iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
Protein-protein Interaction
iRefIndex	PINA	HINT
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	12215511

Classification

Family	E-value	Score	Start	End
E1	7.20e-132	441.4	24	531

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesit 98
           YDRQ+r+wGe++q++l++a+++l+++g++G+E lKnLvl+GvgsitvvD+++v++ +l+++F+++a++vg+sKA++++++l+eln++vn+++ ee+++
   Q: 24   YDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFIEENPD 121
           ************************************************************************************************** PP
   S: 99   eleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlket..........pna 182
           +l ++++ Ff++f++V++++  +++++k++r+c++a+v+l+ ++++Gl G+vr+++k++++++s++d   d++++++P+++lk++          p+a
   Q: 122  TLITTNPsFFSQFTLVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDhflDDLRLNNPWPELKSFvetidlnvsePAA 219
           *************************************************************************************777776666666* PP
   S: 183  aehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv..... 274
           a+++i+++v++ k++ee+a++++++ ++++eek+e+kd+vks+++s+de+n++eaie+a+k fa++ i+s +++l+++ +c++v++++s+fwv     
   Q: 220  AHKHIPYVVILVKMAEEWAQSHSGNLPSTREEKKEFKDLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSeVQKLIND-SCAEVNSNSSAFWVmvaal 316
           **********************************************************************9*****99.******************* PP
   S: 275  ......................................................................sfcsnaealq...........vpekvek 291
                                                                                 sfc+na++l+           ++++v++
   Q: 317  kefvlnegggeaplegsipdmtsstehyinlqkiylakaeadflvieervknilkkigrdpssipkptikSFCKNARKLKlcryrmvedefRNPSVTE 414
           **********************************************************************************99999999999***** PP
   S: 292  deevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagniipaiattnavvgGlaaqE 383
            +++++++++     +y+llra++rf++++++ pg+++  +d+d + +l+t+a++l  ++lg+++ +l+ddli+e++++++++i++++a+vgG+a+qE
   Q: 415  IQKYLADEDYsgamgFYILLRAADRFAANYNKFPGQFDGGMDED-ISRLKTTALSLL-TDLGCNGsVLPDDLIHEMCRFGASEIHVVSAFVGGIASQE 510
           ********************************************.**********99.**************************************** PP
   S: 384  viKvvsgkfeplknfflfdae 404
           viK+v+++f+p+  +++f+++
   Q: 511  VIKLVTKQFVPMLGTYIFNGI 531
           ************999999986 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Regulatory subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1. Plays an important role in auxin response (PubMed:8321287). Regulates the chromosomal localization of meiotic recombination by crossovers (COs) and subsequent synapsis, probably through the activation of a CRL4 complex (PubMed:25116939). Required for E3-mediated protein degradation in response to auxin, jasmonic acid and cold stress. Required for the COP1-COP10-CSN-mediated repression of photomorphogenesis in the dark (PubMed:12368504). May function redundantly with AXL1 in the RUB conjugating pathway (PubMed:17655650). Seems not to be functionally equivalent to AXL1 in vivo (PubMed:21311953).

Protein Sequence
(Fasta)

MQAVKRSRRH VEEEPTMVEP KTKYDRQLRI WGEVGQAALE EASICLLNCG PTGSEALKNL 60
VLGGVGSITV VDGSKVQFGD LGNNFMVDAK SVGQSKAKSV CAFLQELNDS VNAKFIEENP 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AAAAAATCAG TAAGATATTA ATATCGATTT TTTAATTTGG TAGATAACTT TTTTCAAACG 60
ATAAATTTCC TAAAAACAAT AAAATATTCG GAAATAAACA AAAACCCTGA TTGAAGAAAA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-0927--Auxin signaling pathway
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0019781--F:NEDD8 activating enzyme activity
GO:0000166--F:nucleotide binding
GO:0010252--P:auxin homeostasis
GO:0009734--P:auxin-activated signaling pathway
GO:0006281--P:DNA repair
GO:0009965--P:leaf morphogenesis
GO:0045116--P:protein neddylation
GO:0007131--P:reciprocal meiotic recombination
GO:0009735--P:response to cytokinin
GO:0009414--P:response to water deprivation

KEGG

ath:AT1G05180

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000764	Aegilops tauschii	65.65	0.00e+00	732.00
IUUC-Atr-002644	Amborella trichopoda	71.56	0.00e+00	768.00
IUUC-Aly-005466	Arabidopsis lyrata	95.56	0.00e+00	1072.00
IUUC-Ani-009443	Aspergillus nidulans	31.19	6.00e-67	247.00
IUUC-Bgr-012111	Blumeria graminis	32.58	6.00e-61	227.00
IUUC-Bci-013892	Botrytis cinerea	31.05	2.00e-58	219.00
IUUC-Bdi-014592	Brachypodium distachyon	66.09	0.00e+00	736.00
IUUC-Bol-016399	Brassica oleracea	90.65	0.00e+00	987.00
IUUC-Bra-018084	Brassica rapa	85.31	0.00e+00	909.00
IUUC-Cel-018456	Caenorhabditis elegans	31.68	2.00e-73	269.00
IUUC-Cgl-026428	Colletotrichum gloeosporioides	30.20	1.00e-57	216.00
IUUC-Cne-026884	Cryptococcus neoformans	30.43	6.00e-69	254.00
IUUC-Cme-027210	Cyanidioschyzon merolae	25.10	8.00e-15	74.70
IUUC-Dme-031683	Drosophila melanogaster	37.17	4.00e-102	364.00
IUUC-Fox-038037	Fusarium oxysporum	31.03	3.00e-66	245.00
IUUC-Fso-038387	Fusarium solani	32.18	1.00e-66	246.00
IUUC-Gmo-038562	Gadus morhua	40.04	2.00e-121	428.00
IUUC-Ggr-040005	Gaeumannomyces graminis	34.69	7.00e-55	207.00
IUUC-Gma-043610	Glycine max	78.24	0.00e+00	853.00
IUUC-Hvu-047790	Hordeum vulgare	63.08	2.00e-149	521.00
IUUC-Kpa-049309	Komagataella pastoris	34.67	3.00e-74	271.00
IUUC-Lpe-051535	Leersia perrieri	64.24	0.00e+00	658.00
IUUC-Lma-053112	Leptosphaeria maculans	33.87	4.00e-79	288.00
IUUC-Mor-057230	Magnaporthe oryzae	30.58	1.00e-64	239.00
IUUC-Mpo-057514	Magnaporthe poae	32.72	2.00e-62	233.00
IUUC-Mtr-058551	Medicago truncatula	73.89	0.00e+00	806.00
IUUC-Mvi-060221	Microbotryum violaceum	33.58	6.00e-74	271.00
IUUC-Mac-064521	Musa acuminata	68.46	0.00e+00	765.00
IUUC-Nfi-068245	Neosartorya fischeri	32.48	4.00e-73	268.00
IUUC-Ont-071426	Oreochromis niloticus	40.60	4.00e-127	447.00
IUUC-Oba-075437	Oryza barthii	68.39	0.00e+00	716.00
IUUC-Obr-076684	Oryza brachyantha	67.31	0.00e+00	669.00
IUUC-Ogl-077170	Oryza glaberrima	68.39	0.00e+00	716.00
IUUC-Ogu-078844	Oryza glumaepatula	66.80	0.00e+00	662.00
IUUC-Oin-079105	Oryza indica	68.20	0.00e+00	712.00
IUUC-Olo-080943	Oryza longistaminata	63.73	0.00e+00	641.00
IUUC-Oni-082891	Oryza nivara	66.48	0.00e+00	689.00
IUUC-Opu-083457	Oryza punctata	68.20	0.00e+00	714.00
IUUC-Oru-084960	Oryza rufipogon	63.73	0.00e+00	642.00
IUUC-Osa-085358	Oryza sativa	68.01	0.00e+00	709.00
IUUC-Ola-086420	Oryzias latipes	38.73	8.00e-79	286.00
IUUC-Olu-087625	Ostreococcus lucimarinus	32.42	1.00e-63	237.00
IUUC-Pno-095084	Phaeosphaeria nodorum	32.41	4.00e-65	243.00
IUUC-Ppa-095798	Physcomitrella patens	55.91	0.00e+00	627.00
IUUC-Pop-099722	Populus trichocarpa	78.63	0.00e+00	884.00
IUUC-Ppe-101280	Prunus persica	78.63	0.00e+00	877.00
IUUC-Pva-102333	Pteropus vampyrus	37.62	5.00e-116	410.00
IUUC-Pte-104355	Pyrenophora teres	33.09	5.00e-76	278.00
IUUC-Pyt-104545	Pyrenophora triticirepentis	33.93	6.00e-77	281.00
IUUC-Ssl-108387	Sclerotinia sclerotiorum	33.03	7.00e-62	230.00
IUUC-Sit-110157	Setaria italica	67.75	0.00e+00	776.00
IUUC-Sly-111105	Solanum lycopersicum	73.79	0.00e+00	845.00
IUUC-Stu-112469	Solanum tuberosum	75.09	0.00e+00	859.00
IUUC-Sbi-113813	Sorghum bicolor	67.56	0.00e+00	763.00
IUUC-Sre-115058	Sporisorium reilianum	34.75	3.00e-65	242.00
IUUC-Tru-118686	Takifugu rubripes	39.47	5.00e-124	437.00
IUUC-Tca-121491	Theobroma cacao	80.53	0.00e+00	864.00
IUUC-Tvi-122672	Trichoderma virens	32.08	3.00e-66	245.00
IUUC-Tae-125316	Triticum aestivum	66.48	0.00e+00	739.00
IUUC-Tur-126487	Triticum urartu	64.86	0.00e+00	710.00
IUUC-Vda-129989	Verticillium dahliae	28.28	7.00e-50	191.00
IUUC-Vvi-131059	Vitis vinifera	80.53	0.00e+00	882.00
IUUC-Yli-134505	Yarrowia lipolytica	34.44	1.00e-89	323.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence