iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Acl-008147

Ensembl Protein ID

CADACLAP00000792

UniProt Accession

A1CAN8; ATG3_ASPCL

Genbank Protein ID

EAW12806.1

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3

Genbank Nucleotide ID

DS027049

Gene Name

ACLA_012340; atg3

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
CADACLAG00000811	CADACLAT00000811	CADACLAP00000792

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	31.89	6.00e-30	128.00	IUUC-Ath-006802
E2/Autophagy_C	35.96	2.00e-28	124.00	IUUC-Hsa-046449
E2/Autophagy_C	37.01	4.00e-51	196.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
247	Glycyl thioester intermediate	{ECO:0000250}

Organism

Aspergillus clavatus

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNILHSTLST WRDRLAPVSR TSTFRTTGQI TPEEFVLAGD YLVYKFPSWS WADASSPAKR 60
VSYLPPGKQF LVTRGVPCHR RLNENFAGDA GHEDEIVRDM LAGDDGDGWL RTGGGRDLAE 120

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Nucleotide Sequence
(Fasta)

ATGAACATCC TCCATTCCAC CCTCTCAACC TGGCGGGATC GCCTGGCCCC CGTCTCGCGC 60
ACCTCCACCT TCCGCACCAC CGGTCAAATC ACCCCAGAAG AGTTCGTCCT CGCCGGCGAC 120

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Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

KEGG

act:ACLA_012340

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	35.14	2.00e-29	120.00
IUUC-Aml-002081	Ailuropoda melanoleuca	45.54	4.00e-20	90.10
IUUC-Atr-002712	Amborella trichopoda	42.34	7.00e-19	85.90
IUUC-Apl-003956	Anas platyrhynchos	31.03	3.00e-18	83.60
IUUC-Aca-004578	Anolis carolinensis	35.58	1.00e-34	138.00
IUUC-Aly-005823	Arabidopsis lyrata	32.35	2.00e-40	157.00
IUUC-Ath-006802	Arabidopsis thaliana	31.89	4.00e-39	153.00
IUUC-Ago-007855	Ashbya gossypii	35.83	3.00e-53	199.00
IUUC-Afl-008483	Aspergillus flavus	78.18	1.00e-144	503.00
IUUC-Afu-008818	Aspergillus fumigatus	85.32	1.00e-168	583.00
IUUC-Ani-009481	Aspergillus nidulans	76.94	2.00e-137	479.00
IUUC-Ang-009800	Aspergillus niger	71.78	5.00e-137	478.00
IUUC-Aor-010150	Aspergillus oryzae	78.18	1.00e-144	503.00
IUUC-Ate-010545	Aspergillus terreus	70.24	2.00e-77	280.00
IUUC-Ame-011793	Astyanax mexicanus	45.45	6.00e-19	86.30
IUUC-Bgr-012167	Blumeria graminis	62.47	1.00e-101	361.00
IUUC-Bta-012695	Bos taurus	45.54	3.00e-20	90.50
IUUC-Bci-013972	Botrytis cinerea	59.31	6.00e-96	342.00
IUUC-Bdi-014756	Brachypodium distachyon	58.33	4.00e-15	73.60
IUUC-Bol-015091	Brassica oleracea	33.24	2.00e-39	154.00
IUUC-Bra-016662	Brassica rapa	33.33	2.00e-20	91.30
IUUC-Cel-018143	Caenorhabditis elegans	33.45	2.00e-36	144.00
IUUC-Cja-018991	Callithrix jacchus	34.70	5.00e-36	142.00
IUUC-Cfa-020070	Canis familiaris	45.54	3.00e-20	90.10
IUUC-Cpo-021860	Cavia porcellus	45.54	2.00e-20	91.30
IUUC-Cre-022948	Chlamydomonas reinhardtii	35.79	9.00e-36	142.00
IUUC-Csa-023881	Chlorocebus sabaeus	35.96	2.00e-36	144.00
IUUC-Cho-024852	Choloepus hoffmanni	44.55	2.00e-19	87.80
IUUC-Cin-025787	Ciona intestinalis	33.96	3.00e-35	140.00
IUUC-Csv-026369	Ciona savignyi	52.86	7.00e-20	89.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	56.82	6.00e-93	332.00
IUUC-Cne-026905	Cryptococcus neoformans	50.55	1.00e-19	88.60
IUUC-Dre-028811	Danio rerio	36.94	9.00e-36	142.00
IUUC-Dno-030081	Dasypus novemcinctus	37.17	6.00e-35	139.00
IUUC-Dor-030183	Dipodomys ordii	46.53	2.00e-20	90.90
IUUC-Dse-031539	Dothistroma septosporum	52.57	3.00e-63	233.00
IUUC-Dme-031805	Drosophila melanogaster	48.81	6.00e-19	86.30
IUUC-Ete-033171	Echinops telfairi	37.31	4.00e-36	142.00
IUUC-Eca-034083	Equus caballus	45.54	3.00e-20	90.10
IUUC-Eeu-034804	Erinaceus europaeus	46.53	2.00e-20	91.30
IUUC-Fca-036289	Felis catus	45.54	3.00e-20	90.10
IUUC-Fal-037060	Ficedula albicollis	46.53	3.00e-19	87.40
IUUC-Fox-038051	Fusarium oxysporum	60.00	2.00e-104	370.00
IUUC-Fso-038124	Fusarium solani	60.55	1.00e-92	331.00
IUUC-Gmo-039538	Gadus morhua	38.55	3.00e-35	140.00
IUUC-Ggr-039880	Gaeumannomyces graminis	58.01	2.00e-106	376.00
IUUC-Gga-041108	Gallus gallus	37.69	2.00e-35	141.00
IUUC-Gac-041558	Gasterosteus aculeatus	36.16	2.00e-36	144.00
IUUC-Gma-043969	Glycine max	31.88	3.00e-39	153.00
IUUC-Ggo-045071	Gorilla gorilla	35.96	3.00e-36	143.00
IUUC-Hsa-046449	Homo sapiens	35.96	3.00e-36	143.00
IUUC-Hvu-047451	Hordeum vulgare	36.29	7.00e-32	129.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	45.54	3.00e-20	90.10
IUUC-Kpa-049096	Komagataella pastoris	37.77	7.00e-55	205.00
IUUC-Lch-049726	Latimeria chalumnae	34.77	2.00e-36	144.00
IUUC-Lpe-050734	Leersia perrieri	38.13	8.00e-38	149.00
IUUC-Loc-051983	Lepisosteus oculatus	34.96	4.00e-29	120.00
IUUC-Lma-053038	Leptosphaeria maculans	65.93	1.00e-108	384.00
IUUC-Laf-054510	Loxodonta africana	43.40	5.00e-19	86.30
IUUC-Mcc-054792	Macaca mulatta	35.96	2.00e-36	144.00
IUUC-Meu-055965	Macropus eugenii	45.65	2.00e-18	84.30
IUUC-Mor-057292	Magnaporthe oryzae	60.27	2.00e-102	363.00
IUUC-Mtr-058282	Medicago truncatula	30.89	5.00e-39	152.00
IUUC-Mla-059144	Melampsora laricipopulina	44.26	2.00e-24	104.00
IUUC-Mga-059509	Meleagris gallopavo	38.29	1.00e-35	141.00
IUUC-Mvi-060281	Microbotryum violaceum	37.44	5.00e-26	110.00
IUUC-Mmr-061788	Microcebus murinus	36.02	9.00e-35	138.00
IUUC-Mdo-062922	Monodelphis domestica	45.54	5.00e-20	89.70
IUUC-Mmu-064249	Mus musculus	44.55	2.00e-19	87.40
IUUC-Mac-064474	Musa acuminata	30.79	7.00e-37	145.00
IUUC-Mpu-065717	Mustela putorius furo	45.54	4.00e-20	90.10
IUUC-Mlu-067151	Myotis lucifugus	47.83	9.00e-19	85.50
IUUC-Nfi-068551	Neosartorya fischeri	84.49	6.00e-170	587.00
IUUC-Ncr-068846	Neurospora crassa	60.39	1.00e-104	371.00
IUUC-Nle-069790	Nomascus leucogenys	35.96	3.00e-36	143.00
IUUC-Ont-072517	Oreochromis niloticus	35.48	9.00e-35	138.00
IUUC-Oan-073285	Ornithorhynchus anatinus	46.07	1.00e-17	81.60
IUUC-Ocu-074695	Oryctolagus cuniculus	45.54	3.00e-20	90.50
IUUC-Oba-075515	Oryza barthii	38.67	1.00e-38	151.00
IUUC-Obr-076787	Oryza brachyantha	33.14	4.00e-40	156.00
IUUC-Ogl-077440	Oryza glaberrima	33.06	4.00e-41	159.00
IUUC-Ogu-078294	Oryza glumaepatula	33.62	1.00e-41	161.00
IUUC-Oin-079452	Oryza indica	33.14	2.00e-41	160.00
IUUC-Olo-080450	Oryza longistaminata	32.23	2.00e-38	150.00
IUUC-Ome-081514	Oryza meridionalis	33.89	6.00e-41	159.00
IUUC-Oni-082677	Oryza nivara	33.14	2.00e-41	160.00
IUUC-Opu-083925	Oryza punctata	33.33	8.00e-41	158.00
IUUC-Oru-084828	Oryza rufipogon	32.51	2.00e-37	147.00
IUUC-Osa-085937	Oryza sativa	38.04	1.00e-38	151.00
IUUC-Ola-086559	Oryzias latipes	44.25	2.00e-20	90.50
IUUC-Olu-087766	Ostreococcus lucimarinus	33.08	6.00e-31	125.00
IUUC-Oga-087983	Otolemur garnettii	42.20	2.00e-18	84.30
IUUC-Oar-089281	Ovis aries	45.54	3.00e-20	90.50
IUUC-Ptr-090983	Pan troglodytes	35.96	3.00e-36	143.00
IUUC-Pan-092457	Papio anubis	35.96	7.00e-37	145.00
IUUC-Psi-094046	Pelodiscus sinensis	45.65	2.00e-17	81.30
IUUC-Pno-094925	Phaeosphaeria nodorum	61.94	1.00e-105	374.00
IUUC-Ppa-095487	Physcomitrella patens	34.93	6.00e-36	142.00
IUUC-Pfo-096968	Poecilia formosa	43.36	1.00e-19	88.60
IUUC-Pab-098621	Pongo abelii	35.96	6.00e-35	139.00
IUUC-Pop-100007	Populus trichocarpa	32.80	3.00e-38	150.00
IUUC-Pca-100590	Procavia capensis	47.19	9.00e-18	82.00
IUUC-Ppe-101657	Prunus persica	31.78	1.00e-37	148.00
IUUC-Pva-102446	Pteropus vampyrus	45.54	4.00e-20	90.10
IUUC-Pgr-103604	Puccinia graminis	47.13	4.00e-21	94.00
IUUC-Ptt-103941	Puccinia triticina	45.65	4.00e-21	94.00
IUUC-Pte-104252	Pyrenophora teres	61.22	3.00e-107	379.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	62.60	1.00e-107	381.00
IUUC-Rno-106025	Rattus norvegicus	45.54	4.00e-20	90.10
IUUC-Sce-106172	Saccharomyces cerevisiae	37.30	4.00e-44	169.00
IUUC-Sha-106858	Sarcophilus harrisii	45.54	3.00e-20	90.50
IUUC-Sja-107905	Schizosaccharomyces japonicus	47.50	4.00e-17	79.70
IUUC-Spo-108185	Schizosaccharomyces pombe	52.56	2.00e-18	84.70
IUUC-Smo-108883	Selaginella moellendorffii	28.68	1.00e-32	131.00
IUUC-Sit-110512	Setaria italica	34.46	7.00e-22	95.90
IUUC-Sly-111238	Solanum lycopersicum	35.51	4.00e-38	149.00
IUUC-Stu-112441	Solanum tuberosum	32.09	3.00e-40	157.00
IUUC-Sar-113039	Sorex araneus	47.19	9.00e-18	82.00
IUUC-Sbi-113776	Sorghum bicolor	51.90	3.00e-19	87.00
IUUC-Sre-115074	Sporisorium reilianum	50.00	1.00e-19	89.40
IUUC-Ssc-115262	Sus scrofa	36.40	1.00e-34	138.00
IUUC-Tgu-116790	Taeniopygia guttata	46.53	3.00e-19	87.40
IUUC-Tru-118160	Takifugu rubripes	44.25	9.00e-21	92.00
IUUC-Tsy-119145	Tarsius syrichta	31.68	1.00e-08	52.00
IUUC-Tni-119937	Tetraodon nigroviridis	33.21	2.00e-30	124.00
IUUC-Tca-121323	Theobroma cacao	30.52	1.00e-38	151.00
IUUC-Tre-121993	Trichoderma reesei	52.30	5.00e-90	322.00
IUUC-Tvi-122332	Trichoderma virens	51.09	2.00e-89	320.00
IUUC-Tae-123478	Triticum aestivum	34.75	2.00e-29	121.00
IUUC-Tur-126679	Triticum urartu	35.14	3.00e-29	120.00
IUUC-Tme-127159	Tuber melanosporum	44.53	1.00e-19	88.20
IUUC-Tbe-127590	Tupaia belangeri	25.26	1.00e-02	31.60
IUUC-Ttr-128261	Tursiops truncatus	46.74	2.00e-18	84.00
IUUC-Uma-129572	Ustilago maydis	32.08	1.00e-23	102.00
IUUC-Vda-129966	Verticillium dahliae	62.78	7.00e-108	381.00
IUUC-Vpa-130698	Vicugna pacos	30.97	1.00e-24	104.00
IUUC-Vvi-131240	Vitis vinifera	25.26	3.00e-03	33.50
IUUC-Xtr-132878	Xenopus tropicalis	36.33	2.00e-34	137.00
IUUC-Xma-134017	Xiphophorus maculatus	43.36	1.00e-19	88.60
IUUC-Yli-134373	Yarrowia lipolytica	38.68	6.00e-54	202.00
IUUC-Zma-135222	Zea mays	51.90	7.00e-19	86.30

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence