pan troglodytes	82.16	7.00e-91	326.00
IUUC-Pan-092771	Papio anubis	81.72	2.00e-89	321.00
IUUC-Psi-093582	Pelodiscus sinensis	82.70	5.00e-91	326.00
IUUC-Pma-094321	Petromyzon marinus	83.46	2.00e-65	241.00
IUUC-Pfo-096040	Poecilia formosa	85.41	2.00e-94	337.00
IUUC-Pab-097931	Pongo abelii	82.16	7.00e-91	326.00
IUUC-Pca-100697	Procavia capensis	86.90	3.00e-74	270.00
IUUC-Pva-102609	Pteropus vampyrus	83.78	5.00e-83	300.00
IUUC-Rno-105622	Rattus norvegicus	81.08	7.00e-82	296.00
IUUC-Sha-107614	Sarcophilus harrisii	81.72	6.00e-90	322.00
IUUC-Ssc-115597	Sus scrofa	81.08	9.00e-82	295.00
IUUC-Tgu-117397	Taeniopygia guttata	81.62	1.00e-90	325.00
IUUC-Tru-118496	Takifugu rubripes	83.78	3.00e-92	330.00
IUUC-Tsy-119592	Tarsius syrichta	82.70	5.00e-85	306.00
IUUC-Tni-119759	Tetraodon nigroviridis	84.32	2.00e-92	331.00
IUUC-Tbe-128085	Tupaia belangeri	71.11	6.00e-52	196.00
IUUC-Ttr-129228	Tursiops truncatus	82.16	6.00e-81	293.00
IUUC-Xtr-131773	Xenopus tropicalis	79.56	1.00e-55	208.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence