iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	dbPTM	BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 adaptor/Cullin RING/DCX/DWD	WD_repeat	16648845; 17889668
E3 adaptor/Cullin RING/APC_C/CDC20	CDC20	16648845; 19197151; 17889668; 18538651; 12737808

Classification

Family	E-value	Score	Start	End
E3 adaptor/Cullin RING/DCX/DWD	2.20e-22	76.7	384	416
E3 adaptor/Cullin RING/APC_C/CDC20	7.00e-130	431.2	249	560

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 adaptor/Cullin RING/DCX/DWD

   S: 2    Htdsvtclaf.sd.tllvsGsdDgtikvWDlrt 32
           Ht++v++l++ sd   l+sG++D+t+++WD+rt
   Q: 384  HTGEVCGLSYkSDgLQLASGGNDNTVMIWDTRT 416
           9**********99788**************998 PP

E3 adaptor/Cullin RING/APC_C/CDC20

   S: 1    pekvldapelrddfylnLldWssknvlavaLgssvylwsaktgdvskladleekdlvtsvswieegtllavGtkkgeveiyDveekkkvrtl.tghsarvga 101
           pe++ldap ++ddfylnLl+Ws+knvla+aL++++ylw+a+tgdvs l+d+ e+++++sv+w+++++++++G+++g++ei+Dve+++ +rt+ +g   r+g+
   Q: 249  PERILDAPGFQDDFYLNLLSWSKKNVLAIALDTALYLWNATTGDVSLLTDF-ENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMSLIRTMrSGLGVRIGS 349
           689************************************************.9999************************************99******** PP
   S: 102  LsWnaevlssGsrdgeilhrdvrvkelklstlqahaqevCGlkwnedaklLasGGnDnsvsvwdasssePlkkfkehkaavkaiawsPwqkslLasGGGtaD 203
           LsW ++++++Gsr+gei+++dvr+k++ +st+++h+ evCGl++++d+ +LasGGnDn+v++wd+++s P++++k+h+aavka++w+P+++++LasGGG++D
   Q: 350  LSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLSYKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHTAAVKALSWCPYSPNILASGGGQTD 451
           ****************************************************************************************************** PP
   S: 204  rcikfwntltgalleeidtgsqvcsllwskks.........kelvsthGfaenkitlwkypslskvae.lkgheaRvlslalspdgeaivtaagDetlrfwk 295
           ++i+fwn++tga++ +i+tgsqv+sl+w++++         ke+v+t G++en+i++++y+++ kvae +++heaR+++++lspdg++++t++gDe+l+f+k
   Q: 452  KHIHFWNSITGARVGSINTGSQVSSLHWGQSHtstnggmmnKEIVATGGNPENAISVYNYETKFKVAEvVHAHEARICCSQLSPDGTTLATVGGDENLKFYK 553
           ******************************999***********************************99******************************** PP
   S: 296  lfdkkka 302
           +fd++++
   Q: 554  IFDPRCT 560
           ***9876 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.

Protein Sequence
(Fasta)

MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS SSLNIRNSKR 60
PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD KATFSAYSSR SYPTIGSESV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCCAGAAA GCTCTAGAGA TAAGGGAAAT GCAGCAATTA GCGGTAACCG TTCTGTACTT 60
TCTATTGCGT CCCCAACAAA GCTAAACATA CTATCTTCCG ATTGGTCCAG AAACCAAGGT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0853--WD repeat

Interpro

IPR024977--Apc4_WD40_dom
IPR033010--Cdc20/Fizzy
IPR011047--Quinoprotein_ADH-like_supfam
IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom

PROSITE

PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION

Pfam

PF12894--ANAPC4_WD40

SMART

SM00320--WD40

Gene Ontology

GO:0005680--C:anaphase-promoting complex
GO:0033597--C:mitotic checkpoint complex
GO:0034399--C:nuclear periphery
GO:0010997--F:anaphase-promoting complex binding
GO:0097027--F:ubiquitin-protein transferase activator activity
GO:0031145--P:anaphase-promoting complex-dependent catabolic process
GO:0051301--P:cell division
GO:0007067--P:mitotic nuclear division
GO:0007094--P:mitotic spindle assembly checkpoint
GO:0045842--P:positive regulation of mitotic metaphase/anaphase transition
GO:0045732--P:positive regulation of protein catabolic process
GO:1904668--P:positive regulation of ubiquitin protein ligase activity
GO:0051441--P:positive regulation of ubiquitin-protein ligase activity involved in meiotic cell cycle
GO:0040020--P:regulation of meiotic nuclear division

KEGG

sce:YGL116W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ago-007962	Ashbya gossypii	56.12	1.00e-177	613.00
IUUC-Acl-008043	Aspergillus clavatus	44.69	2.00e-90	324.00
IUUC-Afl-008593	Aspergillus flavus	45.34	4.00e-90	323.00
IUUC-Afu-008793	Aspergillus fumigatus	44.16	2.00e-89	320.00
IUUC-Ani-009158	Aspergillus nidulans	45.34	2.00e-90	324.00
IUUC-Ang-009786	Aspergillus niger	45.23	1.00e-89	322.00
IUUC-Aor-010163	Aspergillus oryzae	45.34	4.00e-90	323.00
IUUC-Ate-010479	Aspergillus terreus	44.81	4.00e-90	323.00
IUUC-Bgr-012059	Blumeria graminis	47.38	2.00e-100	357.00
IUUC-Bci-013607	Botrytis cinerea	46.53	1.00e-98	352.00
IUUC-Cgl-026465	Colletotrichum gloeosporioides	43.39	3.00e-98	350.00
IUUC-Cne-026872	Cryptococcus neoformans	40.89	6.00e-81	292.00
IUUC-Dre-028026	Danio rerio	45.17	8.00e-78	282.00
IUUC-Dse-031391	Dothistroma septosporum	46.30	2.00e-96	344.00
IUUC-Fox-037850	Fusarium oxysporum	47.49	5.00e-98	349.00
IUUC-Fso-038375	Fusarium solani	44.89	3.00e-98	350.00
IUUC-Ggr-039864	Gaeumannomyces graminis	45.16	4.00e-97	346.00
IUUC-Lma-052998	Leptosphaeria maculans	44.62	2.00e-94	338.00
IUUC-Mor-057277	Magnaporthe oryzae	45.53	5.00e-94	336.00
IUUC-Mpo-057624	Magnaporthe poae	44.67	5.00e-94	336.00
IUUC-Mla-059066	Melampsora laricipopulina	39.12	5.00e-77	279.00
IUUC-Mvi-060422	Microbotryum violaceum	40.18	3.00e-81	293.00
IUUC-Nfi-068375	Neosartorya fischeri	44.42	1.00e-89	322.00
IUUC-Ncr-068951	Neurospora crassa	46.03	7.00e-96	342.00
IUUC-Pno-094809	Phaeosphaeria nodorum	45.19	4.00e-95	340.00
IUUC-Pop-098890	Populus trichocarpa	41.78	3.00e-79	286.00
IUUC-Pgr-103627	Puccinia graminis	38.36	5.00e-73	266.00
IUUC-Pte-104142	Pyrenophora teres	44.68	2.00e-93	334.00
IUUC-Pyt-104693	Pyrenophora triticirepentis	44.94	7.00e-94	335.00
IUUC-Sja-107919	Schizosaccharomyces japonicus	49.33	8.00e-104	368.00
IUUC-Spo-108075	Schizosaccharomyces pombe	43.74	2.00e-99	353.00
IUUC-Ssl-108507	Sclerotinia sclerotiorum	46.02	2.00e-97	348.00
IUUC-Sre-114976	Sporisorium reilianum	40.72	3.00e-69	254.00
IUUC-Tru-118184	Takifugu rubripes	39.52	6.00e-66	242.00
IUUC-Tni-120474	Tetraodon nigroviridis	42.01	2.00e-70	257.00
IUUC-Tre-122211	Trichoderma reesei	46.84	2.00e-97	347.00
IUUC-Tvi-122531	Trichoderma virens	46.83	1.00e-95	342.00
IUUC-Tme-126905	Tuber melanosporum	44.72	2.00e-95	341.00
IUUC-Uma-129318	Ustilago maydis	41.92	1.00e-70	258.00
IUUC-Vda-129993	Verticillium dahliae	44.80	2.00e-97	347.00
IUUC-Vvi-131277	Vitis vinifera	40.77	7.00e-78	281.00
IUUC-Yli-134501	Yarrowia lipolytica	42.66	5.00e-100	356.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence