iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
DNA & RNA Element
microRNA	RAID2
Protein-protein Interaction
IID	iRefIndex	Mentha
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	11806825; 19361490

Classification

Family	E-value	Score	Start	End
E1	6.90e-101	338.7	33	227

Active Site

Position(s)	Description	Evidence
211	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 9    GeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfk 108
           G e+ e+l+++k+l++GagglGcEllKnL+l+G++ i+v+Dmdt++vsnlnrqFLfr++dvgksKAevaa+++++++  ++v+ah+++i+++++e  F++
   Q: 33   GPENFEALQNTKILVIGAGGLGCELLKNLALSGFRTIEVIDMDTIDVSNLNRQFLFRESDVGKSKAEVAAAFVQQRVVGCQVTAHNCRIEDKGQE--FYR 130
           899********************************************************************************************..*** PP
   S: 109  kfdvVvlaldnrearrkvnrlclna.............rvpliesgtlGllGqvrviikgltecyscsd..dppqktiPfctlketpnaaehtiewa 190
           kf++++++ld++ arr++n +++++              +p+i++gt+G++G++rvi++++t+c++c+   +ppq+++P+ct+++tp+ +eh+ie++
   Q: 131  KFSIIICGLDSIPARRWINGMLCDLvlemadgkpdentIIPMIDGGTEGFKGNARVIYPKFTACIDCTLdlYPPQVNFPLCTIAHTPRLPEHCIEYI 227
           *********************************************************************9**************************9 PP

Organism

Caenorhabditis elegans

Functional Description
(View)

Catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba-3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12 (By similarity). Required for cytokinesis and mitotic spindle orientation during early embryogenesis (PubMed:11847342, PubMed:19528325).

Protein Sequence
(Fasta)

MVSVDPLATE RWRSIRRLTD RDSAYKVPWF VPGPENFEAL QNTKILVIGA GGLGCELLKN 60
LALSGFRTIE VIDMDTIDVS NLNRQFLFRE SDVGKSKAEV AAAFVQQRVV GCQVTAHNCR 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AAGGGAATTT GAGAGTAATT GGAACTTCAG TTTGCAGTCT TCAATCTTTA AGAGCTACCG 60
TAACCCTATT TCAGTGAAGA GTCTACTGAT ACCGTTGCGG GCCCATTAAA TACTTAATCC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0217--Developmental protein
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR014929--E2_binding
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR030468--Uba3
IPR033127--UBQ-activ_enz_E1_Cys_AS

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF08825--E2_bind
PF00899--ThiF

SMART

SM01181--E2_bind

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0019781--F:NEDD8 activating enzyme activity
GO:0007275--P:multicellular organism development
GO:0043518--P:negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045116--P:protein neddylation

KEGG

cel:CELE_F11H8.1

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001124	Aegilops tauschii	48.97	4.00e-79	286.00
IUUC-Aml-001578	Ailuropoda melanoleuca	52.80	6.00e-128	449.00
IUUC-Atr-002591	Amborella trichopoda	51.50	2.00e-92	331.00
IUUC-Apl-003671	Anas platyrhynchos	51.52	6.00e-127	446.00
IUUC-Aca-004749	Anolis carolinensis	53.04	5.00e-129	453.00
IUUC-Aly-005747	Arabidopsis lyrata	50.95	2.00e-92	331.00
IUUC-Ath-006623	Arabidopsis thaliana	48.50	7.00e-93	333.00
IUUC-Ago-007934	Ashbya gossypii	42.01	2.00e-64	237.00
IUUC-Acl-008047	Aspergillus clavatus	47.92	1.00e-113	401.00
IUUC-Afl-008615	Aspergillus flavus	40.19	1.00e-63	235.00
IUUC-Afu-008996	Aspergillus fumigatus	47.76	1.00e-103	369.00
IUUC-Ani-009281	Aspergillus nidulans	39.06	4.00e-69	253.00
IUUC-Ang-009539	Aspergillus niger	48.43	1.00e-97	348.00
IUUC-Aor-010151	Aspergillus oryzae	44.39	7.00e-92	329.00
IUUC-Ate-010502	Aspergillus terreus	48.34	4.00e-100	356.00
IUUC-Ame-011837	Astyanax mexicanus	52.10	3.00e-118	417.00
IUUC-Bgr-012068	Blumeria graminis	48.07	3.00e-98	350.00
IUUC-Bta-013003	Bos taurus	53.50	4.00e-129	453.00
IUUC-Bci-013983	Botrytis cinerea	50.77	7.00e-101	359.00
IUUC-Bdi-014429	Brachypodium distachyon	48.27	3.00e-84	304.00
IUUC-Bol-015490	Brassica oleracea	49.00	4.00e-82	296.00
IUUC-Bra-018023	Brassica rapa	52.52	4.00e-81	293.00
IUUC-Cja-019200	Callithrix jacchus	53.49	2.00e-126	444.00
IUUC-Cfa-021038	Canis familiaris	53.50	4.00e-129	453.00
IUUC-Cre-022778	Chlamydomonas reinhardtii	46.25	5.00e-102	363.00
IUUC-Csa-024079	Chlorocebus sabaeus	53.04	2.00e-128	451.00
IUUC-Cho-025052	Choloepus hoffmanni	47.90	4.00e-110	390.00
IUUC-Cin-025113	Ciona intestinalis	50.59	7.00e-121	426.00
IUUC-Csv-026231	Ciona savignyi	51.75	3.00e-126	443.00
IUUC-Cgl-026535	Colletotrichum gloeosporioides	49.76	5.00e-109	386.00
IUUC-Cne-026850	Cryptococcus neoformans	48.23	4.00e-99	353.00
IUUC-Cme-027275	Cyanidioschyzon merolae	36.57	2.00e-66	244.00
IUUC-Dre-028751	Danio rerio	51.75	1.00e-119	421.00
IUUC-Dno-029571	Dasypus novemcinctus	52.80	8.00e-128	449.00
IUUC-Dor-030395	Dipodomys ordii	54.85	1.00e-125	442.00
IUUC-Dse-031329	Dothistroma septosporum	45.37	2.00e-97	348.00
IUUC-Dme-031776	Drosophila melanogaster	52.42	4.00e-119	420.00
IUUC-Ete-032894	Echinops telfairi	55.42	6.00e-43	167.00
IUUC-Eca-034389	Equus caballus	53.50	3.00e-129	453.00
IUUC-Eeu-035152	Erinaceus europaeus	49.50	2.00e-99	355.00
IUUC-Fca-036032	Felis catus	53.50	6.00e-129	452.00
IUUC-Fal-037506	Ficedula albicollis	51.17	6.00e-126	442.00
IUUC-Fox-037871	Fusarium oxysporum	50.69	6.00e-91	326.00
IUUC-Fso-038350	Fusarium solani	50.36	3.00e-103	367.00
IUUC-Gmo-039246	Gadus morhua	51.29	4.00e-118	416.00
IUUC-Ggr-039859	Gaeumannomyces graminis	49.11	1.00e-97	348.00
IUUC-Gga-040843	Gallus gallus	50.93	3.00e-122	430.00
IUUC-Gac-042601	Gasterosteus aculeatus	52.34	1.00e-120	425.00
IUUC-Gma-042744	Glycine max	48.91	7.00e-88	316.00
IUUC-Ggo-044788	Gorilla gorilla	53.15	2.00e-127	447.00
IUUC-Hsa-045849	Homo sapiens	53.27	1.00e-128	451.00
IUUC-Hvu-047819	Hordeum vulgare	54.00	8.00e-83	299.00
IUUC-Itr-047863	Ictidomys tridecemlineatus	53.04	2.00e-128	451.00
IUUC-Kpa-049393	Komagataella pastoris	50.61	5.00e-88	316.00
IUUC-Lch-049471	Latimeria chalumnae	51.40	2.00e-116	411.00
IUUC-Lpe-051297	Leersia perrieri	46.21	1.00e-94	338.00
IUUC-Loc-052910	Lepisosteus oculatus	51.99	4.00e-117	413.00
IUUC-Lma-053181	Leptosphaeria maculans	46.99	5.00e-108	383.00
IUUC-Laf-054314	Loxodonta africana	52.89	6.00e-127	446.00
IUUC-Mcc-054870	Macaca mulatta	53.27	1.00e-128	451.00
IUUC-Meu-056574	Macropus eugenii	44.26	3.00e-61	227.00
IUUC-Mor-056919	Magnaporthe oryzae	46.45	1.00e-101	362.00
IUUC-Mpo-057614	Magnaporthe poae	48.60	7.00e-94	336.00
IUUC-Mtr-058110	Medicago truncatula	50.13	5.00e-85	306.00
IUUC-Mla-058949	Melampsora laricipopulina	46.32	3.00e-96	343.00
IUUC-Mga-059455	Meleagris gallopavo	52.10	8.00e-129	452.00
IUUC-Mvi-060270	Microbotryum violaceum	44.87	1.00e-90	325.00
IUUC-Mmr-061155	Microcebus murinus	53.08	1.00e-123	435.00
IUUC-Mdo-062037	Monodelphis domestica	52.57	1.00e-127	448.00
IUUC-Mmu-063670	Mus musculus	53.27	2.00e-128	451.00
IUUC-Mac-064711	Musa acuminata	49.14	2.00e-87	315.00
IUUC-Mpu-066734	Mustela putorius furo	53.26	8.00e-129	452.00
IUUC-Mlu-067068	Myotis lucifugus	53.50	1.00e-128	452.00
IUUC-Nfi-068349	Neosartorya fischeri	47.53	2.00e-103	367.00
IUUC-Ncr-068626	Neurospora crassa	46.85	4.00e-91	327.00
IUUC-Nle-069366	Nomascus leucogenys	58.87	2.00e-122	431.00
IUUC-Ont-072196	Oreochromis niloticus	52.46	3.00e-119	420.00
IUUC-Oan-073511	Ornithorhynchus anatinus	51.64	3.00e-125	440.00
IUUC-Ocu-073773	Oryctolagus cuniculus	53.50	7.00e-129	452.00
IUUC-Oba-075726	Oryza barthii	45.31	6.00e-82	296.00
IUUC-Obr-076286	Oryza brachyantha	50.14	7.00e-93	332.00
IUUC-Ogu-078473	Oryza glumaepatula	45.31	5.00e-82	296.00
IUUC-Oin-079393	Oryza indica	50.14	1.00e-93	335.00
IUUC-Olo-080446	Oryza longistaminata	46.93	1.00e-85	308.00
IUUC-Ome-081737	Oryza meridionalis	47.88	3.00e-85	307.00
IUUC-Oni-082853	Oryza nivara	45.31	6.00e-82	296.00
IUUC-Opu-083590	Oryza punctata	48.89	6.00e-94	336.00
IUUC-Oru-084184	Oryza rufipogon	45.31	6.00e-82	296.00
IUUC-Osa-085402	Oryza sativa	50.14	1.00e-93	335.00
IUUC-Ola-086671	Oryzias latipes	56.79	7.00e-109	385.00
IUUC-Olu-087738	Ostreococcus lucimarinus	44.24	6.00e-89	319.00
IUUC-Oga-088101	Otolemur garnettii	53.50	8.00e-129	452.00
IUUC-Oar-090293	Ovis aries	52.89	4.00e-127	446.00
IUUC-Ptr-090503	Pan troglodytes	53.27	1.00e-128	451.00
IUUC-Pan-092477	Papio anubis	51.74	2.00e-123	434.00
IUUC-Psi-093442	Pelodiscus sinensis	51.87	2.00e-126	444.00
IUUC-Pma-094247	Petromyzon marinus	50.70	3.00e-115	407.00
IUUC-Pno-094780	Phaeosphaeria nodorum	47.44	2.00e-105	374.00
IUUC-Ppa-095961	Physcomitrella patens	50.36	2.00e-115	407.00
IUUC-Pfo-096846	Poecilia formosa	51.52	3.00e-119	421.00
IUUC-Pab-098060	Pongo abelii	53.04	2.00e-119	421.00
IUUC-Pop-099914	Populus trichocarpa	49.61	2.00e-94	338.00
IUUC-Pca-100500	Procavia capensis	49.28	5.00e-92	330.00
IUUC-Ppe-102008	Prunus persica	49.86	9.00e-85	306.00
IUUC-Pva-103095	Pteropus vampyrus	63.78	2.00e-69	254.00
IUUC-Pgr-103666	Puccinia graminis	46.17	2.00e-97	348.00
IUUC-Ptt-103901	Puccinia triticina	46.17	2.00e-97	348.00
IUUC-Pte-104198	Pyrenophora teres	46.77	3.00e-109	387.00
IUUC-Pyt-104792	Pyrenophora triticirepentis	47.11	2.00e-105	374.00
IUUC-Rno-105446	Rattus norvegicus	53.27	2.00e-128	451.00
IUUC-Sce-106355	Saccharomyces cerevisiae	38.71	3.00e-59	220.00
IUUC-Sha-106664	Sarcophilus harrisii	52.34	2.00e-127	447.00
IUUC-Sja-107982	Schizosaccharomyces japonicus	40.29	5.00e-85	306.00
IUUC-Spo-108345	Schizosaccharomyces pombe	40.33	2.00e-92	331.00
IUUC-Ssl-108540	Sclerotinia sclerotiorum	45.45	4.00e-85	306.00
IUUC-Smo-109133	Selaginella moellendorffii	48.86	4.00e-114	403.00
IUUC-Sit-109724	Setaria italica	49.14	3.00e-84	304.00
IUUC-Sly-110916	Solanum lycopersicum	49.45	1.00e-91	328.00
IUUC-Stu-112236	Solanum tuberosum	49.18	2.00e-91	327.00
IUUC-Sar-113044	Sorex araneus	55.28	2.00e-124	437.00
IUUC-Sbi-114276	Sorghum bicolor	48.66	3.00e-86	311.00
IUUC-Sre-114963	Sporisorium reilianum	47.16	4.00e-99	353.00
IUUC-Ssc-116301	Sus scrofa	52.89	5.00e-127	446.00
IUUC-Tgu-117265	Taeniopygia guttata	51.17	4.00e-126	443.00
IUUC-Tru-117526	Takifugu rubripes	52.21	1.00e-116	411.00
IUUC-Tsy-119483	Tarsius syrichta	54.99	3.00e-125	440.00
IUUC-Tni-120363	Tetraodon nigroviridis	52.57	2.00e-119	421.00
IUUC-Tca-121731	Theobroma cacao	48.14	7.00e-86	309.00
IUUC-Tre-122221	Trichoderma reesei	49.20	2.00e-93	334.00
IUUC-Tvi-122352	Trichoderma virens	48.33	2.00e-101	360.00
IUUC-Tae-123213	Triticum aestivum	48.88	1.00e-85	308.00
IUUC-Tur-126070	Triticum urartu	46.91	8.00e-70	256.00
IUUC-Tme-127199	Tuber melanosporum	50.92	3.00e-99	353.00
IUUC-Tbe-128076	Tupaia belangeri	53.27	2.00e-128	450.00
IUUC-Ttr-128695	Tursiops truncatus	62.24	3.00e-48	184.00
IUUC-Uma-129638	Ustilago maydis	47.69	2.00e-102	364.00
IUUC-Vda-129963	Verticillium dahliae	47.85	5.00e-94	336.00
IUUC-Vpa-130637	Vicugna pacos	46.96	8.00e-106	375.00
IUUC-Vvi-131613	Vitis vinifera	50.65	7.00e-86	309.00
IUUC-Xtr-132924	Xenopus tropicalis	51.05	2.00e-115	407.00
IUUC-Xma-134209	Xiphophorus maculatus	51.64	2.00e-120	424.00
IUUC-Yli-134365	Yarrowia lipolytica	51.21	2.00e-94	337.00
IUUC-Zma-135941	Zea mays	52.20	3.00e-82	298.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence